BacMap

Definition	Magnetospirillum magneticum AMB-1 chromosome, complete genome.
Accession	NC_007626
Length	4,967,148

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The map label for this gene is braF [H]

Identifier: 83310902

GI number: 83310902

Start: 1942113

End: 1942925

Strand: Reverse

Name: braF [H]

Synonym: amb1803

Alternate gene names: 83310902

Gene position: 1942925-1942113 (Counterclockwise)

Preceding gene: 83310903

Following gene: 83310901

Centisome position: 39.12

GC content: 66.42

Gene sequence:

>813_bases
ATGAGCAGCGAAGCCCTGCTCACCGTCGAACATGTGACCATGCGCTTCGGCGGCCTGTTCGCCGTCAACGATCTGTCGTT
CTCGGCTGCCCCTAAGGAGATCACGGCGGTGATCGGCCCCAATGGCGCCGGCAAGACCACGCTGTTCAACTGCATCACCG
GCTTTTACAAGCCGCAGGTGGGGCGCATGACGCTGGACCATCCGTCCGGCCCCATGCTGCTGGAGCGTCTGGACGACTTC
GCCATTTCCGCCAAGGCCGGGGTGGCGCGCACCTTCCAGAACATCCGCCTGTTCGCCCGCATGAGCGTGCTGGAGAACCT
GATCGTTGCCCAGCATACCACGCTGATGCGGGCCTCGGCGTTCTCCCTGGCCGGGTTGCTGGGTCTGTCGCGCTATGCCA
GGGCCGAGGCAAGGGCGGTGGAGCGGGCGCGGCACTGGCTGGACAAGGTGGGCCTGACCTCCCTGGCCGACGAGGAGGCG
GGCAGCCTGCCCTATGGCCATCAGCGCCGCCTGGAGATCGCCCGGGCCATGTGCACCGGGCCGCTTTTGCTGTGCCTGGA
CGAGCCCGCCGCCGGGTTGAACCCCCGCGAATCGGCGGAGCTGAACCGCCTGCTGCTCGACATCCGTGACGAAAACGGCA
TCGGGTTGCTGCTGATCGAACACGATATGAGCGTGGTCATGGAGATCTCGGACCATATCGTGGTGCTGGATTACGGCAAG
AAGATCGCCGAGGGCGCCCCCGCCGCCATCAAGGCCGATCCGGCGGTGATCCGCGCCTATCTGGGCGAGCCCGACGAAGA
GGAGGCCCGCTGA

Upstream 100 bases:

>100_bases
GGATCTCCGCCTGGCATGGCTTTGTCATGCCGGATCGGGCGGATCAGGACGAGATCCCTCCTCCCGATGGTCGTCGGGAT
GACACGACAGGGGGCATGGC

Downstream 100 bases:

>100_bases
TGCTGCGCATCGAAAGCCTTCACTCCGGCTATGGCCGCATCCAGGCGCTGCACGGTGTCGACATCAATGTGGCCGAGGGC
GAGATCGTCGCCCTGGTGGG

Product: ABC-type branched-chain amino acid transport systems, ATPase component

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MSSEALLTVEHVTMRFGGLFAVNDLSFSAAPKEITAVIGPNGAGKTTLFNCITGFYKPQVGRMTLDHPSGPMLLERLDDF
AISAKAGVARTFQNIRLFARMSVLENLIVAQHTTLMRASAFSLAGLLGLSRYARAEARAVERARHWLDKVGLTSLADEEA
GSLPYGHQRRLEIARAMCTGPLLLCLDEPAAGLNPRESAELNRLLLDIRDENGIGLLLIEHDMSVVMEISDHIVVLDYGK
KIAEGAPAAIKADPAVIRAYLGEPDEEEAR

Sequences:

>Translated_270_residues
MSSEALLTVEHVTMRFGGLFAVNDLSFSAAPKEITAVIGPNGAGKTTLFNCITGFYKPQVGRMTLDHPSGPMLLERLDDF
AISAKAGVARTFQNIRLFARMSVLENLIVAQHTTLMRASAFSLAGLLGLSRYARAEARAVERARHWLDKVGLTSLADEEA
GSLPYGHQRRLEIARAMCTGPLLLCLDEPAAGLNPRESAELNRLLLDIRDENGIGLLLIEHDMSVVMEISDHIVVLDYGK
KIAEGAPAAIKADPAVIRAYLGEPDEEEAR
>Mature_269_residues
SSEALLTVEHVTMRFGGLFAVNDLSFSAAPKEITAVIGPNGAGKTTLFNCITGFYKPQVGRMTLDHPSGPMLLERLDDFA
ISAKAGVARTFQNIRLFARMSVLENLIVAQHTTLMRASAFSLAGLLGLSRYARAEARAVERARHWLDKVGLTSLADEEAG
SLPYGHQRRLEIARAMCTGPLLLCLDEPAAGLNPRESAELNRLLLDIRDENGIGLLLIEHDMSVVMEISDHIVVLDYGKK
IAEGAPAAIKADPAVIRAYLGEPDEEEAR

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI31657092, Length=246, Percent_Identity=26.8292682926829, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI47078218, Length=234, Percent_Identity=27.3504273504274, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI45446740, Length=234, Percent_Identity=27.3504273504274, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI9665248, Length=249, Percent_Identity=26.1044176706827, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI105990541, Length=235, Percent_Identity=27.2340425531915, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI21536376, Length=240, Percent_Identity=28.3333333333333, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI116734710, Length=233, Percent_Identity=27.4678111587983, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1789864, Length=261, Percent_Identity=51.7241379310345, Blast_Score=260, Evalue=6e-71,
Organism=Escherichia coli, GI1786872, Length=254, Percent_Identity=28.3464566929134, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1789593, Length=259, Percent_Identity=30.1158301158301, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1789672, Length=263, Percent_Identity=25.4752851711027, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1788225, Length=253, Percent_Identity=28.8537549407115, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790525, Length=247, Percent_Identity=27.5303643724696, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787029, Length=256, Percent_Identity=26.5625, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788761, Length=262, Percent_Identity=29.0076335877863, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1786563, Length=268, Percent_Identity=29.8507462686567, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87081791, Length=250, Percent_Identity=26.4, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87081709, Length=255, Percent_Identity=25.4901960784314, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1788644, Length=268, Percent_Identity=29.4776119402985, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1790190, Length=269, Percent_Identity=24.907063197026, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI87082268, Length=263, Percent_Identity=27.3764258555133, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1786398, Length=234, Percent_Identity=27.7777777777778, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1789991, Length=251, Percent_Identity=26.6932270916335, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87081782, Length=271, Percent_Identity=27.3062730627306, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1786803, Length=258, Percent_Identity=27.906976744186, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788450, Length=267, Percent_Identity=26.5917602996255, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI48995001, Length=237, Percent_Identity=28.6919831223629, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1788472, Length=261, Percent_Identity=25.6704980842912, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1787370, Length=251, Percent_Identity=27.8884462151394, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788210, Length=241, Percent_Identity=26.1410788381743, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1789032, Length=254, Percent_Identity=26.3779527559055, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI48994997, Length=263, Percent_Identity=25.4752851711027, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790739, Length=255, Percent_Identity=26.6666666666667, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1790544, Length=254, Percent_Identity=25.9842519685039, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787712, Length=232, Percent_Identity=25.8620689655172, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787089, Length=236, Percent_Identity=25.8474576271186, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790467, Length=262, Percent_Identity=24.8091603053435, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790162, Length=245, Percent_Identity=28.1632653061224, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI48994943, Length=246, Percent_Identity=26.0162601626016, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1786253, Length=256, Percent_Identity=25.78125, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786345, Length=248, Percent_Identity=27.8225806451613, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786975, Length=206, Percent_Identity=28.6407766990291, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1789586, Length=252, Percent_Identity=28.968253968254, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI87081834, Length=228, Percent_Identity=26.3157894736842, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787500, Length=239, Percent_Identity=27.6150627615063, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787548, Length=253, Percent_Identity=26.4822134387352, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1789873, Length=235, Percent_Identity=27.6595744680851, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787164, Length=234, Percent_Identity=25.2136752136752, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786981, Length=237, Percent_Identity=30.379746835443, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI145693107, Length=272, Percent_Identity=25.7352941176471, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87082267, Length=261, Percent_Identity=24.1379310344828, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1789962, Length=237, Percent_Identity=27.0042194092827, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1788897, Length=231, Percent_Identity=25.1082251082251, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787792, Length=251, Percent_Identity=24.3027888446215, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1786319, Length=236, Percent_Identity=25.4237288135593, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788506, Length=263, Percent_Identity=27.3764258555133, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787112, Length=233, Percent_Identity=27.4678111587983, Blast_Score=61, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI71992290, Length=222, Percent_Identity=29.2792792792793, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17565586, Length=233, Percent_Identity=26.6094420600858, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17510237, Length=232, Percent_Identity=27.5862068965517, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71983523, Length=232, Percent_Identity=26.7241379310345, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI71983516, Length=232, Percent_Identity=26.7241379310345, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI71983510, Length=232, Percent_Identity=26.7241379310345, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI115533608, Length=238, Percent_Identity=25.2100840336134, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24666092, Length=237, Percent_Identity=27.4261603375527, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24648314, Length=257, Percent_Identity=25.2918287937743, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24643674, Length=246, Percent_Identity=27.2357723577236, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI221512771, Length=239, Percent_Identity=29.2887029288703, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28574744, Length=221, Percent_Identity=29.4117647058824, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI161076682, Length=215, Percent_Identity=25.5813953488372, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI161076684, Length=215, Percent_Identity=25.5813953488372, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24581617, Length=215, Percent_Identity=25.5813953488372, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24581615, Length=215, Percent_Identity=25.5813953488372, Blast_Score=67, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 29321; Mature: 29190

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSEALLTVEHVTMRFGGLFAVNDLSFSAAPKEITAVIGPNGAGKTTLFNCITGFYKPQV
CCCCCEEEHHHHHHHHCCEEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCC
GRMTLDHPSGPMLLERLDDFAISAKAGVARTFQNIRLFARMSVLENLIVAQHTTLMRASA
CCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSLAGLLGLSRYARAEARAVERARHWLDKVGLTSLADEEAGSLPYGHQRRLEIARAMCTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHHCC
PLLLCLDEPAAGLNPRESAELNRLLLDIRDENGIGLLLIEHDMSVVMEISDHIVVLDYGK
CEEEEECCCCCCCCCCHHHHHHHHEEEEECCCCCEEEEEECCCEEEEEECCCEEEEECCC
KIAEGAPAAIKADPAVIRAYLGEPDEEEAR
HHHCCCCCEEECCHHHHHHHCCCCCHHCCC
>Mature Secondary Structure 
SSEALLTVEHVTMRFGGLFAVNDLSFSAAPKEITAVIGPNGAGKTTLFNCITGFYKPQV
CCCCEEEHHHHHHHHCCEEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCC
GRMTLDHPSGPMLLERLDDFAISAKAGVARTFQNIRLFARMSVLENLIVAQHTTLMRASA
CCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FSLAGLLGLSRYARAEARAVERARHWLDKVGLTSLADEEAGSLPYGHQRRLEIARAMCTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCHHHHHHHHHHHHCC
PLLLCLDEPAAGLNPRESAELNRLLLDIRDENGIGLLLIEHDMSVVMEISDHIVVLDYGK
CEEEEECCCCCCCCCCHHHHHHHHEEEEECCCCCEEEEEECCCEEEEEECCCEEEEECCC
KIAEGAPAAIKADPAVIRAYLGEPDEEEAR
HHHCCCCCEEECCHHHHHHHCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]