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The map label for this gene is 83311870

Identifier: 83311870

GI number: 83311870

Start: 2956288

End: 2957937

Strand: Reverse

Name: 83311870

Synonym: amb2771

Alternate gene names: NA

Gene position: 2957937-2956288 (Counterclockwise)

Preceding gene: 83311871

Following gene: 83311869

Centisome position: 59.55

GC content: 65.88

Gene sequence:

>1650_bases
ATGAGCCAGGGCGAACTCCTCTTCCTGCCCCTGGGGGGCGTCGGCGAGATCGGCATGAATCTCGCGCTGTACGGCTATGA
CGGCAAGTGGCTGATGGTCGATTGCGGCGTCTCCTTCGGCGACGAGACCATGCCCATGGTCGACGTGGTGATGGCCGATC
CCGCCTGGATCGAGGAACGCCGCGACAAGCTGGCGGGCATCGTCATCACCCATGGTCACGAGGACCATCTGGGCGCCGTC
CAGTATCTGTGGGACCGGCTGCGCTGTCCGGTGTGGGCGACGCCGTTCTCGGCCTCCATCCTGAAGAACAAGCTGCACGA
GACCGGGCTGCAGGCCCAGGTGCCTCTCAACGTAGTTGAGCTGGGATCGCGCTTTAAGGTCGGCCCGTTCGAGATCGAAA
TGATCTCCATCACTCATTCCATTCCCGAGCCGAATCTGCTGGCTATCCGCACGCCGCTGGGCACCGTCGTCCATACGGGC
GACTGGAAGTTCGACCCCGATCCGCTGCTGGGCCTGCCCACCGATACAGAAGCTCTGCGCCGGGTGGGCAAGGAGGGCGT
CCTGGCCCTGATCGGCGATTCCACCAATGTTTTCACCAAGGGCCATTCCGGTTCGGAATCCGAGGTGCGTGGCAGCCTGA
TCGAGCTGCTGGGCCGCTTTGACGGGCGCATCGCCGTGTCGTGCTTCGCCACCAACGTGGCGCGGCTGGAAAGCATCACC
GTGGCGGCCATGGCCAATGACCGCAGCGTCGCCCTGGTGGGCCGGAGCCTGTGGCGCATCGATAAGGCGGCGCGGGAGAA
CGGCTATCTCGCCGACCTGCCGCCCTTCCTTACCGAGCACGATGCCGGTTACCTGCCCAAGGACAAGGTGGTCTATATCT
GCACCGGCAGCCAGGGAGAGCCCCGCGCCGCCCTGGCCCGCATCGCGTCGGGCGACCATCCCCATGTGCATCTGGGCAAG
GGGGACGTCTGCATCTTCTCGTCGCGGATCATTCCCGGCAACGAGAAGGACATTTTCAAGCTTCAGAATGATTTGGTCCG
CCAGGGCGTCGAGGTGGTGACCGAGAAGGATGAATTCGTCCATGTCTCCGGCCATCCCGCCCGCCAGGAGATGGAGGAAA
TGTATCGCCTGCTGCGGCCGCGCGTCGCGGTGCCGGTGCATGGCGAGGCCCGCCACCTGCAGGAGCACGCTCGGCTGGCG
CGGGCTTGCGGGGTGGAGGAGGCTATCGAGACCTCCAACGGCGCCATGCTGCGCCTCGCTCCCGGTCCGGTGGAGGTGAT
TGACCACGTGCCCACCGGCAAGCTGTGCGTCGATGGCCCGCGCATCGTCCGTCTGGATTCCGAGATCCTGCGCAATCGCC
GTCGCATGGTGTTCAACGGCTCGGCCGTGGTTACCGTGGTGCTGGACAAGGTCGGCAAGCTGCTGGGCGATCCGCAACTG
ACGGCCCTGGGCCTGCTGGATGCCGGTCACGAGGCGGAAGAGCACGACGCGGTGGTCGAGGCCGTGCGTGACGCCATCGA
GGAACTGCCGCAGAAGGTCCGCCGGGACGACGCCGTGGTGCGCGAAGCCGCCCGGCTGGCGGTGCGTCGCTCGTTGCGTG
ACACCCACGGCAAGAAGCCCATCACCGAGGTGCATCTGGTCCGGGTCTGA

Upstream 100 bases:

>100_bases
TGTTTGCCGAGGCGACCAAGGTGATCAACGCGCTGGACGCCGACCTGACCCTGCGCGGCCTGCTGGAAATCCATCGCCGC
AATTCGACGCCGGTGCGGGG

Downstream 100 bases:

>100_bases
GTCACCAAATCGTCATGGCCCTGACACGGAAGAGTCCCGCTTGGTCCCTATGATCCCCCCTCGGGGGACGTGGGGTTCAC
AAGGCCGAGGACATGACCGT

Product: metallo-beta-lactamase superfamily hydrolase

Products: NA

Alternate protein names: RNase J [H]

Number of amino acids: Translated: 549; Mature: 548

Protein sequence:

>549_residues
MSQGELLFLPLGGVGEIGMNLALYGYDGKWLMVDCGVSFGDETMPMVDVVMADPAWIEERRDKLAGIVITHGHEDHLGAV
QYLWDRLRCPVWATPFSASILKNKLHETGLQAQVPLNVVELGSRFKVGPFEIEMISITHSIPEPNLLAIRTPLGTVVHTG
DWKFDPDPLLGLPTDTEALRRVGKEGVLALIGDSTNVFTKGHSGSESEVRGSLIELLGRFDGRIAVSCFATNVARLESIT
VAAMANDRSVALVGRSLWRIDKAARENGYLADLPPFLTEHDAGYLPKDKVVYICTGSQGEPRAALARIASGDHPHVHLGK
GDVCIFSSRIIPGNEKDIFKLQNDLVRQGVEVVTEKDEFVHVSGHPARQEMEEMYRLLRPRVAVPVHGEARHLQEHARLA
RACGVEEAIETSNGAMLRLAPGPVEVIDHVPTGKLCVDGPRIVRLDSEILRNRRRMVFNGSAVVTVVLDKVGKLLGDPQL
TALGLLDAGHEAEEHDAVVEAVRDAIEELPQKVRRDDAVVREAARLAVRRSLRDTHGKKPITEVHLVRV

Sequences:

>Translated_549_residues
MSQGELLFLPLGGVGEIGMNLALYGYDGKWLMVDCGVSFGDETMPMVDVVMADPAWIEERRDKLAGIVITHGHEDHLGAV
QYLWDRLRCPVWATPFSASILKNKLHETGLQAQVPLNVVELGSRFKVGPFEIEMISITHSIPEPNLLAIRTPLGTVVHTG
DWKFDPDPLLGLPTDTEALRRVGKEGVLALIGDSTNVFTKGHSGSESEVRGSLIELLGRFDGRIAVSCFATNVARLESIT
VAAMANDRSVALVGRSLWRIDKAARENGYLADLPPFLTEHDAGYLPKDKVVYICTGSQGEPRAALARIASGDHPHVHLGK
GDVCIFSSRIIPGNEKDIFKLQNDLVRQGVEVVTEKDEFVHVSGHPARQEMEEMYRLLRPRVAVPVHGEARHLQEHARLA
RACGVEEAIETSNGAMLRLAPGPVEVIDHVPTGKLCVDGPRIVRLDSEILRNRRRMVFNGSAVVTVVLDKVGKLLGDPQL
TALGLLDAGHEAEEHDAVVEAVRDAIEELPQKVRRDDAVVREAARLAVRRSLRDTHGKKPITEVHLVRV
>Mature_548_residues
SQGELLFLPLGGVGEIGMNLALYGYDGKWLMVDCGVSFGDETMPMVDVVMADPAWIEERRDKLAGIVITHGHEDHLGAVQ
YLWDRLRCPVWATPFSASILKNKLHETGLQAQVPLNVVELGSRFKVGPFEIEMISITHSIPEPNLLAIRTPLGTVVHTGD
WKFDPDPLLGLPTDTEALRRVGKEGVLALIGDSTNVFTKGHSGSESEVRGSLIELLGRFDGRIAVSCFATNVARLESITV
AAMANDRSVALVGRSLWRIDKAARENGYLADLPPFLTEHDAGYLPKDKVVYICTGSQGEPRAALARIASGDHPHVHLGKG
DVCIFSSRIIPGNEKDIFKLQNDLVRQGVEVVTEKDEFVHVSGHPARQEMEEMYRLLRPRVAVPVHGEARHLQEHARLAR
ACGVEEAIETSNGAMLRLAPGPVEVIDHVPTGKLCVDGPRIVRLDSEILRNRRRMVFNGSAVVTVVLDKVGKLLGDPQLT
ALGLLDAGHEAEEHDAVVEAVRDAIEELPQKVRRDDAVVREAARLAVRRSLRDTHGKKPITEVHLVRV

Specific function: Unknown

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 60231; Mature: 60100

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQGELLFLPLGGVGEIGMNLALYGYDGKWLMVDCGVSFGDETMPMVDVVMADPAWIEER
CCCCCEEEEECCCCCCCCCEEEEEEECCEEEEEECCCCCCCCCCCCEEEEECCCHHHHHH
RDKLAGIVITHGHEDHLGAVQYLWDRLRCPVWATPFSASILKNKLHETGLQAQVPLNVVE
HHHHEEEEEECCCCCHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHCCCCEECCCHHHH
LGSRFKVGPFEIEMISITHSIPEPNLLAIRTPLGTVVHTGDWKFDPDPLLGLPTDTEALR
CCCCCCCCCEEEEEEEEEECCCCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCCHHHHH
RVGKEGVLALIGDSTNVFTKGHSGSESEVRGSLIELLGRFDGRIAVSCFATNVARLESIT
HCCCCCEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHEE
VAAMANDRSVALVGRSLWRIDKAARENGYLADLPPFLTEHDAGYLPKDKVVYICTGSQGE
EEEECCCCEEEEHHHHHHHHHHHHHCCCCEECCCCHHHCCCCCCCCCCCEEEEEECCCCC
PRAALARIASGDHPHVHLGKGDVCIFSSRIIPGNEKDIFKLQNDLVRQGVEVVTEKDEFV
HHHHHHHHHCCCCCEEEECCCCEEEEECEECCCCCHHHHHHHHHHHHHHHHHEECCCCEE
HVSGHPARQEMEEMYRLLRPRVAVPVHGEARHLQEHARLARACGVEEAIETSNGAMLRLA
EECCCCHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEC
PGPVEVIDHVPTGKLCVDGPRIVRLDSEILRNRRRMVFNGSAVVTVVLDKVGKLLGDPQL
CCCHHHHCCCCCCCEEECCCEEEEECHHHHHCCCCEEECCCEEEEHHHHHHHHHHCCCCE
TALGLLDAGHEAEEHDAVVEAVRDAIEELPQKVRRDDAVVREAARLAVRRSLRDTHGKKP
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ITEVHLVRV
CCCEEEEEC
>Mature Secondary Structure 
SQGELLFLPLGGVGEIGMNLALYGYDGKWLMVDCGVSFGDETMPMVDVVMADPAWIEER
CCCCEEEEECCCCCCCCCEEEEEEECCEEEEEECCCCCCCCCCCCEEEEECCCHHHHHH
RDKLAGIVITHGHEDHLGAVQYLWDRLRCPVWATPFSASILKNKLHETGLQAQVPLNVVE
HHHHEEEEEECCCCCHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHCCCCEECCCHHHH
LGSRFKVGPFEIEMISITHSIPEPNLLAIRTPLGTVVHTGDWKFDPDPLLGLPTDTEALR
CCCCCCCCCEEEEEEEEEECCCCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCCHHHHH
RVGKEGVLALIGDSTNVFTKGHSGSESEVRGSLIELLGRFDGRIAVSCFATNVARLESIT
HCCCCCEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHEE
VAAMANDRSVALVGRSLWRIDKAARENGYLADLPPFLTEHDAGYLPKDKVVYICTGSQGE
EEEECCCCEEEEHHHHHHHHHHHHHCCCCEECCCCHHHCCCCCCCCCCCEEEEEECCCCC
PRAALARIASGDHPHVHLGKGDVCIFSSRIIPGNEKDIFKLQNDLVRQGVEVVTEKDEFV
HHHHHHHHHCCCCCEEEECCCCEEEEECEECCCCCHHHHHHHHHHHHHHHHHEECCCCEE
HVSGHPARQEMEEMYRLLRPRVAVPVHGEARHLQEHARLARACGVEEAIETSNGAMLRLA
EECCCCHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEC
PGPVEVIDHVPTGKLCVDGPRIVRLDSEILRNRRRMVFNGSAVVTVVLDKVGKLLGDPQL
CCCHHHHCCCCCCCEEECCCEEEEECHHHHHCCCCEEECCCEEEEHHHHHHHHHHCCCCE
TALGLLDAGHEAEEHDAVVEAVRDAIEELPQKVRRDDAVVREAARLAVRRSLRDTHGKKP
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ITEVHLVRV
CCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]