Click here to switch to the map view.

The map label for this gene is slt [H]

Identifier: 83313497

GI number: 83313497

Start: 4799740

End: 4801569

Strand: Reverse

Name: slt [H]

Synonym: amb4398

Alternate gene names: 83313497

Gene position: 4801569-4799740 (Counterclockwise)

Preceding gene: 83313500

Following gene: 83313496

Centisome position: 96.67

GC content: 68.69

Gene sequence:

>1830_bases
TTGCGCGACAGGCTCCTCCCGGCAGTGATGCTGGCGATCATGGCGGCGACGTCTCCGGCCCTGGCGGGTGAACCCGTCAA
GCCGGCCAAGGTGCGAATCGCCGCCGCCGACGGCGCCCAGACGGCCGCCATCCTTCCCGGTTCCAATCGTGAAGCCCGCG
TGGCCCCCCTGCCCAAGCCGCTGTCGGCGGGCGATGTGGACCTCTACAAGCGGGTGATGGCGCTGCAGACCTCGGGCCAG
TTTGCCGCCGCCGACCGCGAACTGGGCAAGATCCGCGACGAATTGCTGAAGGGCCATGTGCTGGCCCAGCGTCTGATGGC
GCCGGGAGCCAAGCCCAAGTTCCAGGAAATCCGCGCCTGGCTGGCGGATTACGCCGATCTGCCGCAGGCCGAGGCCATTC
ACAAGCTGGCCAAGGGTCTCAAGGGCGTCAAGGGCGGCGGCGCGTTGCGCGCTCCGGCCAAGGGCGGCCTCAAGGGCACC
GGCATTTCCTCCGAGGACGATGGCGCCACCTGGGAAGAGGTGGCCTATGACGTGGATTCGTCGTCCGCCAAGGTGAAGGC
GTTCAAGGCCAAGCTGCGCCAGTCCCTGCGCGACGAGGAGCCGGCCCGCGTCGAGGCCATGATGGCATCGGCCGAGGCGC
AATCCCTGTCCGAGCTAGACTTCGACCGCATGCGGCTGATGGTTGCCGCCGATCACTTCGCCGGCGGCCGCGACGAGCCC
GCCGCGACCCTGGCCGGCCTCTCGGCCGAACGCTCGGGCGAGCAGTTGCCCGCCGCCCATTGGATCGCCGGCCTGGCCCA
GTGGCGCATGGGCAAGCCCGACGTGGCGCGCCGCCATTTCGAGGAAGTGGCCAAGTATTCCGACGGCCAGGATTGGCTGT
CCGCCGCCGGTGCGTTCTGGGCGGCGCGGGCCAATCTGGTGACCCGCCGTCCCGAGGCGGTCAATCACTGGCTGGAACTG
GCCGCCACCTCGTCGCGCACCTATTACGGCCTGCTGGCCCGCGCCGAATTGGGCCATGGTTCCGAATTCTCGTGGGAAGT
GCCGCCCTTCACCGAGGGCGACGCCGATCTGCTGATGCGCGTTTCCGGGGCGCGCCGCGCCCTGGGCCTGCTGCAGCTGG
GCGACCGCGCCGCTGCCGAGGAAGAGCTGCGCAAGCTCTATCCCAACGCCAGCAAGGGTCTGCGGCAATCCATGCTGGTC
ATGGCCTATGCCGGCCAGATGCCGGCCCTGGCGGTCAGTCTGGGCGGCTCGCTGCCGCGAGAGAACGGCCGCCTGCACGA
CGCCGCCGCCTTTCCCGTTCCCGACTGGACGCCCAAGGGCGGCTGGCAGATCGATAAGGCCCTGGTCTTCGCCCTGGTCC
GCCAGGAATCCTCGTTCAATCCCTCCGCCCGGTCGGGGGTCGGTGCCGCCGGCCTGATGCAGCTGATGCCTGCCACCGCC
GCCGCCATCGGTGGCCGGGGCGCCGGTGCCCGCCTGTCCGATCCCGAGCATAATCTCGGCCTCGGCCAGCGCTATGTGGC
CAAGCTGCTGGAGGAAGAGCCGGTCAACGGCAATCTTCTGCTAATGGCGGCCGCCTATAATGCCGGTCCCGGCAATCTGG
GCCGCTGGCTGACCAATATCCGTCACAACGACGACCCGCTGTTGCTGGTGGAAAGCCTGCCGGCCCGCCAGACCCGCGTC
TTCGTGCAGCGGGTGATGACCGGCTATTGGATCTATCAAAGCCGGCTTGGCCAGAAGTCTGACACCCTGGACGCCGTGGC
CTCGGGCGATTGGCCGTTTTACAACGGCCAGGATCCGGTTCATCCCAGGATCCAGAAGGTCTCGCGCTGA

Upstream 100 bases:

>100_bases
CCTCGATTGGTTTTGTCGATAACCCAGTGGTCCGATCCCGGCGCGCGATCCGCGAGCGACGGGCGAATCGGTCCCGCAGC
GAATTCAGAGGAGAACAGCC

Downstream 100 bases:

>100_bases
GTCGCCGAGGCGGCTTGCTCCCTTCGCCATGGCCGGGTCGTCCCGGCCATGAGCGTTTCAGGGGCCATGAGCGTTTCCGG
GGCTGGCCCGTCTTGACTTC

Product: soluble lytic murein transglycosylase and related regulatory protein

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 609; Mature: 609

Protein sequence:

>609_residues
MRDRLLPAVMLAIMAATSPALAGEPVKPAKVRIAAADGAQTAAILPGSNREARVAPLPKPLSAGDVDLYKRVMALQTSGQ
FAAADRELGKIRDELLKGHVLAQRLMAPGAKPKFQEIRAWLADYADLPQAEAIHKLAKGLKGVKGGGALRAPAKGGLKGT
GISSEDDGATWEEVAYDVDSSSAKVKAFKAKLRQSLRDEEPARVEAMMASAEAQSLSELDFDRMRLMVAADHFAGGRDEP
AATLAGLSAERSGEQLPAAHWIAGLAQWRMGKPDVARRHFEEVAKYSDGQDWLSAAGAFWAARANLVTRRPEAVNHWLEL
AATSSRTYYGLLARAELGHGSEFSWEVPPFTEGDADLLMRVSGARRALGLLQLGDRAAAEEELRKLYPNASKGLRQSMLV
MAYAGQMPALAVSLGGSLPRENGRLHDAAAFPVPDWTPKGGWQIDKALVFALVRQESSFNPSARSGVGAAGLMQLMPATA
AAIGGRGAGARLSDPEHNLGLGQRYVAKLLEEEPVNGNLLLMAAAYNAGPGNLGRWLTNIRHNDDPLLLVESLPARQTRV
FVQRVMTGYWIYQSRLGQKSDTLDAVASGDWPFYNGQDPVHPRIQKVSR

Sequences:

>Translated_609_residues
MRDRLLPAVMLAIMAATSPALAGEPVKPAKVRIAAADGAQTAAILPGSNREARVAPLPKPLSAGDVDLYKRVMALQTSGQ
FAAADRELGKIRDELLKGHVLAQRLMAPGAKPKFQEIRAWLADYADLPQAEAIHKLAKGLKGVKGGGALRAPAKGGLKGT
GISSEDDGATWEEVAYDVDSSSAKVKAFKAKLRQSLRDEEPARVEAMMASAEAQSLSELDFDRMRLMVAADHFAGGRDEP
AATLAGLSAERSGEQLPAAHWIAGLAQWRMGKPDVARRHFEEVAKYSDGQDWLSAAGAFWAARANLVTRRPEAVNHWLEL
AATSSRTYYGLLARAELGHGSEFSWEVPPFTEGDADLLMRVSGARRALGLLQLGDRAAAEEELRKLYPNASKGLRQSMLV
MAYAGQMPALAVSLGGSLPRENGRLHDAAAFPVPDWTPKGGWQIDKALVFALVRQESSFNPSARSGVGAAGLMQLMPATA
AAIGGRGAGARLSDPEHNLGLGQRYVAKLLEEEPVNGNLLLMAAAYNAGPGNLGRWLTNIRHNDDPLLLVESLPARQTRV
FVQRVMTGYWIYQSRLGQKSDTLDAVASGDWPFYNGQDPVHPRIQKVSR
>Mature_609_residues
MRDRLLPAVMLAIMAATSPALAGEPVKPAKVRIAAADGAQTAAILPGSNREARVAPLPKPLSAGDVDLYKRVMALQTSGQ
FAAADRELGKIRDELLKGHVLAQRLMAPGAKPKFQEIRAWLADYADLPQAEAIHKLAKGLKGVKGGGALRAPAKGGLKGT
GISSEDDGATWEEVAYDVDSSSAKVKAFKAKLRQSLRDEEPARVEAMMASAEAQSLSELDFDRMRLMVAADHFAGGRDEP
AATLAGLSAERSGEQLPAAHWIAGLAQWRMGKPDVARRHFEEVAKYSDGQDWLSAAGAFWAARANLVTRRPEAVNHWLEL
AATSSRTYYGLLARAELGHGSEFSWEVPPFTEGDADLLMRVSGARRALGLLQLGDRAAAEEELRKLYPNASKGLRQSMLV
MAYAGQMPALAVSLGGSLPRENGRLHDAAAFPVPDWTPKGGWQIDKALVFALVRQESSFNPSARSGVGAAGLMQLMPATA
AAIGGRGAGARLSDPEHNLGLGQRYVAKLLEEEPVNGNLLLMAAAYNAGPGNLGRWLTNIRHNDDPLLLVESLPARQTRV
FVQRVMTGYWIYQSRLGQKSDTLDAVASGDWPFYNGQDPVHPRIQKVSR

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=156, Percent_Identity=35.8974358974359, Blast_Score=98, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 65485; Mature: 65485

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDRLLPAVMLAIMAATSPALAGEPVKPAKVRIAAADGAQTAAILPGSNREARVAPLPKP
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCEECCCCCC
LSAGDVDLYKRVMALQTSGQFAAADRELGKIRDELLKGHVLAQRLMAPGAKPKFQEIRAW
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LADYADLPQAEAIHKLAKGLKGVKGGGALRAPAKGGLKGTGISSEDDGATWEEVAYDVDS
HHHHHCCCHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHCCCC
SSAKVKAFKAKLRQSLRDEEPARVEAMMASAEAQSLSELDFDRMRLMVAADHFAGGRDEP
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCC
AATLAGLSAERSGEQLPAAHWIAGLAQWRMGKPDVARRHFEEVAKYSDGQDWLSAAGAFW
HHHHHCCCHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
AARANLVTRRPEAVNHWLELAATSSRTYYGLLARAELGHGSEFSWEVPPFTEGDADLLMR
HHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEEEHHCCCCCCCCCCCCCCCCCCHHHHHH
VSGARRALGLLQLGDRAAAEEELRKLYPNASKGLRQSMLVMAYAGQMPALAVSLGGSLPR
HHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCC
ENGRLHDAAAFPVPDWTPKGGWQIDKALVFALVRQESSFNPSARSGVGAAGLMQLMPATA
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHHHH
AAIGGRGAGARLSDPEHNLGLGQRYVAKLLEEEPVNGNLLLMAAAYNAGPGNLGRWLTNI
HHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHC
RHNDDPLLLVESLPARQTRVFVQRVMTGYWIYQSRLGQKSDTLDAVASGDWPFYNGQDPV
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCC
HPRIQKVSR
CHHHHHHCC
>Mature Secondary Structure
MRDRLLPAVMLAIMAATSPALAGEPVKPAKVRIAAADGAQTAAILPGSNREARVAPLPKP
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCEECCCCCC
LSAGDVDLYKRVMALQTSGQFAAADRELGKIRDELLKGHVLAQRLMAPGAKPKFQEIRAW
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LADYADLPQAEAIHKLAKGLKGVKGGGALRAPAKGGLKGTGISSEDDGATWEEVAYDVDS
HHHHHCCCHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHCCCC
SSAKVKAFKAKLRQSLRDEEPARVEAMMASAEAQSLSELDFDRMRLMVAADHFAGGRDEP
CCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCC
AATLAGLSAERSGEQLPAAHWIAGLAQWRMGKPDVARRHFEEVAKYSDGQDWLSAAGAFW
HHHHHCCCHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
AARANLVTRRPEAVNHWLELAATSSRTYYGLLARAELGHGSEFSWEVPPFTEGDADLLMR
HHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEEEHHCCCCCCCCCCCCCCCCCCHHHHHH
VSGARRALGLLQLGDRAAAEEELRKLYPNASKGLRQSMLVMAYAGQMPALAVSLGGSLPR
HHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCC
ENGRLHDAAAFPVPDWTPKGGWQIDKALVFALVRQESSFNPSARSGVGAAGLMQLMPATA
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHHHH
AAIGGRGAGARLSDPEHNLGLGQRYVAKLLEEEPVNGNLLLMAAAYNAGPGNLGRWLTNI
HHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHC
RHNDDPLLLVESLPARQTRVFVQRVMTGYWIYQSRLGQKSDTLDAVASGDWPFYNGQDPV
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCC
HPRIQKVSR
CHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]