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The map label for this gene is dctB [H]

Identifier: 83311381

GI number: 83311381

Start: 2482787

End: 2484115

Strand: Reverse

Name: dctB [H]

Synonym: amb2282

Alternate gene names: 83311381

Gene position: 2484115-2482787 (Counterclockwise)

Preceding gene: 83311383

Following gene: 83311380

Centisome position: 50.01

GC content: 66.89

Gene sequence:

>1329_bases
ATGGCCCAGCTCACCGACCCCCGCCCCTCTTCGGCCTATGATTTCGCTGGCGGCTATGATCCCGACATGGCGGCGGCCCA
GGAGACCGCCTGGATCGAAGTGATCCGCAAGATGGAGGAGGTCTATTCCGACCTCATCTCCTACGAGGTGGAGCTGGAGG
AGAAGAACACCGCCCTGGAAGAAGCCCAGCGCTTCATCAATTCCGTGCTGTCGGCGGTGTCGGACATCCTGATCGTCTGC
GACCAGAACGGCCGCATCCAGCAGGTCAACCTGGCCTTCCTCCAGTTGACCGGCCTAGCCGAGGTGGACCTGTTCGAATG
CCCGCTGGAGGATTTGCTGGCCGAGGATACCGGCCAGTTGCGCCTGTTCAGCTGGATCGACGCCGTCGAGGGCCATGACC
GCGAGGTGCGCTTCCGCTCGGCCGATGGCGGGCTGACCGACCCTGTGGCGCTGTCTTGCGCCACGCGGCTGGATCATCGC
GGCCTGCCCGCCGGCATCGTGCTGACCGGGCGGCCGGTGGGCGAGCTTCGCCGTGCCTACGAGGCGCTGAAGACCGCCCA
GGCCCAGTTGATCCAGCAGGAAAAGATGGCCTCCCTCGGCCGCCTGATCGCCGGGGTGGCCCACGAACTCAACAACCCCA
TCAGCTTCGTCTACGGCAATGTCCACGCCCTGTCCAAATACTCAACGCGCATCGCCGCCTATCTCGACGCCATCCACGCC
GGTTGCGACGAGACCGAGCGCGAGGCCCTGCGGGCCAGCCTGCGCATCGATTCGACCCTGGCCGACCTGCCCGCCCTGAT
GGAGGGCACCGCCGAGGGGGCCCAGCGGGTGGCCGACATCGTGCGCAGCCTGAAGCGCCTGTCCTTCTCGGCCGGATCCA
AGCCCGAGGAGTTCGACCTGGGCGAAGTGGTGGCCAAGGCGGTGCAATGGTCTGGATCGGGCAAGAAGGGACTAGCCAGC
ATCGAACTGGACCTGCCCGATCATCTGGCGGTGCGCGGCAATCCCGGCCAGTTGCACCAGGTAATCGTCAACCTGATCGA
GAATGCCCTGGACGCCGTGGCGGGCCGCCCCGACGGCCGCGTCGAGGTGACTGGCAAGGCCGAGGGCGGCCAAACCGTGC
TGACCATCGCCGATAACGGCCCCGGCATTCCCCCCGACATCGTCGGCAAGGTGTTCGACCCCTTCTTCACCACCAAGCCG
GTGGGCAAGGGAACCGGCCTGGGTCTGTGGATCTCCTACGGCATCATCCGCGACCATGGAGGGTCGCTGGAGGCCGGCGC
CACCGCCTCGGGGGGCGCCCGCTTCACCATCAGCCTGCCACCGGCTTGA

Upstream 100 bases:

>100_bases
CACGTTTCGGCCACCCTGACCAGGGACGGACAAAGGGCATTGATCCCGGCTCTCTCGAGGAACACACTGCGCCTGACAAC
ACGCAGGCGGTTCAAAACGC

Downstream 100 bases:

>100_bases
GCCCGTCGCCCACTCCCTCTATGCTGCCGCCCATGACCCGAGAAAAACCCGCCATCACCCTTTGCGCCGACGATTACGGG
CTGGCCCCGGGGCTCGGCAT

Product: uptake hydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MAQLTDPRPSSAYDFAGGYDPDMAAAQETAWIEVIRKMEEVYSDLISYEVELEEKNTALEEAQRFINSVLSAVSDILIVC
DQNGRIQQVNLAFLQLTGLAEVDLFECPLEDLLAEDTGQLRLFSWIDAVEGHDREVRFRSADGGLTDPVALSCATRLDHR
GLPAGIVLTGRPVGELRRAYEALKTAQAQLIQQEKMASLGRLIAGVAHELNNPISFVYGNVHALSKYSTRIAAYLDAIHA
GCDETEREALRASLRIDSTLADLPALMEGTAEGAQRVADIVRSLKRLSFSAGSKPEEFDLGEVVAKAVQWSGSGKKGLAS
IELDLPDHLAVRGNPGQLHQVIVNLIENALDAVAGRPDGRVEVTGKAEGGQTVLTIADNGPGIPPDIVGKVFDPFFTTKP
VGKGTGLGLWISYGIIRDHGGSLEAGATASGGARFTISLPPA

Sequences:

>Translated_442_residues
MAQLTDPRPSSAYDFAGGYDPDMAAAQETAWIEVIRKMEEVYSDLISYEVELEEKNTALEEAQRFINSVLSAVSDILIVC
DQNGRIQQVNLAFLQLTGLAEVDLFECPLEDLLAEDTGQLRLFSWIDAVEGHDREVRFRSADGGLTDPVALSCATRLDHR
GLPAGIVLTGRPVGELRRAYEALKTAQAQLIQQEKMASLGRLIAGVAHELNNPISFVYGNVHALSKYSTRIAAYLDAIHA
GCDETEREALRASLRIDSTLADLPALMEGTAEGAQRVADIVRSLKRLSFSAGSKPEEFDLGEVVAKAVQWSGSGKKGLAS
IELDLPDHLAVRGNPGQLHQVIVNLIENALDAVAGRPDGRVEVTGKAEGGQTVLTIADNGPGIPPDIVGKVFDPFFTTKP
VGKGTGLGLWISYGIIRDHGGSLEAGATASGGARFTISLPPA
>Mature_441_residues
AQLTDPRPSSAYDFAGGYDPDMAAAQETAWIEVIRKMEEVYSDLISYEVELEEKNTALEEAQRFINSVLSAVSDILIVCD
QNGRIQQVNLAFLQLTGLAEVDLFECPLEDLLAEDTGQLRLFSWIDAVEGHDREVRFRSADGGLTDPVALSCATRLDHRG
LPAGIVLTGRPVGELRRAYEALKTAQAQLIQQEKMASLGRLIAGVAHELNNPISFVYGNVHALSKYSTRIAAYLDAIHAG
CDETEREALRASLRIDSTLADLPALMEGTAEGAQRVADIVRSLKRLSFSAGSKPEEFDLGEVVAKAVQWSGSGKKGLASI
ELDLPDHLAVRGNPGQLHQVIVNLIENALDAVAGRPDGRVEVTGKAEGGQTVLTIADNGPGIPPDIVGKVFDPFFTTKPV
GKGTGLGLWISYGIIRDHGGSLEAGATASGGARFTISLPPA

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. DctB functions as a membrane-associated protein kinase that phosphorylates dctD in response to environmental signals [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=389, Percent_Identity=27.7634961439589, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1790436, Length=267, Percent_Identity=29.9625468164794, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1786912, Length=258, Percent_Identity=28.6821705426357, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI87081816, Length=117, Percent_Identity=35.042735042735, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788393, Length=253, Percent_Identity=26.0869565217391, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI145693157, Length=275, Percent_Identity=25.0909090909091, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1786600, Length=245, Percent_Identity=24.8979591836735, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR017055
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 47324; Mature: 47193

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQLTDPRPSSAYDFAGGYDPDMAAAQETAWIEVIRKMEEVYSDLISYEVELEEKNTALE
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECHHHHHHH
EAQRFINSVLSAVSDILIVCDQNGRIQQVNLAFLQLTGLAEVDLFECPLEDLLAEDTGQL
HHHHHHHHHHHHHHHEEEEECCCCCEEEEEEEEEEECCCCCCHHHHCCHHHHHHCCCCCE
RLFSWIDAVEGHDREVRFRSADGGLTDPVALSCATRLDHRGLPAGIVLTGRPVGELRRAY
EHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHH
EALKTAQAQLIQQEKMASLGRLIAGVAHELNNPISFVYGNVHALSKYSTRIAAYLDAIHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHC
GCDETEREALRASLRIDSTLADLPALMEGTAEGAQRVADIVRSLKRLSFSAGSKPEEFDL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
GEVVAKAVQWSGSGKKGLASIELDLPDHLAVRGNPGQLHQVIVNLIENALDAVAGRPDGR
HHHHHHHHHCCCCCCCCCEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCE
VEVTGKAEGGQTVLTIADNGPGIPPDIVGKVFDPFFTTKPVGKGTGLGLWISYGIIRDHG
EEEEEECCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEECCC
GSLEAGATASGGARFTISLPPA
CCCCCCCCCCCCCEEEEECCCC
>Mature Secondary Structure 
AQLTDPRPSSAYDFAGGYDPDMAAAQETAWIEVIRKMEEVYSDLISYEVELEEKNTALE
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECHHHHHHH
EAQRFINSVLSAVSDILIVCDQNGRIQQVNLAFLQLTGLAEVDLFECPLEDLLAEDTGQL
HHHHHHHHHHHHHHHEEEEECCCCCEEEEEEEEEEECCCCCCHHHHCCHHHHHHCCCCCE
RLFSWIDAVEGHDREVRFRSADGGLTDPVALSCATRLDHRGLPAGIVLTGRPVGELRRAY
EHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHH
EALKTAQAQLIQQEKMASLGRLIAGVAHELNNPISFVYGNVHALSKYSTRIAAYLDAIHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHC
GCDETEREALRASLRIDSTLADLPALMEGTAEGAQRVADIVRSLKRLSFSAGSKPEEFDL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
GEVVAKAVQWSGSGKKGLASIELDLPDHLAVRGNPGQLHQVIVNLIENALDAVAGRPDGR
HHHHHHHHHCCCCCCCCCEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCE
VEVTGKAEGGQTVLTIADNGPGIPPDIVGKVFDPFFTTKPVGKGTGLGLWISYGIIRDHG
EEEEEECCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEECCC
GSLEAGATASGGARFTISLPPA
CCCCCCCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3671068 [H]