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The map label for this gene is nfsA [C]

Identifier: 83590060

GI number: 83590060

Start: 1251567

End: 1252097

Strand: Reverse

Name: nfsA [C]

Synonym: Moth_1212

Alternate gene names: 83590060

Gene position: 1252097-1251567 (Counterclockwise)

Preceding gene: 83590061

Following gene: 83590058

Centisome position: 47.63

GC content: 57.25

Gene sequence:

>531_bases
ATGGATACCATCCTGAAGTCCATCCTTGCCCGCCGGAGTATCCGCAGGTATACTGGCGAACCTGTCGCCACCGGGGATAT
CAAAGAGCTCCTGGAAGCAGCCATGAGCGCCCCCTCAGCCGGCAATGAGCAGCCCTGGCACTTCGTAGTCATAACTGACC
GGCGGATATTGGATGAGATCCCGAAAGTACATCCTTATTCCCAAATGCTGAAGGAAGCTCCGGCAGCTATCCTGGTTTGC
GGCGATCTGCAACGGCAAAAACATAAGGGTTACTGGGTGCAGGACTGCGCCGCGGCTACCGAGAATATCCTCCTTGCCGC
CCAGGCCAAGGGGCTGGGCACCGTGTGGCTGGGAGTTTACCCCCGCGAAGAAAGGGTTGCCGGTTTACAAAAACTCCTGG
GCCTTCCCGAGCATATAATACCTCTTTCCCTGATTGCCGTTGGGCACCCGGCTGAAAACAAACCCCCGGCCAACCGGTTC
GACCCCTCCAGGGTCCACCTGGACCGGTGGAATAATCCCATGCAACTTTAA

Upstream 100 bases:

>100_bases
GGTAAGCCCTGACACTGCCAGGGCTCATACATGAGAAGGTGAACATGCGGATAAAAAGTCTGCTGTGAATTTCATGAACA
AAGGAAGGAGCTAAAGTAAA

Downstream 100 bases:

>100_bases
GCCCGTCGTAACTAGCACTGGTGAGATACTCCGTGACCAGGTAGTATACGCGTCGAAAGTCGTCATGGGAGGCAGGGGGG
CCATCGTTCGCGAATTACAT

Product: nitroreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MDTILKSILARRSIRRYTGEPVATGDIKELLEAAMSAPSAGNEQPWHFVVITDRRILDEIPKVHPYSQMLKEAPAAILVC
GDLQRQKHKGYWVQDCAAATENILLAAQAKGLGTVWLGVYPREERVAGLQKLLGLPEHIIPLSLIAVGHPAENKPPANRF
DPSRVHLDRWNNPMQL

Sequences:

>Translated_176_residues
MDTILKSILARRSIRRYTGEPVATGDIKELLEAAMSAPSAGNEQPWHFVVITDRRILDEIPKVHPYSQMLKEAPAAILVC
GDLQRQKHKGYWVQDCAAATENILLAAQAKGLGTVWLGVYPREERVAGLQKLLGLPEHIIPLSLIAVGHPAENKPPANRF
DPSRVHLDRWNNPMQL
>Mature_176_residues
MDTILKSILARRSIRRYTGEPVATGDIKELLEAAMSAPSAGNEQPWHFVVITDRRILDEIPKVHPYSQMLKEAPAAILVC
GDLQRQKHKGYWVQDCAAATENILLAAQAKGLGTVWLGVYPREERVAGLQKLLGLPEHIIPLSLIAVGHPAENKPPANRF
DPSRVHLDRWNNPMQL

Specific function: Reduction Of Nitroaromatic Compounds Using NADH. Reduces Nitrofurazone By A Ping-Pong Bi-Bi Mechanism Possibly To Generate A Two-Electron Transfer Product. Major Component Of The Oxygen- Insensitive Nitroreductase Activity In E.Coli. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=165, Percent_Identity=26.6666666666667, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 19608; Mature: 19608

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTILKSILARRSIRRYTGEPVATGDIKELLEAAMSAPSAGNEQPWHFVVITDRRILDEI
CHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECHHHHHHC
PKVHPYSQMLKEAPAAILVCGDLQRQKHKGYWVQDCAAATENILLAAQAKGLGTVWLGVY
CCCCCHHHHHHHCCCEEEEECCHHHHHHCCCCHHHHHHHHHHEEEEECCCCCCEEEEECC
PREERVAGLQKLLGLPEHIIPLSLIAVGHPAENKPPANRFDPSRVHLDRWNNPMQL
CCHHHHHHHHHHHCCCCHHHHHHHHEECCCCCCCCCCCCCCCCEEECCCCCCCCCC
>Mature Secondary Structure
MDTILKSILARRSIRRYTGEPVATGDIKELLEAAMSAPSAGNEQPWHFVVITDRRILDEI
CHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECHHHHHHC
PKVHPYSQMLKEAPAAILVCGDLQRQKHKGYWVQDCAAATENILLAAQAKGLGTVWLGVY
CCCCCHHHHHHHCCCEEEEECCHHHHHHCCCCHHHHHHHHHHEEEEECCCCCCEEEEECC
PREERVAGLQKLLGLPEHIIPLSLIAVGHPAENKPPANRFDPSRVHLDRWNNPMQL
CCHHHHHHHHHHHCCCCHHHHHHHHEECCCCCCCCCCCCCCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]