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The map label for this gene is tsf

Identifier: 83311596

GI number: 83311596

Start: 2674268

End: 2675206

Strand: Reverse

Name: tsf

Synonym: amb2497

Alternate gene names: 83311596

Gene position: 2675206-2674268 (Counterclockwise)

Preceding gene: 83311597

Following gene: 83311595

Centisome position: 53.86

GC content: 67.31

Gene sequence:

>939_bases
ATGGCCGAGATTACCGCTTCGCTGGTCAAGGAGCTGCGCGAGAAGACCGGCGCCGGCATGATGGACTGCAAGAAGGCGCT
GGGCGAGACCGCCGGTGACGTCGAAGCCGCCATCGACTGGCTGCGCAAGAAGGGCCTTGCCGCCGCCGCCAAGAAGGCTG
GCCGCGTTGCCGCCGAGGGTCTGGTGGGCATCGCCGCCGCCGGCACCAAGGGCGTGGCCGTCGAAGTCAATGCCGAGACC
GATTTCGTCGCCCGCAACGACCAGTTCCAGGGCTTTGTCGCCTCGGTCGCCGCCGTGGCGCTGGACAAGGGCGCCGACGT
GGAGGCCATCAAGGCCGCTGCCTGCCCCGGCACCGACAAGAACGTCGCTGACCAGCTGACCCACCTGATCGCCACCATCG
GCGAGAACATGTCGCTGCGTCGTGCCGTGCGCCTGGAAGTGTCGGCCGGCGTGGTCGCTTCCTATGTTCACACCGCCATC
GCTCCCGGCCTGGGCAAGATCGGCTGCCTGGTGGCTCTCGAGTCCACCGGCAACGTGGATCGCCTGAACGAAGTGGGCAA
GCAGATCGCCATGCACGTGGCCGCCGCCAACCCGCTGTTCCTGGACCCGTCGGTGGTCGACACCAGCGCTCTCGATCGCG
AGCGCAACGTGCTGACCGAGCAGGCCCAGGCTTCGGGCAAGCCCGCCGCCGTCATCGAGAAGATGGTCGAAGGCCGCATC
CGCAAGTACTACGAGGAAGTCTGCCTGTCCGAGCAGGTTTTCGTCATCGACCAGGAGAACAAGATCTCCAAGGTGCTCGA
GAACCTGGGCAAGGAGATCGGCGCGCCGGTCAAGCTGGCCGGTTTCGCCCGCTTCGCCCTGGGCGAGGGCATCGAGAAGG
AAGTCAGCGACTTCGCCGCCGAAGTGGCGGCTCAGGCTGGCACCCGCCCGGCCGGCTGA

Upstream 100 bases:

>100_bases
GCAGGCCTAGGCTTCGTCAGACTGTTGCGATGGCGGCGACCGGATGTCGCCGCCATTCGCTTGAGAACCAACCAATTCAT
TCGTCGAGAGGGGAATACCC

Downstream 100 bases:

>100_bases
CCTTCCGGTCCGCAATGAGGGCCGCTCCTTCCTGACGGAAGGGGCGGCCTTTTTTCTCTCCACAAGGGGGGAGACAGGCG
GGTGGAATGGTGTATGATCG

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MAEITASLVKELREKTGAGMMDCKKALGETAGDVEAAIDWLRKKGLAAAAKKAGRVAAEGLVGIAAAGTKGVAVEVNAET
DFVARNDQFQGFVASVAAVALDKGADVEAIKAAACPGTDKNVADQLTHLIATIGENMSLRRAVRLEVSAGVVASYVHTAI
APGLGKIGCLVALESTGNVDRLNEVGKQIAMHVAAANPLFLDPSVVDTSALDRERNVLTEQAQASGKPAAVIEKMVEGRI
RKYYEEVCLSEQVFVIDQENKISKVLENLGKEIGAPVKLAGFARFALGEGIEKEVSDFAAEVAAQAGTRPAG

Sequences:

>Translated_312_residues
MAEITASLVKELREKTGAGMMDCKKALGETAGDVEAAIDWLRKKGLAAAAKKAGRVAAEGLVGIAAAGTKGVAVEVNAET
DFVARNDQFQGFVASVAAVALDKGADVEAIKAAACPGTDKNVADQLTHLIATIGENMSLRRAVRLEVSAGVVASYVHTAI
APGLGKIGCLVALESTGNVDRLNEVGKQIAMHVAAANPLFLDPSVVDTSALDRERNVLTEQAQASGKPAAVIEKMVEGRI
RKYYEEVCLSEQVFVIDQENKISKVLENLGKEIGAPVKLAGFARFALGEGIEKEVSDFAAEVAAQAGTRPAG
>Mature_311_residues
AEITASLVKELREKTGAGMMDCKKALGETAGDVEAAIDWLRKKGLAAAAKKAGRVAAEGLVGIAAAGTKGVAVEVNAETD
FVARNDQFQGFVASVAAVALDKGADVEAIKAAACPGTDKNVADQLTHLIATIGENMSLRRAVRLEVSAGVVASYVHTAIA
PGLGKIGCLVALESTGNVDRLNEVGKQIAMHVAAANPLFLDPSVVDTSALDRERNVLTEQAQASGKPAAVIEKMVEGRIR
KYYEEVCLSEQVFVIDQENKISKVLENLGKEIGAPVKLAGFARFALGEGIEKEVSDFAAEVAAQAGTRPAG

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family

Homologues:

Organism=Homo sapiens, GI171846268, Length=218, Percent_Identity=38.9908256880734, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI291084500, Length=239, Percent_Identity=34.7280334728033, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI291084498, Length=94, Percent_Identity=47.8723404255319, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI291084502, Length=94, Percent_Identity=47.8723404255319, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1786366, Length=308, Percent_Identity=46.4285714285714, Blast_Score=225, Evalue=3e-60,
Organism=Caenorhabditis elegans, GI17561440, Length=212, Percent_Identity=31.1320754716981, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19921466, Length=304, Percent_Identity=29.6052631578947, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): EFTS_MAGSA (Q2W4C4)

Other databases:

- EMBL:   AP007255
- RefSeq:   YP_421860.1
- ProteinModelPortal:   Q2W4C4
- SMR:   Q2W4C4
- STRING:   Q2W4C4
- GeneID:   3803214
- GenomeReviews:   AP007255_GR
- KEGG:   mag:amb2497
- NMPDR:   fig|342108.5.peg.2197
- eggNOG:   COG0264
- HOGENOM:   HBG713289
- OMA:   YLHGTRI
- PhylomeDB:   Q2W4C4
- ProtClustDB:   PRK09377
- BioCyc:   MMAG342108:AMB2497-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00050
- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449
- Gene3D:   G3DSA:3.30.479.20
- PANTHER:   PTHR11741
- TIGRFAMs:   TIGR00116

Pfam domain/function: PF00889 EF_TS; PF00627 UBA; SSF54713 EF_TS; SSF46934 UBA_like

EC number: NA

Molecular weight: Translated: 32453; Mature: 32322

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS01126 EF_TS_1; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEITASLVKELREKTGAGMMDCKKALGETAGDVEAAIDWLRKKGLAAAAKKAGRVAAEG
CCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHH
LVGIAAAGTKGVAVEVNAETDFVARNDQFQGFVASVAAVALDKGADVEAIKAAACPGTDK
HHHEEECCCCCEEEEECCCCCEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCH
NVADQLTHLIATIGENMSLRRAVRLEVSAGVVASYVHTAIAPGLGKIGCLVALESTGNVD
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHH
RLNEVGKQIAMHVAAANPLFLDPSVVDTSALDRERNVLTEQAQASGKPAAVIEKMVEGRI
HHHHHHHHHHHHHHCCCCCEECCCHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
RKYYEEVCLSEQVFVIDQENKISKVLENLGKEIGAPVKLAGFARFALGEGIEKEVSDFAA
HHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHCCCEEHHHHHHHHHCCCHHHHHHHHHH
EVAAQAGTRPAG
HHHHHCCCCCCC
>Mature Secondary Structure 
AEITASLVKELREKTGAGMMDCKKALGETAGDVEAAIDWLRKKGLAAAAKKAGRVAAEG
CHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHH
LVGIAAAGTKGVAVEVNAETDFVARNDQFQGFVASVAAVALDKGADVEAIKAAACPGTDK
HHHEEECCCCCEEEEECCCCCEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCH
NVADQLTHLIATIGENMSLRRAVRLEVSAGVVASYVHTAIAPGLGKIGCLVALESTGNVD
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHH
RLNEVGKQIAMHVAAANPLFLDPSVVDTSALDRERNVLTEQAQASGKPAAVIEKMVEGRI
HHHHHHHHHHHHHHCCCCCEECCCHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
RKYYEEVCLSEQVFVIDQENKISKVLENLGKEIGAPVKLAGFARFALGEGIEKEVSDFAA
HHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHCCCEEHHHHHHHHHCCCHHHHHHHHHH
EVAAQAGTRPAG
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA