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The map label for this gene is cca [H]

Identifier: 83313257

GI number: 83313257

Start: 4571351

End: 4572658

Strand: Reverse

Name: cca [H]

Synonym: amb4158

Alternate gene names: 83313257

Gene position: 4572658-4571351 (Counterclockwise)

Preceding gene: 83313258

Following gene: 83313254

Centisome position: 92.06

GC content: 71.1

Gene sequence:

>1308_bases
ATGAGTAACACCCGCCTTCACCTCAATCCCGGTGATCCCATCGGCCTACTGGGGCCGCAGGACTGGATGGACGCCGCCGA
GACCCGCGCCGTGGTGGCGGCGCTGCAGGCCGAGGGGGCCCGGGTGCGCTTCGTCGGCGGCTGCGTGCGCGACGCCCTGC
TGAAGCGTCCCATCAAGGATGTGGACATCGCCACCCCCGACCCGCCCGAGCGGGTGCTGGCCCTGCTGGACGATGCGGGG
ATTCATGCCATTCCCACCGGCATCGACCACGGCACGGTGACGGCGGTGATCGGCAAGGCCCATTACGAGATCACCACGCT
CAGGGTCGACGTGGAGAGCTTCGGCCGCCACGCCCGCATCGAATACACCGACGACTGGCGGGCCGATGCGTCGCGGCGCG
ACTTCACCATGAACGCCCTGTCGGCCGATCCCGAAGGCAACGTCTACGACCCCTTTGACGGCATCGCCGATATGGCGGCG
GGCCGGGTGCGCTTCGTGGGCGAGCCGGAAAAGCGCATCGAGGAGGATGCGCTGCGCCTGCTGCGCTTCTTCCGCTTCCA
TGCCCATTACGGCCGCGGCATCGCCATGGAGACCCGGGCGCTCAACGCCTGCCGCCGCATGGCCGACCGGCTGAAGGGCC
TGTCGGGCGAGCGGGTGGCGGGGGAGCTGGTCCGCCTGCTGCTGGCCGACGACCCCACGCCGTCCCTGCTGGTCATGCAC
AGCCACGGCATCCTGGCGCACATCCTGCCGGAGGCGAAGGAGTTCGGGCGGCTGCGGACCCTGGCCTGGCTGGAGCGTCG
CGGACTGGCCCGGCCCGAGATCCGTCCCGATTCCATGCGCCGCCTGGCCGCCCTGCTGGCCACCGACCGGGCCGGAGCCC
TGGACCTGGGCGAGCGTCTCAAGCTGTCGGGGGTGCAGGCCAAGCGGGTGGCCGCCATGGCGGCGCCGCGGGTCCACGTG
GGTTTCGCCATGGACGGTCCCGCCACCCGGCGGGCCTTGCGCAAGGTGGGCGGCGACGAGTTCCGTGATCTGGTGATGGT
GGCCTGGGCTGCGCGGCGCTCGACCGAGGCGCGCCCCGATGCCGCCGAGTCCCGGTGCTGGACGGCCTTGCTCGATGCCG
CCCGCGACTGGCAGCCGGTGGAGTTGCCGGTCAAGGGGCGTGATCTGCTGGAATTGGGGGTTCCGCACGGACCGCGGATC
GGCAGCCTTCTGGCCGCGGTGGAGGCGTGGTGGGAAGACCTGGATTACCGTCCTGACCGTTCCCAATGCCTGGACAGGCT
GCGAGGCCTGATGGCGGCGGGGCTATAG

Upstream 100 bases:

>100_bases
GAGGGATCTCCGCTTGGCGCGCCGTTCCCGCGACGGAACCGCCGGTTCAGAATGAGATTCCTCCTCCCGATGGTCGTCGG
AATGACATCGGTGGTCCGGC

Downstream 100 bases:

>100_bases
ACCGAAGGCGCCGGCGAATTCCATGTCGTTCTCAAGCCGCTCGAGCCCGGTGAGCACGCCGCGACCGCTTTTGCGGGCTT
GGTGCAGGATCTGCTCCAGG

Product: tRNA nucleotidyltransferase/poly(A) polymerase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MSNTRLHLNPGDPIGLLGPQDWMDAAETRAVVAALQAEGARVRFVGGCVRDALLKRPIKDVDIATPDPPERVLALLDDAG
IHAIPTGIDHGTVTAVIGKAHYEITTLRVDVESFGRHARIEYTDDWRADASRRDFTMNALSADPEGNVYDPFDGIADMAA
GRVRFVGEPEKRIEEDALRLLRFFRFHAHYGRGIAMETRALNACRRMADRLKGLSGERVAGELVRLLLADDPTPSLLVMH
SHGILAHILPEAKEFGRLRTLAWLERRGLARPEIRPDSMRRLAALLATDRAGALDLGERLKLSGVQAKRVAAMAAPRVHV
GFAMDGPATRRALRKVGGDEFRDLVMVAWAARRSTEARPDAAESRCWTALLDAARDWQPVELPVKGRDLLELGVPHGPRI
GSLLAAVEAWWEDLDYRPDRSQCLDRLRGLMAAGL

Sequences:

>Translated_435_residues
MSNTRLHLNPGDPIGLLGPQDWMDAAETRAVVAALQAEGARVRFVGGCVRDALLKRPIKDVDIATPDPPERVLALLDDAG
IHAIPTGIDHGTVTAVIGKAHYEITTLRVDVESFGRHARIEYTDDWRADASRRDFTMNALSADPEGNVYDPFDGIADMAA
GRVRFVGEPEKRIEEDALRLLRFFRFHAHYGRGIAMETRALNACRRMADRLKGLSGERVAGELVRLLLADDPTPSLLVMH
SHGILAHILPEAKEFGRLRTLAWLERRGLARPEIRPDSMRRLAALLATDRAGALDLGERLKLSGVQAKRVAAMAAPRVHV
GFAMDGPATRRALRKVGGDEFRDLVMVAWAARRSTEARPDAAESRCWTALLDAARDWQPVELPVKGRDLLELGVPHGPRI
GSLLAAVEAWWEDLDYRPDRSQCLDRLRGLMAAGL
>Mature_434_residues
SNTRLHLNPGDPIGLLGPQDWMDAAETRAVVAALQAEGARVRFVGGCVRDALLKRPIKDVDIATPDPPERVLALLDDAGI
HAIPTGIDHGTVTAVIGKAHYEITTLRVDVESFGRHARIEYTDDWRADASRRDFTMNALSADPEGNVYDPFDGIADMAAG
RVRFVGEPEKRIEEDALRLLRFFRFHAHYGRGIAMETRALNACRRMADRLKGLSGERVAGELVRLLLADDPTPSLLVMHS
HGILAHILPEAKEFGRLRTLAWLERRGLARPEIRPDSMRRLAALLATDRAGALDLGERLKLSGVQAKRVAAMAAPRVHVG
FAMDGPATRRALRKVGGDEFRDLVMVAWAARRSTEARPDAAESRCWTALLDAARDWQPVELPVKGRDLLELGVPHGPRIG
SLLAAVEAWWEDLDYRPDRSQCLDRLRGLMAAGL

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=414, Percent_Identity=29.2270531400966, Blast_Score=172, Evalue=5e-43,
Organism=Escherichia coli, GI1789436, Length=221, Percent_Identity=33.4841628959276, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87081691, Length=238, Percent_Identity=28.1512605042017, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71995920, Length=434, Percent_Identity=28.8018433179723, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI281360112, Length=401, Percent_Identity=30.9226932668329, Blast_Score=169, Evalue=4e-42,
Organism=Drosophila melanogaster, GI21357337, Length=401, Percent_Identity=30.9226932668329, Blast_Score=169, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 48004; Mature: 47872

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNTRLHLNPGDPIGLLGPQDWMDAAETRAVVAALQAEGARVRFVGGCVRDALLKRPIKD
CCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHCCCCC
VDIATPDPPERVLALLDDAGIHAIPTGIDHGTVTAVIGKAHYEITTLRVDVESFGRHARI
CCCCCCCCHHHHHHHHHCCCCEEECCCCCCCCEEEEEECCEEEEEEEEEEHHHHCCEEEE
EYTDDWRADASRRDFTMNALSADPEGNVYDPFDGIADMAAGRVRFVGEPEKRIEEDALRL
EECCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHCCCEEECCCHHHHHHHHHHHH
LRFFRFHAHYGRGIAMETRALNACRRMADRLKGLSGERVAGELVRLLLADDPTPSLLVMH
HHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEE
SHGILAHILPEAKEFGRLRTLAWLERRGLARPEIRPDSMRRLAALLATDRAGALDLGERL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHH
KLSGVQAKRVAAMAAPRVHVGFAMDGPATRRALRKVGGDEFRDLVMVAWAARRSTEARPD
HHCCCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
AAESRCWTALLDAARDWQPVELPVKGRDLLELGVPHGPRIGSLLAAVEAWWEDLDYRPDR
HHHHHHHHHHHHHHCCCCCEECCCCCCHHHEECCCCCCHHHHHHHHHHHHHHHCCCCCCH
SQCLDRLRGLMAAGL
HHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNTRLHLNPGDPIGLLGPQDWMDAAETRAVVAALQAEGARVRFVGGCVRDALLKRPIKD
CCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHCCCCC
VDIATPDPPERVLALLDDAGIHAIPTGIDHGTVTAVIGKAHYEITTLRVDVESFGRHARI
CCCCCCCCHHHHHHHHHCCCCEEECCCCCCCCEEEEEECCEEEEEEEEEEHHHHCCEEEE
EYTDDWRADASRRDFTMNALSADPEGNVYDPFDGIADMAAGRVRFVGEPEKRIEEDALRL
EECCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHCCCEEECCCHHHHHHHHHHHH
LRFFRFHAHYGRGIAMETRALNACRRMADRLKGLSGERVAGELVRLLLADDPTPSLLVMH
HHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEE
SHGILAHILPEAKEFGRLRTLAWLERRGLARPEIRPDSMRRLAALLATDRAGALDLGERL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHH
KLSGVQAKRVAAMAAPRVHVGFAMDGPATRRALRKVGGDEFRDLVMVAWAARRSTEARPD
HHCCCHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
AAESRCWTALLDAARDWQPVELPVKGRDLLELGVPHGPRIGSLLAAVEAWWEDLDYRPDR
HHHHHHHHHHHHHHCCCCCEECCCCCCHHHEECCCCCCHHHHHHHHHHHHHHHCCCCCCH
SQCLDRLRGLMAAGL
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA