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The map label for this gene is hyb0 [C]

Identifier: 83311042

GI number: 83311042

Start: 2087838

End: 2088863

Strand: Reverse

Name: hyb0 [C]

Synonym: amb1943

Alternate gene names: 83311042

Gene position: 2088863-2087838 (Counterclockwise)

Preceding gene: 83311043

Following gene: 83311041

Centisome position: 42.05

GC content: 69.88

Gene sequence:

>1026_bases
ATGGTCGCGCCGTCCCCTCCCCCCGACACCTATAACGTCCTGTGGCTGCAGGCGGCCAGTTGCGGCGGCTGCACCATGGC
AGCCCTGGCGGCCGATGATACCGGGCTGCTGCGCTCGATGGCGCGGTTCGGCATCCGGCTGCTGTGGCATCCCTCGCTGT
CCGAGGAAAGCGGCGCCGAGGCGCTCGCCGTGCTAGAGGACTGCGCCACGGGCAAGCTGCGCCTGGACTGCCTGTGCGTC
GAGGGATCGGTGTTACTGGGCCCCAATGGCAGCGGGCGCTTCCAGATGCTGTCGGGCAGCGGCGCCCCCATGAAGGACTG
GATCGCCCGATTGGCCCCCAAGGCCCGGCACGTGGTGGCGGTGGGAAGCTGCGCCGCCTTCGGCGGCATGCCGGCCGCCG
GACACAACCCCACCGACGCGCGCGGCCTGCAGTATTCCGGCTGGGACCCGGGCGGCGCGCTGGGCAAGGCCTTCCGCGCC
GGTTCGGGCCTGCCGGTGGTCAACATCGCCGGCTGCGCCCCCCATCCGGGCTGGATGATCGAGACCCTGGCGGCGCTGGC
ATTGGGCGATGTCTCGGCCGAGCATCTGGACGCCTTGGGCCGTCCGCGCGCCTATGCCGACCATCTGGCCCATCATGGTT
GCGGGCGCAACGAGTTCTATGAGTTCAAGGCCAGCGCGGCACGGCCGTCCGATCGCGGCTGTCTAATGGAAAACCTCGGC
TGTCGCGCCACCCAGGCGCCAGGCGATTGCAATATCCGCCGCTGGAACGGCTATGGCTCGTGCACCGATGGCGGCTATGC
CTGCATCAACTGCACATCCCCCGCCTTCGAGACGCCGTCGGGTCCCTTCCAGGAGACCGCCAAGGTGGCCGGCATCCCCG
TGGGGCTGCCGGTGGACATGCCCAAGGCATGGTTCGTCGCCCTGGCCGCCTTGTCCAAATCGGCGACGCCGCAGCGGGTG
CGCACCAATGCCCATGCCGACCACGTCATCGTCCCACCCGCCCGCAAGCCCCTGAAGTCGCCATGA

Upstream 100 bases:

>100_bases
GCTTCGGCTGGCGCACCCCGGCCGCCCGCGCCCTGATCGGCCGCGACCAGCCACCGGGAGAGGCTTGAACCTGCCTCCCT
CTAGCCTCTAATGTGGCGGC

Downstream 100 bases:

>100_bases
CCCTAAGCTCCACCCGCCTGATCGTCGGCCCCTTCAACCGGGTCGAGGGCGACCTGGAAGTGACCCTGGACGTCCAGGAC
GGACGGGTGGCCGAAGCCCG

Product: uptake hydrogenase

Products: NA

Alternate protein names: NiFeSe hydrogenlyase small chain [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MVAPSPPPDTYNVLWLQAASCGGCTMAALAADDTGLLRSMARFGIRLLWHPSLSEESGAEALAVLEDCATGKLRLDCLCV
EGSVLLGPNGSGRFQMLSGSGAPMKDWIARLAPKARHVVAVGSCAAFGGMPAAGHNPTDARGLQYSGWDPGGALGKAFRA
GSGLPVVNIAGCAPHPGWMIETLAALALGDVSAEHLDALGRPRAYADHLAHHGCGRNEFYEFKASAARPSDRGCLMENLG
CRATQAPGDCNIRRWNGYGSCTDGGYACINCTSPAFETPSGPFQETAKVAGIPVGLPVDMPKAWFVALAALSKSATPQRV
RTNAHADHVIVPPARKPLKSP

Sequences:

>Translated_341_residues
MVAPSPPPDTYNVLWLQAASCGGCTMAALAADDTGLLRSMARFGIRLLWHPSLSEESGAEALAVLEDCATGKLRLDCLCV
EGSVLLGPNGSGRFQMLSGSGAPMKDWIARLAPKARHVVAVGSCAAFGGMPAAGHNPTDARGLQYSGWDPGGALGKAFRA
GSGLPVVNIAGCAPHPGWMIETLAALALGDVSAEHLDALGRPRAYADHLAHHGCGRNEFYEFKASAARPSDRGCLMENLG
CRATQAPGDCNIRRWNGYGSCTDGGYACINCTSPAFETPSGPFQETAKVAGIPVGLPVDMPKAWFVALAALSKSATPQRV
RTNAHADHVIVPPARKPLKSP
>Mature_341_residues
MVAPSPPPDTYNVLWLQAASCGGCTMAALAADDTGLLRSMARFGIRLLWHPSLSEESGAEALAVLEDCATGKLRLDCLCV
EGSVLLGPNGSGRFQMLSGSGAPMKDWIARLAPKARHVVAVGSCAAFGGMPAAGHNPTDARGLQYSGWDPGGALGKAFRA
GSGLPVVNIAGCAPHPGWMIETLAALALGDVSAEHLDALGRPRAYADHLAHHGCGRNEFYEFKASAARPSDRGCLMENLG
CRATQAPGDCNIRRWNGYGSCTDGGYACINCTSPAFETPSGPFQETAKVAGIPVGLPVDMPKAWFVALAALSKSATPQRV
RTNAHADHVIVPPARKPLKSP

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd2 Is Involved In Hydrogen Uptake. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789371, Length=291, Percent_Identity=28.1786941580756, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1787206, Length=272, Percent_Identity=26.1029411764706, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 35509; Mature: 35509

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: PS00436 PEROXIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAPSPPPDTYNVLWLQAASCGGCTMAALAADDTGLLRSMARFGIRLLWHPSLSEESGAE
CCCCCCCCCCEEEEEEEECCCCCCEEEEEECCCHHHHHHHHHHCEEEEECCCCCCCCCHH
ALAVLEDCATGKLRLDCLCVEGSVLLGPNGSGRFQMLSGSGAPMKDWIARLAPKARHVVA
HHHHHHHCCCCCEEEEEEEECCCEEECCCCCCCEEEEECCCCCHHHHHHHHCCCCCEEEE
VGSCAAFGGMPAAGHNPTDARGLQYSGWDPGGALGKAFRAGSGLPVVNIAGCAPHPGWMI
EECHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCHHH
ETLAALALGDVSAEHLDALGRPRAYADHLAHHGCGRNEFYEFKASAARPSDRGCLMENLG
HHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHCCCCCCHHEEEHHCCCCCCCCCHHHHHCC
CRATQAPGDCNIRRWNGYGSCTDGGYACINCTSPAFETPSGPFQETAKVAGIPVGLPVDM
CCCCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCCC
PKAWFVALAALSKSATPQRVRTNAHADHVIVPPARKPLKSP
CHHHHHHHHHHHCCCCHHHHHCCCCCCEEEECCCCCCCCCC
>Mature Secondary Structure
MVAPSPPPDTYNVLWLQAASCGGCTMAALAADDTGLLRSMARFGIRLLWHPSLSEESGAE
CCCCCCCCCCEEEEEEEECCCCCCEEEEEECCCHHHHHHHHHHCEEEEECCCCCCCCCHH
ALAVLEDCATGKLRLDCLCVEGSVLLGPNGSGRFQMLSGSGAPMKDWIARLAPKARHVVA
HHHHHHHCCCCCEEEEEEEECCCEEECCCCCCCEEEEECCCCCHHHHHHHHCCCCCEEEE
VGSCAAFGGMPAAGHNPTDARGLQYSGWDPGGALGKAFRAGSGLPVVNIAGCAPHPGWMI
EECHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCHHH
ETLAALALGDVSAEHLDALGRPRAYADHLAHHGCGRNEFYEFKASAARPSDRGCLMENLG
HHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHCCCCCCHHEEEHHCCCCCCCCCHHHHHCC
CRATQAPGDCNIRRWNGYGSCTDGGYACINCTSPAFETPSGPFQETAKVAGIPVGLPVDM
CCCCCCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCCC
PKAWFVALAALSKSATPQRVRTNAHADHVIVPPARKPLKSP
CHHHHHHHHHHHCCCCHHHHHCCCCCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3316183; 3322275; 10378275 [H]