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The map label for this gene is kdgA [H]

Identifier: 83589285

GI number: 83589285

Start: 422912

End: 423556

Strand: Reverse

Name: kdgA [H]

Synonym: Moth_0420

Alternate gene names: 83589285

Gene position: 423556-422912 (Counterclockwise)

Preceding gene: 83589313

Following gene: 83589284

Centisome position: 16.11

GC content: 52.56

Gene sequence:

>645_bases
ATGCAAAAACTAAAAGTAATGCAGCGAATTATTGACTGCGGCATTGTAGCAGTAGTACGAGCTGAGAATCCTGAGCAGGC
ATTAAAGATTGCCGAGGCGGTTAAAACAGGAGGAGTGGAAGCAATTGAAATTACCTTGACTATACCGGGGGCCCTGGATG
TTATCCGGGAACTGGCCACCACCTACCGGGAAGGTGAAATCTTAATTGGTGCCGGTACAGTCCTGGATGCCACTACCGCC
AGGATGGCCATCCTGGCTGGAGCCGAATTCCTCGTTAGCCCCTGCCTGGACGTGGAGATGGTAAAGACTTGCAACCGCTA
CCAAAAGGTCTGCATGGCCGGGGCGATGTCCATAAGAGAAATAGTCGAAGTTATGGAAGCCGGCAGTGATTTCGTCAAGT
TTTTCCCGGGCAGCGCTTTTGGGCCGGAAATGGTTAAAGCAATCAAAGGGCCATTGCCCCAGGCGCCAATTATACCCACC
GGCGGTGTCAGCCTGGAAAACGTCGCCCAGTGGATCAAAGCCGGATGTGAAGCTGTAGGAGTTGGCGGCGAGCTGATCAA
AGGAGCCAAAACCGGCAATTACGAGCTGGTAAAAGAAACAGCCCGGAAGTTTGTATCCGCCATCCGGGCCGCCAGAGGAT
TATAA

Upstream 100 bases:

>100_bases
AAAAATTTTTGCTAAAACAGAAGGAGTTTTTTACCGGACAGAGAATATATTTATCATAGTAAACCGTTGTTTTATATAAT
GAACCAAGGAGGGTATGGCA

Downstream 100 bases:

>100_bases
GGGGCATCCGGTTACGGCAAATGGTCCATACACTGGTAGTTACTAACAACTGGCCTCTCAGTTAACCGTTGTTGCAACCA
CTAAGGTTGTTTTAGAAGGG

Product: keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MQKLKVMQRIIDCGIVAVVRAENPEQALKIAEAVKTGGVEAIEITLTIPGALDVIRELATTYREGEILIGAGTVLDATTA
RMAILAGAEFLVSPCLDVEMVKTCNRYQKVCMAGAMSIREIVEVMEAGSDFVKFFPGSAFGPEMVKAIKGPLPQAPIIPT
GGVSLENVAQWIKAGCEAVGVGGELIKGAKTGNYELVKETARKFVSAIRAARGL

Sequences:

>Translated_214_residues
MQKLKVMQRIIDCGIVAVVRAENPEQALKIAEAVKTGGVEAIEITLTIPGALDVIRELATTYREGEILIGAGTVLDATTA
RMAILAGAEFLVSPCLDVEMVKTCNRYQKVCMAGAMSIREIVEVMEAGSDFVKFFPGSAFGPEMVKAIKGPLPQAPIIPT
GGVSLENVAQWIKAGCEAVGVGGELIKGAKTGNYELVKETARKFVSAIRAARGL
>Mature_214_residues
MQKLKVMQRIIDCGIVAVVRAENPEQALKIAEAVKTGGVEAIEITLTIPGALDVIRELATTYREGEILIGAGTVLDATTA
RMAILAGAEFLVSPCLDVEMVKTCNRYQKVCMAGAMSIREIVEVMEAGSDFVKFFPGSAFGPEMVKAIKGPLPQAPIIPT
GGVSLENVAQWIKAGCEAVGVGGELIKGAKTGNYELVKETARKFVSAIRAARGL

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI48994954, Length=206, Percent_Identity=33.9805825242718, Blast_Score=110, Evalue=7e-26,
Organism=Escherichia coli, GI1788156, Length=185, Percent_Identity=32.4324324324324, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 22636; Mature: 22636

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKLKVMQRIIDCGIVAVVRAENPEQALKIAEAVKTGGVEAIEITLTIPGALDVIRELAT
CCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHH
TYREGEILIGAGTVLDATTARMAILAGAEFLVSPCLDVEMVKTCNRYQKVCMAGAMSIRE
HHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
IVEVMEAGSDFVKFFPGSAFGPEMVKAIKGPLPQAPIIPTGGVSLENVAQWIKAGCEAVG
HHHHHHCCCHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCHHEEC
VGGELIKGAKTGNYELVKETARKFVSAIRAARGL
CCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQKLKVMQRIIDCGIVAVVRAENPEQALKIAEAVKTGGVEAIEITLTIPGALDVIRELAT
CCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHHH
TYREGEILIGAGTVLDATTARMAILAGAEFLVSPCLDVEMVKTCNRYQKVCMAGAMSIRE
HHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
IVEVMEAGSDFVKFFPGSAFGPEMVKAIKGPLPQAPIIPTGGVSLENVAQWIKAGCEAVG
HHHHHHCCCHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCHHEEC
VGGELIKGAKTGNYELVKETARKFVSAIRAARGL
CCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377 [H]