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The map label for this gene is ispDF

Identifier: 83311462

GI number: 83311462

Start: 2566090

End: 2567244

Strand: Reverse

Name: ispDF

Synonym: amb2363

Alternate gene names: 83311462

Gene position: 2567244-2566090 (Counterclockwise)

Preceding gene: 83311474

Following gene: 83311458

Centisome position: 51.68

GC content: 69.61

Gene sequence:

>1155_bases
ATGGCCAAGACGGTTGTGCTGGTGGTGGCGGCGGGACGCGGGCGGCGGTTCGGCGGCGACCTGCCCAAGCAGTATCACGA
TCTGGCCGGGCGCATGGTGTTGCGCCACACCCTGGCGGCCTTCGCCACCAATCCCGAGATCGGTGCCGTGCGCGCCGTCA
TCCATCCCGACGACCGCCAGCTCTACGACATGGCCGCCGCCGGCCTGAACCTGCTGGAGCCGGTCCATGGCGGCGCCACC
CGCCAGGACAGCGTGCGCCTGGGCCTCGACAGCCTGAAGGATCTCAATCCGGACAAGGTGCTAATCCATGATGGGGCGCG
GCCCTTCATCGATCACGGCACCATCGGCCGGGTGATCCGCGCCCTTGACCGCCACCCCGGCGCCCTGCCCGTGGTTCCCG
TCGCCGATACCCTGAAGCGCGGCCAGGACGGTTTCGTGGCCGACACGGTGGACCGCGCCAATCTGTTCCGCGCCCAAACT
CCCCAGGGATTCCGCTATGCCGAGATCGTCGCCGCCCATCACGCGGTGATCGGCAACGAGCTGACCGACGATGCCGCCGT
GGCGGAAAAGGCCGGGCTGGCGGTGGAACTGGTGAACGGCGCCGAGGACAACGTCAAGATCACCACCGGCGCCGATCTGG
AGCGGGCGCGCTGCCTGTTCGACGGCCCCGGCGAGGTCCGCTCCGCCTCGGGCTACGACGTGCACCGCTTCGACCCGGCC
AAGGATGCCTGCTGGCTGTGCGGCGTTCCCGTGCCGCACGAGGCCGGGCTGCTGGGCCATTCCGACGCTGATGTCGGGCT
GCACGCCCTGACCGATGCCGTGCTGGGCGCCATCTCGGCCGGCGACATCGGCCATCATTTCCCGCCCACCGATGCCAGGT
GGAAGGGAGCCGCCTCGGACCAGTTTCTGGCCCATGCCGGCTCGCTGGTCACCGCCAAGGGCGGCCGCATCGTCAATGTG
GACGTGACCATCATCTGCGAGCGCCCCAAGGTGGGGCCGCACCGCGCCGCCATGGCGGCGAGAGTGGCGGAAATCCTTGG
CATTTCCCAGGACCGGGTCAGCGTCAAGGCCACCACCACCGAGGGATTGGGCTTTACCGGCCGCAAGGAGGGCATCGCCG
CCCAGGCCATGGCCTCGGTCTGGCTGCCGCGATAA

Upstream 100 bases:

>100_bases
CCCTTGGGCCTAGCCGGCGCACAGAGGCGCCGGTATCAAGCCTTGGCAGGAGGCATCGGCTCGCATAGTCTGTCGCCCGC
TTTTCCAAGGGAGATCGGAT

Downstream 100 bases:

>100_bases
ATCAGCCGCCGGTCTCGGTCCTGATCCAGTTGAGATAGGCGGGATTGCCCGCCTCGATGGGTAAGGCCACCACGCAGGGG
CAGGCGTAGGAGTGCAACTC

Product: IspD/ispF bifunctional enzyme

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MAKTVVLVVAAGRGRRFGGDLPKQYHDLAGRMVLRHTLAAFATNPEIGAVRAVIHPDDRQLYDMAAAGLNLLEPVHGGAT
RQDSVRLGLDSLKDLNPDKVLIHDGARPFIDHGTIGRVIRALDRHPGALPVVPVADTLKRGQDGFVADTVDRANLFRAQT
PQGFRYAEIVAAHHAVIGNELTDDAAVAEKAGLAVELVNGAEDNVKITTGADLERARCLFDGPGEVRSASGYDVHRFDPA
KDACWLCGVPVPHEAGLLGHSDADVGLHALTDAVLGAISAGDIGHHFPPTDARWKGAASDQFLAHAGSLVTAKGGRIVNV
DVTIICERPKVGPHRAAMAARVAEILGISQDRVSVKATTTEGLGFTGRKEGIAAQAMASVWLPR

Sequences:

