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The map label for this gene is degU [H]

Identifier: 83312763

GI number: 83312763

Start: 4025696

End: 4026340

Strand: Reverse

Name: degU [H]

Synonym: amb3664

Alternate gene names: 83312763

Gene position: 4026340-4025696 (Counterclockwise)

Preceding gene: 83312764

Following gene: 83312762

Centisome position: 81.06

GC content: 63.1

Gene sequence:

>645_bases
ATGACCATCCGCGTCATGCTGGTCGACGACCACCGCATGTTCCGAGAGGCGCTTCGGGCGCCGCTGGAAGCCGAGTCGGA
CATGGAGATTGTCGCCGAAGCCAATTCCGGTGCCGATACCTTGGCTTCCCTTGATCTGGCCTGTCCGGACGTTCTGGTTC
TCGACATCGGCCTGCCGGACCTCAACGGTATCGAGGTGGCTCGTACGGCGATCAGGCGGCATCCCGACTTGCGTATCGTC
GCCCTGTCGGGCTTTGCCGACCGCATCTATATCGAGGAGATGCTGAAGGCGGGCGCCCATGGTTACGTGGTTAAGTCGTC
CGGAGCTGATGAGTTGGTTTCCGCCATCCGCGCCGTGGCGGGGGGAAGCAGCTTCCTCTCGCCGGAAGCAACCCAGGTCA
TGCTCCGCCATATCCAGTCGGATGCAGACACCGTCGCGCCGCCGCCCAGCGTGCTGGGCAAGCGGGAACGGGAGGTGTTG
GTTCTACTGGCAGGCGGCCTGCGCTCCGCCGAAATTGCCGCCAGCCTCGGCATTGCCGTGGGGACGGTGGAGGTCCATCG
TCGCAATATCAAGCAGAAGCTCGGGATGAATCGTACTGCCGACTTGATCCGCTATGCCGTTCACGAGGGGCTGATTCCCT
CGTGA

Upstream 100 bases:

>100_bases
AGGGAGTCATGGACATCCAAACTCGCCCGGGCGAGGGGACCACCGTCGTCCTGGAAATGCCGTTCGATTACAATGATTCC
GCGCCCACGGAGATGCTGCC

Downstream 100 bases:

>100_bases
TTGCGTAAGGGGAGTCGCGGGAGCCTACCTGTTTCAGGGTAGTCGCCGTGCACATAACCGGGAATTGTCCACGCCTCCGC
TACGCACTACCTTTAGCGAG

Product: response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MTIRVMLVDDHRMFREALRAPLEAESDMEIVAEANSGADTLASLDLACPDVLVLDIGLPDLNGIEVARTAIRRHPDLRIV
ALSGFADRIYIEEMLKAGAHGYVVKSSGADELVSAIRAVAGGSSFLSPEATQVMLRHIQSDADTVAPPPSVLGKREREVL
VLLAGGLRSAEIAASLGIAVGTVEVHRRNIKQKLGMNRTADLIRYAVHEGLIPS

Sequences:

>Translated_214_residues
MTIRVMLVDDHRMFREALRAPLEAESDMEIVAEANSGADTLASLDLACPDVLVLDIGLPDLNGIEVARTAIRRHPDLRIV
ALSGFADRIYIEEMLKAGAHGYVVKSSGADELVSAIRAVAGGSSFLSPEATQVMLRHIQSDADTVAPPPSVLGKREREVL
VLLAGGLRSAEIAASLGIAVGTVEVHRRNIKQKLGMNRTADLIRYAVHEGLIPS
>Mature_213_residues
TIRVMLVDDHRMFREALRAPLEAESDMEIVAEANSGADTLASLDLACPDVLVLDIGLPDLNGIEVARTAIRRHPDLRIVA
LSGFADRIYIEEMLKAGAHGYVVKSSGADELVSAIRAVAGGSSFLSPEATQVMLRHIQSDADTVAPPPSVLGKREREVLV
LLAGGLRSAEIAASLGIAVGTVEVHRRNIKQKLGMNRTADLIRYAVHEGLIPS

Specific function: Regulating factor for the production of extracellular proteases. The N-terminal region acts as an inhibitor, whereas the C-terminal region carries enhancing activity [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=209, Percent_Identity=29.1866028708134, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1790102, Length=203, Percent_Identity=35.4679802955665, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1786747, Length=203, Percent_Identity=28.0788177339901, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788521, Length=215, Percent_Identity=35.3488372093023, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788712, Length=204, Percent_Identity=26.9607843137255, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787473, Length=182, Percent_Identity=30.7692307692308, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788546, Length=218, Percent_Identity=25.2293577981651, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22935; Mature: 22804

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIRVMLVDDHRMFREALRAPLEAESDMEIVAEANSGADTLASLDLACPDVLVLDIGLPD
CEEEEEEECCHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCEEEEEECCCC
LNGIEVARTAIRRHPDLRIVALSGFADRIYIEEMLKAGAHGYVVKSSGADELVSAIRAVA
CCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHH
GGSSFLSPEATQVMLRHIQSDADTVAPPPSVLGKREREVLVLLAGGLRSAEIAASLGIAV
CCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHH
GTVEVHRRNIKQKLGMNRTADLIRYAVHEGLIPS
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TIRVMLVDDHRMFREALRAPLEAESDMEIVAEANSGADTLASLDLACPDVLVLDIGLPD
EEEEEEECCHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCEEEEEECCCC
LNGIEVARTAIRRHPDLRIVALSGFADRIYIEEMLKAGAHGYVVKSSGADELVSAIRAVA
CCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHH
GGSSFLSPEATQVMLRHIQSDADTVAPPPSVLGKREREVLVLLAGGLRSAEIAASLGIAV
CCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHH
GTVEVHRRNIKQKLGMNRTADLIRYAVHEGLIPS
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7765823 [H]