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The map label for this gene is rpoH [H]

Identifier: 83311906

GI number: 83311906

Start: 2998820

End: 2999698

Strand: Reverse

Name: rpoH [H]

Synonym: amb2807

Alternate gene names: 83311906

Gene position: 2999698-2998820 (Counterclockwise)

Preceding gene: 83311907

Following gene: 83311901

Centisome position: 60.39

GC content: 64.62

Gene sequence:

>879_bases
ATGTCGGTAGCCAATCTTCCGGTCGTCGCAGGTGATGACGGCCTTGCCAAATATCTCAGGGATATCAAGGAGTTTCCTGT
TCTCGCTCCCGAGGAGGAATTCATGCTGGCCAAGCGCTGGGTGGATTACGAGGACACGGATGCCGCCCATCGGCTGGTGA
CCAGCCATCTGCGCCTGGTCGCCAAGATCGCGCTGGGCTACCGCCATTACGGCTTGCCCGTGGCCGACCTCATCAGCGAG
GGCAATATCGGCCTGATGCGCGCCGTCAAGAAATTCGAGCCCGATCGCGGGTTCCGTCTGGCCACCTATGCCATGTGGTG
GATCAAGGCCTCGCTCAACGAGTTCGTGCTCAACTCGTGGTCCATGGTCAAGATCGGCACCCTGGCCGCCCAGAAGAAGC
TGTTCTTCAACCTGCGCCGCATCAAGTCGCGGCTGCGGCTGCTGGAGGCCGGCGACCTCGCCCCCGAGCATGTGGCCACC
ATCGCCCGTGAACTGGACGTGGACGAGCGCGACGTGGTCGCCATGAACCGCCGCATGGCGGGGCGCGATTCCTCGCTCAA
CGCGCCGGTGGGCGAGGGCGGGACCGAGATTCAGGACCTGATGGCCGACGAGGCCGACAATCAGGAAACCGCTTATGGCA
AACGCGAGGAACTGTCCAAGGGCCGGGTTCTGATCGCCCGGGCGCTGGATTCGCTGACCGAGCGCGAGCGGGAGGTGTTC
GTCGAACGCCGTCTGCGCGACGATCCGGTCACCTTGGAAGAACTGGGCGGGCGCTACGGCGTCTCGCGCGAGCGCATCCG
CCAGATCGAGGTCAAGGCCTTCGAGAAGGTGCGCGATCTGGTCCAGGCGGGATTCCTGCCAGCACCGGCGCGGGCGTGA

Upstream 100 bases:

>100_bases
GTAACGTGAACGAGTGCTTCGACTAGCGGTCTTTTCCGGTTTTCGAATTGTTTCCAGGCGTGTTGGGCGTCAGGAAGGCA
TCAACTGGAGGGATCAGATC

Downstream 100 bases:

>100_bases
TCATTGGGGCGCTTGCCCCAAGCCCTAGCCGAGGCGATGCCCCGGACCCCCTTTGATTTCTGATCATCGGGGGCGGGGGC
CTCGGCGGTTCGCGTTTCTT

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MSVANLPVVAGDDGLAKYLRDIKEFPVLAPEEEFMLAKRWVDYEDTDAAHRLVTSHLRLVAKIALGYRHYGLPVADLISE
GNIGLMRAVKKFEPDRGFRLATYAMWWIKASLNEFVLNSWSMVKIGTLAAQKKLFFNLRRIKSRLRLLEAGDLAPEHVAT
IARELDVDERDVVAMNRRMAGRDSSLNAPVGEGGTEIQDLMADEADNQETAYGKREELSKGRVLIARALDSLTEREREVF
VERRLRDDPVTLEELGGRYGVSRERIRQIEVKAFEKVRDLVQAGFLPAPARA

Sequences:

>Translated_292_residues
MSVANLPVVAGDDGLAKYLRDIKEFPVLAPEEEFMLAKRWVDYEDTDAAHRLVTSHLRLVAKIALGYRHYGLPVADLISE
GNIGLMRAVKKFEPDRGFRLATYAMWWIKASLNEFVLNSWSMVKIGTLAAQKKLFFNLRRIKSRLRLLEAGDLAPEHVAT
IARELDVDERDVVAMNRRMAGRDSSLNAPVGEGGTEIQDLMADEADNQETAYGKREELSKGRVLIARALDSLTEREREVF
VERRLRDDPVTLEELGGRYGVSRERIRQIEVKAFEKVRDLVQAGFLPAPARA
>Mature_291_residues
SVANLPVVAGDDGLAKYLRDIKEFPVLAPEEEFMLAKRWVDYEDTDAAHRLVTSHLRLVAKIALGYRHYGLPVADLISEG
NIGLMRAVKKFEPDRGFRLATYAMWWIKASLNEFVLNSWSMVKIGTLAAQKKLFFNLRRIKSRLRLLEAGDLAPEHVATI
ARELDVDERDVVAMNRRMAGRDSSLNAPVGEGGTEIQDLMADEADNQETAYGKREELSKGRVLIARALDSLTEREREVFV
ERRLRDDPVTLEELGGRYGVSRERIRQIEVKAFEKVRDLVQAGFLPAPARA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=283, Percent_Identity=41.3427561837456, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1789098, Length=282, Percent_Identity=35.1063829787234, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1789448, Length=238, Percent_Identity=34.8739495798319, Blast_Score=104, Evalue=8e-24,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 33021; Mature: 32890

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVANLPVVAGDDGLAKYLRDIKEFPVLAPEEEFMLAKRWVDYEDTDAAHRLVTSHLRLV
CCCCCCCEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
AKIALGYRHYGLPVADLISEGNIGLMRAVKKFEPDRGFRLATYAMWWIKASLNEFVLNSW
HHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
SMVKIGTLAAQKKLFFNLRRIKSRLRLLEAGDLAPEHVATIARELDVDERDVVAMNRRMA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHC
GRDSSLNAPVGEGGTEIQDLMADEADNQETAYGKREELSKGRVLIARALDSLTEREREVF
CCCCCCCCCCCCCCHHHHHHHHCCCCCCHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHH
VERRLRDDPVTLEELGGRYGVSRERIRQIEVKAFEKVRDLVQAGFLPAPARA
HHHHHCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SVANLPVVAGDDGLAKYLRDIKEFPVLAPEEEFMLAKRWVDYEDTDAAHRLVTSHLRLV
CCCCCCEEECCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
AKIALGYRHYGLPVADLISEGNIGLMRAVKKFEPDRGFRLATYAMWWIKASLNEFVLNSW
HHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
SMVKIGTLAAQKKLFFNLRRIKSRLRLLEAGDLAPEHVATIARELDVDERDVVAMNRRMA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHC
GRDSSLNAPVGEGGTEIQDLMADEADNQETAYGKREELSKGRVLIARALDSLTEREREVF
CCCCCCCCCCCCCCHHHHHHHHCCCCCCHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHH
VERRLRDDPVTLEELGGRYGVSRERIRQIEVKAFEKVRDLVQAGFLPAPARA
HHHHHCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8606166 [H]