Click here to switch to the map view.

The map label for this gene is ppdK [H]

Identifier: 83311748

GI number: 83311748

Start: 2836974

End: 2839658

Strand: Reverse

Name: ppdK [H]

Synonym: amb2649

Alternate gene names: 83311748

Gene position: 2839658-2836974 (Counterclockwise)

Preceding gene: 83311749

Following gene: 83311747

Centisome position: 57.17

GC content: 65.51

Gene sequence:

>2685_bases
ATGACGAAATGGGTCTACAGCTTCGGTGGCGGCAAGGCGGAAGGTCGGGCCGACATGAAGAACCTGCTGGGCGGCGAGGG
TGCGAACCTGGCGGAGATGAGCAATCTCGGCATTCCGGTTCCCCCCGGTTTCACCATCAGTACCGAGGTCTGTACCTATT
ACTACGGCAATGGCGAGACCTATCCCGCCACCCTCAAGGACGAGGTGATGGCTGCCCTGTCCCAGGTGGAGGAGGATATC
GGCCTGAAGTTCGGCTCCACCGCCGACCCGCTGCTGGTCTCGGTGCGCTCGGGCGCCCGGGCCTCCATGCCGGGCATGAT
GGATACCATCCTCAATCTCGGCCTCAACGACAAGTCGGTGGAGGGTCTGGCCAAGCGGTCCAATGATGCCCGCTTCGCCT
ATGACAGCTATCGCCGCTTCATCCAGATGTATTCCAACGTGGTGTTGGGCGTCGACCATCACAATTTCGAGGAGATCCTC
GACGAGGTGAAGGAAGACAAGGGCGTCAGCCTCGATACCGAGCTGGATGCCGACGACCTGAAGAAGGTGGTCGCCAAGTA
CAAGGACAAGGTGGCGGAACAGCTGGGCAAGCCGTTCCCCCAGGATGTGCACGAGCAGCTTTGGGGCGCCGTCGGCGCGG
TGTTCGGTTCGTGGATGAACCAGCGCGCCATCACCTATCGCCGCCTGCACGACATTCCCGCCGAATGGGGCACCGCCGTC
AACGTGCAGTCCATGGTGTTCGGCAACATGGGCAATGACTGCTCCACCGGCGTGTGCTTCACCCGCAACCCGTCTACCGG
CGAGAACGCCTTCTATGGCGAGTTCCTGGTCAATGCCCAGGGCGAGGACGTGGTGGCCGGCATCCGCACCCCGCAGCAGC
TGACCATCGCCGGCAAGAACGCCCAGTCGTCGGACCTGCCGGCCATGGAAGAGTGCATGCCCGAGGTGTTCAAGGAACTG
AACGCCATCCGCCACAAGCTGGAAGCCCATTACCGCGACATGCAGGACATGGAGTTCACGGTTCAGCAGAACCGCCTGTG
GATGCTCCAGACCCGCACGGGCAAGCGCACCACCAACGCGGCGCTGAAGATCGCCGTGGACATGGCGCGCGAGGGCCTGA
TCAATCGCAAGGACGCCATCAAGCGCATCGATCCCGCCTCGCTGGACCAGTTGCTGCACCCGACCCTGGACCCCAAGGCG
CCGCGCACCCTGCTGGGCCGCGGCCTGCCGGCCTCGCCGGGCGCCGCGTCGGGCAAGGTGGTGTTCTCCGCCGATGAGGC
CGAGGACTGGGTCAAGAACCGCAAGGAGCGCGTCATCCTGGTGCGCATCGAGACGTCGCCTGAGGATATCGGCGGCATGC
ATGTCTCGGAAGGCATCCTGACCACCCGTGGCGGCATGACCAGCCACGCCGCCGTGGTGGCGCGCGGCATGGGCACGCCC
TGCGTCGCCGGTGCCGGCGAGATCCGCGTCGACTACGCCTCCAAGACCCTCAAGGTGGCGGGCAAGGTGGTCAAGGAAGG
GGATACCATCACCCTGGACGGCTCCACCGGTGAAGTCTTCATCGGCGCCGTGGCGACCATCCAGCCCGAGCTGACCGGTG
ATTTCGGCACGCTGATGGAGTGGGTGGACACCATCCGCACCCTGAAGGTCCGCGCCAATGCCGAGACGCCGCTGGACGCC
GCCACGGCGCGCAAGTTCGGCGCCGAGGGCATCGGCCTGTGCCGGACCGAGCACATGTTCTTCGATCCCAAGCGCATCAT
CGCCATGCGCGAGATGATCCTGGCCGAGACCGAGAAGGGTCGCCGCGCCGCCCTGGCCAAGCTGCTGCCCTATCAGCGCC
AGGACTTCATCGAGCTGTTCGAGATCATGCAGGGACTGCCGGTGACCATCCGCCTGCTGGACCCGCCGCTGCACGAGTTC
CTACCCAATTCCGAGGCCGAGATCGCCGAAGTGGCGAAGGAAGCCGGGGTGGAGCCCGCCGTGGTCAAGGCCCGCAACGC
GGCGCTGCACGAGAACAACCCCATGCTGGGCCATCGTGGCTGCCGTCTGGGCATCACCTATCCGGAAATCTACGAGATGC
AGGCCCGGGCCATCTTCGAGGCGGCGGTGCATGTGTCGCGTGATACCGGCCGCACCGTGGTGCCGGAGATCATGATCCCC
CTGGTGGGCGCCAAGAAGGAGCTGGACCTGATGAAGGCCAGCATCGACAAGGAAGCCGAGGCGGTGTTCGCCGAGCAGAA
ATACTCGGTGCATTACATGGTCGGCACCATGATCGAGCTGCCGCGTGCCGCTCTGCTGGCCGGTGACATCGCCCAGACCG
GCGAGTTCTTCTCGTTCGGCACCAACGATCTCACCCAGACCACCTTCGGCATGAGCCGCGACGATTCCGGCCCGTTCCTC
GAGGTCTACCGCGCCAAGGGCATCTACGAGCACGACCCCTTCGCCAGCCTGGATCAGGCCGGCGTGGGCGAACTGATCAA
GATCGCCGCCGAGCGTGGCCGTGCCACCCGGCCCGGCCTCAAGCTCGGTATCTGCGGTGAGCATGGCGGCGACCCGGCCT
CCATCCACTTCTGCCAGGGAGCGGGTCTGGATTACGTGTCGTGTTCGCCCTACCGCGTGCCGATCGCCCGTCTGGCGGCC
GCCCAGGCGGCCCTGGGTGGCTCGTCCTCCTCCGGGACGGCCTGA

Upstream 100 bases:

>100_bases
CACTACCTATTTTCAATCCCTCTGGGACGTTGCATGATGAAGCCCGCGCCGCACTCTGTGGCGCGGGTTTCCTTTTTACG
GATCGGGGATGAGAACGGCC

Downstream 100 bases:

>100_bases
CGAAAAAGGAAAAGGGTCCTTCGGGGCCCTTTTTCAATTCCAGGACTGGAAACCCAAGGTGCAACATACTAAAGTAAAAG
GGGGGGCTTTAGGAGATGCA

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 894; Mature: 893

Protein sequence:

>894_residues
MTKWVYSFGGGKAEGRADMKNLLGGEGANLAEMSNLGIPVPPGFTISTEVCTYYYGNGETYPATLKDEVMAALSQVEEDI
GLKFGSTADPLLVSVRSGARASMPGMMDTILNLGLNDKSVEGLAKRSNDARFAYDSYRRFIQMYSNVVLGVDHHNFEEIL
DEVKEDKGVSLDTELDADDLKKVVAKYKDKVAEQLGKPFPQDVHEQLWGAVGAVFGSWMNQRAITYRRLHDIPAEWGTAV
NVQSMVFGNMGNDCSTGVCFTRNPSTGENAFYGEFLVNAQGEDVVAGIRTPQQLTIAGKNAQSSDLPAMEECMPEVFKEL
NAIRHKLEAHYRDMQDMEFTVQQNRLWMLQTRTGKRTTNAALKIAVDMAREGLINRKDAIKRIDPASLDQLLHPTLDPKA
PRTLLGRGLPASPGAASGKVVFSADEAEDWVKNRKERVILVRIETSPEDIGGMHVSEGILTTRGGMTSHAAVVARGMGTP
CVAGAGEIRVDYASKTLKVAGKVVKEGDTITLDGSTGEVFIGAVATIQPELTGDFGTLMEWVDTIRTLKVRANAETPLDA
ATARKFGAEGIGLCRTEHMFFDPKRIIAMREMILAETEKGRRAALAKLLPYQRQDFIELFEIMQGLPVTIRLLDPPLHEF
LPNSEAEIAEVAKEAGVEPAVVKARNAALHENNPMLGHRGCRLGITYPEIYEMQARAIFEAAVHVSRDTGRTVVPEIMIP
LVGAKKELDLMKASIDKEAEAVFAEQKYSVHYMVGTMIELPRAALLAGDIAQTGEFFSFGTNDLTQTTFGMSRDDSGPFL
EVYRAKGIYEHDPFASLDQAGVGELIKIAAERGRATRPGLKLGICGEHGGDPASIHFCQGAGLDYVSCSPYRVPIARLAA
AQAALGGSSSSGTA

Sequences:

>Translated_894_residues
MTKWVYSFGGGKAEGRADMKNLLGGEGANLAEMSNLGIPVPPGFTISTEVCTYYYGNGETYPATLKDEVMAALSQVEEDI
GLKFGSTADPLLVSVRSGARASMPGMMDTILNLGLNDKSVEGLAKRSNDARFAYDSYRRFIQMYSNVVLGVDHHNFEEIL
DEVKEDKGVSLDTELDADDLKKVVAKYKDKVAEQLGKPFPQDVHEQLWGAVGAVFGSWMNQRAITYRRLHDIPAEWGTAV
NVQSMVFGNMGNDCSTGVCFTRNPSTGENAFYGEFLVNAQGEDVVAGIRTPQQLTIAGKNAQSSDLPAMEECMPEVFKEL
NAIRHKLEAHYRDMQDMEFTVQQNRLWMLQTRTGKRTTNAALKIAVDMAREGLINRKDAIKRIDPASLDQLLHPTLDPKA
PRTLLGRGLPASPGAASGKVVFSADEAEDWVKNRKERVILVRIETSPEDIGGMHVSEGILTTRGGMTSHAAVVARGMGTP
CVAGAGEIRVDYASKTLKVAGKVVKEGDTITLDGSTGEVFIGAVATIQPELTGDFGTLMEWVDTIRTLKVRANAETPLDA
ATARKFGAEGIGLCRTEHMFFDPKRIIAMREMILAETEKGRRAALAKLLPYQRQDFIELFEIMQGLPVTIRLLDPPLHEF
LPNSEAEIAEVAKEAGVEPAVVKARNAALHENNPMLGHRGCRLGITYPEIYEMQARAIFEAAVHVSRDTGRTVVPEIMIP
LVGAKKELDLMKASIDKEAEAVFAEQKYSVHYMVGTMIELPRAALLAGDIAQTGEFFSFGTNDLTQTTFGMSRDDSGPFL
EVYRAKGIYEHDPFASLDQAGVGELIKIAAERGRATRPGLKLGICGEHGGDPASIHFCQGAGLDYVSCSPYRVPIARLAA
AQAALGGSSSSGTA
>Mature_893_residues
TKWVYSFGGGKAEGRADMKNLLGGEGANLAEMSNLGIPVPPGFTISTEVCTYYYGNGETYPATLKDEVMAALSQVEEDIG
LKFGSTADPLLVSVRSGARASMPGMMDTILNLGLNDKSVEGLAKRSNDARFAYDSYRRFIQMYSNVVLGVDHHNFEEILD
EVKEDKGVSLDTELDADDLKKVVAKYKDKVAEQLGKPFPQDVHEQLWGAVGAVFGSWMNQRAITYRRLHDIPAEWGTAVN
VQSMVFGNMGNDCSTGVCFTRNPSTGENAFYGEFLVNAQGEDVVAGIRTPQQLTIAGKNAQSSDLPAMEECMPEVFKELN
AIRHKLEAHYRDMQDMEFTVQQNRLWMLQTRTGKRTTNAALKIAVDMAREGLINRKDAIKRIDPASLDQLLHPTLDPKAP
RTLLGRGLPASPGAASGKVVFSADEAEDWVKNRKERVILVRIETSPEDIGGMHVSEGILTTRGGMTSHAAVVARGMGTPC
VAGAGEIRVDYASKTLKVAGKVVKEGDTITLDGSTGEVFIGAVATIQPELTGDFGTLMEWVDTIRTLKVRANAETPLDAA
TARKFGAEGIGLCRTEHMFFDPKRIIAMREMILAETEKGRRAALAKLLPYQRQDFIELFEIMQGLPVTIRLLDPPLHEFL
PNSEAEIAEVAKEAGVEPAVVKARNAALHENNPMLGHRGCRLGITYPEIYEMQARAIFEAAVHVSRDTGRTVVPEIMIPL
VGAKKELDLMKASIDKEAEAVFAEQKYSVHYMVGTMIELPRAALLAGDIAQTGEFFSFGTNDLTQTTFGMSRDDSGPFLE
VYRAKGIYEHDPFASLDQAGVGELIKIAAERGRATRPGLKLGICGEHGGDPASIHFCQGAGLDYVSCSPYRVPIARLAAA
QAALGGSSSSGTA

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=460, Percent_Identity=25.2173913043478, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1787994, Length=715, Percent_Identity=25.8741258741259, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1789193, Length=477, Percent_Identity=26.2054507337526, Blast_Score=103, Evalue=5e-23,
Organism=Escherichia coli, GI48994992, Length=441, Percent_Identity=25.1700680272109, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1788726, Length=648, Percent_Identity=21.141975308642, Blast_Score=77, Evalue=5e-15,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 97167; Mature: 97036

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKWVYSFGGGKAEGRADMKNLLGGEGANLAEMSNLGIPVPPGFTISTEVCTYYYGNGET
CCCEEEECCCCCCCCHHHHHHHHCCCCCCHHHHHCCCCCCCCCCEEEEEEEEEEECCCCC
YPATLKDEVMAALSQVEEDIGLKFGSTADPLLVSVRSGARASMPGMMDTILNLGLNDKSV
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCHHH
EGLAKRSNDARFAYDSYRRFIQMYSNVVLGVDHHNFEEILDEVKEDKGVSLDTELDADDL
HHHHHCCCCCCCHHHHHHHHHHHHHCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHH
KKVVAKYKDKVAEQLGKPFPQDVHEQLWGAVGAVFGSWMNQRAITYRRLHDIPAEWGTAV
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCC
NVQSMVFGNMGNDCSTGVCFTRNPSTGENAFYGEFLVNAQGEDVVAGIRTPQQLTIAGKN
EEHHHHCCCCCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCCEEEECCCCCEEEEECCC
AQSSDLPAMEECMPEVFKELNAIRHKLEAHYRDMQDMEFTVQQNRLWMLQTRTGKRTTNA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEECCEEEEEEECCCCCCCCH
ALKIAVDMAREGLINRKDAIKRIDPASLDQLLHPTLDPKAPRTLLGRGLPASPGAASGKV
HHEEHHHHHHHCCCCHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCEE
VFSADEAEDWVKNRKERVILVRIETSPEDIGGMHVSEGILTTRGGMTSHAAVVARGMGTP
EEECCCHHHHHHCCCCEEEEEEEECCCCHHCCEEECCCEEEECCCCCHHHHHHHHCCCCC
CVAGAGEIRVDYASKTLKVAGKVVKEGDTITLDGSTGEVFIGAVATIQPELTGDFGTLME
CCCCCCCEEEEHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEEECCCCCCCHHHHHH
WVDTIRTLKVRANAETPLDAATARKFGAEGIGLCRTEHMFFDPKRIIAMREMILAETEKG
HHHHHHEEEEEECCCCCCHHHHHHHHCCCCCCEEEECCEEECHHHHHHHHHHHHHHCCCC
RRAALAKLLPYQRQDFIELFEIMQGLPVTIRLLDPPLHEFLPNSEAEIAEVAKEAGVEPA
HHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCHHHHHHHHHCCCCHH
VVKARNAALHENNPMLGHRGCRLGITYPEIYEMQARAIFEAAVHVSRDTGRTVVPEIMIP
HHHHCCCHHCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHH
LVGAKKELDLMKASIDKEAEAVFAEQKYSVHYMVGTMIELPRAALLAGDIAQTGEFFSFG
HCCCHHHHHHHHHHCCHHHHHHHHHCCCEEEEHHHHHHHCCHHHHHHCCHHHCCCHHCCC
TNDLTQTTFGMSRDDSGPFLEVYRAKGIYEHDPFASLDQAGVGELIKIAAERGRATRPGL
CCCHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCC
KLGICGEHGGDPASIHFCQGAGLDYVSCSPYRVPIARLAAAQAALGGSSSSGTA
EEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TKWVYSFGGGKAEGRADMKNLLGGEGANLAEMSNLGIPVPPGFTISTEVCTYYYGNGET
CCEEEECCCCCCCCHHHHHHHHCCCCCCHHHHHCCCCCCCCCCEEEEEEEEEEECCCCC
YPATLKDEVMAALSQVEEDIGLKFGSTADPLLVSVRSGARASMPGMMDTILNLGLNDKSV
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCHHH
EGLAKRSNDARFAYDSYRRFIQMYSNVVLGVDHHNFEEILDEVKEDKGVSLDTELDADDL
HHHHHCCCCCCCHHHHHHHHHHHHHCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHH
KKVVAKYKDKVAEQLGKPFPQDVHEQLWGAVGAVFGSWMNQRAITYRRLHDIPAEWGTAV
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCC
NVQSMVFGNMGNDCSTGVCFTRNPSTGENAFYGEFLVNAQGEDVVAGIRTPQQLTIAGKN
EEHHHHCCCCCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCCEEEECCCCCEEEEECCC
AQSSDLPAMEECMPEVFKELNAIRHKLEAHYRDMQDMEFTVQQNRLWMLQTRTGKRTTNA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEECCEEEEEEECCCCCCCCH
ALKIAVDMAREGLINRKDAIKRIDPASLDQLLHPTLDPKAPRTLLGRGLPASPGAASGKV
HHEEHHHHHHHCCCCHHHHHHHCCHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCCEE
VFSADEAEDWVKNRKERVILVRIETSPEDIGGMHVSEGILTTRGGMTSHAAVVARGMGTP
EEECCCHHHHHHCCCCEEEEEEEECCCCHHCCEEECCCEEEECCCCCHHHHHHHHCCCCC
CVAGAGEIRVDYASKTLKVAGKVVKEGDTITLDGSTGEVFIGAVATIQPELTGDFGTLME
CCCCCCCEEEEHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEEECCCCCCCHHHHHH
WVDTIRTLKVRANAETPLDAATARKFGAEGIGLCRTEHMFFDPKRIIAMREMILAETEKG
HHHHHHEEEEEECCCCCCHHHHHHHHCCCCCCEEEECCEEECHHHHHHHHHHHHHHCCCC
RRAALAKLLPYQRQDFIELFEIMQGLPVTIRLLDPPLHEFLPNSEAEIAEVAKEAGVEPA
HHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCHHHHHHHHHCCCCHH
VVKARNAALHENNPMLGHRGCRLGITYPEIYEMQARAIFEAAVHVSRDTGRTVVPEIMIP
HHHHCCCHHCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHH
LVGAKKELDLMKASIDKEAEAVFAEQKYSVHYMVGTMIELPRAALLAGDIAQTGEFFSFG
HCCCHHHHHHHHHHCCHHHHHHHHHCCCEEEEHHHHHHHCCHHHHHHCCHHHCCCHHCCC
TNDLTQTTFGMSRDDSGPFLEVYRAKGIYEHDPFASLDQAGVGELIKIAAERGRATRPGL
CCCHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCC
KLGICGEHGGDPASIHFCQGAGLDYVSCSPYRVPIARLAAAQAALGGSSSSGTA
EEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430; 9168612 [H]