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The map label for this gene is ppx [C]

Identifier: 83311938

GI number: 83311938

Start: 3037034

End: 3038518

Strand: Reverse

Name: ppx [C]

Synonym: amb2839

Alternate gene names: 83311938

Gene position: 3038518-3037034 (Counterclockwise)

Preceding gene: 83311939

Following gene: 83311937

Centisome position: 61.17

GC content: 67.68

Gene sequence:

>1485_bases
ATGAAGATCAAGCTGCGCCAGGAGCCCATCGGCATCGTCGATATCGGCTCCAACTCCATTCGCCTGTGCGTCTACGATCT
GGCGCAGCGCGTGCCCGTTCCGCTGTTCAACGAGAAGGCGGTGTGCGGCCTGGGCCAGGGCGTGGGCAGCTCCGGCCGCC
TCAGCCCCGAAGGCGTGGAGGCCGCCCTGGTGGCGGTGGGCCGCTTCATCAGCCTGTGCCGCGCCATGGAGGTGGAGCGC
CTGGACGTGCTGGCCACGGCGGCGGTGCGCGATGCCGCCGACGGCGAGGATTTCGTGCATGAGGTGGAGCGGCGCTTCGC
CGTTCAGGTCAAGGTGCTGTCCGGCGGGCAGGAAGCCAAGATGGCCGCCATGGGCGTGCTGTGCGGCACCCCCGACGCCA
ACGGCATCGTCGCCGACTTAGGCGGCGGCTCGCTGGAACTGGTGACCGTTCAGGACCAGAATTTCGGCCTGCATGTCACC
ATGCCCCTGGGGCTGCTGCGCCTCACCGAGGCTTCGGGCGACGACAAGGCGCGGGCCGCCGAGATTGTCGACGGCCATCT
GAAGGGGATTGCCTGGCTGGCCGAGGGCCGGGGGCGCAATCTCTATGCGGTCGGCGGCGCCTGGCGCTCCATCGCGCGGA
TCTGCATCGCCCAGACCCAGCATCCGCTGACCGTGCTGGACAATTTCTCGCTGGATGCCCGCGAGGCCCTGGCCATCCTG
GAACTGGTCTCGACCCAAAGCCGCAAGTCGCTGGAGAAGATTCCCGGCATCTCCAAGAAGCGCACCAGCGGCCTGCCCAT
GGCGGCGCTGATCCTGGCACGCACCATCCGCGCCATCGGACCGTCCCGGCTGGTGTTTTCCATCTACGGCATGCGGGAGG
GTCAGTTCCTCAAGCGCCTGCCGGAAAAGCTGAAGCACGAGGACCCGCTGCTGTCGGTGTGCCGCCACATGGCGCTGCTG
AACTCGCGCTTTCCCGAGCACGGCGACGAATTGCTGGCCTGGATGGCGCCGCTGTTCCCATCGGAGACCACGCGGGAAAG
CCAGTTGCGCCACGCCGCCTGTCTGGTGTCGGACATCTTCTGGAACGAGCATCCCGATTACCGCGCCGAGCAGGCCTTCC
TGCGCATCCTGCGCCTGCCCTTCATGGGCGTCGGCCATCGTCATCGCGCCGCCATCGCCTTCGCGGTGTTCTGCCGCTAC
CAGGGCAACGAGGACTCGCCCGAGGTGGCCCGCTTCATCCAGTTGCTGGACGAAGGCGCCTTCCGCCGCGCCCGCACGGT
GGGGCTGGCGTTGCGCCTAGCCCATACCTTGTCGGGCGGCGCTCCCAACCTGCTGCGCCAGACCCGGCTGTTCGTCGAGG
ACAAATACCTGATCCTCGAAGTGCCCGCCGACAATCCGGCCTTTACCCTGGAGGGCGACCGTTCCTTCGACCGCCTCGCC
AAGGCCCTGGAATGCGAGGCCCTGACCTTCCGGCGGGTGAAGTGA

Upstream 100 bases:

>100_bases
CGCCCACACCTATTTCATGACCAACCCGTCGCTGTCGGGACGAGGAAGCGCGGGGGAACGCGGACGCAAATCCAAGAATC
TGCAACTTGCCTGACGCCCC

Downstream 100 bases:

>100_bases
CCAAAATTTATATCGACGGCGACGCCTGTCCGGTGAAGGACGAGGTGTTCAAGGTGGCTGGGCGCTATGGTCTGGCCGTC
ACCGTGGTGGGGAATGCCTG

Product: exopolyphosphatase

Products: (polyphosphate)n-1; phosphate

Alternate protein names: NA

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MKIKLRQEPIGIVDIGSNSIRLCVYDLAQRVPVPLFNEKAVCGLGQGVGSSGRLSPEGVEAALVAVGRFISLCRAMEVER
LDVLATAAVRDAADGEDFVHEVERRFAVQVKVLSGGQEAKMAAMGVLCGTPDANGIVADLGGGSLELVTVQDQNFGLHVT
MPLGLLRLTEASGDDKARAAEIVDGHLKGIAWLAEGRGRNLYAVGGAWRSIARICIAQTQHPLTVLDNFSLDAREALAIL
ELVSTQSRKSLEKIPGISKKRTSGLPMAALILARTIRAIGPSRLVFSIYGMREGQFLKRLPEKLKHEDPLLSVCRHMALL
NSRFPEHGDELLAWMAPLFPSETTRESQLRHAACLVSDIFWNEHPDYRAEQAFLRILRLPFMGVGHRHRAAIAFAVFCRY
QGNEDSPEVARFIQLLDEGAFRRARTVGLALRLAHTLSGGAPNLLRQTRLFVEDKYLILEVPADNPAFTLEGDRSFDRLA
KALECEALTFRRVK

Sequences:

>Translated_494_residues
MKIKLRQEPIGIVDIGSNSIRLCVYDLAQRVPVPLFNEKAVCGLGQGVGSSGRLSPEGVEAALVAVGRFISLCRAMEVER
LDVLATAAVRDAADGEDFVHEVERRFAVQVKVLSGGQEAKMAAMGVLCGTPDANGIVADLGGGSLELVTVQDQNFGLHVT
MPLGLLRLTEASGDDKARAAEIVDGHLKGIAWLAEGRGRNLYAVGGAWRSIARICIAQTQHPLTVLDNFSLDAREALAIL
ELVSTQSRKSLEKIPGISKKRTSGLPMAALILARTIRAIGPSRLVFSIYGMREGQFLKRLPEKLKHEDPLLSVCRHMALL
NSRFPEHGDELLAWMAPLFPSETTRESQLRHAACLVSDIFWNEHPDYRAEQAFLRILRLPFMGVGHRHRAAIAFAVFCRY
QGNEDSPEVARFIQLLDEGAFRRARTVGLALRLAHTLSGGAPNLLRQTRLFVEDKYLILEVPADNPAFTLEGDRSFDRLA
KALECEALTFRRVK
>Mature_494_residues
MKIKLRQEPIGIVDIGSNSIRLCVYDLAQRVPVPLFNEKAVCGLGQGVGSSGRLSPEGVEAALVAVGRFISLCRAMEVER
LDVLATAAVRDAADGEDFVHEVERRFAVQVKVLSGGQEAKMAAMGVLCGTPDANGIVADLGGGSLELVTVQDQNFGLHVT
MPLGLLRLTEASGDDKARAAEIVDGHLKGIAWLAEGRGRNLYAVGGAWRSIARICIAQTQHPLTVLDNFSLDAREALAIL
ELVSTQSRKSLEKIPGISKKRTSGLPMAALILARTIRAIGPSRLVFSIYGMREGQFLKRLPEKLKHEDPLLSVCRHMALL
NSRFPEHGDELLAWMAPLFPSETTRESQLRHAACLVSDIFWNEHPDYRAEQAFLRILRLPFMGVGHRHRAAIAFAVFCRY
QGNEDSPEVARFIQLLDEGAFRRARTVGLALRLAHTLSGGAPNLLRQTRLFVEDKYLILEVPADNPAFTLEGDRSFDRLA
KALECEALTFRRVK

