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The map label for this gene is metZ [H]

Identifier: 83313324

GI number: 83313324

Start: 4634935

End: 4636176

Strand: Reverse

Name: metZ [H]

Synonym: amb4225

Alternate gene names: 83313324

Gene position: 4636176-4634935 (Counterclockwise)

Preceding gene: 83313326

Following gene: 83313323

Centisome position: 93.34

GC content: 66.51

Gene sequence:

>1242_bases
ATGGTCGGGGTTTTTGTCTTCGGAGGTCCTATGAACAGCAACGATTCCAAGCCGTCCCGCACTTGGCGCCCCCGCACCAG
GGCCGTGCGCGGTGGATTGTCCCGCACCAATTTCTGCGAGACCTCGGAAGCCGTGTTCATGAATTCCGGCTATGTCTACG
ACAGCGCCGAGGAGGCCGAGGCCAGCTTCGACGGCACCTTGGACCGTATGGTCTATTCCCGCTTCAAGAACCCGACCGTC
GCCGTGTTCGAGCAGCGTCTGGCCGAGATGGAAGGCGCGCCGCCCGGCATGGCCGTGGCGGCCCGGGCCACCGCCTCGGG
GATGGCCGCCGTTCACGCCGCCCTGCTGTGTCAGCTTAGAGCTGGCGACCGGGTGGTGGCCTCCACGGCGCTGTTCGGCT
CGTGTCACTGGATCATCAACGAGTTGCTGCCGCGCTATGGCGTCGAGCGGGTGTTCGTGGACGGCACCGACCTCAAATCC
TGGGAAGCGGCGCTGTCCCGGCCCACCGCCTGTGTCTTCCTGGAGACTCCCTCCAACCCGACCCTGGAGATCATCGACCT
CGCCGCCGTGTGCGAGCTTGCCCACAAGGCCGGCGCCAAGGTGGTGGTGGACAACGTCTTCGCCACGCCCATCCTGCAAA
GCCCGCTGGAACTGGGGGCCGACATCGTCGTCTATTCCGCCACCAAGCACATGGACGGCCAGGGCCGGTGCTTAGGGGGA
GCGGTGCTGGGCTCGCCGGAATTCTGTGCCGACATCCTGGGGCCGTTCCTGCGCAACACCGGCCCGGCCCTCTCGCCGTT
CAACGCCTGGGTGCTGCTCAAGGGGCTCGAGACCCTGGACCTGCGCATGGAGCGTCATTGCGCCAACGCCCTGAAGGTGG
CCCGCTTCCTGGAAGCCCGTCCCGAGGTGGCCCGCGTGCTCTACCCCGGCCTGGAAAGCCATCCCCAGCACGATCTGGCC
AAGCGCCAGATGCGCGGCTGGGGCAGCATCGTCGCGCTGGAGCTCAAGGGCGGCAAGCCGGCGGCCTACAAGCTGCTGAA
CGGTGTCGAGTTGATCGACATCTCCAACAATCTCGGCGACGCCAAGAGCCTGATCACCCATCCCTGGACCACCACCCACC
AGCGCCTGTCGCCCGAGGACAAGCTTTCCATGGGCATTACCGAAGGATTGCTGCGTCTGTCGGTGGGCCTCGAGGACGGC
GACGACCTGGTCGAGGACCTGGGCCGGGCGCTGGAAGGCTAG

Upstream 100 bases:

>100_bases
TGACTCGAAGCGTGGTGATTTGTCCGACCGGATTGCGGCCACGTAAAACATCCCGCTAAAGAGGTCCGAGCGCTCCGCCA
GTCGAGCCCCGGCCCACTCC

Downstream 100 bases:

>100_bases
AACCGATCGATTCTGTCCATGGACAAAGGCCGATACCCACCTGATATGATGGGTATCGGCATTGTTCCGCATGGGGATCT
TCATGTACAGCCAGACCACC

Product: cystathionine beta-lyase/cystathionine gamma-synthase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MVGVFVFGGPMNSNDSKPSRTWRPRTRAVRGGLSRTNFCETSEAVFMNSGYVYDSAEEAEASFDGTLDRMVYSRFKNPTV
AVFEQRLAEMEGAPPGMAVAARATASGMAAVHAALLCQLRAGDRVVASTALFGSCHWIINELLPRYGVERVFVDGTDLKS
WEAALSRPTACVFLETPSNPTLEIIDLAAVCELAHKAGAKVVVDNVFATPILQSPLELGADIVVYSATKHMDGQGRCLGG
AVLGSPEFCADILGPFLRNTGPALSPFNAWVLLKGLETLDLRMERHCANALKVARFLEARPEVARVLYPGLESHPQHDLA
KRQMRGWGSIVALELKGGKPAAYKLLNGVELIDISNNLGDAKSLITHPWTTTHQRLSPEDKLSMGITEGLLRLSVGLEDG
DDLVEDLGRALEG

Sequences:

>Translated_413_residues
MVGVFVFGGPMNSNDSKPSRTWRPRTRAVRGGLSRTNFCETSEAVFMNSGYVYDSAEEAEASFDGTLDRMVYSRFKNPTV
AVFEQRLAEMEGAPPGMAVAARATASGMAAVHAALLCQLRAGDRVVASTALFGSCHWIINELLPRYGVERVFVDGTDLKS
WEAALSRPTACVFLETPSNPTLEIIDLAAVCELAHKAGAKVVVDNVFATPILQSPLELGADIVVYSATKHMDGQGRCLGG
AVLGSPEFCADILGPFLRNTGPALSPFNAWVLLKGLETLDLRMERHCANALKVARFLEARPEVARVLYPGLESHPQHDLA
KRQMRGWGSIVALELKGGKPAAYKLLNGVELIDISNNLGDAKSLITHPWTTTHQRLSPEDKLSMGITEGLLRLSVGLEDG
DDLVEDLGRALEG
>Mature_413_residues
MVGVFVFGGPMNSNDSKPSRTWRPRTRAVRGGLSRTNFCETSEAVFMNSGYVYDSAEEAEASFDGTLDRMVYSRFKNPTV
AVFEQRLAEMEGAPPGMAVAARATASGMAAVHAALLCQLRAGDRVVASTALFGSCHWIINELLPRYGVERVFVDGTDLKS
WEAALSRPTACVFLETPSNPTLEIIDLAAVCELAHKAGAKVVVDNVFATPILQSPLELGADIVVYSATKHMDGQGRCLGG
AVLGSPEFCADILGPFLRNTGPALSPFNAWVLLKGLETLDLRMERHCANALKVARFLEARPEVARVLYPGLESHPQHDLA
KRQMRGWGSIVALELKGGKPAAYKLLNGVELIDISNNLGDAKSLITHPWTTTHQRLSPEDKLSMGITEGLLRLSVGLEDG
DDLVEDLGRALEG

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=327, Percent_Identity=38.5321100917431, Blast_Score=218, Evalue=6e-57,
Organism=Homo sapiens, GI299473759, Length=258, Percent_Identity=40.6976744186046, Blast_Score=191, Evalue=7e-49,
Organism=Homo sapiens, GI25453487, Length=326, Percent_Identity=33.1288343558282, Blast_Score=161, Evalue=8e-40,
Organism=Escherichia coli, GI1790375, Length=388, Percent_Identity=35.3092783505155, Blast_Score=238, Evalue=4e-64,
Organism=Escherichia coli, GI1789383, Length=391, Percent_Identity=26.5984654731458, Blast_Score=121, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17538055, Length=332, Percent_Identity=37.3493975903614, Blast_Score=220, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI17560056, Length=337, Percent_Identity=37.0919881305638, Blast_Score=216, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI71980708, Length=372, Percent_Identity=34.1397849462366, Blast_Score=201, Evalue=7e-52,
Organism=Saccharomyces cerevisiae, GI6323333, Length=414, Percent_Identity=33.5748792270531, Blast_Score=218, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6319307, Length=349, Percent_Identity=36.1031518624642, Blast_Score=202, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6321254, Length=411, Percent_Identity=26.5206812652068, Blast_Score=142, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6321170, Length=294, Percent_Identity=28.2312925170068, Blast_Score=123, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6321904, Length=350, Percent_Identity=28.5714285714286, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI19922554, Length=345, Percent_Identity=38.2608695652174, Blast_Score=241, Evalue=7e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 44605; Mature: 44605

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGVFVFGGPMNSNDSKPSRTWRPRTRAVRGGLSRTNFCETSEAVFMNSGYVYDSAEEAE
CEEEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCEEEEECCEEECCHHHHH
ASFDGTLDRMVYSRFKNPTVAVFEQRLAEMEGAPPGMAVAARATASGMAAVHAALLCQLR
HHCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHC
AGDRVVASTALFGSCHWIINELLPRYGVERVFVDGTDLKSWEAALSRPTACVFLETPSNP
CCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHCCCCEEEEEECCCCC
TLEIIDLAAVCELAHKAGAKVVVDNVFATPILQSPLELGADIVVYSATKHMDGQGRCLGG
CCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCHHHCCCCEEEEECCCCCCCCCCEECC
AVLGSPEFCADILGPFLRNTGPALSPFNAWVLLKGLETLDLRMERHCANALKVARFLEAR
EECCCHHHHHHHHHHHHHCCCCCCCCCCHHHEECCCHHHHHHHHHHHHHHHHHHHHHHCC
PEVARVLYPGLESHPQHDLAKRQMRGWGSIVALELKGGKPAAYKLLNGVELIDISNNLGD
HHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCEEEEEECCCCCC
AKSLITHPWTTTHQRLSPEDKLSMGITEGLLRLSVGLEDGDDLVEDLGRALEG
HHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MVGVFVFGGPMNSNDSKPSRTWRPRTRAVRGGLSRTNFCETSEAVFMNSGYVYDSAEEAE
CEEEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCEEEEECCEEECCHHHHH
ASFDGTLDRMVYSRFKNPTVAVFEQRLAEMEGAPPGMAVAARATASGMAAVHAALLCQLR
HHCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHC
AGDRVVASTALFGSCHWIINELLPRYGVERVFVDGTDLKSWEAALSRPTACVFLETPSNP
CCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHCCCCEEEEEECCCCC
TLEIIDLAAVCELAHKAGAKVVVDNVFATPILQSPLELGADIVVYSATKHMDGQGRCLGG
CCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCHHHCCCCEEEEECCCCCCCCCCEECC
AVLGSPEFCADILGPFLRNTGPALSPFNAWVLLKGLETLDLRMERHCANALKVARFLEAR
EECCCHHHHHHHHHHHHHCCCCCCCCCCHHHEECCCHHHHHHHHHHHHHHHHHHHHHHCC
PEVARVLYPGLESHPQHDLAKRQMRGWGSIVALELKGGKPAAYKLLNGVELIDISNNLGD
HHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCEEEEEECCCCCC
AKSLITHPWTTTHQRLSPEDKLSMGITEGLLRLSVGLEDGDDLVEDLGRALEG
HHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]