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The map label for this gene is spoT [H]

Identifier: 83310796

GI number: 83310796

Start: 1844505

End: 1845053

Strand: Reverse

Name: spoT [H]

Synonym: amb1697

Alternate gene names: 83310796

Gene position: 1845053-1844505 (Counterclockwise)

Preceding gene: 83310797

Following gene: 83310795

Centisome position: 37.15

GC content: 66.67

Gene sequence:

>549_bases
ATGACGGCAAACCACCCCTGGTCCCAGGACGCCTTCTTGGCCACCCTGGCCTTCGCCGCCGAACGGCATGGCGACCAGAA
GACGCCCGCCGGCTTCCCCTATGTCACCCACCTGGCCTCGGTGGCCCAGGAGGTGATGGCCGCCCTGGCCGCGGAGCCCG
GACGCAATGGCACCCTGGCGGTGACGGCCGCCCTACTCCATGACGTGATCGAGGATACCGCCACCAGCCCCGAGATGGTG
GCGGAGCGGTTCGGCCCCGATATTCTGGCGGCTGTCATGGCCTTGACCAAGAATCCCGATCTTCCAAAGGAACGGCGCAT
GCCCGACAGCTTGGAGCGCATTCGGGCTCAGCCGCAGGAAGTCTGGATGGTCAAACTGGCCGATCGCATCGTCAATCTCG
GCCCGCCGCCCGCCCACTGGTCTCGGGACAAGATGGCCGCCTACCGGGCCGAGGCGGAGGCCATCCTGTCCGCCCTGCGT
GATGCGAGCCCGTTCCTCGCTTGCCGGCTGGAACTGCGCATCACCGGATACGGCATCCATCTGAAATGA

Upstream 100 bases:

>100_bases
AATAGCGGCCGCCGCATCAATTTTTGCATGCTACGTACTATACATGATGTCCGTCGGCAGAGCGTAGATGTAAAATGCGA
TATATACTTGATGGTGAAGC

Downstream 100 bases:

>100_bases
ACCCCATCGTGTCGGCTCTTGGCCGCTACCACCGTATCTATGGCGATTTTGGCGCAGCACGGGCGGCCGCCATCATTTAT
ATGCCGCTGGCGGCGTTTAA

Product: guanosine polyphosphate pyrophosphohydrolase/synthetase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 182; Mature: 181

Protein sequence:

>182_residues
MTANHPWSQDAFLATLAFAAERHGDQKTPAGFPYVTHLASVAQEVMAALAAEPGRNGTLAVTAALLHDVIEDTATSPEMV
AERFGPDILAAVMALTKNPDLPKERRMPDSLERIRAQPQEVWMVKLADRIVNLGPPPAHWSRDKMAAYRAEAEAILSALR
DASPFLACRLELRITGYGIHLK

Sequences:

>Translated_182_residues
MTANHPWSQDAFLATLAFAAERHGDQKTPAGFPYVTHLASVAQEVMAALAAEPGRNGTLAVTAALLHDVIEDTATSPEMV
AERFGPDILAAVMALTKNPDLPKERRMPDSLERIRAQPQEVWMVKLADRIVNLGPPPAHWSRDKMAAYRAEAEAILSALR
DASPFLACRLELRITGYGIHLK
>Mature_181_residues
TANHPWSQDAFLATLAFAAERHGDQKTPAGFPYVTHLASVAQEVMAALAAEPGRNGTLAVTAALLHDVIEDTATSPEMVA
ERFGPDILAAVMALTKNPDLPKERRMPDSLERIRAQPQEVWMVKLADRIVNLGPPPAHWSRDKMAAYRAEAEAILSALRD
ASPFLACRLELRITGYGIHLK

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=125, Percent_Identity=36.8, Blast_Score=64, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI25143118, Length=164, Percent_Identity=28.0487804878049, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24650996, Length=166, Percent_Identity=31.3253012048193, Blast_Score=80, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 19888; Mature: 19757

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTANHPWSQDAFLATLAFAAERHGDQKTPAGFPYVTHLASVAQEVMAALAAEPGRNGTLA
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHH
VTAALLHDVIEDTATSPEMVAERFGPDILAAVMALTKNPDLPKERRMPDSLERIRAQPQE
HHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHCCHHH
VWMVKLADRIVNLGPPPAHWSRDKMAAYRAEAEAILSALRDASPFLACRLELRITGYGIH
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEEE
LK
EC
>Mature Secondary Structure 
TANHPWSQDAFLATLAFAAERHGDQKTPAGFPYVTHLASVAQEVMAALAAEPGRNGTLA
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHH
VTAALLHDVIEDTATSPEMVAERFGPDILAAVMALTKNPDLPKERRMPDSLERIRAQPQE
HHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHCCHHH
VWMVKLADRIVNLGPPPAHWSRDKMAAYRAEAEAILSALRDASPFLACRLELRITGYGIH
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEEE
LK
EC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]