>Translated_384_residues
MAKTVVLVVAAGRGRRFGGDLPKQYHDLAGRMVLRHTLAAFATNPEIGAVRAVIHPDDRQLYDMAAAGLNLLEPVHGGAT
RQDSVRLGLDSLKDLNPDKVLIHDGARPFIDHGTIGRVIRALDRHPGALPVVPVADTLKRGQDGFVADTVDRANLFRAQT
PQGFRYAEIVAAHHAVIGNELTDDAAVAEKAGLAVELVNGAEDNVKITTGADLERARCLFDGPGEVRSASGYDVHRFDPA
KDACWLCGVPVPHEAGLLGHSDADVGLHALTDAVLGAISAGDIGHHFPPTDARWKGAASDQFLAHAGSLVTAKGGRIVNV
DVTIICERPKVGPHRAAMAARVAEILGISQDRVSVKATTTEGLGFTGRKEGIAAQAMASVWLPR
>Mature_383_residues
AKTVVLVVAAGRGRRFGGDLPKQYHDLAGRMVLRHTLAAFATNPEIGAVRAVIHPDDRQLYDMAAAGLNLLEPVHGGATR
QDSVRLGLDSLKDLNPDKVLIHDGARPFIDHGTIGRVIRALDRHPGALPVVPVADTLKRGQDGFVADTVDRANLFRAQTP
QGFRYAEIVAAHHAVIGNELTDDAAVAEKAGLAVELVNGAEDNVKITTGADLERARCLFDGPGEVRSASGYDVHRFDPAK
DACWLCGVPVPHEAGLLGHSDADVGLHALTDAVLGAISAGDIGHHFPPTDARWKGAASDQFLAHAGSLVTAKGGRIVNVD
VTIICERPKVGPHRAAMAARVAEILGISQDRVSVKATTTEGLGFTGRKEGIAAQAMASVWLPR

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=153, Percent_Identity=53.5947712418301, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1789104, Length=215, Percent_Identity=33.953488372093, Blast_Score=101, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPDF_MAGSA (Q2W4Q8)

Other databases:

- EMBL:   AP007255
- RefSeq:   YP_421726.1
- HSSP:   P62617
- ProteinModelPortal:   Q2W4Q8
- SMR:   Q2W4Q8
- STRING:   Q2W4Q8
- GeneID:   3803940
- GenomeReviews:   AP007255_GR
- KEGG:   mag:amb2363
- NMPDR:   fig|342108.5.peg.2083
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   IVLIHDA
- PhylomeDB:   Q2W4Q8
- ProtClustDB:   CLSK943350
- BioCyc:   MMAG342108:AMB2363-MONOMER
- HAMAP:   MF_01520
- InterPro:   IPR001228
- InterPro:   IPR018294
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00453
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF01128 IspD; PF02542 YgbB; SSF69765 YgbB_synth

EC number: =2.7.7.60; =4.6.1.12

Molecular weight: Translated: 40555; Mature: 40423

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS01295 ISPD; PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTVVLVVAAGRGRRFGGDLPKQYHDLAGRMVLRHTLAAFATNPEIGAVRAVIHPDDRQ
CCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHH
LYDMAAAGLNLLEPVHGGATRQDSVRLGLDSLKDLNPDKVLIHDGARPFIDHGTIGRVIR
HHHHHHHCHHHHHHCCCCCCCCCHHEECHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHH
ALDRHPGALPVVPVADTLKRGQDGFVADTVDRANLFRAQTPQGFRYAEIVAAHHAVIGNE
HHHCCCCCCEEEECHHHHHCCCCCCEECCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
LTDDAAVAEKAGLAVELVNGAEDNVKITTGADLERARCLFDGPGEVRSASGYDVHRFDPA
CCCHHHHHHHCCCEEEEECCCCCCEEEEECCCHHHCEEEECCCCCCCCCCCCCEECCCCC
KDACWLCGVPVPHEAGLLGHSDADVGLHALTDAVLGAISAGDIGHHFPPTDARWKGAASD
CCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
QFLAHAGSLVTAKGGRIVNVDVTIICERPKVGPHRAAMAARVAEILGISQDRVSVKATTT
HHHHHCCCEEEECCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEC
EGLGFTGRKEGIAAQAMASVWLPR
CCCCCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
AKTVVLVVAAGRGRRFGGDLPKQYHDLAGRMVLRHTLAAFATNPEIGAVRAVIHPDDRQ
CCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHH
LYDMAAAGLNLLEPVHGGATRQDSVRLGLDSLKDLNPDKVLIHDGARPFIDHGTIGRVIR
HHHHHHHCHHHHHHCCCCCCCCCHHEECHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHH
ALDRHPGALPVVPVADTLKRGQDGFVADTVDRANLFRAQTPQGFRYAEIVAAHHAVIGNE
HHHCCCCCCEEEECHHHHHCCCCCCEECCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCC
LTDDAAVAEKAGLAVELVNGAEDNVKITTGADLERARCLFDGPGEVRSASGYDVHRFDPA
CCCHHHHHHHCCCEEEEECCCCCCEEEEECCCHHHCEEEECCCCCCCCCCCCCEECCCCC
KDACWLCGVPVPHEAGLLGHSDADVGLHALTDAVLGAISAGDIGHHFPPTDARWKGAASD
CCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
QFLAHAGSLVTAKGGRIVNVDVTIICERPKVGPHRAAMAARVAEILGISQDRVSVKATTT
HHHHHCCCEEEECCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEC
EGLGFTGRKEGIAAQAMASVWLPR
CCCCCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA