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The map label for this gene is napA [H]

Identifier: 83311789

GI number: 83311789

Start: 2881308

End: 2883791

Strand: Reverse

Name: napA [H]

Synonym: amb2690

Alternate gene names: 83311789

Gene position: 2883791-2881308 (Counterclockwise)

Preceding gene: 83311790

Following gene: 83311788

Centisome position: 58.06

GC content: 63.49

Gene sequence:

>2484_bases
ATGAGCCTCACCAGGCGCGACTTCATCAAGGCGAACGCCGTCGCGGCCACTGCTGCCGCTGCGGGGATCGCCACGCCGGC
CATCGCCCAGCCGGCAAAAGCCAATATCCGCTGGGACAAGGGTGTCTGCCGATTCTGTGGCACCGGCTGCGCCGTGCTGG
TGGGCGTTCAGGACGGCCGTGTCGTGGCCACCCAGGGCGACCCCGACTCGCCGGTCAACCGCGGCCTCAACTGCATCAAG
GGCTACTTCCTGTCCAAGATCATGTACGGCGAGGACCGGCTGACCAAGCCCCTGCTGCGCATGAAGAACGGCAAGTTCGA
CAAGAACGGCGAATTCCAGCCGATCTCGTGGGACCAGGCCTTCGACATCATGGCGGAGAAGTGGAAGGAACAGCTGAAGA
AGCCCGACGGCGTGACCCGCGTCGGCATGTTCGGCTCGGGCCAGTGGACGATTTGGGAAGGCTATGCCGCCTCCAAGCTG
TTCAAGGCCGGCTTCCGCTCCAACAACCTGGACCCCAATGCCCGGCATTGCATGGCGTCGGCGGTGGCCGGCTTCATGCG
CACCTTCGGCATCGACGAGCCCATGGGCTGCTACGACGACATCGAGCAGACCGACGCCTTCGTGCTGTGGGGCTCCAACA
TGGCGGAGATGCACCCCATCTTGTGGTCGCGCGTCACCGATCGCCGCCTGACCCACGAGGGCTGCAAGGTCGCCGTGCTG
TCCACCTTCGAGCACCGCTCGTTCGAGCTGGCCGACATCCCCATGGTGTTCACGCCCCAGACCGATCTGGCGATCCTCAA
CTACATCTGCCACTACATCATCTCGAAGAACGCCTATGACAAGGACTTCATCGAGAAGCACGTGAACTTCAAGAAGGGGG
CCACGGATATCGGCTACGGCCTGCGTCCGACCCATGCCCTCGAGAAGGATCAGGCCAACGCCGCGACGCCGGACAAGTCC
GATCCCATGACCTTCGACGAGTTCAAGGCCTTCGTCGCCGAGTACACCCTGGAGAAGGTCGCCAAGTTGTCCGGCGTCCC
CGCCGACAAGCTGGAGGCCCTGGCCAAGCTGTACGCCGATCCCAAGGTCAAGGTGGTGTCCTTCTGGACCATGGGCTTCA
ATCAGCATGCGCGCGGCACGTGGGTCAATAACATGATCTACAATGTGCATTTGCTGATGGGCAAGATCTCCGAGCCGGGC
AACAGCCCGTTCTCGCTGACCGGCCAGCCCTCGGCCTGCGGCACGGCGCGCGAGGTCGGCACCTTTGCCCATCGCCTGCC
CGCCGACATGGTGGTGATGAACGACAAGCATCGCGAGATCACCGAAGGCCTGTGGAAGCTGCCCCCCGGCACCCTCAATC
CCAAGATTGGCTACCACGCCGTGCTGCAGCACCGCATGCTGAAGGACGGCAAGCTCAACGCCTATTGGGTGATGTGCACC
AACAACATGCAGACCGCGCCCAACATGAACGAGGAGGGGTATCCGGGCTACCGCAACCCCGCCAACTTCATCGTGGTGTC
CGACCCCTATCCCACGGTCACCGCCCTGGCCGCCGACCTGATCCTGCCCACCGCCATGTGGATGGAAAAGGAAGGCGCCT
ATGGCAATGCCGAACGCCGGACCCAGTTCTGGCGCCAGCAGGTCAAGGCTCCGGGCGAGGCCAAGTCCGACCTTTGGCAG
ATCATGGAGTTCTCCAAGCGCTTCAAGATCGAGGAAGTGTGGCCCGAGGAACTGCTTGCCAAGAAGCCCGAACTGCGCGG
CAAGACCCTGTTCGACGTGCTCTACAAGAACGGCCAGGTGGACAAGTTCCCCGCCACCGATATCCAGGCCGGATTCGAGA
ACGACGAGGCCAAGGCTTTCGGCTTCTATCCGCAGAAGGGCCTGTTCGAGGAATACGCCTCCTTCGGCCGTGGGCATGCC
CATGACCTGGCGCCGTTCGAGGCTTATCACAAGGCCCGCGGCCTCCGCTGGCCCGTGGTCGACGGCAAGGAGACCCTGTG
GCGCTTCCGCGAGGGCTATGATCCCTACGTGAAGGCGGGCGAGGGCGTGAAGTTCTATGGCAAGCCCGACGGCAAGGCCT
GGATATTCGCCCTGCCGTACCAGCCCGCCGCCGAATCGCCGGACAAGGAATTCGACCTGTGGCTGTCCACCGGCCGTGTT
CTCGAACACTGGCATTCGGGCTCCATGACCCGGCGTGTGCCCGAGTTGCATAAGTCGGTGCCCAACGCCGTGCTCTACAT
GCACCCCAACGACGCCGCCAAGCGCAACCTGCGCAACGGCGACGTGGTCAAGGTGGCGAGCCGGCGCGGCGAGGTGACCA
CCCGCATCGATACCCGTGGCCGTAACAAGCCGCCGGAGGGGCTGGTGTTCATGCCGTTCTTCGACGAAAGCCAGTTGGTC
AACAAGCTGACCCTCGACGCCACCTGCCCGATCTCCAAGGAGACGGACTACAAGAAGTGCGCCGTCAAGGTCAGCAAGGC
CTAG

Upstream 100 bases:

>100_bases
CCACGATAACCAGTCTGACCGACATCCAGGGCGTTTTGAATGTGTCCCTGGTCTATCACCACTTTGATTCCGATCTTGAT
CTGGAAGGGGAGACCGTTTC

Downstream 100 bases:

>100_bases
GAAAGAGGGTCGGGGCCGGTCCCTGACGGACGGCCCCGACCATCGGAGAACCAGACATCATGGCCAGGCACCACAAGACC
AGTGGAGATGCTGTTGACCA

Product: nitrate reductase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 827; Mature: 826

Protein sequence:

>827_residues
MSLTRRDFIKANAVAATAAAAGIATPAIAQPAKANIRWDKGVCRFCGTGCAVLVGVQDGRVVATQGDPDSPVNRGLNCIK
GYFLSKIMYGEDRLTKPLLRMKNGKFDKNGEFQPISWDQAFDIMAEKWKEQLKKPDGVTRVGMFGSGQWTIWEGYAASKL
FKAGFRSNNLDPNARHCMASAVAGFMRTFGIDEPMGCYDDIEQTDAFVLWGSNMAEMHPILWSRVTDRRLTHEGCKVAVL
STFEHRSFELADIPMVFTPQTDLAILNYICHYIISKNAYDKDFIEKHVNFKKGATDIGYGLRPTHALEKDQANAATPDKS
DPMTFDEFKAFVAEYTLEKVAKLSGVPADKLEALAKLYADPKVKVVSFWTMGFNQHARGTWVNNMIYNVHLLMGKISEPG
NSPFSLTGQPSACGTAREVGTFAHRLPADMVVMNDKHREITEGLWKLPPGTLNPKIGYHAVLQHRMLKDGKLNAYWVMCT
NNMQTAPNMNEEGYPGYRNPANFIVVSDPYPTVTALAADLILPTAMWMEKEGAYGNAERRTQFWRQQVKAPGEAKSDLWQ
IMEFSKRFKIEEVWPEELLAKKPELRGKTLFDVLYKNGQVDKFPATDIQAGFENDEAKAFGFYPQKGLFEEYASFGRGHA
HDLAPFEAYHKARGLRWPVVDGKETLWRFREGYDPYVKAGEGVKFYGKPDGKAWIFALPYQPAAESPDKEFDLWLSTGRV
LEHWHSGSMTRRVPELHKSVPNAVLYMHPNDAAKRNLRNGDVVKVASRRGEVTTRIDTRGRNKPPEGLVFMPFFDESQLV
NKLTLDATCPISKETDYKKCAVKVSKA

Sequences:

>Translated_827_residues
MSLTRRDFIKANAVAATAAAAGIATPAIAQPAKANIRWDKGVCRFCGTGCAVLVGVQDGRVVATQGDPDSPVNRGLNCIK
GYFLSKIMYGEDRLTKPLLRMKNGKFDKNGEFQPISWDQAFDIMAEKWKEQLKKPDGVTRVGMFGSGQWTIWEGYAASKL
FKAGFRSNNLDPNARHCMASAVAGFMRTFGIDEPMGCYDDIEQTDAFVLWGSNMAEMHPILWSRVTDRRLTHEGCKVAVL
STFEHRSFELADIPMVFTPQTDLAILNYICHYIISKNAYDKDFIEKHVNFKKGATDIGYGLRPTHALEKDQANAATPDKS
DPMTFDEFKAFVAEYTLEKVAKLSGVPADKLEALAKLYADPKVKVVSFWTMGFNQHARGTWVNNMIYNVHLLMGKISEPG
NSPFSLTGQPSACGTAREVGTFAHRLPADMVVMNDKHREITEGLWKLPPGTLNPKIGYHAVLQHRMLKDGKLNAYWVMCT
NNMQTAPNMNEEGYPGYRNPANFIVVSDPYPTVTALAADLILPTAMWMEKEGAYGNAERRTQFWRQQVKAPGEAKSDLWQ
IMEFSKRFKIEEVWPEELLAKKPELRGKTLFDVLYKNGQVDKFPATDIQAGFENDEAKAFGFYPQKGLFEEYASFGRGHA
HDLAPFEAYHKARGLRWPVVDGKETLWRFREGYDPYVKAGEGVKFYGKPDGKAWIFALPYQPAAESPDKEFDLWLSTGRV
LEHWHSGSMTRRVPELHKSVPNAVLYMHPNDAAKRNLRNGDVVKVASRRGEVTTRIDTRGRNKPPEGLVFMPFFDESQLV
NKLTLDATCPISKETDYKKCAVKVSKA
>Mature_826_residues
SLTRRDFIKANAVAATAAAAGIATPAIAQPAKANIRWDKGVCRFCGTGCAVLVGVQDGRVVATQGDPDSPVNRGLNCIKG
YFLSKIMYGEDRLTKPLLRMKNGKFDKNGEFQPISWDQAFDIMAEKWKEQLKKPDGVTRVGMFGSGQWTIWEGYAASKLF
KAGFRSNNLDPNARHCMASAVAGFMRTFGIDEPMGCYDDIEQTDAFVLWGSNMAEMHPILWSRVTDRRLTHEGCKVAVLS
TFEHRSFELADIPMVFTPQTDLAILNYICHYIISKNAYDKDFIEKHVNFKKGATDIGYGLRPTHALEKDQANAATPDKSD
PMTFDEFKAFVAEYTLEKVAKLSGVPADKLEALAKLYADPKVKVVSFWTMGFNQHARGTWVNNMIYNVHLLMGKISEPGN
SPFSLTGQPSACGTAREVGTFAHRLPADMVVMNDKHREITEGLWKLPPGTLNPKIGYHAVLQHRMLKDGKLNAYWVMCTN
NMQTAPNMNEEGYPGYRNPANFIVVSDPYPTVTALAADLILPTAMWMEKEGAYGNAERRTQFWRQQVKAPGEAKSDLWQI
MEFSKRFKIEEVWPEELLAKKPELRGKTLFDVLYKNGQVDKFPATDIQAGFENDEAKAFGFYPQKGLFEEYASFGRGHAH
DLAPFEAYHKARGLRWPVVDGKETLWRFREGYDPYVKAGEGVKFYGKPDGKAWIFALPYQPAAESPDKEFDLWLSTGRVL
EHWHSGSMTRRVPELHKSVPNAVLYMHPNDAAKRNLRNGDVVKVASRRGEVTTRIDTRGRNKPPEGLVFMPFFDESQLVN
KLTLDATCPISKETDYKKCAVKVSKA

Specific function: Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and peripla

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788534, Length=830, Percent_Identity=73.855421686747, Blast_Score=1301, Evalue=0.0,
Organism=Escherichia coli, GI3868721, Length=564, Percent_Identity=26.5957446808511, Blast_Score=184, Evalue=2e-47,
Organism=Escherichia coli, GI3868720, Length=660, Percent_Identity=24.6969696969697, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI3868719, Length=661, Percent_Identity=23.6006051437216, Blast_Score=122, Evalue=1e-28,
Organism=Escherichia coli, GI1787870, Length=906, Percent_Identity=21.523178807947, Blast_Score=81, Evalue=3e-16,
Organism=Escherichia coli, GI1787778, Length=343, Percent_Identity=24.198250728863, Blast_Score=74, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR010051
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 92940; Mature: 92809

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTRRDFIKANAVAATAAAAGIATPAIAQPAKANIRWDKGVCRFCGTGCAVLVGVQDGR
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHCCCEEEEEEECCCE
VVATQGDPDSPVNRGLNCIKGYFLSKIMYGEDRLTKPLLRMKNGKFDKNGEFQPISWDQA
EEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
FDIMAEKWKEQLKKPDGVTRVGMFGSGQWTIWEGYAASKLFKAGFRSNNLDPNARHCMAS
HHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCHHHHHHHHHCCCCCCCCCHHHHHHHH
AVAGFMRTFGIDEPMGCYDDIEQTDAFVLWGSNMAEMHPILWSRVTDRRLTHEGCKVAVL
HHHHHHHHCCCCCCCCCHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEE
STFEHRSFELADIPMVFTPQTDLAILNYICHYIISKNAYDKDFIEKHVNFKKGATDIGYG
ECCCCCCEEEECCCEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCC
LRPTHALEKDQANAATPDKSDPMTFDEFKAFVAEYTLEKVAKLSGVPADKLEALAKLYAD
CCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
PKVKVVSFWTMGFNQHARGTWVNNMIYNVHLLMGKISEPGNSPFSLTGQPSACGTAREVG
CCEEEEEEEEECCCCCCCCCCHHHHHEEEEEEEEECCCCCCCCEEECCCCCCCCCHHHHH
TFAHRLPADMVVMNDKHREITEGLWKLPPGTLNPKIGYHAVLQHRMLKDGKLNAYWVMCT
HHHHHCCCCEEEECCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEE
NNMQTAPNMNEEGYPGYRNPANFIVVSDPYPTVTALAADLILPTAMWMEKEGAYGNAERR
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
TQFWRQQVKAPGEAKSDLWQIMEFSKRFKIEEVWPEELLAKKPELRGKTLFDVLYKNGQV
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHCCHHHHHHCCCCCCCCHHHHHHHCCCCC
DKFPATDIQAGFENDEAKAFGFYPQKGLFEEYASFGRGHAHDLAPFEAYHKARGLRWPVV
CCCCCCHHHCCCCCCCCCEECCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCC
DGKETLWRFREGYDPYVKAGEGVKFYGKPDGKAWIFALPYQPAAESPDKEFDLWLSTGRV
CCHHHHHHHHCCCCHHHCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCEEEHHHHH
LEHWHSGSMTRRVPELHKSVPNAVLYMHPNDAAKRNLRNGDVVKVASRRGEVTTRIDTRG
HHHHCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHCCCCCCEEEEECCCCCEEEEEECCC
RNKPPEGLVFMPFFDESQLVNKLTLDATCPISKETDYKKCAVKVSKA
CCCCCCCEEEEEECCHHHHHHHHCCCCCCCCCCCCCCHHHEEEECCC
>Mature Secondary Structure 
SLTRRDFIKANAVAATAAAAGIATPAIAQPAKANIRWDKGVCRFCGTGCAVLVGVQDGR
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHCCCEEEEEEECCCE
VVATQGDPDSPVNRGLNCIKGYFLSKIMYGEDRLTKPLLRMKNGKFDKNGEFQPISWDQA
EEEECCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHH
FDIMAEKWKEQLKKPDGVTRVGMFGSGQWTIWEGYAASKLFKAGFRSNNLDPNARHCMAS
HHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCHHHHHHHHHCCCCCCCCCHHHHHHHH
AVAGFMRTFGIDEPMGCYDDIEQTDAFVLWGSNMAEMHPILWSRVTDRRLTHEGCKVAVL
HHHHHHHHCCCCCCCCCHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEE
STFEHRSFELADIPMVFTPQTDLAILNYICHYIISKNAYDKDFIEKHVNFKKGATDIGYG
ECCCCCCEEEECCCEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCC
LRPTHALEKDQANAATPDKSDPMTFDEFKAFVAEYTLEKVAKLSGVPADKLEALAKLYAD
CCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
PKVKVVSFWTMGFNQHARGTWVNNMIYNVHLLMGKISEPGNSPFSLTGQPSACGTAREVG
CCEEEEEEEEECCCCCCCCCCHHHHHEEEEEEEEECCCCCCCCEEECCCCCCCCCHHHHH
TFAHRLPADMVVMNDKHREITEGLWKLPPGTLNPKIGYHAVLQHRMLKDGKLNAYWVMCT
HHHHHCCCCEEEECCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEE
NNMQTAPNMNEEGYPGYRNPANFIVVSDPYPTVTALAADLILPTAMWMEKEGAYGNAERR
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
TQFWRQQVKAPGEAKSDLWQIMEFSKRFKIEEVWPEELLAKKPELRGKTLFDVLYKNGQV
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHCCHHHHHHCCCCCCCCHHHHHHHCCCCC
DKFPATDIQAGFENDEAKAFGFYPQKGLFEEYASFGRGHAHDLAPFEAYHKARGLRWPVV
CCCCCCHHHCCCCCCCCCEECCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCC
DGKETLWRFREGYDPYVKAGEGVKFYGKPDGKAWIFALPYQPAAESPDKEFDLWLSTGRV
CCHHHHHHHHCCCCHHHCCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCEEEHHHHH
LEHWHSGSMTRRVPELHKSVPNAVLYMHPNDAAKRNLRNGDVVKVASRRGEVTTRIDTRG
HHHHCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHCCCCCCEEEEECCCCCEEEEEECCC
RNKPPEGLVFMPFFDESQLVNKLTLDATCPISKETDYKKCAVKVSKA
CCCCCCCEEEEEECCHHHHHHHHCCCCCCCCCCCCCCHHHEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA