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The map label for this gene is pleD [H]

Identifier: 83312400

GI number: 83312400

Start: 3582241

End: 3583641

Strand: Reverse

Name: pleD [H]

Synonym: amb3301

Alternate gene names: 83312400

Gene position: 3583641-3582241 (Counterclockwise)

Preceding gene: 83312401

Following gene: 83312398

Centisome position: 72.15

GC content: 59.67

Gene sequence:

>1401_bases
ATGCGCTCGCTCTCGACCATCACCTGGCTTGTGGTCTTGCTGCTTTGCACGGCAGCGTGGTCGGCGGGGGCTGCCGAGTT
GACCGATAGCGACCGCGCCTATGCGGCTTCGCTGGGAAAGGTGAGTTTTTGCGTTGATCCGGACTGGGAGCCATTCGAGA
TTATTTCACCGGCAGGGCAGCATCAAGGCATCGCTGCCGACCTACTTCGACTGGTGACCGGCCGGGCGGGGTTGAGCCTG
GAGTTGGTGCCGACCACGGATTGGGATGAAAGCATCGCCACCTCCAAAGCCGGTCAGTGCACCTTGCTGAGTTTTCTCAA
CCAGACTCCGAAGCGGGACGAGTGGCTCTTGTTCACCGAACCCCTGTTCACCGACAGCAACGTCTTCATCACCCGCGAGG
AACATCCTTTCATCATTGATCCGGCGATCCTGAGCGGGGAAAGTATCGCCCTGCCTAGCGGTACCTCGATGGAAGAACGC
ATCCGTCGGGACTATCCAAATCTGCGCGTAATCATCACTGACAGTGAAAAATCGGCCATCGATATGGTCTCGACGAGACA
GGCATCGATGACTCTGCGCTCGCTGATTGTCGCGGCCTACACCATCCGGAAGGAAGGGCTGTTCAATCTGAAGATTGCCG
GCCAGATGCCCAATTACTCAAACAATCTGCGAATCGGAGTCCTCCGCGACCAGCCGAGGCTGCGCGATATCCTGAACAAG
GCCATCGCCACGATCACCCCCACCGAACGGGGCCAGATCATCAACCAGCACGTCGCAATTCAGGTCCAGACCGGCATTGA
CTATGCCCTGACCATCAAGATCGCACTGGGCCTGAGCGCCGTCCTGGCGGTGGTGGCCTACTGGGGATGGCGGATGCGCA
AGCTGAGCCGGGAACTCGACAGGCTTTCACAGACCGACAACCTGACTAACCTGCCCAACCGGACCCGACTGAACCTGCAG
TTTCCCATCGAATTGGCCCGGGCCCGCCGCCTAGGGCAGGGGCTGTCGCTGATCATGATCGACGTCGATCACTTCAAACG
GATCAACGATGAATTCGGGCATCTGGTCGGAGACAAAGTCCTGATCGCCATGGCGGATCTCGTTCGCCGCTCGGTGCGTG
CCATCGACCTGCCTGCCCGCTGGGGTGGCGAGGAACTTGTGGTACTGTGCCCGGATTCCCACCTGGATCAGGCCCTGATC
CTTGCTGAACGTATCCGCCAGGCGGTGCGCGATCATGACTTCGACACTGGTCGGCGACAGACTGTCAGCATAGGTGTCGC
TACCCTCGGACCATCTGACGATATCGACCAATTGCTGAACCGCGCGGATCAGGCCCTCTACATAGGCAAGAGCAGCGGTC
GCGACCGGGTCTGCTCGGAAGCCGACCTGACAGACACCTGA

Upstream 100 bases:

>100_bases
TCGGCATCGAATATGGCTACGAGGCATTCACCGCCAGCCCAACCGGAACCGGAACCGCCTCGCGGGTGCTGGGCGTGGTG
AAATACGGATACTGACGCCC

Downstream 100 bases:

>100_bases
GGCCGGTCCGTGATCCCTTGCAATCACGGCCGCCAGCCCTGTTAGGCCGCCGTCAATTGGCCAAGTGCTCGGCGAGGGTC
CGGGTGGGCATGGGATGGAT

Product: response regulator

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MRSLSTITWLVVLLLCTAAWSAGAAELTDSDRAYAASLGKVSFCVDPDWEPFEIISPAGQHQGIAADLLRLVTGRAGLSL
ELVPTTDWDESIATSKAGQCTLLSFLNQTPKRDEWLLFTEPLFTDSNVFITREEHPFIIDPAILSGESIALPSGTSMEER
IRRDYPNLRVIITDSEKSAIDMVSTRQASMTLRSLIVAAYTIRKEGLFNLKIAGQMPNYSNNLRIGVLRDQPRLRDILNK
AIATITPTERGQIINQHVAIQVQTGIDYALTIKIALGLSAVLAVVAYWGWRMRKLSRELDRLSQTDNLTNLPNRTRLNLQ
FPIELARARRLGQGLSLIMIDVDHFKRINDEFGHLVGDKVLIAMADLVRRSVRAIDLPARWGGEELVVLCPDSHLDQALI
LAERIRQAVRDHDFDTGRRQTVSIGVATLGPSDDIDQLLNRADQALYIGKSSGRDRVCSEADLTDT

Sequences:

>Translated_466_residues
MRSLSTITWLVVLLLCTAAWSAGAAELTDSDRAYAASLGKVSFCVDPDWEPFEIISPAGQHQGIAADLLRLVTGRAGLSL
ELVPTTDWDESIATSKAGQCTLLSFLNQTPKRDEWLLFTEPLFTDSNVFITREEHPFIIDPAILSGESIALPSGTSMEER
IRRDYPNLRVIITDSEKSAIDMVSTRQASMTLRSLIVAAYTIRKEGLFNLKIAGQMPNYSNNLRIGVLRDQPRLRDILNK
AIATITPTERGQIINQHVAIQVQTGIDYALTIKIALGLSAVLAVVAYWGWRMRKLSRELDRLSQTDNLTNLPNRTRLNLQ
FPIELARARRLGQGLSLIMIDVDHFKRINDEFGHLVGDKVLIAMADLVRRSVRAIDLPARWGGEELVVLCPDSHLDQALI
LAERIRQAVRDHDFDTGRRQTVSIGVATLGPSDDIDQLLNRADQALYIGKSSGRDRVCSEADLTDT
>Mature_466_residues
MRSLSTITWLVVLLLCTAAWSAGAAELTDSDRAYAASLGKVSFCVDPDWEPFEIISPAGQHQGIAADLLRLVTGRAGLSL
ELVPTTDWDESIATSKAGQCTLLSFLNQTPKRDEWLLFTEPLFTDSNVFITREEHPFIIDPAILSGESIALPSGTSMEER
IRRDYPNLRVIITDSEKSAIDMVSTRQASMTLRSLIVAAYTIRKEGLFNLKIAGQMPNYSNNLRIGVLRDQPRLRDILNK
AIATITPTERGQIINQHVAIQVQTGIDYALTIKIALGLSAVLAVVAYWGWRMRKLSRELDRLSQTDNLTNLPNRTRLNLQ
FPIELARARRLGQGLSLIMIDVDHFKRINDEFGHLVGDKVLIAMADLVRRSVRAIDLPARWGGEELVVLCPDSHLDQALI
LAERIRQAVRDHDFDTGRRQTVSIGVATLGPSDDIDQLLNRADQALYIGKSSGRDRVCSEADLTDT

