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The map label for this gene is serA [H]

Identifier: 83312292

GI number: 83312292

Start: 3459694

End: 3461274

Strand: Reverse

Name: serA [H]

Synonym: amb3193

Alternate gene names: 83312292

Gene position: 3461274-3459694 (Counterclockwise)

Preceding gene: 162319784

Following gene: 83312291

Centisome position: 69.68

GC content: 65.15

Gene sequence:

>1581_bases
ATGCCCAAGGTTCTCATCAGCGACAAGTTGAGCCCCGCCGCCGTTCAGATCTTCAAGGATCGCGGCATCGAAACCGACGT
GAAGACCGGCCTCGCTCCCGACGAGCTGAAGGCCATCATCGGCCAGTATGACGGTCTGGCCATCCGTTCCAACACCAAGG
TCACCACCGAGATCCTGGCCGCCGCCACCAACCTCAAGGTTGTCGGCCGCGCCGGTATCGGCGTCGACAACGTGGACGTG
CCCGCCGCCACGGCGCGCGGCATCGTGGTGATGAACACCCCCTTCGGCAATTCCATCACTACCGCCGAGCACGCCATCGC
CATGATGTTCGCCCTGGCCCGCGAGATTCCCGAGGCCAATGCCAGCACCCACGCCGGCAAGTGGGAAAAGAACCGCTTCA
TGGGCGTCGAGCTGACCGGCAAGGTGCTGGGCATCGTCGGCTGCGGCAATATCGGCGCCATCGTCGCCGACCGCGCTCAG
GGCCTGCGCATGCGCGTCATCGCCTATGACCCCTTCCTCTCCGTCGAGCGCGCCAAGGAGCTGAACGTCGAGAAGGTGGA
ACTGGACGAACTGTTCCCCCGCGCCGACTTCATCACGCTGCACACGCCCTTGACCGACGCCACCCGCAACATCATCGACG
CCAAGGCCATGGCCAAGATGAAGAAGGGCGTGCGCATCATCAATTGCGCCCGTGGCGGCCTGGTGGTCGAGGAAGACCTG
CTGGCGGCCCTGGAGTCCGGCCAGGTGGCCGGTGCCGCGCTGGACGTCTTCAAGACCGAACCCGCCAAGGAAAACGCCCT
GTTCGGCAATCCCAAGGTGGTCTGCACTCCGCATCTGGGCGCCTCCACCTCCGAGGCCCAGGAAAACGTTGCCTTGCAGG
TGGCCGAGCAGATGGCCGATTACCTGCTGACCGGCGCCGTCACCAACGCGCTGAACATCCCGTCGGTGTCGGCCGAGGAC
GCGCCCAAGCTGCGCCCCTACATGACGCTGGCCAACCAGATCGGCAGCTTCGCCGGCCAGTTGACCGAGACCGGCATCAA
GGACGTGAAGATCGAGTATCTGGGCCACGTGGCCTCGCTCAACACCAAGCCGCTGACCGCCATGGTCCTCGAAGGGCTGC
TCAAGCCCATGAACGAGGCCGTCAACATGGTCAACGCCCCCGTGGTGGCCAAGGAGCGCAACATCAAGGTCTCCGAGGTC
AAGTCCGAGTCCGAGGGTGACTATCACACCCTGGTGCGCCTGACCGTCACCACCGACAAGCGCGAGCGTTCGGTGGCCGG
CACCTTGTTCGGCGGCAACAAGGCCCGCGTGGTGGAGATCAAGGGCATCTCCATCGAGGCCGAGCTGGGCCAGAACATGC
TTTATGTCACCAACCAGGACAAGCCGGGCTTCATCGGCGCCCTGGGCTCCCTGCTGGGCGCCAACGGCGTCAACATCGCC
ACCTTCCACCTCGGCCGTTCCGAGGCGGGCGGCGACGCCATCCTGCTGACCCAGGTGGACCAGCCGGTCTCCAACGACCT
GCTGGAAAAGGTCCGGGCCCTGCCCCAGGTGGTGCAGGCCAAGTTCCTGACCTTCGCCTGA

Upstream 100 bases:

>100_bases
AAGGTCTCCTAAGCCGAAAACACCGTGAACCGGGCGCCGCCTTTCAAAAAGGCGGCGCCCGAACTCCATGTGAGCCCTTT
TCCCACTCGAGGAAGTCCAG

Downstream 100 bases:

>100_bases
TCCCGGTTCATTCCTGATACCGGCAAGGGGGGGCGCGGCGGTGACGCCGCGGCCCCCAGCTTTTTGATGCCCTTCCCCGT
GTCTGGACGAAGCCGCACGC

Product: D-3-phosphoglycerate dehydrogenase

Products: NA

Alternate protein names: PGDH [H]

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MPKVLISDKLSPAAVQIFKDRGIETDVKTGLAPDELKAIIGQYDGLAIRSNTKVTTEILAAATNLKVVGRAGIGVDNVDV
PAATARGIVVMNTPFGNSITTAEHAIAMMFALAREIPEANASTHAGKWEKNRFMGVELTGKVLGIVGCGNIGAIVADRAQ
GLRMRVIAYDPFLSVERAKELNVEKVELDELFPRADFITLHTPLTDATRNIIDAKAMAKMKKGVRIINCARGGLVVEEDL
LAALESGQVAGAALDVFKTEPAKENALFGNPKVVCTPHLGASTSEAQENVALQVAEQMADYLLTGAVTNALNIPSVSAED
APKLRPYMTLANQIGSFAGQLTETGIKDVKIEYLGHVASLNTKPLTAMVLEGLLKPMNEAVNMVNAPVVAKERNIKVSEV
KSESEGDYHTLVRLTVTTDKRERSVAGTLFGGNKARVVEIKGISIEAELGQNMLYVTNQDKPGFIGALGSLLGANGVNIA
TFHLGRSEAGGDAILLTQVDQPVSNDLLEKVRALPQVVQAKFLTFA

Sequences:

>Translated_526_residues
MPKVLISDKLSPAAVQIFKDRGIETDVKTGLAPDELKAIIGQYDGLAIRSNTKVTTEILAAATNLKVVGRAGIGVDNVDV
PAATARGIVVMNTPFGNSITTAEHAIAMMFALAREIPEANASTHAGKWEKNRFMGVELTGKVLGIVGCGNIGAIVADRAQ
GLRMRVIAYDPFLSVERAKELNVEKVELDELFPRADFITLHTPLTDATRNIIDAKAMAKMKKGVRIINCARGGLVVEEDL
LAALESGQVAGAALDVFKTEPAKENALFGNPKVVCTPHLGASTSEAQENVALQVAEQMADYLLTGAVTNALNIPSVSAED
APKLRPYMTLANQIGSFAGQLTETGIKDVKIEYLGHVASLNTKPLTAMVLEGLLKPMNEAVNMVNAPVVAKERNIKVSEV
KSESEGDYHTLVRLTVTTDKRERSVAGTLFGGNKARVVEIKGISIEAELGQNMLYVTNQDKPGFIGALGSLLGANGVNIA
TFHLGRSEAGGDAILLTQVDQPVSNDLLEKVRALPQVVQAKFLTFA
>Mature_525_residues
PKVLISDKLSPAAVQIFKDRGIETDVKTGLAPDELKAIIGQYDGLAIRSNTKVTTEILAAATNLKVVGRAGIGVDNVDVP
AATARGIVVMNTPFGNSITTAEHAIAMMFALAREIPEANASTHAGKWEKNRFMGVELTGKVLGIVGCGNIGAIVADRAQG
LRMRVIAYDPFLSVERAKELNVEKVELDELFPRADFITLHTPLTDATRNIIDAKAMAKMKKGVRIINCARGGLVVEEDLL
AALESGQVAGAALDVFKTEPAKENALFGNPKVVCTPHLGASTSEAQENVALQVAEQMADYLLTGAVTNALNIPSVSAEDA
PKLRPYMTLANQIGSFAGQLTETGIKDVKIEYLGHVASLNTKPLTAMVLEGLLKPMNEAVNMVNAPVVAKERNIKVSEVK
SESEGDYHTLVRLTVTTDKRERSVAGTLFGGNKARVVEIKGISIEAELGQNMLYVTNQDKPGFIGALGSLLGANGVNIAT
FHLGRSEAGGDAILLTQVDQPVSNDLLEKVRALPQVVQAKFLTFA

Specific function: Serine biosynthesis; first step. [C]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=402, Percent_Identity=43.5323383084577, Blast_Score=331, Evalue=1e-90,
Organism=Homo sapiens, GI145580578, Length=284, Percent_Identity=32.3943661971831, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI4557499, Length=284, Percent_Identity=32.3943661971831, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI4557497, Length=274, Percent_Identity=32.4817518248175, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI61743967, Length=274, Percent_Identity=32.4817518248175, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI145580575, Length=275, Percent_Identity=33.0909090909091, Blast_Score=129, Evalue=7e-30,
Organism=Homo sapiens, GI6912396, Length=272, Percent_Identity=32.7205882352941, Blast_Score=121, Evalue=2e-27,
Organism=Escherichia coli, GI1789279, Length=297, Percent_Identity=39.7306397306397, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI87082289, Length=286, Percent_Identity=34.6153846153846, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI1787645, Length=196, Percent_Identity=34.6938775510204, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI87081824, Length=266, Percent_Identity=28.5714285714286, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1788660, Length=304, Percent_Identity=29.9342105263158, Blast_Score=79, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17532191, Length=300, Percent_Identity=48.6666666666667, Blast_Score=290, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI25147481, Length=257, Percent_Identity=36.1867704280156, Blast_Score=152, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6322116, Length=330, Percent_Identity=35.1515151515151, Blast_Score=208, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6320925, Length=331, Percent_Identity=34.7432024169184, Blast_Score=207, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6324055, Length=269, Percent_Identity=29.7397769516729, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6324964, Length=273, Percent_Identity=28.9377289377289, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6325144, Length=115, Percent_Identity=39.1304347826087, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324980, Length=199, Percent_Identity=27.6381909547739, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19921140, Length=300, Percent_Identity=49, Blast_Score=288, Evalue=7e-78,
Organism=Drosophila melanogaster, GI24585516, Length=289, Percent_Identity=32.5259515570934, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI28574286, Length=245, Percent_Identity=32.6530612244898, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24585514, Length=264, Percent_Identity=30.3030303030303, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI28574282, Length=264, Percent_Identity=30.3030303030303, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI45551003, Length=264, Percent_Identity=30.3030303030303, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24646446, Length=259, Percent_Identity=33.5907335907336, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24646448, Length=259, Percent_Identity=33.5907335907336, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24646452, Length=259, Percent_Identity=33.5907335907336, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24646450, Length=259, Percent_Identity=33.5907335907336, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI28574284, Length=264, Percent_Identity=30.3030303030303, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI45552429, Length=264, Percent_Identity=30.3030303030303, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI62472511, Length=259, Percent_Identity=33.5907335907336, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI28571528, Length=263, Percent_Identity=33.0798479087452, Blast_Score=106, Evalue=3e-23,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR006236
- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR015508
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C; PF01842 ACT [H]

EC number: =1.1.1.95 [H]

Molecular weight: Translated: 55952; Mature: 55821

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKVLISDKLSPAAVQIFKDRGIETDVKTGLAPDELKAIIGQYDGLAIRSNTKVTTEILA
CCCEEECCCCCHHHHHHHHHCCCCCHHHCCCCHHHHHHHHCCCCCEEEECCCCHHHHHHH
AATNLKVVGRAGIGVDNVDVPAATARGIVVMNTPFGNSITTAEHAIAMMFALAREIPEAN
HHCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCC
ASTHAGKWEKNRFMGVELTGKVLGIVGCGNIGAIVADRAQGLRMRVIAYDPFLSVERAKE
CCCCCCCCCCCCEEEEEECCEEEEEEECCCCCHHHHHCCCCCEEEEEEECCCCCHHHHHH
LNVEKVELDELFPRADFITLHTPLTDATRNIIDAKAMAKMKKGVRIINCARGGLVVEEDL
CCCEEEEHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEHHHH
LAALESGQVAGAALDVFKTEPAKENALFGNPKVVCTPHLGASTSEAQENVALQVAEQMAD
HHHHHCCCCCHHEEEEHCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHH
YLLTGAVTNALNIPSVSAEDAPKLRPYMTLANQIGSFAGQLTETGIKDVKIEYLGHVASL
HHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCC
NTKPLTAMVLEGLLKPMNEAVNMVNAPVVAKERNIKVSEVKSESEGDYHTLVRLTVTTDK
CCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEECCCCCCCCEEEEEEEEEECCC
RERSVAGTLFGGNKARVVEIKGISIEAELGQNMLYVTNQDKPGFIGALGSLLGANGVNIA
CCCCEEEEEECCCCEEEEEEECEEEECCCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEE
TFHLGRSEAGGDAILLTQVDQPVSNDLLEKVRALPQVVQAKFLTFA
EEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PKVLISDKLSPAAVQIFKDRGIETDVKTGLAPDELKAIIGQYDGLAIRSNTKVTTEILA
CCEEECCCCCHHHHHHHHHCCCCCHHHCCCCHHHHHHHHCCCCCEEEECCCCHHHHHHH
AATNLKVVGRAGIGVDNVDVPAATARGIVVMNTPFGNSITTAEHAIAMMFALAREIPEAN
HHCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCC
ASTHAGKWEKNRFMGVELTGKVLGIVGCGNIGAIVADRAQGLRMRVIAYDPFLSVERAKE
CCCCCCCCCCCCEEEEEECCEEEEEEECCCCCHHHHHCCCCCEEEEEEECCCCCHHHHHH
LNVEKVELDELFPRADFITLHTPLTDATRNIIDAKAMAKMKKGVRIINCARGGLVVEEDL
CCCEEEEHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEHHHH
LAALESGQVAGAALDVFKTEPAKENALFGNPKVVCTPHLGASTSEAQENVALQVAEQMAD
HHHHHCCCCCHHEEEEHCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHH
YLLTGAVTNALNIPSVSAEDAPKLRPYMTLANQIGSFAGQLTETGIKDVKIEYLGHVASL
HHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCC
NTKPLTAMVLEGLLKPMNEAVNMVNAPVVAKERNIKVSEVKSESEGDYHTLVRLTVTTDK
CCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEECCCCCCCCEEEEEEEEEECCC
RERSVAGTLFGGNKARVVEIKGISIEAELGQNMLYVTNQDKPGFIGALGSLLGANGVNIA
CCCCEEEEEECCCCEEEEEEECEEEECCCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEE
TFHLGRSEAGGDAILLTQVDQPVSNDLLEKVRALPQVVQAKFLTFA
EEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]