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The map label for this gene is hybC [C]

Identifier: 83311041

GI number: 83311041

Start: 2086396

End: 2087841

Strand: Reverse

Name: hybC [C]

Synonym: amb1942

Alternate gene names: 83311041

Gene position: 2087841-2086396 (Counterclockwise)

Preceding gene: 83311042

Following gene: 83311030

Centisome position: 42.03

GC content: 70.4

Gene sequence:

>1446_bases
ATGACCCTAAGCTCCACCCGCCTGATCGTCGGCCCCTTCAACCGGGTCGAGGGCGACCTGGAAGTGACCCTGGACGTCCA
GGACGGACGGGTGGCCGAAGCCCGGGTCAACGCCCCCCTCTATCGCGGCTTCGAGCAGATCCTGCTGGGACGTCCGCCCT
CGGACGCCCTGGTGATCGCCCCCAGGATCTGCGGCATCTGCTCGGTATCGCAATCGGTGGCCTCGGCCCGCGCCCTGGCG
GGTTTGGGTCCCGCCGCCATGCCGGAAAATGGCACCCTGGCCACCAATCTCATCCTGGCCTGCGAGAACGCTGCCGATCA
CCTCACCCATTTCATGCTGTTCTTCGCCCCCGATCTGGCGCGAAGCTGCTATGGCGGACGGCCGTGGTTCGCCGCGGCCA
GGCGGCGTTTCGCGGCGGGCGATGGCGAATGCGCCCGAGCCCTGCTGCCGGCCCGAGCCCGCTTCCTGCATCTGATGGGC
CTGCTGGCGGGCAAGTGGCCGCACTCGCTGGCGCTGCAGCCCGGCGGGGTCACCAAAGCCGTCGATCCGGGCGAGAAGAT
CCGCCTGCTCTCCATCCTGGCCGAGTTCCGCGAGGCGCTGGAGACCCATCTGTTCGCCGACAGCCTGGAAGCCATCGCCG
CCCTCGACGGACTCCCCGCGCTCGATGCCTGGGCCGATGCCGCCCCGCCCGAGGCCGGCGATTTCCGCCTGTTCCTGCAC
ATCGCCCGCGACCTGGACTTCACCGGATTGGGCGCCGGCCCCGGCCGCTTCATCTCGTCGGGAAGCTTCGGCCTGTTCGC
CCCCGGGGTGTGGGCCCAAGGCGCGTCGACGCCCTTCGACCCGGCCCAGGTCAGCGAGGATCATGCCCACTCGTGGATGG
AGGGCGCAATCCTCGCGCCGGCCGTCGGCAAAACCATTCCCGCCCCCGATAAGCCCGAAGGCTATAGCTGGTGCAAAGCG
CCCCGCCTGGACGGACAGGTGGCCGAGGTGGGAGCCTTGGCCCGTCAGGTGGTAGACGGCCACCCCCTGGCCCGCGCCCT
CAACGCCGCCCGCCCCGGCGGCTCGGTGCACGACCGGGTGGTGGCCCGGCTGCTGGAACTGGCCCGGCTGATCCCGGCCA
TGGAAAGCTGGATCCGCGCCATCCGCCCGGCCGAGCCGTTTTGCGCCCCCCCCTCCCCCCTCGGCGAAGGCGCCGCCGTC
GGCCTGGTGGAGGCCGCCCGCGGGACCCTGGGACATTGGCTTGGCCTGCACAACGGCCGCATCGCCCAATACCAGATTGT
CGCCCCGACCACCTGGAACTTTTCCCCTCGCGATGCCCAAGGCAATCCAGGAGCCCTCGAAATCGCGCTAGCCGGAGCCC
CCGTCCTGCCCGGCGAAACCACCCCAGTCGCCGTCCAGCACGTCGTCCGCTCCTTTGACCCCTGCATGGTCTGCACGGTG
CATTGA

Upstream 100 bases:

>100_bases
TGGCCGCCTTGTCCAAATCGGCGACGCCGCAGCGGGTGCGCACCAATGCCCATGCCGACCACGTCATCGTCCCACCCGCC
CGCAAGCCCCTGAAGTCGCC

Downstream 100 bases:

>100_bases
AATTTTATCGTTTAATATCAATGTGTTGCCGAACCGCGTCCCGAATGCGTCCCGAAATCGTCCCTAATCAGCCCGGAACA
CGCACCCAATCGTCGCCCCG

Product: uptake hydrogenase

Products: NA

Alternate protein names: NiFeSe hydrogenlyase large chain [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MTLSSTRLIVGPFNRVEGDLEVTLDVQDGRVAEARVNAPLYRGFEQILLGRPPSDALVIAPRICGICSVSQSVASARALA
GLGPAAMPENGTLATNLILACENAADHLTHFMLFFAPDLARSCYGGRPWFAAARRRFAAGDGECARALLPARARFLHLMG
LLAGKWPHSLALQPGGVTKAVDPGEKIRLLSILAEFREALETHLFADSLEAIAALDGLPALDAWADAAPPEAGDFRLFLH
IARDLDFTGLGAGPGRFISSGSFGLFAPGVWAQGASTPFDPAQVSEDHAHSWMEGAILAPAVGKTIPAPDKPEGYSWCKA
PRLDGQVAEVGALARQVVDGHPLARALNAARPGGSVHDRVVARLLELARLIPAMESWIRAIRPAEPFCAPPSPLGEGAAV
GLVEAARGTLGHWLGLHNGRIAQYQIVAPTTWNFSPRDAQGNPGALEIALAGAPVLPGETTPVAVQHVVRSFDPCMVCTV
H

Sequences:

>Translated_481_residues
MTLSSTRLIVGPFNRVEGDLEVTLDVQDGRVAEARVNAPLYRGFEQILLGRPPSDALVIAPRICGICSVSQSVASARALA
GLGPAAMPENGTLATNLILACENAADHLTHFMLFFAPDLARSCYGGRPWFAAARRRFAAGDGECARALLPARARFLHLMG
LLAGKWPHSLALQPGGVTKAVDPGEKIRLLSILAEFREALETHLFADSLEAIAALDGLPALDAWADAAPPEAGDFRLFLH
IARDLDFTGLGAGPGRFISSGSFGLFAPGVWAQGASTPFDPAQVSEDHAHSWMEGAILAPAVGKTIPAPDKPEGYSWCKA
PRLDGQVAEVGALARQVVDGHPLARALNAARPGGSVHDRVVARLLELARLIPAMESWIRAIRPAEPFCAPPSPLGEGAAV
GLVEAARGTLGHWLGLHNGRIAQYQIVAPTTWNFSPRDAQGNPGALEIALAGAPVLPGETTPVAVQHVVRSFDPCMVCTV
H
>Mature_480_residues
TLSSTRLIVGPFNRVEGDLEVTLDVQDGRVAEARVNAPLYRGFEQILLGRPPSDALVIAPRICGICSVSQSVASARALAG
LGPAAMPENGTLATNLILACENAADHLTHFMLFFAPDLARSCYGGRPWFAAARRRFAAGDGECARALLPARARFLHLMGL
LAGKWPHSLALQPGGVTKAVDPGEKIRLLSILAEFREALETHLFADSLEAIAALDGLPALDAWADAAPPEAGDFRLFLHI
ARDLDFTGLGAGPGRFISSGSFGLFAPGVWAQGASTPFDPAQVSEDHAHSWMEGAILAPAVGKTIPAPDKPEGYSWCKAP
RLDGQVAEVGALARQVVDGHPLARALNAARPGGSVHDRVVARLLELARLIPAMESWIRAIRPAEPFCAPPSPLGEGAAVG
LVEAARGTLGHWLGLHNGRIAQYQIVAPTTWNFSPRDAQGNPGALEIALAGAPVLPGETTPVAVQHVVRSFDPCMVCTVH

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd2 Is Involved In Hydrogen Uptake. [C]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789368, Length=563, Percent_Identity=27.708703374778, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1787207, Length=579, Percent_Identity=26.0794473229706, Blast_Score=150, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 50726; Mature: 50594

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00507 NI_HGENASE_L_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSSTRLIVGPFNRVEGDLEVTLDVQDGRVAEARVNAPLYRGFEQILLGRPPSDALVIA
CCCCCCEEEECCHHHCCCCEEEEEEECCCEEEHHHCCCHHHHHHHHHHCCCCCCCCEEEC
PRICGICSVSQSVASARALAGLGPAAMPENGTLATNLILACENAADHLTHFMLFFAPDLA
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHH
RSCYGGRPWFAAARRRFAAGDGECARALLPARARFLHLMGLLAGKWPHSLALQPGGVTKA
HHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCC
VDPGEKIRLLSILAEFREALETHLFADSLEAIAALDGLPALDAWADAAPPEAGDFRLFLH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCEEEEEE
IARDLDFTGLGAGPGRFISSGSFGLFAPGVWAQGASTPFDPAQVSEDHAHSWMEGAILAP
EHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHCCCEECC
AVGKTIPAPDKPEGYSWCKAPRLDGQVAEVGALARQVVDGHPLARALNAARPGGSVHDRV
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHH
VARLLELARLIPAMESWIRAIRPAEPFCAPPSPLGEGAAVGLVEAARGTLGHWLGLHNGR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
IAQYQIVAPTTWNFSPRDAQGNPGALEIALAGAPVLPGETTPVAVQHVVRSFDPCMVCTV
EEEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEEE
H
C
>Mature Secondary Structure 
TLSSTRLIVGPFNRVEGDLEVTLDVQDGRVAEARVNAPLYRGFEQILLGRPPSDALVIA
CCCCCEEEECCHHHCCCCEEEEEEECCCEEEHHHCCCHHHHHHHHHHCCCCCCCCEEEC
PRICGICSVSQSVASARALAGLGPAAMPENGTLATNLILACENAADHLTHFMLFFAPDLA
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHH
RSCYGGRPWFAAARRRFAAGDGECARALLPARARFLHLMGLLAGKWPHSLALQPGGVTKA
HHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCC
VDPGEKIRLLSILAEFREALETHLFADSLEAIAALDGLPALDAWADAAPPEAGDFRLFLH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCEEEEEE
IARDLDFTGLGAGPGRFISSGSFGLFAPGVWAQGASTPFDPAQVSEDHAHSWMEGAILAP
EHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHCCCEECC
AVGKTIPAPDKPEGYSWCKAPRLDGQVAEVGALARQVVDGHPLARALNAARPGGSVHDRV
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHH
VARLLELARLIPAMESWIRAIRPAEPFCAPPSPLGEGAAVGLVEAARGTLGHWLGLHNGR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
IAQYQIVAPTTWNFSPRDAQGNPGALEIALAGAPVLPGETTPVAVQHVVRSFDPCMVCTV
EEEEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEEE
H
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3316183; 10378275 [H]