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=168, Percent_Identity=41.6666666666667, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI145693134, Length=171, Percent_Identity=37.4269005847953, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI87082007, Length=162, Percent_Identity=40.1234567901235, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787262, Length=167, Percent_Identity=34.7305389221557, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1788085, Length=186, Percent_Identity=32.7956989247312, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1788381, Length=169, Percent_Identity=36.094674556213, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1787816, Length=157, Percent_Identity=35.6687898089172, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787802, Length=132, Percent_Identity=39.3939393939394, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1786584, Length=169, Percent_Identity=30.7692307692308, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87081974, Length=273, Percent_Identity=28.2051282051282, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787541, Length=173, Percent_Identity=30.0578034682081, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI87081977, Length=165, Percent_Identity=31.5151515151515, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1787056, Length=227, Percent_Identity=26.8722466960352, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788713, Length=227, Percent_Identity=21.5859030837004, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 51792; Mature: 51792

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSLSTITWLVVLLLCTAAWSAGAAELTDSDRAYAASLGKVSFCVDPDWEPFEIISPAGQ
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCEEEEECCCCCCCEEECCCCC
HQGIAADLLRLVTGRAGLSLELVPTTDWDESIATSKAGQCTLLSFLNQTPKRDEWLLFTE
CCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHCCCCCHHHHHHHCCCCCCCCEEEEEC
PLFTDSNVFITREEHPFIIDPAILSGESIALPSGTSMEERIRRDYPNLRVIITDSEKSAI
CCCCCCCEEEEECCCCEEECCHHCCCCEEECCCCCCHHHHHHHCCCCEEEEEECCCCCHH
DMVSTRQASMTLRSLIVAAYTIRKEGLFNLKIAGQMPNYSNNLRIGVLRDQPRLRDILNK
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEECCCHHHHHHHHH
AIATITPTERGQIINQHVAIQVQTGIDYALTIKIALGLSAVLAVVAYWGWRMRKLSRELD
HHHCCCCCCCCCEECCEEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLSQTDNLTNLPNRTRLNLQFPIELARARRLGQGLSLIMIDVDHFKRINDEFGHLVGDKV
HHHHCCCCCCCCCCEEEEEECCHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHH
LIAMADLVRRSVRAIDLPARWGGEELVVLCPDSHLDQALILAERIRQAVRDHDFDTGRRQ
HHHHHHHHHHHHHEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCE
TVSIGVATLGPSDDIDQLLNRADQALYIGKSSGRDRVCSEADLTDT
EEEEEEEECCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRSLSTITWLVVLLLCTAAWSAGAAELTDSDRAYAASLGKVSFCVDPDWEPFEIISPAGQ
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCEEEEECCCCCCCEEECCCCC
HQGIAADLLRLVTGRAGLSLELVPTTDWDESIATSKAGQCTLLSFLNQTPKRDEWLLFTE
CCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHCCCCCHHHHHHHCCCCCCCCEEEEEC
PLFTDSNVFITREEHPFIIDPAILSGESIALPSGTSMEERIRRDYPNLRVIITDSEKSAI
CCCCCCCEEEEECCCCEEECCHHCCCCEEECCCCCCHHHHHHHCCCCEEEEEECCCCCHH
DMVSTRQASMTLRSLIVAAYTIRKEGLFNLKIAGQMPNYSNNLRIGVLRDQPRLRDILNK
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEECCCHHHHHHHHH
AIATITPTERGQIINQHVAIQVQTGIDYALTIKIALGLSAVLAVVAYWGWRMRKLSRELD
HHHCCCCCCCCCEECCEEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLSQTDNLTNLPNRTRLNLQFPIELARARRLGQGLSLIMIDVDHFKRINDEFGHLVGDKV
HHHHCCCCCCCCCCEEEEEECCHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHH
LIAMADLVRRSVRAIDLPARWGGEELVVLCPDSHLDQALILAERIRQAVRDHDFDTGRRQ
HHHHHHHHHHHHHEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCE
TVSIGVATLGPSDDIDQLLNRADQALYIGKSSGRDRVCSEADLTDT
EEEEEEEECCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA