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The map label for this gene is potF [H]

Identifier: 83311724

GI number: 83311724

Start: 2811027

End: 2812136

Strand: Reverse

Name: potF [H]

Synonym: amb2625

Alternate gene names: 83311724

Gene position: 2812136-2811027 (Counterclockwise)

Preceding gene: 83311725

Following gene: 83311723

Centisome position: 56.61

GC content: 62.97

Gene sequence:

>1110_bases
ATGCGCATTTCCTTTACGGCTGTCGCGGTGTCGCTGATTGCCGCGGCTGTGTCTTCGGTTTCCGGAGCCCAGGCGGCGGA
AGACAAGGTGCTGAACGTCTACAACTGGTCGGACTACATCGCGCCCGACACGCTGGAGAAGTTCACGGCCCTGACCGGCA
TTAAGGTCAATTACGACGTCTACGACAGCAACGAGGTGCTGCAGGCCAAGCTGCAGGCCGGCAAGTCGGGCTATGACGTG
GTGTTCCCCTCGGCTTCGCCCTTCCTGGCCAACCAGATCAAGGCCGGCATCTACCGCAAGCTCGATCGCTCCAAGCTCAG
CAATTACGGCAACCTGGACGCCCAGGTGATGGTGACCCTGAACCGGGCCGCCGATCCCGGCAACCTCTACGCCATTCCCT
ATGCCATCTCGCCCACCGGCTTCGCCTACAACGTGGACAAAGTGGCCAAGGCGGGCAAGGACATGCCCCTGGACAGCTGG
GCGGCGCTGTTCGACCCGGCCCAGGTCGCCAAGCTGAAGGGCTGCGGCGTCAGCCTGCTTGACGCTCCCACCGAGGTGTT
TCCGGCGGCCCTGACCTATCTGGGCAAGAACGGCGCCTCGCTGGACTCCGCCGATCTCAAGGCGGCGGCCGAGGCGGTGA
CCAAGGTGCGGCCCAGCATCAAGTATTTCCACTCGTCCAAGTTCATCAACGATCTGGCCAATGGCGACATCTGCATGGCG
CACGGCTATGTGGGCGATCTGGTCCAGTCGCGCAACCGTGCCGCCGAGGCCGGCAAGGGCGTCAACATCAAGATCGTCAT
TCCCAAGGAAGGCGCGGTGATCAACATCGATTCCATGGTGATCCCCGCCGATGCGCCCCATCCGGAAAACGCGCACAAGT
TCATCGATTTCATGATGAAGCAGGAGGTGGCGGCGGGCTTTTCCAACACCACCGGCTACGGCAATCCCATTCCGGGGTCG
CGGAGCCTGATCCGCAAGGAAATCCAGGAGGACCCGGTGATCTTCCCGTCCGAGCAGGTGACGGCCAAGCTGTACCAGGT
TCCGCCGGCGGACATGGCCAAGGAGCGCGAGCGCACCCGCCTGTGGACCACCGTCAAAACGGGTCGCTGA

Upstream 100 bases:

>100_bases
TTGCCCGTTGACAAGGCACTCCCGGGACCGGAAATATCGGCCCCGCGCGGGACGGCGGCGAGCGGCCCGGCATCAGGCTT
CCTGGCTGGGGGACAAGGTC

Downstream 100 bases:

>100_bases
GGGCATGGCGCAGGCGGCGGTCAAGCGGGAGGTGACGGCTCCCTGGAACGATCCCCAGGCGGTCCCGCTGATCCGCTTTG
AAGGAATATCCAAGCGGTTC

Product: spermidine/putrescine-binding periplasmic protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MRISFTAVAVSLIAAAVSSVSGAQAAEDKVLNVYNWSDYIAPDTLEKFTALTGIKVNYDVYDSNEVLQAKLQAGKSGYDV
VFPSASPFLANQIKAGIYRKLDRSKLSNYGNLDAQVMVTLNRAADPGNLYAIPYAISPTGFAYNVDKVAKAGKDMPLDSW
AALFDPAQVAKLKGCGVSLLDAPTEVFPAALTYLGKNGASLDSADLKAAAEAVTKVRPSIKYFHSSKFINDLANGDICMA
HGYVGDLVQSRNRAAEAGKGVNIKIVIPKEGAVINIDSMVIPADAPHPENAHKFIDFMMKQEVAAGFSNTTGYGNPIPGS
RSLIRKEIQEDPVIFPSEQVTAKLYQVPPADMAKERERTRLWTTVKTGR

Sequences:

