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The map label for this gene is rpfG [H]

Identifier: 83313326

GI number: 83313326

Start: 4637254

End: 4638549

Strand: Reverse

Name: rpfG [H]

Synonym: amb4227

Alternate gene names: 83313326

Gene position: 4638549-4637254 (Counterclockwise)

Preceding gene: 83313327

Following gene: 83313324

Centisome position: 93.38

GC content: 66.2

Gene sequence:

>1296_bases
GTGGAGATGGCCATGGTCCAGTCCATCAATCTCTTTCGCGCCGTCGACGCCCTGTCCCGGGCGGTGGATCTGGTCGGGAT
CGATGACCTGCAGCACGGCAAGCGCGTCGGTTTCATCGCCTCGCAGGTCGCCAGGGACTTGGGCTGGGACGAGGAGCGGC
GGCGCGACATCCTGTTCGCCTCGTTTCTGCACGATTGCGGCGTGTCGACCACCGACGAGCACGGCCATCTGGTCACCGAG
CTGGAATGGAGCAATGCCGAAGCCCATTGCCTGAGGGGCGAGGGGCTGCTGCGCGACGTGCCGCCGCTGGCCCATCTGGC
GCCCATGGTGCGCTTCCACCACACGCGCTGGAGCCGGCTGGGCGAAATGGGGCTGGAGCCCAGGCTTAGGGAAGACACCA
ACCTGATCTTTCTGGCCGACCGCCTGGACATGCTGCGCGCCGGCATGGATTCCCCGGCGGCGTTCGAGGAGAAGGGCGGC
TTCCTCGGCGTTCTCCGGCGGCACTCGGGAAGCCTGTTCAAGCCGGAACTGGTAGCCGCTCTGGAGAGATGCGCCCAGTC
GGAGGCCTTCTGGTTCACCCAGTCGCCCGAACTTCTGGACACCCATCTGCACGACATGGGCGTCCATCACCTGACCCGGC
CCATCAGTCACCGTGAACTCCGGTCGCTGGCCGTGATGTTTGCCCGGATCATCGATGCCAAGAGCCCCTTCACCGCCCGG
CATTCGGAAAAGGTGGCGGCGCTGGCCGCGCAGATCGCCGGTGACATGGGGCTCGATGCCGAGACCGTGCGTTTGGTGGA
GATCGCCGGCTATCTCCATGACCTCGGCAAGCTCCGGGTGCCGGATATGGTGCTGGAAAAGGCGACGCCCCTCGACCCGC
ACGAGCGGGTGCTGATGAAGCGGCATGCCTTCGACACATACGACGTGCTGTTCCGCCTGTTCGGAGACAACCCCATCGCG
CAATGGGCGGCGTTTCACCACGAAACCCTGAACGGAGAGGGCTATCCATTCCAGCTTTCGGCCCAGGCTCTGCCACTGCC
GGCGCGCATCATCGCCGTGTCGGACATCTTTCAGGCGCTGGTGCAGGAGCGGCCCTACCGCGCCTCCCTGCTGCCCGCCG
AGCTGATGCCCATCATGGAGGGCATGGTTTCGGAGGGGCGGATCGACGGGGCGATTCTCGGCCATGTGGACCAGTCCCTG
GAACGGTACTGGCTGCTGGCGGGAGGCGACGCCTCCAGGCTTGGTTCCGGCCGGGCCGGAGCGGAGATGAGGCCGGGCCG
GACCGTCCGAACTTAA

Upstream 100 bases:

>100_bases
GACGGCAAAAGCAACCCCAAACTCGCCATATGAGCATGGCGCCTTATGTGGATGGACCGCGATCCCGCGACACGGGACAA
TGGCCGGTGGGGATTTTCCG

Downstream 100 bases:

>100_bases
TTACCCGACGATATGGGCGCCGCCGTCGATGAACAGGGTGGTGCCGGTTACCGACTTGGCCAGATCGCTGACCAGATAGG
CGGCGAACGAGCCGCAATCG

Product: HD-GYP domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MEMAMVQSINLFRAVDALSRAVDLVGIDDLQHGKRVGFIASQVARDLGWDEERRRDILFASFLHDCGVSTTDEHGHLVTE
LEWSNAEAHCLRGEGLLRDVPPLAHLAPMVRFHHTRWSRLGEMGLEPRLREDTNLIFLADRLDMLRAGMDSPAAFEEKGG
FLGVLRRHSGSLFKPELVAALERCAQSEAFWFTQSPELLDTHLHDMGVHHLTRPISHRELRSLAVMFARIIDAKSPFTAR
HSEKVAALAAQIAGDMGLDAETVRLVEIAGYLHDLGKLRVPDMVLEKATPLDPHERVLMKRHAFDTYDVLFRLFGDNPIA
QWAAFHHETLNGEGYPFQLSAQALPLPARIIAVSDIFQALVQERPYRASLLPAELMPIMEGMVSEGRIDGAILGHVDQSL
ERYWLLAGGDASRLGSGRAGAEMRPGRTVRT

Sequences:

>Translated_431_residues
MEMAMVQSINLFRAVDALSRAVDLVGIDDLQHGKRVGFIASQVARDLGWDEERRRDILFASFLHDCGVSTTDEHGHLVTE
LEWSNAEAHCLRGEGLLRDVPPLAHLAPMVRFHHTRWSRLGEMGLEPRLREDTNLIFLADRLDMLRAGMDSPAAFEEKGG
FLGVLRRHSGSLFKPELVAALERCAQSEAFWFTQSPELLDTHLHDMGVHHLTRPISHRELRSLAVMFARIIDAKSPFTAR
HSEKVAALAAQIAGDMGLDAETVRLVEIAGYLHDLGKLRVPDMVLEKATPLDPHERVLMKRHAFDTYDVLFRLFGDNPIA
QWAAFHHETLNGEGYPFQLSAQALPLPARIIAVSDIFQALVQERPYRASLLPAELMPIMEGMVSEGRIDGAILGHVDQSL
ERYWLLAGGDASRLGSGRAGAEMRPGRTVRT
>Mature_431_residues
MEMAMVQSINLFRAVDALSRAVDLVGIDDLQHGKRVGFIASQVARDLGWDEERRRDILFASFLHDCGVSTTDEHGHLVTE
LEWSNAEAHCLRGEGLLRDVPPLAHLAPMVRFHHTRWSRLGEMGLEPRLREDTNLIFLADRLDMLRAGMDSPAAFEEKGG
FLGVLRRHSGSLFKPELVAALERCAQSEAFWFTQSPELLDTHLHDMGVHHLTRPISHRELRSLAVMFARIIDAKSPFTAR
HSEKVAALAAQIAGDMGLDAETVRLVEIAGYLHDLGKLRVPDMVLEKATPLDPHERVLMKRHAFDTYDVLFRLFGDNPIA
QWAAFHHETLNGEGYPFQLSAQALPLPARIIAVSDIFQALVQERPYRASLLPAELMPIMEGMVSEGRIDGAILGHVDQSL
ERYWLLAGGDASRLGSGRAGAEMRPGRTVRT

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG2206

COG function: function code T; HD-GYP domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 48125; Mature: 48125

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMAMVQSINLFRAVDALSRAVDLVGIDDLQHGKRVGFIASQVARDLGWDEERRRDILFA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
SFLHDCGVSTTDEHGHLVTELEWSNAEAHCLRGEGLLRDVPPLAHLAPMVRFHHTRWSRL
HHHHHCCCCCCCCCCCEEEEEECCCCCCEEECCCCCHHCCCHHHHHHHHHHHHHHHHHHH
GEMGLEPRLREDTNLIFLADRLDMLRAGMDSPAAFEEKGGFLGVLRRHSGSLFKPELVAA
HHCCCCCCCCCCCCEEEEHHHHHHHHCCCCCCCCHHHCCCEEEHHHHCCCCCCCHHHHHH
LERCAQSEAFWFTQSPELLDTHLHDMGVHHLTRPISHRELRSLAVMFARIIDAKSPFTAR
HHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHH
HSEKVAALAAQIAGDMGLDAETVRLVEIAGYLHDLGKLRVPDMVLEKATPLDPHERVLMK
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHH
RHAFDTYDVLFRLFGDNPIAQWAAFHHETLNGEGYPFQLSAQALPLPARIIAVSDIFQAL
HHCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHH
VQERPYRASLLPAELMPIMEGMVSEGRIDGAILGHVDQSLERYWLLAGGDASRLGSGRAG
HHCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEEECCCHHHCCCCCCC
AEMRPGRTVRT
CCCCCCCCCCC
>Mature Secondary Structure
MEMAMVQSINLFRAVDALSRAVDLVGIDDLQHGKRVGFIASQVARDLGWDEERRRDILFA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHH
SFLHDCGVSTTDEHGHLVTELEWSNAEAHCLRGEGLLRDVPPLAHLAPMVRFHHTRWSRL
HHHHHCCCCCCCCCCCEEEEEECCCCCCEEECCCCCHHCCCHHHHHHHHHHHHHHHHHHH
GEMGLEPRLREDTNLIFLADRLDMLRAGMDSPAAFEEKGGFLGVLRRHSGSLFKPELVAA
HHCCCCCCCCCCCCEEEEHHHHHHHHCCCCCCCCHHHCCCEEEHHHHCCCCCCCHHHHHH
LERCAQSEAFWFTQSPELLDTHLHDMGVHHLTRPISHRELRSLAVMFARIIDAKSPFTAR
HHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHH
HSEKVAALAAQIAGDMGLDAETVRLVEIAGYLHDLGKLRVPDMVLEKATPLDPHERVLMK
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCHHHHHHHH
RHAFDTYDVLFRLFGDNPIAQWAAFHHETLNGEGYPFQLSAQALPLPARIIAVSDIFQAL
HHCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHH
VQERPYRASLLPAELMPIMEGMVSEGRIDGAILGHVDQSLERYWLLAGGDASRLGSGRAG
HHCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHEEEEECCCHHHCCCCCCC
AEMRPGRTVRT
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]