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The map label for this gene is glyS

Identifier: 83311749

GI number: 83311749

Start: 2839778

End: 2841844

Strand: Reverse

Name: glyS

Synonym: amb2650

Alternate gene names: 83311749

Gene position: 2841844-2839778 (Counterclockwise)

Preceding gene: 83311750

Following gene: 83311748

Centisome position: 57.21

GC content: 66.81

Gene sequence:

>2067_bases
ATGGCCGAGTTCCTGCTTGAAATCTTCTCCGAGGAAATTCCCGCCCGCATGCAGGCCCGCGCCGCCGATGACCTGCGCGG
CATGGTCAGCGATGGTCTCGCCAAGAACGGCATCACCTTCGACGCGGCCCGGTCCTATGTCACGCCGCGCCGTCTGGTGC
TGGTGATCGAAGGCCTGCCCCTGGCCGGTCCCGACGTGTCGGAGGAAAAGCGCGGGCCGCGCGTCAGCTCCCCCGCCCAG
GCCATGGAGGGCTTTCTCAAGTCCACCGGCATGACGGTGGAACAGCTGGAGCAGCGCGACACCGGCAAGGGTGTGTTCTA
TTTCGCGGTGATCAACCGCAAGGGTCGTCCGACCGCCGAGGTTCTTCGGGAAGTGACCGAAGGCGCCATGGCCACCTTCC
CCTGGCCCAAGAGCCAGCGCTGGGGCGCCAATTCCATCCGCTGGGTGCGTCCCATCCAGTCCATCCTGGCCCTGTTCGAC
GGCTCGATCGTGCCGGTGGCCTTCGGCCCGGCCACGGCCGGCGACATGACCGCCGGTCATCGCTTCCTGGCCCCCGAGCC
GTTCCGGGTCAAGGACTTCGCGGATTATCTGTCCAAGCTGCGCCACGCCAAGGTGATGCTCGACCCCGTCGAGCGGCGCC
ACGCCATTCTGGCGGGGGCGGAAGGTCTGGCGGCGGCCGAGGGCCTTCAGCTCAAGGCTGATGACGGCCTGCTGGCCGAA
GTCACCGGTCTGGTTGAGTGGCCGGTGCCGCTGGTGGGCTCCATCGACGACAAGTTCATGGATGTGCCGGCCGAGGTGCT
GATCACCTCCATGCGCGCCCACCAGAAGTACTTCTCGCTGCTCAAGGCCGATGGCTCCCTGGCGCCGCGCTTCATCGTCA
TCTCCAACATGGAGACCGACGACGGGGGCAAGGCGGTGGTGGCGGGCAATCAGCGCGTGCTCCGCGCCCGCCTGTCCGAC
GCCAAGTTCTTCTGGGACACCGACCGCAAGCAGCGGCTGGAAGCCCGCCTGCCCAAGCTGGCCGAGCGCACCTTCTACGC
CAGCCTCGGCACGGTGGCCGACAAGGTGGACCGGATCTCGGCCCTGGCCGGCAACATCGCCGGCATGATCGGCGCCGACA
GGGCCGTGGCGGAACGGGCGGCCCGGTTGGCCAAGGCCGATCTCTCCACCGAACTGGTGGGCGAATTCCCTGAGCTACAG
GGCCTGATGGGGCGCTATTACGCGCTCAACGACGGCGAGACGCCCGAGGTGGCCGATGCCATCGCCGCCCATTACTCACC
CCAAGGGCCGGGCGATTCCTGCCCCACCGCGCCGGTATCCGTGGCCGTGGCCCTGGCCGACAAGATTGATTCCCTGGTGG
GATTCTTCGCCATCAACGAGAAACCCACGGGTTCCAAGGACCCCTTCGCGCTGCGCCGCGCCGCGCTGGGCATCATCCGT
CTGGTGTTGGAGAACGGCCTGCGCCTGCCGCTGTCCCAGGTCATTTTCCTGGCCTCGGGTCACTACAAGGCCAATCTGGT
GAATTCGCCGGGCGAGGTGGCCAACGATCTGCTGAACTTCTTCGCCGACCGCTTGGCGGTGGCGCTGAAGGAAAAGGGCG
TGCGTCATGACCTCATCACCGCCATCTTCTCCCTGGGGGGCGAGGATGATCTGGTCCGCCTGCTGAAGCGGGTCGAGGCC
CTGGGCGCCTTCCTGGACTCCGACGACGGCGCCAACCTGCTGATCGCCTATCGTCGCGCTGCCAATATCGTGCGCATCGA
GGAGAAGAAGGACGGGACGATCTTCTCGGGTTACGCCGACATCCAACTGCTGCGCCAGGACGAGGAGAAGGCGCTTGAGC
ACGCCTTGAGCGATGTCGGCCAGGCAGTGAGCAATGCCCTGGTCACCGAGGATTTTGCCGCCGCCATGGCGGCCCTGGCC
CGCTTGCGCCGCCCGGTGGACGCCTTTTTCGACAAGGTGACGGTCAATGCCGACGAGGCGCCCCTGCGCGTCAACCGCCT
GAAGCTGCTGGCCATGATCGGCTCGGCCATGGGCCGGCTGGCCGACTTCTCCAAGGTGGAAGGCTAG

Upstream 100 bases:

>100_bases
ACCGAGCGCCAGGCCTATATCGGCCGCGTCCGTGCCCTGGCCAAGGCCTGCTGCGAAGGCTGGCTGGCTTCCCGCACCAC
CGGAAAGGAGGGCTGAAGCC

Downstream 100 bases:

>100_bases
CGCCGAGCGTTAAAAGCAGCACTACCTATTTTCAATCCCTCTGGGACGTTGCATGATGAAGCCCGCGCCGCACTCTGTGG
CGCGGGTTTCCTTTTTACGG

Product: glycyl-tRNA synthetase

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 688; Mature: 687

Protein sequence:

>688_residues
MAEFLLEIFSEEIPARMQARAADDLRGMVSDGLAKNGITFDAARSYVTPRRLVLVIEGLPLAGPDVSEEKRGPRVSSPAQ
AMEGFLKSTGMTVEQLEQRDTGKGVFYFAVINRKGRPTAEVLREVTEGAMATFPWPKSQRWGANSIRWVRPIQSILALFD
GSIVPVAFGPATAGDMTAGHRFLAPEPFRVKDFADYLSKLRHAKVMLDPVERRHAILAGAEGLAAAEGLQLKADDGLLAE
VTGLVEWPVPLVGSIDDKFMDVPAEVLITSMRAHQKYFSLLKADGSLAPRFIVISNMETDDGGKAVVAGNQRVLRARLSD
AKFFWDTDRKQRLEARLPKLAERTFYASLGTVADKVDRISALAGNIAGMIGADRAVAERAARLAKADLSTELVGEFPELQ
GLMGRYYALNDGETPEVADAIAAHYSPQGPGDSCPTAPVSVAVALADKIDSLVGFFAINEKPTGSKDPFALRRAALGIIR
LVLENGLRLPLSQVIFLASGHYKANLVNSPGEVANDLLNFFADRLAVALKEKGVRHDLITAIFSLGGEDDLVRLLKRVEA
LGAFLDSDDGANLLIAYRRAANIVRIEEKKDGTIFSGYADIQLLRQDEEKALEHALSDVGQAVSNALVTEDFAAAMAALA
RLRRPVDAFFDKVTVNADEAPLRVNRLKLLAMIGSAMGRLADFSKVEG

Sequences:

>Translated_688_residues
MAEFLLEIFSEEIPARMQARAADDLRGMVSDGLAKNGITFDAARSYVTPRRLVLVIEGLPLAGPDVSEEKRGPRVSSPAQ
AMEGFLKSTGMTVEQLEQRDTGKGVFYFAVINRKGRPTAEVLREVTEGAMATFPWPKSQRWGANSIRWVRPIQSILALFD
GSIVPVAFGPATAGDMTAGHRFLAPEPFRVKDFADYLSKLRHAKVMLDPVERRHAILAGAEGLAAAEGLQLKADDGLLAE
VTGLVEWPVPLVGSIDDKFMDVPAEVLITSMRAHQKYFSLLKADGSLAPRFIVISNMETDDGGKAVVAGNQRVLRARLSD
AKFFWDTDRKQRLEARLPKLAERTFYASLGTVADKVDRISALAGNIAGMIGADRAVAERAARLAKADLSTELVGEFPELQ
GLMGRYYALNDGETPEVADAIAAHYSPQGPGDSCPTAPVSVAVALADKIDSLVGFFAINEKPTGSKDPFALRRAALGIIR
LVLENGLRLPLSQVIFLASGHYKANLVNSPGEVANDLLNFFADRLAVALKEKGVRHDLITAIFSLGGEDDLVRLLKRVEA
LGAFLDSDDGANLLIAYRRAANIVRIEEKKDGTIFSGYADIQLLRQDEEKALEHALSDVGQAVSNALVTEDFAAAMAALA
RLRRPVDAFFDKVTVNADEAPLRVNRLKLLAMIGSAMGRLADFSKVEG
>Mature_687_residues
AEFLLEIFSEEIPARMQARAADDLRGMVSDGLAKNGITFDAARSYVTPRRLVLVIEGLPLAGPDVSEEKRGPRVSSPAQA
MEGFLKSTGMTVEQLEQRDTGKGVFYFAVINRKGRPTAEVLREVTEGAMATFPWPKSQRWGANSIRWVRPIQSILALFDG
SIVPVAFGPATAGDMTAGHRFLAPEPFRVKDFADYLSKLRHAKVMLDPVERRHAILAGAEGLAAAEGLQLKADDGLLAEV
TGLVEWPVPLVGSIDDKFMDVPAEVLITSMRAHQKYFSLLKADGSLAPRFIVISNMETDDGGKAVVAGNQRVLRARLSDA
KFFWDTDRKQRLEARLPKLAERTFYASLGTVADKVDRISALAGNIAGMIGADRAVAERAARLAKADLSTELVGEFPELQG
LMGRYYALNDGETPEVADAIAAHYSPQGPGDSCPTAPVSVAVALADKIDSLVGFFAINEKPTGSKDPFALRRAALGIIRL
VLENGLRLPLSQVIFLASGHYKANLVNSPGEVANDLLNFFADRLAVALKEKGVRHDLITAIFSLGGEDDLVRLLKRVEAL
GAFLDSDDGANLLIAYRRAANIVRIEEKKDGTIFSGYADIQLLRQDEEKALEHALSDVGQAVSNALVTEDFAAAMAALAR
LRRPVDAFFDKVTVNADEAPLRVNRLKLLAMIGSAMGRLADFSKVEG

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=689, Percent_Identity=37.155297532656, Blast_Score=422, Evalue=1e-119,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_MAGSA (Q2W3X1)

Other databases:

- EMBL:   AP007255
- RefSeq:   YP_422013.1
- ProteinModelPortal:   Q2W3X1
- STRING:   Q2W3X1
- GeneID:   3804758
- GenomeReviews:   AP007255_GR
- KEGG:   mag:amb2650
- NMPDR:   fig|342108.5.peg.2346
- eggNOG:   COG0751
- HOGENOM:   HBG586599
- OMA:   SNITYGH
- PhylomeDB:   Q2W3X1
- ProtClustDB:   PRK01233
- BioCyc:   MMAG342108:AMB2650-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 74505; Mature: 74374

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEFLLEIFSEEIPARMQARAADDLRGMVSDGLAKNGITFDAARSYVTPRRLVLVIEGLP
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHCCCCCEEEEEEECCC
LAGPDVSEEKRGPRVSSPAQAMEGFLKSTGMTVEQLEQRDTGKGVFYFAVINRKGRPTAE
CCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEEECCCCCCHHH
VLREVTEGAMATFPWPKSQRWGANSIRWVRPIQSILALFDGSIVPVAFGPATAGDMTAGH
HHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCC
RFLAPEPFRVKDFADYLSKLRHAKVMLDPVERRHAILAGAEGLAAAEGLQLKADDGLLAE
CCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCHHHHCCCEEECCCCHHHH
VTGLVEWPVPLVGSIDDKFMDVPAEVLITSMRAHQKYFSLLKADGSLAPRFIVISNMETD
HHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
DGGKAVVAGNQRVLRARLSDAKFFWDTDRKQRLEARLPKLAERTFYASLGTVADKVDRIS
CCCEEEEECCHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAGNIAGMIGADRAVAERAARLAKADLSTELVGEFPELQGLMGRYYALNDGETPEVADA
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHEEEECCCCCHHHHHH
IAAHYSPQGPGDSCPTAPVSVAVALADKIDSLVGFFAINEKPTGSKDPFALRRAALGIIR
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHH
LVLENGLRLPLSQVIFLASGHYKANLVNSPGEVANDLLNFFADRLAVALKEKGVRHDLIT
HHHHCCCCCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
AIFSLGGEDDLVRLLKRVEALGAFLDSDDGANLLIAYRRAANIVRIEEKKDGTIFSGYAD
HHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHCCEEEEEECCCCCEEECHHH
IQLLRQDEEKALEHALSDVGQAVSNALVTEDFAAAMAALARLRRPVDAFFDKVTVNADEA
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHEECCCCCC
PLRVNRLKLLAMIGSAMGRLADFSKVEG
CHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AEFLLEIFSEEIPARMQARAADDLRGMVSDGLAKNGITFDAARSYVTPRRLVLVIEGLP
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHCCCCCEEEEEEECCC
LAGPDVSEEKRGPRVSSPAQAMEGFLKSTGMTVEQLEQRDTGKGVFYFAVINRKGRPTAE
CCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEEECCCCCCHHH
VLREVTEGAMATFPWPKSQRWGANSIRWVRPIQSILALFDGSIVPVAFGPATAGDMTAGH
HHHHHHCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCC
RFLAPEPFRVKDFADYLSKLRHAKVMLDPVERRHAILAGAEGLAAAEGLQLKADDGLLAE
CCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCHHHHCCCEEECCCCHHHH
VTGLVEWPVPLVGSIDDKFMDVPAEVLITSMRAHQKYFSLLKADGSLAPRFIVISNMETD
HHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
DGGKAVVAGNQRVLRARLSDAKFFWDTDRKQRLEARLPKLAERTFYASLGTVADKVDRIS
CCCEEEEECCHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAGNIAGMIGADRAVAERAARLAKADLSTELVGEFPELQGLMGRYYALNDGETPEVADA
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHEEEECCCCCHHHHHH
IAAHYSPQGPGDSCPTAPVSVAVALADKIDSLVGFFAINEKPTGSKDPFALRRAALGIIR
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHH
LVLENGLRLPLSQVIFLASGHYKANLVNSPGEVANDLLNFFADRLAVALKEKGVRHDLIT
HHHHCCCCCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
AIFSLGGEDDLVRLLKRVEALGAFLDSDDGANLLIAYRRAANIVRIEEKKDGTIFSGYAD
HHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHCCEEEEEECCCCCEEECHHH
IQLLRQDEEKALEHALSDVGQAVSNALVTEDFAAAMAALARLRRPVDAFFDKVTVNADEA
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHEECCCCCC
PLRVNRLKLLAMIGSAMGRLADFSKVEG
CHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA