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The map label for this gene is bepF [H]

Identifier: 83310975

GI number: 83310975

Start: 2023306

End: 2024532

Strand: Reverse

Name: bepF [H]

Synonym: amb1876

Alternate gene names: 83310975

Gene position: 2024532-2023306 (Counterclockwise)

Preceding gene: 83310976

Following gene: 83310974

Centisome position: 40.76

GC content: 69.11

Gene sequence:

>1227_bases
ATGACCGCTTCCCCCCGCTTCGCCAAGGCCCCCATCTTCAGCAAAGGCCCCGTCTTCAGCAAAGGTAGGGCCCTCCTTCT
CGCCACCGTCGCCCTGGCCGCCCTGGGTGGCGGCGCGGTGCTGCTCCGCGGCGCTCCCGGCCAGCCGGCCCAGGCCGCCG
CCGCCCAGGCGGCTCCCGTCACCGTGCAGACCATGGCGCTCCGCAACGTCCAGGTCTGGTCCTCGTTCTCGGGCCGCATG
CGGGCGGTGGACTTCGCCGAGATCCGGCCCGAGGTCAGCGGCCGCCTCACCCAGGTGCGCATCAATGACGGCCAGACGGT
CAAGGCCGGCGATGTGCTGTTCGTGATCGACCCCGCCCCCTTCGAGGCGGCGCTGGCCAAGGCCGAGGCCAATCTGGCCA
CCGCCCGGACCAACGCCGTCTTCGCCCGCACCGAACTTGACCGGGCCGTCAACCTGATCAAGACCGAGGCCATCGCCCAG
CGCCTCTATGACGAGCGCGCCAATGCCGACAAGGTGTCCCATTCCGCGGTGCTGGCCGCCGAGGCTGAACTGAAGCAGGC
GCACATCAACCTGGATCATGCCTATGTCAAGGCGCCCATCGCCGGCCGGGTCAGCCGCGCCGAAATCACCCTGGGCAACG
TGGTGCAGGCCGGCCCCGGCGCGCCGCTGCTGACCTCCATCGTCTCCAATGACGGCATCTACGCCGATTTCGAGGTGGAC
GAGCAGACCTACATGAAGGGCATCCGCACCCAGGACGGCTCCCGCGACAAGGAACGCCGCATCCCGGTGCAGATCACCGT
GCGCGGCGACGAGGCCCACCCCTATCTCGGCACCATTCAGAGCTTCGACAACCGCATCGACACCGCATCGGGGACCATCC
GCGCCCGGGCCCGCTTCGACAACGGGGACGGCGCCCTGATGCCGGGCATGTTCGTCTCGGTCAAGGTGGCCAGCGGCGGC
GACGAGCCGGCCCTGCTGGTGCAGGAACGCGCCATCGGCAGCGACCAGAGCAAGAAGTTCGTCTATGTGGTCGGCGACGA
CGGCAAGGTGGCCTATCGCGAGGTCGGGCTGGGGCCGCAGGTGGACGGAAGCCGCATCGTCCTGGCCGGACTCAAGGCCG
GCGAGAAGGTGATCGTCGACGGGCTGCAGCACGTCCGCCCCAACATGGCGGTCACCGTTCAGGAGGCCTCGCTGGGCACC
CTCAGACACGATATCGCCGCCAACTAG

Upstream 100 bases:

>100_bases
AAGGGCGGTATTTTTTGGATCATTGACTAGACTGACCGGTCAAATACTAGGGTATTATGCCCCCTGGCCTGCCGGTCATA
GACATCGAAGGAGCTTTCCC

Downstream 100 bases:

>100_bases
GCGCCGCATCCTCCTTTTCCGCTTTTGCCGCCCTCGCCCTTGAGAAGGCCACGCCATGACTTTGTCCAAATTCTTCATCG
ACCGCCCCATCTTCGCCGGG

Product: membrane-fusion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MTASPRFAKAPIFSKGPVFSKGRALLLATVALAALGGGAVLLRGAPGQPAQAAAAQAAPVTVQTMALRNVQVWSSFSGRM
RAVDFAEIRPEVSGRLTQVRINDGQTVKAGDVLFVIDPAPFEAALAKAEANLATARTNAVFARTELDRAVNLIKTEAIAQ
RLYDERANADKVSHSAVLAAEAELKQAHINLDHAYVKAPIAGRVSRAEITLGNVVQAGPGAPLLTSIVSNDGIYADFEVD
EQTYMKGIRTQDGSRDKERRIPVQITVRGDEAHPYLGTIQSFDNRIDTASGTIRARARFDNGDGALMPGMFVSVKVASGG
DEPALLVQERAIGSDQSKKFVYVVGDDGKVAYREVGLGPQVDGSRIVLAGLKAGEKVIVDGLQHVRPNMAVTVQEASLGT
LRHDIAAN

Sequences:

>Translated_408_residues
MTASPRFAKAPIFSKGPVFSKGRALLLATVALAALGGGAVLLRGAPGQPAQAAAAQAAPVTVQTMALRNVQVWSSFSGRM
RAVDFAEIRPEVSGRLTQVRINDGQTVKAGDVLFVIDPAPFEAALAKAEANLATARTNAVFARTELDRAVNLIKTEAIAQ
RLYDERANADKVSHSAVLAAEAELKQAHINLDHAYVKAPIAGRVSRAEITLGNVVQAGPGAPLLTSIVSNDGIYADFEVD
EQTYMKGIRTQDGSRDKERRIPVQITVRGDEAHPYLGTIQSFDNRIDTASGTIRARARFDNGDGALMPGMFVSVKVASGG
DEPALLVQERAIGSDQSKKFVYVVGDDGKVAYREVGLGPQVDGSRIVLAGLKAGEKVIVDGLQHVRPNMAVTVQEASLGT
LRHDIAAN
>Mature_407_residues
TASPRFAKAPIFSKGPVFSKGRALLLATVALAALGGGAVLLRGAPGQPAQAAAAQAAPVTVQTMALRNVQVWSSFSGRMR
AVDFAEIRPEVSGRLTQVRINDGQTVKAGDVLFVIDPAPFEAALAKAEANLATARTNAVFARTELDRAVNLIKTEAIAQR
LYDERANADKVSHSAVLAAEAELKQAHINLDHAYVKAPIAGRVSRAEITLGNVVQAGPGAPLLTSIVSNDGIYADFEVDE
QTYMKGIRTQDGSRDKERRIPVQITVRGDEAHPYLGTIQSFDNRIDTASGTIRARARFDNGDGALMPGMFVSVKVASGGD
EPALLVQERAIGSDQSKKFVYVVGDDGKVAYREVGLGPQVDGSRIVLAGLKAGEKVIVDGLQHVRPNMAVTVQEASLGTL
RHDIAAN

Specific function: May contribute to resistance to some drugs, such as deoxycholate, sodium dodecyl sulfate and nalidixic acid, in the absence of BepD and BepE [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=352, Percent_Identity=32.3863636363636, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1786668, Length=372, Percent_Identity=31.1827956989247, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1789665, Length=372, Percent_Identity=30.9139784946237, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI87082036, Length=368, Percent_Identity=29.3478260869565, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI87081951, Length=193, Percent_Identity=33.6787564766839, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI87081796, Length=342, Percent_Identity=25.7309941520468, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1789637, Length=189, Percent_Identity=28.042328042328, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790519, Length=252, Percent_Identity=29.3650793650794, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 43194; Mature: 43062

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASPRFAKAPIFSKGPVFSKGRALLLATVALAALGGGAVLLRGAPGQPAQAAAAQAAPV
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHCCCCE
TVQTMALRNVQVWSSFSGRMRAVDFAEIRPEVSGRLTQVRINDGQTVKAGDVLFVIDPAP
EEEEEEECCEEEECCCCCCEEEEEHHHHCCCCCCCEEEEEECCCCEEEECCEEEEECCCC
FEAALAKAEANLATARTNAVFARTELDRAVNLIKTEAIAQRLYDERANADKVSHSAVLAA
HHHHHHHHHCCCEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCEEEEE
EAELKQAHINLDHAYVKAPIAGRVSRAEITLGNVVQAGPGAPLLTSIVSNDGIYADFEVD
HHHHHHHHCCCCCEEEECCCCCCCEEEEEEECCEEECCCCCHHHHHHHCCCCEEEEEECC
EQTYMKGIRTQDGSRDKERRIPVQITVRGDEAHPYLGTIQSFDNRIDTASGTIRARARFD
HHHHHHCCCCCCCCCCHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCEEEEEEEEE
NGDGALMPGMFVSVKVASGGDEPALLVQERAIGSDQSKKFVYVVGDDGKVAYREVGLGPQ
CCCCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCEEEEEECCCCCEEEEEECCCCC
VDGSRIVLAGLKAGEKVIVDGLQHVRPNMAVTVQEASLGTLRHDIAAN
CCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCC
>Mature Secondary Structure 
TASPRFAKAPIFSKGPVFSKGRALLLATVALAALGGGAVLLRGAPGQPAQAAAAQAAPV
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHCCCCE
TVQTMALRNVQVWSSFSGRMRAVDFAEIRPEVSGRLTQVRINDGQTVKAGDVLFVIDPAP
EEEEEEECCEEEECCCCCCEEEEEHHHHCCCCCCCEEEEEECCCCEEEECCEEEEECCCC
FEAALAKAEANLATARTNAVFARTELDRAVNLIKTEAIAQRLYDERANADKVSHSAVLAA
HHHHHHHHHCCCEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCEEEEE
EAELKQAHINLDHAYVKAPIAGRVSRAEITLGNVVQAGPGAPLLTSIVSNDGIYADFEVD
HHHHHHHHCCCCCEEEECCCCCCCEEEEEEECCEEECCCCCHHHHHHHCCCCEEEEEECC
EQTYMKGIRTQDGSRDKERRIPVQITVRGDEAHPYLGTIQSFDNRIDTASGTIRARARFD
HHHHHHCCCCCCCCCCHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCEEEEEEEEE
NGDGALMPGMFVSVKVASGGDEPALLVQERAIGSDQSKKFVYVVGDDGKVAYREVGLGPQ
CCCCCCCCCEEEEEEEECCCCCCEEEEEECCCCCCCCCEEEEEECCCCCEEEEEECCCCC
VDGSRIVLAGLKAGEKVIVDGLQHVRPNMAVTVQEASLGTLRHDIAAN
CCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12271122 [H]