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The map label for this gene is lpxD

Identifier: 83311587

GI number: 83311587

Start: 2664897

End: 2665916

Strand: Reverse

Name: lpxD

Synonym: amb2488

Alternate gene names: 83311587

Gene position: 2665916-2664897 (Counterclockwise)

Preceding gene: 83311588

Following gene: 83311586

Centisome position: 53.67

GC content: 65.98

Gene sequence:

>1020_bases
ATGGCCGATCCCCGTTTCTTTCGTGTTGCCGGTCCTTTCACGCTTGCCCAACTGGCCGAGCTTTCGGGCGCGTCCGTGGC
GGAGGGCTGCGATCTCGGCGCCAGCTTCGTAGACGTGGCGCCGCTGGAACAGGCGGGCCAGGACAACGTTTCGTTTCTGG
ACAACCGCAAATATGTCGGTGCCTTCCAGGCCAGCAAGGCCGGGCTGTGCGTAATCGCCCCCGAGATGGCGGACAAGGCC
CCCGAGGGAATGGCGCTGCTGCTGTCGCCCGATCCTTATCGTGCCTATGCTCGCATCGCCCAGGCATTCTATCCCAATCC
GGCGCCCGAGCCCTGGGTGGCCCCCACGGCTTGGGTCGACGCCAGCGCCGCTGTGGGCGAGGGCTGCCGTATTGAACCGG
GGGCGGTGATCGGCGCCGGCGCCCGGATCGGAGCCCGCTGCCGTATCGGCGCCAATGTGGTGATCGGTCAAGGCGTGGTG
TTGGGCGACGATTGCACCATCGGCGCCAATGCCACCGTTTCCCACGCACTGGTCGGCAGCCGCGTCAATATTTATCCAGG
CGCCCGCATCGGCCAGGACGGGTTCGGGTTCGCCATGGGGCCGCAGGGTCACCTGAAGGTGCCGCAATTGGGTCGCGTGT
TGATCGGCAATAACGTGGAGATCGGCGCCAACACCACCATCGACCGGGGCGCTGGGCCGGACACGGTGATCGGTGATGGC
TCGATGATAGACAACCTCGTCCAGATTGGGCACAACGTACAACTGGGGCGCGGTTGCGTCATCGTGGCTCAGGTCGGAAT
TTCCGGATCCACCCGCATGGGTGACTTCGTCGCGGCGGGCGGACAGGCCGGCATCACCGGTCATCTGAAAATCGGTGCGG
GCGCCAAGATCGCCGCCCAGGCCGGCGTGATGCGCGACATCGCTCCGGGTGAGACTGTCGGCGGCGCGCCCGCCGTGCCC
ATGGCGGATTGGCTGCGCCAGTCGGCCATTCTGGGCAAGATGGCGCGAAAAAAGAGTTAG

Upstream 100 bases:

>100_bases
TTGAACAAGAAGCTGCCCACGGTCAATTTCCCCATTCCTCAGGTGGATGATCATTCCTCACCAGAGGTTCCGCCCTCCAA
GTCCGGCAAGAAGTGACACC

Downstream 100 bases:

>100_bases
GCCAAAGGCAGGACGTGAGTATGGATACCGCCAACAACGACCAGGGCACCGACCTGGGCAAGGTTATCGACATCAGCCGG
ATCATCCAGATGATTCCGCA

Product: UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase

Products: UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine; [Acyl-Carrier Protein]. [C]

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MADPRFFRVAGPFTLAQLAELSGASVAEGCDLGASFVDVAPLEQAGQDNVSFLDNRKYVGAFQASKAGLCVIAPEMADKA
PEGMALLLSPDPYRAYARIAQAFYPNPAPEPWVAPTAWVDASAAVGEGCRIEPGAVIGAGARIGARCRIGANVVIGQGVV
LGDDCTIGANATVSHALVGSRVNIYPGARIGQDGFGFAMGPQGHLKVPQLGRVLIGNNVEIGANTTIDRGAGPDTVIGDG
SMIDNLVQIGHNVQLGRGCVIVAQVGISGSTRMGDFVAAGGQAGITGHLKIGAGAKIAAQAGVMRDIAPGETVGGAPAVP
MADWLRQSAILGKMARKKS

Sequences:

>Translated_339_residues
MADPRFFRVAGPFTLAQLAELSGASVAEGCDLGASFVDVAPLEQAGQDNVSFLDNRKYVGAFQASKAGLCVIAPEMADKA
PEGMALLLSPDPYRAYARIAQAFYPNPAPEPWVAPTAWVDASAAVGEGCRIEPGAVIGAGARIGARCRIGANVVIGQGVV
LGDDCTIGANATVSHALVGSRVNIYPGARIGQDGFGFAMGPQGHLKVPQLGRVLIGNNVEIGANTTIDRGAGPDTVIGDG
SMIDNLVQIGHNVQLGRGCVIVAQVGISGSTRMGDFVAAGGQAGITGHLKIGAGAKIAAQAGVMRDIAPGETVGGAPAVP
MADWLRQSAILGKMARKKS
>Mature_338_residues
ADPRFFRVAGPFTLAQLAELSGASVAEGCDLGASFVDVAPLEQAGQDNVSFLDNRKYVGAFQASKAGLCVIAPEMADKAP
EGMALLLSPDPYRAYARIAQAFYPNPAPEPWVAPTAWVDASAAVGEGCRIEPGAVIGAGARIGARCRIGANVVIGQGVVL
GDDCTIGANATVSHALVGSRVNIYPGARIGQDGFGFAMGPQGHLKVPQLGRVLIGNNVEIGANTTIDRGAGPDTVIGDGS
MIDNLVQIGHNVQLGRGCVIVAQVGISGSTRMGDFVAAGGQAGITGHLKIGAGAKIAAQAGVMRDIAPGETVGGAPAVPM
ADWLRQSAILGKMARKKS

Specific function: Lipid A biosynthesis; third step. [C]

COG id: COG1044

COG function: function code M; UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family. LpxD subfamily

Homologues:

Organism=Escherichia coli, GI1786376, Length=295, Percent_Identity=36.271186440678, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1786378, Length=209, Percent_Identity=29.1866028708134, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LPXD_MAGSA (Q2W4D3)

Other databases:

- EMBL:   AP007255
- RefSeq:   YP_421851.1
- ProteinModelPortal:   Q2W4D3
- SMR:   Q2W4D3
- STRING:   Q2W4D3
- GeneID:   3804693
- GenomeReviews:   AP007255_GR
- KEGG:   mag:amb2488
- NMPDR:   fig|342108.5.peg.2189
- eggNOG:   COG1044
- HOGENOM:   HBG469615
- OMA:   IAQAFYP
- PhylomeDB:   Q2W4D3
- ProtClustDB:   PRK00892
- BioCyc:   MMAG342108:AMB2488-MONOMER
- HAMAP:   MF_00523
- InterPro:   IPR001451
- InterPro:   IPR011004
- InterPro:   IPR007691
- InterPro:   IPR020573
- TIGRFAMs:   TIGR01853

Pfam domain/function: PF00132 Hexapep; PF04613 LpxD; SSF51161 Trimer_LpxA_like

EC number: 2.3.1.- [C]

Molecular weight: Translated: 34449; Mature: 34318

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADPRFFRVAGPFTLAQLAELSGASVAEGCDLGASFVDVAPLEQAGQDNVSFLDNRKYVG
CCCCCEEEEECCHHHHHHHHCCCCCCCCCCCCCCCHHCCCCHHHCCCCCCEEECCCCEEE
AFQASKAGLCVIAPEMADKAPEGMALLLSPDPYRAYARIAQAFYPNPAPEPWVAPTAWVD
EEECCCCCEEEECHHHHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCHHHCC
ASAAVGEGCRIEPGAVIGAGARIGARCRIGANVVIGQGVVLGDDCTIGANATVSHALVGS
CCHHCCCCCEECCCCEEECCCCCCCEEEECCEEEEECCEEECCCCCCCCCCCHHHHHHCC
RVNIYPGARIGQDGFGFAMGPQGHLKVPQLGRVLIGNNVEIGANTTIDRGAGPDTVIGDG
EEEECCCCEECCCCCCEEECCCCCEECCCCCEEEECCCEEECCCCCCCCCCCCCCEECCH
SMIDNLVQIGHNVQLGRGCVIVAQVGISGSTRMGDFVAAGGQAGITGHLKIGAGAKIAAQ
HHHHHHHHHCCCEEECCCEEEEEEECCCCCCCCCCEEECCCCCCCEEEEEECCCCEEHHH
AGVMRDIAPGETVGGAPAVPMADWLRQSAILGKMARKKS
HCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ADPRFFRVAGPFTLAQLAELSGASVAEGCDLGASFVDVAPLEQAGQDNVSFLDNRKYVG
CCCCEEEEECCHHHHHHHHCCCCCCCCCCCCCCCHHCCCCHHHCCCCCCEEECCCCEEE
AFQASKAGLCVIAPEMADKAPEGMALLLSPDPYRAYARIAQAFYPNPAPEPWVAPTAWVD
EEECCCCCEEEECHHHHCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCHHHCC
ASAAVGEGCRIEPGAVIGAGARIGARCRIGANVVIGQGVVLGDDCTIGANATVSHALVGS
CCHHCCCCCEECCCCEEECCCCCCCEEEECCEEEEECCEEECCCCCCCCCCCHHHHHHCC
RVNIYPGARIGQDGFGFAMGPQGHLKVPQLGRVLIGNNVEIGANTTIDRGAGPDTVIGDG
EEEECCCCEECCCCCCEEECCCCCEECCCCCEEEECCCEEECCCCCCCCCCCCCCEECCH
SMIDNLVQIGHNVQLGRGCVIVAQVGISGSTRMGDFVAAGGQAGITGHLKIGAGAKIAAQ
HHHHHHHHHCCCEEECCCEEEEEEECCCCCCCCCCEEECCCCCCCEEEEEECCCCEEHHH
AGVMRDIAPGETVGGAPAVPMADWLRQSAILGKMARKKS
HCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine; (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] [C]

Specific reaction: UDP-3-O-(3-Hydroxytetradecanoyl)Glucosamine + (R)-3-Hydroxytetradecanoyl-[Acyl-Carrier Protein] = UDP-2,3-Bis(3-Hydroxytetradecanoyl)Glucosamine + [Acyl-Carrier Protein]. [C]

General reaction: Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA