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The map label for this gene is tig

Identifier: 83311892

GI number: 83311892

Start: 2981934

End: 2983379

Strand: Reverse

Name: tig

Synonym: amb2793

Alternate gene names: 83311892

Gene position: 2983379-2981934 (Counterclockwise)

Preceding gene: 83311893

Following gene: 83311891

Centisome position: 60.06

GC content: 64.87

Gene sequence:

>1446_bases
ATGCCGACCGGCGGGAGCCGTGATTTCAACTCACTCGATTCGATTGGCGATAGACTGATGCAGGTAACCGAGATCAATGC
CGAAGGCCTGAAGCGCGAATTCAAGGTCGTCGTTCCCGCCCAGCAGCTGGAAACCCGGATGCAGGACAAGCTGGCGGAGA
TCGCCCGCACCGTGGCCATGCCCGGCTTCCGCCCCGGCAAGGTGCCCATGACCATCGTGCGCAAGAAGTACGGCGGCGCG
GTGATGGGCGAGATTCTGGAAAACGCCGTCAACGACGGCGCCGGCAAGGCCATCACCGATGGCGGCCTGCGTCCGGCCAT
GCAGCCCAAGATCGAGATCACCAAGTACGAGGAAAACTCGGACCTGGAGTTCACCGTGGCGGTGGAAGTGCTGCCCGAGA
TCAAGACCATGGATTTCGGCACCATCAACCTGGTGCGCGATAAGGCCGTGGTGCCGGATGCCGAGGTGGACGACGCCCTG
GCCAAGATCGCCGAGCGCAACGAGACCTCCGAGCCGGTCAAGCGCGCCTCCAAGTCCGGCGACGTGGTCGTCATCGACTT
CGTGGGCAAGGTTGACGGCGTGGCCTTCCCCGGCGGCACCGCCGAGGGCTACTCGCTGAAGCTGGGCTCCAACACCTTCA
TCCCCGGCTTCGAAGACCAACTGGTCGGCAAGAAGGCCGAGTCCGACGTGGAAGTGAACGTGACCTTCCCCGAGGGTTAC
GGCAATGAGACCCTGGCCGGCAAGCCCGCCCTGTTCGAGGTCAAGGTCAAGGAAGTGCGCGCCCCCAAGGCCGCCGCCGT
GGACGACGAACTGGCCAAGTCGGTCGGCCTCGAGAATCTGGACGCGCTCAAGACCGCCATCCGCGACGAAATCTCGCGCG
AGCTGGACGGCGTGTCGCGCATGAAGCTCAAGCGCGCCCTGCTGGATGCGCTTTCGGACGGTCATGACTTCCCGGTTCCC
CCCAGCATGCTGGAAGGCGAGTTCGAGGCCATCTGGAAGCAGGTGGAGGCCGACAAGGAAGCCGGCCGCCAGGACCCCGC
CGACGCCGCCAAGTCCGAGGACGAGCTGAAGGCCGACTATCGCGCCCTGGCCGAGCGCCGCGTGCGCCTGGGCCTGCTGC
TGGCCGATGTGGGCCGGGTCAACGAGATCAACGTGACCCAGGAAGACCTGAACCGCGGCATCATGATGGAAGCCCGCCGT
TACCCCGGCCAGGAACATCTGGTGCTGCAGTACTACCAGAAGAACCAGGAAGCCCTGGAATCGCTGCGTGCTCCCCTCTA
CGAGGAGAAGGTGGTCGATTTCATCCTGGAGCTGGCCAAGATCACCGACAAGGAAGTGTCGGTCGAGGACCTGCGCAAGG
ACCCCGACGAGGCTTCCGCCGACGGCGAGGCCGCCCCGGCCAAGCCGAAGAAGAAGGCCGCCGCCAAGAAGAAGGCGGCC
GAATAG

Upstream 100 bases:

>100_bases
TTGGTAGACACACTGGATTTAGGTTCCAGCGGCGCAAGCCATGGGGGTTCAAGTCCCTTCGCCCGCACCAGCCATCACCG
CCGGCCCTGGAGTCTGGCGT

Downstream 100 bases:

>100_bases
GTCCCGGTTTCATGTGCAAAGCCCGCGACATTCACCATATGAATGTCGCGGGCGGTCAACATCTTCGGCGTCCGCCCCCG
CCGTTGCCATTGCCAAAGGA

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MPTGGSRDFNSLDSIGDRLMQVTEINAEGLKREFKVVVPAQQLETRMQDKLAEIARTVAMPGFRPGKVPMTIVRKKYGGA
VMGEILENAVNDGAGKAITDGGLRPAMQPKIEITKYEENSDLEFTVAVEVLPEIKTMDFGTINLVRDKAVVPDAEVDDAL
AKIAERNETSEPVKRASKSGDVVVIDFVGKVDGVAFPGGTAEGYSLKLGSNTFIPGFEDQLVGKKAESDVEVNVTFPEGY
GNETLAGKPALFEVKVKEVRAPKAAAVDDELAKSVGLENLDALKTAIRDEISRELDGVSRMKLKRALLDALSDGHDFPVP
PSMLEGEFEAIWKQVEADKEAGRQDPADAAKSEDELKADYRALAERRVRLGLLLADVGRVNEINVTQEDLNRGIMMEARR
YPGQEHLVLQYYQKNQEALESLRAPLYEEKVVDFILELAKITDKEVSVEDLRKDPDEASADGEAAPAKPKKKAAAKKKAA
E

Sequences:

>Translated_481_residues
MPTGGSRDFNSLDSIGDRLMQVTEINAEGLKREFKVVVPAQQLETRMQDKLAEIARTVAMPGFRPGKVPMTIVRKKYGGA
VMGEILENAVNDGAGKAITDGGLRPAMQPKIEITKYEENSDLEFTVAVEVLPEIKTMDFGTINLVRDKAVVPDAEVDDAL
AKIAERNETSEPVKRASKSGDVVVIDFVGKVDGVAFPGGTAEGYSLKLGSNTFIPGFEDQLVGKKAESDVEVNVTFPEGY
GNETLAGKPALFEVKVKEVRAPKAAAVDDELAKSVGLENLDALKTAIRDEISRELDGVSRMKLKRALLDALSDGHDFPVP
PSMLEGEFEAIWKQVEADKEAGRQDPADAAKSEDELKADYRALAERRVRLGLLLADVGRVNEINVTQEDLNRGIMMEARR
YPGQEHLVLQYYQKNQEALESLRAPLYEEKVVDFILELAKITDKEVSVEDLRKDPDEASADGEAAPAKPKKKAAAKKKAA
E
>Mature_480_residues
PTGGSRDFNSLDSIGDRLMQVTEINAEGLKREFKVVVPAQQLETRMQDKLAEIARTVAMPGFRPGKVPMTIVRKKYGGAV
MGEILENAVNDGAGKAITDGGLRPAMQPKIEITKYEENSDLEFTVAVEVLPEIKTMDFGTINLVRDKAVVPDAEVDDALA
KIAERNETSEPVKRASKSGDVVVIDFVGKVDGVAFPGGTAEGYSLKLGSNTFIPGFEDQLVGKKAESDVEVNVTFPEGYG
NETLAGKPALFEVKVKEVRAPKAAAVDDELAKSVGLENLDALKTAIRDEISRELDGVSRMKLKRALLDALSDGHDFPVPP
SMLEGEFEAIWKQVEADKEAGRQDPADAAKSEDELKADYRALAERRVRLGLLLADVGRVNEINVTQEDLNRGIMMEARRY
PGQEHLVLQYYQKNQEALESLRAPLYEEKVVDFILELAKITDKEVSVEDLRKDPDEASADGEAAPAKPKKKAAAKKKAAE

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation [H]

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1786640, Length=434, Percent_Identity=31.5668202764977, Blast_Score=210, Evalue=2e-55,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881 [H]

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N [H]

EC number: NA

Molecular weight: Translated: 52551; Mature: 52420

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTGGSRDFNSLDSIGDRLMQVTEINAEGLKREFKVVVPAQQLETRMQDKLAEIARTVAM
CCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCHHEEEECHHHHHHHHHHHHHHHHHHHHC
PGFRPGKVPMTIVRKKYGGAVMGEILENAVNDGAGKAITDGGLRPAMQPKIEITKYEENS
CCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
DLEFTVAVEVLPEIKTMDFGTINLVRDKAVVPDAEVDDALAKIAERNETSEPVKRASKSG
CCEEEEEEEECCCHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCC
DVVVIDFVGKVDGVAFPGGTAEGYSLKLGSNTFIPGFEDQLVGKKAESDVEVNVTFPEGY
CEEEEEECCCCCCEECCCCCCCCEEEEECCCCCCCCCHHHHHCCCCCCCEEEEEECCCCC
GNETLAGKPALFEVKVKEVRAPKAAAVDDELAKSVGLENLDALKTAIRDEISRELDGVSR
CCCCCCCCCCEEEEEHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
MKLKRALLDALSDGHDFPVPPSMLEGEFEAIWKQVEADKEAGRQDPADAAKSEDELKADY
HHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHH
RALAERRVRLGLLLADVGRVNEINVTQEDLNRGIMMEARRYPGQEHLVLQYYQKNQEALE
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHCCCCCHHHHHHHHCCHHHHH
SLRAPLYEEKVVDFILELAKITDKEVSVEDLRKDPDEASADGEAAPAKPKKKAAAKKKAA
HHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCC
E
C
>Mature Secondary Structure 
PTGGSRDFNSLDSIGDRLMQVTEINAEGLKREFKVVVPAQQLETRMQDKLAEIARTVAM
CCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCHHEEEECHHHHHHHHHHHHHHHHHHHHC
PGFRPGKVPMTIVRKKYGGAVMGEILENAVNDGAGKAITDGGLRPAMQPKIEITKYEENS
CCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCC
DLEFTVAVEVLPEIKTMDFGTINLVRDKAVVPDAEVDDALAKIAERNETSEPVKRASKSG
CCEEEEEEEECCCHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCC
DVVVIDFVGKVDGVAFPGGTAEGYSLKLGSNTFIPGFEDQLVGKKAESDVEVNVTFPEGY
CEEEEEECCCCCCEECCCCCCCCEEEEECCCCCCCCCHHHHHCCCCCCCEEEEEECCCCC
GNETLAGKPALFEVKVKEVRAPKAAAVDDELAKSVGLENLDALKTAIRDEISRELDGVSR
CCCCCCCCCCEEEEEHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
MKLKRALLDALSDGHDFPVPPSMLEGEFEAIWKQVEADKEAGRQDPADAAKSEDELKADY
HHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHH
RALAERRVRLGLLLADVGRVNEINVTQEDLNRGIMMEARRYPGQEHLVLQYYQKNQEALE
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHCCCCCHHHHHHHHCCHHHHH
SLRAPLYEEKVVDFILELAKITDKEVSVEDLRKDPDEASADGEAAPAKPKKKAAAKKKAA
HHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA