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The map label for this gene is nuoB

Identifier: 83311885

GI number: 83311885

Start: 2972647

End: 2973207

Strand: Reverse

Name: nuoB

Synonym: amb2786

Alternate gene names: 83311885

Gene position: 2973207-2972647 (Counterclockwise)

Preceding gene: 83311886

Following gene: 83311884

Centisome position: 59.86

GC content: 66.31

Gene sequence:

>561_bases
GTGAACCCGTCTGCCGGCGGCATTCTGTCGCCCGACCACGCGGCAGCGCTGCCTCCTGGCCCGGCCCAGGACGCGCTGCT
GAAGGCCGTTACCACCGAGATTTCCGACAAGGGCTTCGTGCTGGCCAGTGTGGATGCGGTGGTCAACTGGGCCCGTACCG
GCTCGTTGTGGCCCATGACCTTCGGTCTGGCCTGCTGCGCCGTGGAAATGATGCACGCGGGCTGTTCGCGCTATGACATG
GACCGCTTCGGCGTGGTCTTCCGTCCCAGCCCGCGCCAGTCGGATCTGATGATCGTCGCCGGCACGCTGACCAACAAGAT
GGCTCCGGCGCTGCGCCGCGTCTACGACCAGATGGCCGAGCCGCGCTGGGTGATCTCCATGGGCTCGTGCGCCAATGGCG
GCGGCTATTATCACTATTCCTACTCGGTGGTGCGCGGTTGCGACCGCATCGTGCCGGTGGACATCTATGTGCCGGGCTGC
CCGCCCTCGGCGGAAGCGCTGATGTACGGCATCCTGCAGCTGCAGAAGAAGATCCGCCGCACCGGCTCCATCCTCCGATA
G

Upstream 100 bases:

>100_bases
GCAAGATCGGCATGTTCGGCTTCTGGTCCATGATGGTGTTCTTGGGCGTGCTGACCATCGGCTTCATCTATGAATGGCGA
AAGGGAGCCCTGGAATGGGA

Downstream 100 bases:

>100_bases
CGCCGAAGAGGGATTCTCGAAGATGACGCAAGCGCTGAAGGATCTGGGTGAGTACATCGCCCAAGCCCTGCCCCAGGACG
TGCTGGGAACCGAAGTGAAC

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MNPSAGGILSPDHAAALPPGPAQDALLKAVTTEISDKGFVLASVDAVVNWARTGSLWPMTFGLACCAVEMMHAGCSRYDM
DRFGVVFRPSPRQSDLMIVAGTLTNKMAPALRRVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGC
PPSAEALMYGILQLQKKIRRTGSILR

Sequences:

>Translated_186_residues
MNPSAGGILSPDHAAALPPGPAQDALLKAVTTEISDKGFVLASVDAVVNWARTGSLWPMTFGLACCAVEMMHAGCSRYDM
DRFGVVFRPSPRQSDLMIVAGTLTNKMAPALRRVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGC
PPSAEALMYGILQLQKKIRRTGSILR
>Mature_186_residues
MNPSAGGILSPDHAAALPPGPAQDALLKAVTTEISDKGFVLASVDAVVNWARTGSLWPMTFGLACCAVEMMHAGCSRYDM
DRFGVVFRPSPRQSDLMIVAGTLTNKMAPALRRVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGC
PPSAEALMYGILQLQKKIRRTGSILR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=165, Percent_Identity=77.5757575757576, Blast_Score=265, Evalue=2e-71,
Organism=Escherichia coli, GI1788624, Length=171, Percent_Identity=47.3684210526316, Blast_Score=172, Evalue=1e-44,
Organism=Escherichia coli, GI1789074, Length=125, Percent_Identity=37.6, Blast_Score=98, Evalue=3e-22,
Organism=Escherichia coli, GI1788834, Length=121, Percent_Identity=36.3636363636364, Blast_Score=89, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17509685, Length=146, Percent_Identity=75.3424657534247, Blast_Score=249, Evalue=6e-67,
Organism=Drosophila melanogaster, GI18859983, Length=154, Percent_Identity=75.974025974026, Blast_Score=263, Evalue=6e-71,
Organism=Drosophila melanogaster, GI24642371, Length=154, Percent_Identity=75.974025974026, Blast_Score=263, Evalue=6e-71,
Organism=Drosophila melanogaster, GI24651058, Length=146, Percent_Identity=78.7671232876712, Blast_Score=255, Evalue=1e-68,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_MAGSA (Q2W3I5)

Other databases:

- EMBL:   AP007255
- RefSeq:   YP_422149.1
- HSSP:   Q56218
- ProteinModelPortal:   Q2W3I5
- SMR:   Q2W3I5
- STRING:   Q2W3I5
- GeneID:   3803876
- GenomeReviews:   AP007255_GR
- KEGG:   mag:amb2786
- NMPDR:   fig|342108.5.peg.2473
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   GKPIGSN
- PhylomeDB:   Q2W3I5
- ProtClustDB:   PRK06411
- BioCyc:   MMAG342108:AMB2786-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 20116; Mature: 20116

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
8.6 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
8.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPSAGGILSPDHAAALPPGPAQDALLKAVTTEISDKGFVLASVDAVVNWARTGSLWPMT
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCCHHH
FGLACCAVEMMHAGCSRYDMDRFGVVFRPSPRQSDLMIVAGTLTNKMAPALRRVYDQMAE
HHHHHHHHHHHHCCCCCCCHHHCCEEECCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCC
PRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPSAEALMYGILQLQKKIRR
CEEEEEECCCCCCCCEEEEEHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
TGSILR
HCCCCC
>Mature Secondary Structure
MNPSAGGILSPDHAAALPPGPAQDALLKAVTTEISDKGFVLASVDAVVNWARTGSLWPMT
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCCHHH
FGLACCAVEMMHAGCSRYDMDRFGVVFRPSPRQSDLMIVAGTLTNKMAPALRRVYDQMAE
HHHHHHHHHHHHCCCCCCCHHHCCEEECCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCC
PRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPSAEALMYGILQLQKKIRR
CEEEEEECCCCCCCCEEEEEHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
TGSILR
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA