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The map label for this gene is hsaB [H]

Identifier: 83311676

GI number: 83311676

Start: 2762900

End: 2763403

Strand: Reverse

Name: hsaB [H]

Synonym: amb2577

Alternate gene names: 83311676

Gene position: 2763403-2762900 (Counterclockwise)

Preceding gene: 83311683

Following gene: 83311674

Centisome position: 55.63

GC content: 62.3

Gene sequence:

>504_bases
ATGTCCCTTGATACCCGCTCTTTTCGCAAAACTCTGGGTTGCTTCGCCACTGGCGTTACGGTGGTCACGACCCTGATCCC
AGAGGCCAAGGAGCCGGTGGGCGTTACGGTCAGCGCGTTCTCGTCCCTGTCGCTGGAACCGCCGCTGGTGCTGTTCTGCC
TGGGCAACGGGACGTCCAGCATGGAGTCTTTCAAGACCTTTGGGCATTTTTGCGTCAACATTTTGTCGGAACATCAGCGG
GACCTCTCTATCCGCTTCGCCAGCCGGTCCGAGGACAAGTGGCAGGGCGTGTCGTGGAGTGCCGGCGCCAGCGGCGTACC
CGTCCTGGCCGGCTGCCTGGCCAGCCTGGAATGCTCGCTGGTCAACGTGGTTGATGGTGGCGACCATCAGATCTTCATCG
GCAAGGTGGAGAAGATGAAGCACCAGGAAGGCGGCAGTCCCCTGGTCTACTTCCGCGGCAGCTACATGGAGCCCGCGGCC
TCGCCCCTCACCGACGTGGTGTGA

Upstream 100 bases:

>100_bases
AAGTCGTCTCTTTTCACACACAATGGGAAAAGGCGCATTTGACCTGGACGACGGAACGCATTTAAATCTACATCCCGCGT
CCAATGATATCGAGCCGTTC

Downstream 100 bases:

>100_bases
CGGGCGGATTGCCTTGCAGACGGCGGGCGGTGACCTGCGTTACCGCCCCCGCTCCAGGGTCAGCACCGAACGCTTGGCGT
CTAGGCCCAGCCGGTAGAGG

Product: actinorhodin polyketide dimerase

Products: NAD(+); reduced flavin [C]

Alternate protein names: Flavin:NADH reductase [H]

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MSLDTRSFRKTLGCFATGVTVVTTLIPEAKEPVGVTVSAFSSLSLEPPLVLFCLGNGTSSMESFKTFGHFCVNILSEHQR
DLSIRFASRSEDKWQGVSWSAGASGVPVLAGCLASLECSLVNVVDGGDHQIFIGKVEKMKHQEGGSPLVYFRGSYMEPAA
SPLTDVV

Sequences:

>Translated_167_residues
MSLDTRSFRKTLGCFATGVTVVTTLIPEAKEPVGVTVSAFSSLSLEPPLVLFCLGNGTSSMESFKTFGHFCVNILSEHQR
DLSIRFASRSEDKWQGVSWSAGASGVPVLAGCLASLECSLVNVVDGGDHQIFIGKVEKMKHQEGGSPLVYFRGSYMEPAA
SPLTDVV
>Mature_166_residues
SLDTRSFRKTLGCFATGVTVVTTLIPEAKEPVGVTVSAFSSLSLEPPLVLFCLGNGTSSMESFKTFGHFCVNILSEHQRD
LSIRFASRSEDKWQGVSWSAGASGVPVLAGCLASLECSLVNVVDGGDHQIFIGKVEKMKHQEGGSPLVYFRGSYMEPAAS
PLTDVV

Specific function: Catalyzes the reduction of free flavins (FMN or FAD) by NADH. Subsequently, the reduced flavins diffuse to the HsaA oxygenase subunit [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=162, Percent_Identity=32.7160493827161, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: 1.6.8.- [C]

Molecular weight: Translated: 17850; Mature: 17718

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLDTRSFRKTLGCFATGVTVVTTLIPEAKEPVGVTVSAFSSLSLEPPLVLFCLGNGTSS
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHCCCCCCCCEEEEEECCCCCH
MESFKTFGHFCVNILSEHQRDLSIRFASRSEDKWQGVSWSAGASGVPVLAGCLASLECSL
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEE
VNVVDGGDHQIFIGKVEKMKHQEGGSPLVYFRGSYMEPAASPLTDVV
EEEEECCCCEEEEEHHHHHHHHCCCCEEEEECCCCCCCCCCCHHCCC
>Mature Secondary Structure 
SLDTRSFRKTLGCFATGVTVVTTLIPEAKEPVGVTVSAFSSLSLEPPLVLFCLGNGTSS
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHCCCCCCCCEEEEEECCCCCH
MESFKTFGHFCVNILSEHQRDLSIRFASRSEDKWQGVSWSAGASGVPVLAGCLASLECSL
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEE
VNVVDGGDHQIFIGKVEKMKHQEGGSPLVYFRGSYMEPAASPLTDVV
EEEEECCCCEEEEEHHHHHHHHCCCCEEEEECCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; flavin [C]

Specific reaction: NADH + flavin = NAD(+) + reduced flavin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]