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The map label for this gene is cwlC [H]

Identifier: 83590120

GI number: 83590120

Start: 1315354

End: 1316082

Strand: Reverse

Name: cwlC [H]

Synonym: Moth_1272

Alternate gene names: 83590120

Gene position: 1316082-1315354 (Counterclockwise)

Preceding gene: 83590121

Following gene: 83590119

Centisome position: 50.06

GC content: 62.0

Gene sequence:

>729_bases
ATGACCAGAATTGGTATTGATCCCGGCCACGGTGGCAGCGATCCCGGGGCGGTAGCTGCTGATGGGCTCCAGGAAAAGGA
TGTCACCCTGGCTGTGGGGCTGGATTTACGGCAAAGGTTGCAGGGTGCGGGTCTGGAAGTGATCATGTCCCGTACGGTGG
ACCAGGATGTTTCTTTGGCCAGCCGGGCGGAGCTTTTTAACCGGAAGCAGGTCGACGTGGTGGTTAGCCTGCATGTCAAT
AGCGCTGACAACACCACGGCAAATTACGTCAGCACCTTTATCCTGGCCCCCGGAGGACAGGCGGAGAAGATAGCCCGTTC
CATCCAGCCTGAGCTGGTGGCCGCCACCGGCTGGCCGGACGGCGGCGTACGGGAAGCTAACTTCTATATCCTCAGAGAAA
CTGATGCCCCGGCGGTCCTGGTGGAGATGGGGTTCATCAGCAACCCGGCGACGGCGTCCCTTCTGGCCAAGCCGGGAACC
CGCCAGGCCCTGGCCCTGGCCATTTTTAAGGGCCTGGCGGCCTACCTGGGCCTGCAGGTTAAGGCCCCTGGCGATATTCA
GGGTCACTGGGCCGAGGAGGCCATCCGTCAGGTTTTGGCTACCGGTATCATGGCGGGTTACCCGGACGGCACTTTCCGTC
CCGACCAGCCGGCCACCCGCGCGGAACTGGCCGCCGCCCTAGCCAGACTCCTGGCTAAAATCCCTGGAACCCCGCCGATT
AAAAACTAA

Upstream 100 bases:

>100_bases
GAGAAGGGAAAAGCGTAACTGGTTGAGGACGAACCTTGCCCCCACCGTTTCCAGCGCCTATGATAGACTGCAAGCATATC
ATAGGTTGGGGGTGTTCGAA

Downstream 100 bases:

>100_bases
CTCTCTAGAGCCGCCCATGGGGGCTGACGCTTCCTTCACGAACAATTTAAAAGTAGGCTGAGGTAAGGGAGGTGGAGGGT
ACAGGCGGTAGGTAGCTTGG

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MTRIGIDPGHGGSDPGAVAADGLQEKDVTLAVGLDLRQRLQGAGLEVIMSRTVDQDVSLASRAELFNRKQVDVVVSLHVN
SADNTTANYVSTFILAPGGQAEKIARSIQPELVAATGWPDGGVREANFYILRETDAPAVLVEMGFISNPATASLLAKPGT
RQALALAIFKGLAAYLGLQVKAPGDIQGHWAEEAIRQVLATGIMAGYPDGTFRPDQPATRAELAAALARLLAKIPGTPPI
KN

Sequences:

>Translated_242_residues
MTRIGIDPGHGGSDPGAVAADGLQEKDVTLAVGLDLRQRLQGAGLEVIMSRTVDQDVSLASRAELFNRKQVDVVVSLHVN
SADNTTANYVSTFILAPGGQAEKIARSIQPELVAATGWPDGGVREANFYILRETDAPAVLVEMGFISNPATASLLAKPGT
RQALALAIFKGLAAYLGLQVKAPGDIQGHWAEEAIRQVLATGIMAGYPDGTFRPDQPATRAELAAALARLLAKIPGTPPI
KN
>Mature_241_residues
TRIGIDPGHGGSDPGAVAADGLQEKDVTLAVGLDLRQRLQGAGLEVIMSRTVDQDVSLASRAELFNRKQVDVVVSLHVNS
ADNTTANYVSTFILAPGGQAEKIARSIQPELVAATGWPDGGVREANFYILRETDAPAVLVEMGFISNPATASLLAKPGTR
QALALAIFKGLAAYLGLQVKAPGDIQGHWAEEAIRQVLATGIMAGYPDGTFRPDQPATRAELAAALARLLAKIPGTPPIK
N

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella - in particular of its basal body - and sporulation. CwlC is able to hydrolyze typ

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted, cell wall. Note=Is associated with the mother cell wall of sporulating cells [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=216, Percent_Identity=26.3888888888889, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1790611, Length=94, Percent_Identity=34.0425531914894, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR007730 [H]

Pfam domain/function: PF01520 Amidase_3; PF05036 SPOR [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 25382; Mature: 25251

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRIGIDPGHGGSDPGAVAADGLQEKDVTLAVGLDLRQRLQGAGLEVIMSRTVDQDVSLA
CCCCCCCCCCCCCCCCCEECCCCCCCCEEEEECCCHHHHHCCCCHHHHHHHHCCCHHHHH
SRAELFNRKQVDVVVSLHVNSADNTTANYVSTFILAPGGQAEKIARSIQPELVAATGWPD
HHHHHHCCCCEEEEEEEEECCCCCCHHHHHEEEEECCCCCHHHHHHHCCCCEEEECCCCC
GGVREANFYILRETDAPAVLVEMGFISNPATASLLAKPGTRQALALAIFKGLAAYLGLQV
CCCEECEEEEEEECCCCEEEEEECCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCEEE
KAPGDIQGHWAEEAIRQVLATGIMAGYPDGTFRPDQPATRAELAAALARLLAKIPGTPPI
ECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
KN
CC
>Mature Secondary Structure 
TRIGIDPGHGGSDPGAVAADGLQEKDVTLAVGLDLRQRLQGAGLEVIMSRTVDQDVSLA
CCCCCCCCCCCCCCCCEECCCCCCCCEEEEECCCHHHHHCCCCHHHHHHHHCCCHHHHH
SRAELFNRKQVDVVVSLHVNSADNTTANYVSTFILAPGGQAEKIARSIQPELVAATGWPD
HHHHHHCCCCEEEEEEEEECCCCCCHHHHHEEEEECCCCCHHHHHHHCCCCEEEECCCCC
GGVREANFYILRETDAPAVLVEMGFISNPATASLLAKPGTRQALALAIFKGLAAYLGLQV
CCCEECEEEEEEECCCCEEEEEECCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHCEEE
KAPGDIQGHWAEEAIRQVLATGIMAGYPDGTFRPDQPATRAELAAALARLLAKIPGTPPI
ECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
KN
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8407798; 9384377 [H]