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The map label for this gene is ecaIM [H]

Identifier: 83590061

GI number: 83590061

Start: 1252186

End: 1253598

Strand: Reverse

Name: ecaIM [H]

Synonym: Moth_1213

Alternate gene names: 83590061

Gene position: 1253598-1252186 (Counterclockwise)

Preceding gene: 83590062

Following gene: 83590060

Centisome position: 47.69

GC content: 54.64

Gene sequence:

>1413_bases
TTGGCTAAACGTCTGACAACCCGCGGTTCGGCCTGCCTGGTTTGGGATACAAAAACGGCAGGAGAAATCCATCAGGGTAA
ATATGCCTTACAACCAAAAGAACTGGTTATACCAACGGCAGGAAATGGCCTGGACCGGCAGCAATCCTTATTATCAGAGT
CAGAAGGCAACCTCCTAATCCAGGGCGACAACCTCCAAGCCATGCAGGGGCTCCTGGACAGGGGATATGAAGGTAAAATC
CATCTTATTTATATCGATCCACCTTTTTTCAGCCAGGACAATTACAGCCACCGGGTTCCCCTCGCCGGGACGGCCGCCGG
CCAGGAACGCCGGGTCATAGAACGGGCGGCCTACCGGGACACCTGGAGGGGGGGAATTGATGCCTATCTGGATATGCTGT
ACCCCCGGCTCCAGCTGATGAAAAGGCTACTGGCGTCGAATGGTAGCATTTACGTTCATCTGGATGCCAGTATCAGCCAC
TATAACTGGGTTCGTAATCACGATACCCTGCTTTTCTATGTCAGGGACCCGGCCAGGTTCACCTTTAATAAAGAGTACCT
TCCCTACCCGCCCGGCTACCGGCGACGCGGCAGCCGGGAAACAAAGGGGAAGGGTTATCCTCTGGATGACGTCTGGAATG
CCAATCCATTCGAATTTGAATTAAAAGGGGAAGAAAGCCTGGATTCCATCCAGATCAAAAGCTTTTCCCGGGAGAAGACT
GGCTTTGCCACCCAAAAAAACCTTAGCCTCCTCCGGCGGATTATCAAGGCTTCTTCCAACCCCGGAGACCTGGTGGCTGA
CTTTTTTTGCGGTTCCGGTACCACTCTGGTGGCCGCCGAAGCCCTGGACCGGAAATGGCTGGGTTGTGAAATAGGGTGGA
CCGGTCTTCAGGTAGCCCGCAAGCGTCTGGTAGCGGCAGGAGCCGGCCCCTTCTTTATTGAGGTAGTGCAGCCGGCAAAC
CAGCCAGCCGCCGGTTTAGCGCCTGTCGTTCTCTATCAAAACGACTCGGGAACCGTTGCCCAGAGACTGCCCCGTTTGCT
GGCGAAGGCGACTAGAACCCCGGTGGATAACGGCCTGGAAGAGGTGACCATCGACCTGAAAGGGTATCACCTGCCGGCAA
TTCCGGCCAACCGGTTATCCCGTAAAGGTAGGAGCGACCTGAAGACGGCCACAAGAGCCAGTCGGGAGAATTTCGCCCTT
ATTATCGATTATTGGGCCGTGGACTGGGATTACGACGGGCGTATTTTTAAGAGCAGTTGGCAGGCGTGGCGGGGTTACAG
CAAAAACGAACCGCCAGTACCGGTCCAGGCCCGGGCCATCCTGGCAGCAAAAAAAGAAAGGACCATTGCCGTCCAGGTAG
TTGATATTCTGGGAAATGAGATTCTAACTGTCATAGAAACGGGTAAGCCCTGA

Upstream 100 bases:

>100_bases
GTCAAAGAGATCCTGGCCTCCTGAATGGCTGCCAGGGGAATTCTACCTTGCCTTCCCTGTTTGCATTCCCAGGAACTAAT
AATCAGGAGGCAGGTAACCC

Downstream 100 bases:

>100_bases
CACTGCCAGGGCTCATACATGAGAAGGTGAACATGCGGATAAAAAGTCTGCTGTGAATTTCATGAACAAAGGAAGGAGCT
AAAGTAAAATGGATACCATC

Product: site-specific DNA-methyltransferase (adenine-specific)

Products: NA

Alternate protein names: M.EcaI; Adenine-specific methyltransferase EcaI [H]

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MAKRLTTRGSACLVWDTKTAGEIHQGKYALQPKELVIPTAGNGLDRQQSLLSESEGNLLIQGDNLQAMQGLLDRGYEGKI
HLIYIDPPFFSQDNYSHRVPLAGTAAGQERRVIERAAYRDTWRGGIDAYLDMLYPRLQLMKRLLASNGSIYVHLDASISH
YNWVRNHDTLLFYVRDPARFTFNKEYLPYPPGYRRRGSRETKGKGYPLDDVWNANPFEFELKGEESLDSIQIKSFSREKT
GFATQKNLSLLRRIIKASSNPGDLVADFFCGSGTTLVAAEALDRKWLGCEIGWTGLQVARKRLVAAGAGPFFIEVVQPAN
QPAAGLAPVVLYQNDSGTVAQRLPRLLAKATRTPVDNGLEEVTIDLKGYHLPAIPANRLSRKGRSDLKTATRASRENFAL
IIDYWAVDWDYDGRIFKSSWQAWRGYSKNEPPVPVQARAILAAKKERTIAVQVVDILGNEILTVIETGKP

Sequences:

>Translated_470_residues
MAKRLTTRGSACLVWDTKTAGEIHQGKYALQPKELVIPTAGNGLDRQQSLLSESEGNLLIQGDNLQAMQGLLDRGYEGKI
HLIYIDPPFFSQDNYSHRVPLAGTAAGQERRVIERAAYRDTWRGGIDAYLDMLYPRLQLMKRLLASNGSIYVHLDASISH
YNWVRNHDTLLFYVRDPARFTFNKEYLPYPPGYRRRGSRETKGKGYPLDDVWNANPFEFELKGEESLDSIQIKSFSREKT
GFATQKNLSLLRRIIKASSNPGDLVADFFCGSGTTLVAAEALDRKWLGCEIGWTGLQVARKRLVAAGAGPFFIEVVQPAN
QPAAGLAPVVLYQNDSGTVAQRLPRLLAKATRTPVDNGLEEVTIDLKGYHLPAIPANRLSRKGRSDLKTATRASRENFAL
IIDYWAVDWDYDGRIFKSSWQAWRGYSKNEPPVPVQARAILAAKKERTIAVQVVDILGNEILTVIETGKP
>Mature_469_residues
AKRLTTRGSACLVWDTKTAGEIHQGKYALQPKELVIPTAGNGLDRQQSLLSESEGNLLIQGDNLQAMQGLLDRGYEGKIH
LIYIDPPFFSQDNYSHRVPLAGTAAGQERRVIERAAYRDTWRGGIDAYLDMLYPRLQLMKRLLASNGSIYVHLDASISHY
NWVRNHDTLLFYVRDPARFTFNKEYLPYPPGYRRRGSRETKGKGYPLDDVWNANPFEFELKGEESLDSIQIKSFSREKTG
FATQKNLSLLRRIIKASSNPGDLVADFFCGSGTTLVAAEALDRKWLGCEIGWTGLQVARKRLVAAGAGPFFIEVVQPANQ
PAAGLAPVVLYQNDSGTVAQRLPRLLAKATRTPVDNGLEEVTIDLKGYHLPAIPANRLSRKGRSDLKTATRASRENFALI
IDYWAVDWDYDGRIFKSSWQAWRGYSKNEPPVPVQARAILAAKKERTIAVQVVDILGNEILTVIETGKP

Specific function: This methylase recognizes the double-stranded sequence GGTNACC, causes specific methylation on A-5 on both strands and protects the DNA from cleavage by the EcaI endonuclease [H]

COG id: COG2189

COG function: function code L; Adenine specific DNA methylase Mod

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002295
- InterPro:   IPR002941
- InterPro:   IPR002052 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 52565; Mature: 52434

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKRLTTRGSACLVWDTKTAGEIHQGKYALQPKELVIPTAGNGLDRQQSLLSESEGNLLI
CCCCCCCCCCEEEEECCCCCCCHHCCEEECCCCCEEEECCCCCCHHHHHHHHCCCCCEEE
QGDNLQAMQGLLDRGYEGKIHLIYIDPPFFSQDNYSHRVPLAGTAAGQERRVIERAAYRD
ECCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
TWRGGIDAYLDMLYPRLQLMKRLLASNGSIYVHLDASISHYNWVRNHDTLLFYVRDPARF
HHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEECCCEEEEEEECCCEE
TFNKEYLPYPPGYRRRGSRETKGKGYPLDDVWNANPFEFELKGEESLDSIQIKSFSREKT
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEECCCCHHC
GFATQKNLSLLRRIIKASSNPGDLVADFFCGSGTTLVAAEALDRKWLGCEIGWTGLQVAR
CCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEHHHCCCCCCEECCCHHHHHHH
KRLVAAGAGPFFIEVVQPANQPAAGLAPVVLYQNDSGTVAQRLPRLLAKATRTPVDNGLE
HHHHHCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCE
EVTIDLKGYHLPAIPANRLSRKGRSDLKTATRASRENFALIIDYWAVDWDYDGRIFKSSW
EEEEEECCEECCCCCHHHHHCCCHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCEECCCH
QAWRGYSKNEPPVPVQARAILAAKKERTIAVQVVDILGNEILTVIETGKP
HHHCCCCCCCCCCCHHHHHHEECCCCCEEEEEEEEHHCCCEEEEEECCCC
>Mature Secondary Structure 
AKRLTTRGSACLVWDTKTAGEIHQGKYALQPKELVIPTAGNGLDRQQSLLSESEGNLLI
CCCCCCCCCEEEEECCCCCCCHHCCEEECCCCCEEEECCCCCCHHHHHHHHCCCCCEEE
QGDNLQAMQGLLDRGYEGKIHLIYIDPPFFSQDNYSHRVPLAGTAAGQERRVIERAAYRD
ECCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
TWRGGIDAYLDMLYPRLQLMKRLLASNGSIYVHLDASISHYNWVRNHDTLLFYVRDPARF
HHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEECCCEEEEEEECCCEE
TFNKEYLPYPPGYRRRGSRETKGKGYPLDDVWNANPFEFELKGEESLDSIQIKSFSREKT
EECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEECCCCHHC
GFATQKNLSLLRRIIKASSNPGDLVADFFCGSGTTLVAAEALDRKWLGCEIGWTGLQVAR
CCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEHHHCCCCCCEECCCHHHHHHH
KRLVAAGAGPFFIEVVQPANQPAAGLAPVVLYQNDSGTVAQRLPRLLAKATRTPVDNGLE
HHHHHCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCE
EVTIDLKGYHLPAIPANRLSRKGRSDLKTATRASRENFALIIDYWAVDWDYDGRIFKSSW
EEEEEECCEECCCCCHHHHHCCCHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCEECCCH
QAWRGYSKNEPPVPVQARAILAAKKERTIAVQVVDILGNEILTVIETGKP
HHHCCCCCCCCCCCHHHHHHEECCCCCEEEEEEEEHHCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2183182 [H]