>Translated_369_residues
MRISFTAVAVSLIAAAVSSVSGAQAAEDKVLNVYNWSDYIAPDTLEKFTALTGIKVNYDVYDSNEVLQAKLQAGKSGYDV
VFPSASPFLANQIKAGIYRKLDRSKLSNYGNLDAQVMVTLNRAADPGNLYAIPYAISPTGFAYNVDKVAKAGKDMPLDSW
AALFDPAQVAKLKGCGVSLLDAPTEVFPAALTYLGKNGASLDSADLKAAAEAVTKVRPSIKYFHSSKFINDLANGDICMA
HGYVGDLVQSRNRAAEAGKGVNIKIVIPKEGAVINIDSMVIPADAPHPENAHKFIDFMMKQEVAAGFSNTTGYGNPIPGS
RSLIRKEIQEDPVIFPSEQVTAKLYQVPPADMAKERERTRLWTTVKTGR
>Mature_369_residues
MRISFTAVAVSLIAAAVSSVSGAQAAEDKVLNVYNWSDYIAPDTLEKFTALTGIKVNYDVYDSNEVLQAKLQAGKSGYDV
VFPSASPFLANQIKAGIYRKLDRSKLSNYGNLDAQVMVTLNRAADPGNLYAIPYAISPTGFAYNVDKVAKAGKDMPLDSW
AALFDPAQVAKLKGCGVSLLDAPTEVFPAALTYLGKNGASLDSADLKAAAEAVTKVRPSIKYFHSSKFINDLANGDICMA
HGYVGDLVQSRNRAAEAGKGVNIKIVIPKEGAVINIDSMVIPADAPHPENAHKFIDFMMKQEVAAGFSNTTGYGNPIPGS
RSLIRKEIQEDPVIFPSEQVTAKLYQVPPADMAKERERTRLWTTVKTGR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=361, Percent_Identity=48.4764542936288, Blast_Score=341, Evalue=4e-95,
Organism=Escherichia coli, GI1787367, Length=322, Percent_Identity=35.4037267080745, Blast_Score=184, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39730; Mature: 39730

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRISFTAVAVSLIAAAVSSVSGAQAAEDKVLNVYNWSDYIAPDTLEKFTALTGIKVNYDV
CEEEHHHHHHHHHHHHHHHCCCCHHHHCCEEEEECCCCCCCCHHHHHHHHHHCEEEEEEE
YDSNEVLQAKLQAGKSGYDVVFPSASPFLANQIKAGIYRKLDRSKLSNYGNLDAQVMVTL
ECCHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
NRAADPGNLYAIPYAISPTGFAYNVDKVAKAGKDMPLDSWAALFDPAQVAKLKGCGVSLL
ECCCCCCCEEEEEEEECCCCCEECHHHHHHCCCCCCCHHHHHHCCHHHHHHHCCCCEEHH
DAPTEVFPAALTYLGKNGASLDSADLKAAAEAVTKVRPSIKYFHSSKFINDLANGDICMA
CCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEE
HGYVGDLVQSRNRAAEAGKGVNIKIVIPKEGAVINIDSMVIPADAPHPENAHKFIDFMMK
CCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEECCEEECCCCCCCCHHHHHHHHHHH
QEVAAGFSNTTGYGNPIPGSRSLIRKEIQEDPVIFPSEQVTAKLYQVPPADMAKERERTR
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHEEECCCHHHHHHHHHHH
LWTTVKTGR
EEEEEECCC
>Mature Secondary Structure
MRISFTAVAVSLIAAAVSSVSGAQAAEDKVLNVYNWSDYIAPDTLEKFTALTGIKVNYDV
CEEEHHHHHHHHHHHHHHHCCCCHHHHCCEEEEECCCCCCCCHHHHHHHHHHCEEEEEEE
YDSNEVLQAKLQAGKSGYDVVFPSASPFLANQIKAGIYRKLDRSKLSNYGNLDAQVMVTL
ECCHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
NRAADPGNLYAIPYAISPTGFAYNVDKVAKAGKDMPLDSWAALFDPAQVAKLKGCGVSLL
ECCCCCCCEEEEEEEECCCCCEECHHHHHHCCCCCCCHHHHHHCCHHHHHHHCCCCEEHH
DAPTEVFPAALTYLGKNGASLDSADLKAAAEAVTKVRPSIKYFHSSKFINDLANGDICMA
CCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEE
HGYVGDLVQSRNRAAEAGKGVNIKIVIPKEGAVINIDSMVIPADAPHPENAHKFIDFMMK
CCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEECCEEECCCCCCCCHHHHHHHHHHH
QEVAAGFSNTTGYGNPIPGSRSLIRKEIQEDPVIFPSEQVTAKLYQVPPADMAKERERTR
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHEEECCCHHHHHHHHHHH
LWTTVKTGR
EEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]