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The map label for this gene is braE [H]

Identifier: 83310903

GI number: 83310903

Start: 1943065

End: 1944312

Strand: Reverse

Name: braE [H]

Synonym: amb1804

Alternate gene names: 83310903

Gene position: 1944312-1943065 (Counterclockwise)

Preceding gene: 83310904

Following gene: 83310902

Centisome position: 39.14

GC content: 68.43

Gene sequence:

>1248_bases
ATGGGGGGCAAGACCGCCCGCGCCCTGGTGGTGGATGCCCTGCTGGGCGGAGTGGTGGCCGCCGCCATGGCCCTGCCCAT
GCTGGGCGTGCGGCTGGAGGACGGCTCCACCGGCCTGTCCCTGAGCGGGCGCCTCGACTGGGTGGCCTGGGCCGCCGGCA
GCGTGGCGGGCGCCCGGTTGCTGATCGGCCTCCTTCGCCTGGGACTGGCCGGACGCGGTCCCAGCCTTCCCGCCCCGCCG
CGCGCCATGATGGTCTATGTGGGCTGGGCCATGGTGGCCTTTGCCCTGATCCTGCCCTTCCTGCCCTTCTCGGGGCGCAA
TCTGGTGGACAAGGCCACCCTGGTGCTCATCTACGTCATGCTGGGCTGGGGCCTGAACATCGTGGTGGGGCTGGCCGGGC
TGCTGGATCTGGGCTTCGTCGCCTTCTACGCGGTGGGGGCCTATTCCTACGCCCTGCTCAGCCAGACCTTCGGGCTGTCC
TTCTGGGTCTGCCTGCCGCTGGCCGGTCTGCTGGCCGCGGCCTTCGGCATGGTGCTGGGCTTTCCGGTGCTGCGCCTGCG
CGGCGACTACATCGCCATCGTCACCATGGGATTGGGCGAGATCGTCCGGGTGGTGCTGCAGAACTGGCAAGACGTCACCG
GCGGCCCCAACGGCATTTCCGGCATCGAGCGGCCTTCGCTGTTCGGCCTGTCCTTCAAGATGGTACCGCCCGAGGGAAGC
CAGACCTTCGCCGAGTTCTTCGGCCTGGACTATTCCGCCGACCACCGGGTGATCTTCCTGTATTTCCTGATCCTGGCCTT
GGCGCTGCTGACCAACGTCATCACGTTGCGCATCCGCCGCCTGCCGGTGGGCCGGGCCTGGGAGGCCCTGCGCGAGGACG
AGATCGCCTGCCGATCCTTGGGCATCAACCCCACCCTGGTGAAGCTCTCGGCCTTCGCCACGGGGGCCATGTTCGCCGGA
TTCGCCGGGTCGTTCTTCGCCACCCGCCAGGGCTTCATCAGCCCGGAAAGCTTCACCTTCATCGAATCGGCGGTGATCCT
GGCCATCGTGGTGCTGGGCGGCATGGGCAGCCAGATCGGCATCGTGCTGGCGGCCCTGCTGCTGGTGGGCCTGCCGGAAT
GGTTCCGCGAGCTGCAGCAGTTCCGCATGCTGGCCTTCGGCGGCGCCATGGTGCTGATCATGCTGTGGAAGCCCGCCGGC
TTGCTGTCGACGCGGGAACCCACCATCCGGCTGGGAGAGGCGAAATGA

Upstream 100 bases:

>100_bases
ATCGAATACAAGGATGTGGCCACCTTCAGCATCCTGGTGGCGGTCCTGGTGTTCCGCCCCACCGGCTTGCTGGGCCGCCC
CGACGTGGAGAAGATCTGAC

Downstream 100 bases:

>100_bases
GCGGCCCCATCCCAACCTGTCATCCCGAGCCCCAGCGAGGGATCTCCGCCTGGCATGGCTTTGTCATGCCGGATCGGGCG
GATCAGGACGAGATCCCTCC

Product: ABC-type branched-chain amino acid transport system, permease component

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MGGKTARALVVDALLGGVVAAAMALPMLGVRLEDGSTGLSLSGRLDWVAWAAGSVAGARLLIGLLRLGLAGRGPSLPAPP
RAMMVYVGWAMVAFALILPFLPFSGRNLVDKATLVLIYVMLGWGLNIVVGLAGLLDLGFVAFYAVGAYSYALLSQTFGLS
FWVCLPLAGLLAAAFGMVLGFPVLRLRGDYIAIVTMGLGEIVRVVLQNWQDVTGGPNGISGIERPSLFGLSFKMVPPEGS
QTFAEFFGLDYSADHRVIFLYFLILALALLTNVITLRIRRLPVGRAWEALREDEIACRSLGINPTLVKLSAFATGAMFAG
FAGSFFATRQGFISPESFTFIESAVILAIVVLGGMGSQIGIVLAALLLVGLPEWFRELQQFRMLAFGGAMVLIMLWKPAG
LLSTREPTIRLGEAK

Sequences:

>Translated_415_residues
MGGKTARALVVDALLGGVVAAAMALPMLGVRLEDGSTGLSLSGRLDWVAWAAGSVAGARLLIGLLRLGLAGRGPSLPAPP
RAMMVYVGWAMVAFALILPFLPFSGRNLVDKATLVLIYVMLGWGLNIVVGLAGLLDLGFVAFYAVGAYSYALLSQTFGLS
FWVCLPLAGLLAAAFGMVLGFPVLRLRGDYIAIVTMGLGEIVRVVLQNWQDVTGGPNGISGIERPSLFGLSFKMVPPEGS
QTFAEFFGLDYSADHRVIFLYFLILALALLTNVITLRIRRLPVGRAWEALREDEIACRSLGINPTLVKLSAFATGAMFAG
FAGSFFATRQGFISPESFTFIESAVILAIVVLGGMGSQIGIVLAALLLVGLPEWFRELQQFRMLAFGGAMVLIMLWKPAG
LLSTREPTIRLGEAK
>Mature_414_residues
GGKTARALVVDALLGGVVAAAMALPMLGVRLEDGSTGLSLSGRLDWVAWAAGSVAGARLLIGLLRLGLAGRGPSLPAPPR
AMMVYVGWAMVAFALILPFLPFSGRNLVDKATLVLIYVMLGWGLNIVVGLAGLLDLGFVAFYAVGAYSYALLSQTFGLSF
WVCLPLAGLLAAAFGMVLGFPVLRLRGDYIAIVTMGLGEIVRVVLQNWQDVTGGPNGISGIERPSLFGLSFKMVPPEGSQ
TFAEFFGLDYSADHRVIFLYFLILALALLTNVITLRIRRLPVGRAWEALREDEIACRSLGINPTLVKLSAFATGAMFAGF
AGSFFATRQGFISPESFTFIESAVILAIVVLGGMGSQIGIVLAALLLVGLPEWFRELQQFRMLAFGGAMVLIMLWKPAGL
LSTREPTIRLGEAK

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=313, Percent_Identity=60.3833865814696, Blast_Score=358, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 44191; Mature: 44060

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGKTARALVVDALLGGVVAAAMALPMLGVRLEDGSTGLSLSGRLDWVAWAAGSVAGARL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCEECCCCCCHHHHHCHHHHHHH
LIGLLRLGLAGRGPSLPAPPRAMMVYVGWAMVAFALILPFLPFSGRNLVDKATLVLIYVM
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
LGWGLNIVVGLAGLLDLGFVAFYAVGAYSYALLSQTFGLSFWVCLPLAGLLAAAFGMVLG
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHC
FPVLRLRGDYIAIVTMGLGEIVRVVLQNWQDVTGGPNGISGIERPSLFGLSFKMVPPEGS
CHHHHCCCCEEEEEECCHHHHHHHHHHCCHHCCCCCCCCCCCCCCCEECEEEEECCCCCH
QTFAEFFGLDYSADHRVIFLYFLILALALLTNVITLRIRRLPVGRAWEALREDEIACRSL
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
GINPTLVKLSAFATGAMFAGFAGSFFATRQGFISPESFTFIESAVILAIVVLGGMGSQIG
CCCCHHEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHH
IVLAALLLVGLPEWFRELQQFRMLAFGGAMVLIMLWKPAGLLSTREPTIRLGEAK
HHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCEECCCCC
>Mature Secondary Structure 
GGKTARALVVDALLGGVVAAAMALPMLGVRLEDGSTGLSLSGRLDWVAWAAGSVAGARL
CCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCEECCCCCCHHHHHCHHHHHHH
LIGLLRLGLAGRGPSLPAPPRAMMVYVGWAMVAFALILPFLPFSGRNLVDKATLVLIYVM
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
LGWGLNIVVGLAGLLDLGFVAFYAVGAYSYALLSQTFGLSFWVCLPLAGLLAAAFGMVLG
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHC
FPVLRLRGDYIAIVTMGLGEIVRVVLQNWQDVTGGPNGISGIERPSLFGLSFKMVPPEGS
CHHHHCCCCEEEEEECCHHHHHHHHHHCCHHCCCCCCCCCCCCCCCEECEEEEECCCCCH
QTFAEFFGLDYSADHRVIFLYFLILALALLTNVITLRIRRLPVGRAWEALREDEIACRSL
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
GINPTLVKLSAFATGAMFAGFAGSFFATRQGFISPESFTFIESAVILAIVVLGGMGSQIG
CCCCHHEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHH
IVLAALLLVGLPEWFRELQQFRMLAFGGAMVLIMLWKPAGLLSTREPTIRLGEAK
HHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]