Specific function: Degradation Of Inorganic Polyphosphates. Orthophosphate Is Released Progressively From The Ends Of Polyphosphate Of Circa 500 Residues Long, While Chains Of Circa 15 Residues Compete Poorly With Polyphosphate As Substrate. [C]

COG id: COG0248

COG function: function code FP; Exopolyphosphatase

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gppA/ppx family [H]

Homologues:

Organism=Escherichia coli, GI1788848, Length=312, Percent_Identity=21.7948717948718, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321185, Length=459, Percent_Identity=25.2723311546841, Blast_Score=89, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003695 [H]

Pfam domain/function: PF02541 Ppx-GppA [H]

EC number: 3.6.1.11

Molecular weight: Translated: 54253; Mature: 54253

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKLRQEPIGIVDIGSNSIRLCVYDLAQRVPVPLFNEKAVCGLGQGVGSSGRLSPEGVE
CEEEEECCCCCEEEECCCCEEEHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCCCHHHHH
AALVAVGRFISLCRAMEVERLDVLATAAVRDAADGEDFVHEVERRFAVQVKVLSGGQEAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEEEEECCCCHHH
MAAMGVLCGTPDANGIVADLGGGSLELVTVQDQNFGLHVTMPLGLLRLTEASGDDKARAA
HHHHHEEECCCCCCCEEEECCCCEEEEEEEECCCCCEEEECCCCEEEEECCCCCCHHHHH
EIVDGHLKGIAWLAEGRGRNLYAVGGAWRSIARICIAQTQHPLTVLDNFSLDAREALAIL
HHHHHHHHHEEEEECCCCCEEEEECHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHH
ELVSTQSRKSLEKIPGISKKRTSGLPMAALILARTIRAIGPSRLVFSIYGMREGQFLKRL
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHH
PEKLKHEDPLLSVCRHMALLNSRFPEHGDELLAWMAPLFPSETTRESQLRHAACLVSDIF
HHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
WNEHPDYRAEQAFLRILRLPFMGVGHRHRAAIAFAVFCRYQGNEDSPEVARFIQLLDEGA
CCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHH
FRRARTVGLALRLAHTLSGGAPNLLRQTRLFVEDKYLILEVPADNPAFTLEGDRSFDRLA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEECCEEEEEECCCCCCEEEECCCCHHHHH
KALECEALTFRRVK
HHHHHHHHHHCCCC
>Mature Secondary Structure
MKIKLRQEPIGIVDIGSNSIRLCVYDLAQRVPVPLFNEKAVCGLGQGVGSSGRLSPEGVE
CEEEEECCCCCEEEECCCCEEEHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCCCHHHHH
AALVAVGRFISLCRAMEVERLDVLATAAVRDAADGEDFVHEVERRFAVQVKVLSGGQEAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEEEEEECCCCHHH
MAAMGVLCGTPDANGIVADLGGGSLELVTVQDQNFGLHVTMPLGLLRLTEASGDDKARAA
HHHHHEEECCCCCCCEEEECCCCEEEEEEEECCCCCEEEECCCCEEEEECCCCCCHHHHH
EIVDGHLKGIAWLAEGRGRNLYAVGGAWRSIARICIAQTQHPLTVLDNFSLDAREALAIL
HHHHHHHHHEEEEECCCCCEEEEECHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHH
ELVSTQSRKSLEKIPGISKKRTSGLPMAALILARTIRAIGPSRLVFSIYGMREGQFLKRL
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHH
PEKLKHEDPLLSVCRHMALLNSRFPEHGDELLAWMAPLFPSETTRESQLRHAACLVSDIF
HHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
WNEHPDYRAEQAFLRILRLPFMGVGHRHRAAIAFAVFCRYQGNEDSPEVARFIQLLDEGA
CCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHH
FRRARTVGLALRLAHTLSGGAPNLLRQTRLFVEDKYLILEVPADNPAFTLEGDRSFDRLA
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEECCEEEEEECCCCCCEEEECCCCHHHHH
KALECEALTFRRVK
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (polyphosphate)n; H2O

Specific reaction: (polyphosphate)n + H2O = (polyphosphate)n-1 + phosphate

General reaction: Phosphorous acid anhydride hydrolysis [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]