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The map label for this gene is mepA

Identifier: 83311598

GI number: 83311598

Start: 2676266

End: 2677108

Strand: Reverse

Name: mepA

Synonym: amb2499

Alternate gene names: 83311598

Gene position: 2677108-2676266 (Counterclockwise)

Preceding gene: 83311599

Following gene: 83311597

Centisome position: 53.9

GC content: 70.82

Gene sequence:

>843_bases
ATGCATTCCCTGGCGCGTCTGATGGGGCTGGCCGCCCTGGTCCTGGCGACCGGCGCGGCGGCGGCCGAGTCCGGCCAGCA
GCAATGGTCGGGGATCGGCCAACCCGCCACGGGGCCGGCTTCGGTGATCGGCTCGCCCGCCGCCGGCTGTCTCAAGGGCG
CCGTGCCCTTGGCCGAGGACGAGCATTCGTTTCAGGTGCTGCGGCCGTCGCGCAACCGCCATTGGGGGCATCCAGCGACA
ATCCGCTTTGTGGGCGATCTCGCCAGGGCGACGAAGGGGGAGGGGATCTCGGGTCCGCTCCTGATCGGCGACATGGCGCA
GCCGCGGGGCGGCCCCATGCCCGCCGGTCACGGCAGCCATCAGAACGGTCTGGACGTTGACATCTGGTTCCGACTGCCGG
CCAAGCCCCTGAGCCGCGCCGAGATCGAGACGCCCAAGCCCGTCTCCATGGTTTACGGCACCGAGATCGACGAGGATGCC
TGGACTCCGGCCCAGGCCCGTCTGCTGGAACTGGCGGCCCGCGCCCCCCAGGTGGACCGCATCTTCGTCAACCCCGCCAT
CAAGAAGGCCATGTGCCGCGCCGTTCCCGCCGATGGCGACCGGTCGTGGCTGCGCAAGCTGCGTCCCTGGTGGGGGCATG
ACGAGCATTTCCACGTGCGCCTGTCGTGCCCCGCCGACAGCCCCGATTGCGAGCGGCAAAAGCCCATGCCCGAAGGCGAT
GGCTGCGGCGAGGAACTAGAGTCCTGGTCGGCCAGGCCCACCTGGCCCGCGCCGCCGTCCAGGCCCCATCACCAGTCCCG
CCCGCTGCCCGAAGCCTGCGCCGGCTTGTTCCGCAAGGGGTAA

Upstream 100 bases:

>100_bases
CCGGGGAAAAGTTCGATCCCCAGTGCGTTGCCGCTCTGATCGACAGCGCCGTTCAGATCGGTGATATTCAGACTCGCTTT
GACGAGACGGTGTACGACTG

Downstream 100 bases:

>100_bases
GCCATTACATCTCTTGCAATTGGCCGGGGAAGGGCGTAGAACCCCGCCACCAATCCGCACGCGGGATCGCGGCGACGGCA
TGTCTCTTGCCGTTCGTTCG

Product: penicillin-insensitive murein endopeptidase

Products: Split Peptidoglycan Strands [C]

Alternate protein names: D-alanyl-D-alanine-endopeptidase; DD-endopeptidase [H]

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MHSLARLMGLAALVLATGAAAAESGQQQWSGIGQPATGPASVIGSPAAGCLKGAVPLAEDEHSFQVLRPSRNRHWGHPAT
IRFVGDLARATKGEGISGPLLIGDMAQPRGGPMPAGHGSHQNGLDVDIWFRLPAKPLSRAEIETPKPVSMVYGTEIDEDA
WTPAQARLLELAARAPQVDRIFVNPAIKKAMCRAVPADGDRSWLRKLRPWWGHDEHFHVRLSCPADSPDCERQKPMPEGD
GCGEELESWSARPTWPAPPSRPHHQSRPLPEACAGLFRKG

Sequences:

>Translated_280_residues
MHSLARLMGLAALVLATGAAAAESGQQQWSGIGQPATGPASVIGSPAAGCLKGAVPLAEDEHSFQVLRPSRNRHWGHPAT
IRFVGDLARATKGEGISGPLLIGDMAQPRGGPMPAGHGSHQNGLDVDIWFRLPAKPLSRAEIETPKPVSMVYGTEIDEDA
WTPAQARLLELAARAPQVDRIFVNPAIKKAMCRAVPADGDRSWLRKLRPWWGHDEHFHVRLSCPADSPDCERQKPMPEGD
GCGEELESWSARPTWPAPPSRPHHQSRPLPEACAGLFRKG
>Mature_280_residues
MHSLARLMGLAALVLATGAAAAESGQQQWSGIGQPATGPASVIGSPAAGCLKGAVPLAEDEHSFQVLRPSRNRHWGHPAT
IRFVGDLARATKGEGISGPLLIGDMAQPRGGPMPAGHGSHQNGLDVDIWFRLPAKPLSRAEIETPKPVSMVYGTEIDEDA
WTPAQARLLELAARAPQVDRIFVNPAIKKAMCRAVPADGDRSWLRKLRPWWGHDEHFHVRLSCPADSPDCERQKPMPEGD
GCGEELESWSARPTWPAPPSRPHHQSRPLPEACAGLFRKG

Specific function: Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein [H]

COG id: COG3770

COG function: function code M; Murein endopeptidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M74 family [H]

Homologues:

Organism=Escherichia coli, GI1788668, Length=258, Percent_Identity=42.2480620155039, Blast_Score=197, Evalue=5e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR005073 [H]

Pfam domain/function: PF03411 Peptidase_M74 [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 30175; Mature: 30175

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSLARLMGLAALVLATGAAAAESGQQQWSGIGQPATGPASVIGSPAAGCLKGAVPLAED
CHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHCCCCCCCC
EHSFQVLRPSRNRHWGHPATIRFVGDLARATKGEGISGPLLIGDMAQPRGGPMPAGHGSH
CCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCC
QNGLDVDIWFRLPAKPLSRAEIETPKPVSMVYGTEIDEDAWTPAQARLLELAARAPQVDR
CCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCE
IFVNPAIKKAMCRAVPADGDRSWLRKLRPWWGHDEHFHVRLSCPADSPDCERQKPMPEGD
EEECHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHCCCCCCCC
GCGEELESWSARPTWPAPPSRPHHQSRPLPEACAGLFRKG
CCHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MHSLARLMGLAALVLATGAAAAESGQQQWSGIGQPATGPASVIGSPAAGCLKGAVPLAED
CHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHCCCCCCCC
EHSFQVLRPSRNRHWGHPATIRFVGDLARATKGEGISGPLLIGDMAQPRGGPMPAGHGSH
CCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCC
QNGLDVDIWFRLPAKPLSRAEIETPKPVSMVYGTEIDEDAWTPAQARLLELAARAPQVDR
CCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCE
IFVNPAIKKAMCRAVPADGDRSWLRKLRPWWGHDEHFHVRLSCPADSPDCERQKPMPEGD
EEECHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCEEEEEEECCCCCCCCHHCCCCCCCC
GCGEELESWSARPTWPAPPSRPHHQSRPLPEACAGLFRKG
CCHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond [C]

Specific reaction: Split The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond Connecting Neighboring Peptidoglycan Strands. [C]

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA