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The map label for this gene is gabT [C]

Identifier: 83310783

GI number: 83310783

Start: 1824489

End: 1825868

Strand: Reverse

Name: gabT [C]

Synonym: amb1684

Alternate gene names: 83310783

Gene position: 1825868-1824489 (Counterclockwise)

Preceding gene: 83310784

Following gene: 83310782

Centisome position: 36.76

GC content: 67.83

Gene sequence:

>1380_bases
ATGCAGACCCACGCCAGCAACCGAACCATCGCCGAAAAGGACGTGGACTCGGTCCTGCACCCCTATACCAATCTGAAGCG
CCATCCCGAGGTGGGGCCGCTGGTCATCACCCGAGGCCAGGGCGTGCGGGTCTATGACGAGGCGGGGCGCGACTATATCG
AGGGCCTGGCCGGGCTGTGGTGCACGTCGTTGGGCTGGGGCGAGGAGCGGCTGGTGGAAGCCGCCGCCCGCCAGATGCGA
CAACTCCCGTTTTATCATCTGTTCAGCCACAAGACCCACGACGTGGGCGTCGAACTGTGCGCCCGCCTGCTGGCCATGGC
TCCTGTCCCCATGTCCAAGGTGTTCCTGGCCGGATCGGGGTCGGAAGCCAACGATACCGCCATCAAGCTGATCCACTACC
GCGCCAATGCCTTGGGGACGCCCGACAAGAAGAAGATCATCGCCCGGGAGAAGGCCTATCACGGCGTCACCGTGGCCACC
GCCTCGCTGACCGGGCTGGTCAACAACCAGCGCTCGTTCGACCTGCCCATTCCCGGCGTGCTGCGGGCCGCCTGCCCTCA
CCACTACCGCTTCGCCAAGGATGGCGAGAGCGAGGAGGACTTTTCCACCCGTCTCGCCGGGGAGCTGGAGGCGATGATCC
TGGCCGAGGGGCCCGACACCGTCGCCGCCTTCTTCGCCGAGCCGGTGATGGGCGCCGGCGGCGTCATCGTTCCCCCCGCC
GGCTACTTCCCCAAGATCCAGGCGGTGCTCGACAGATATGATGTGCTGCTGGTGGTCGACGAGGTGATTTGCGGCTTCGG
CCGCACCGGCAAGATGTTCGGCACCGAGACCTTCGGCATCCGCCCCGACATGATGACGCTCGCCAAGGGGCTGTCCTCGG
GCTATGCCCCGATCTCGGCCCTGATGGTCAATGAGCGGGTTTACGGCCCCGTCGCCGAGGAATCGGGACGCATCGGCGTG
TTCGGCCACGGCTACACCTATGGCGGCCACCCGGTGTCGGCCGCGGTGGCGCTGGAGACGCTGAACATCTATGCCGAGCG
CGACATCCTGGCCCAGGTGGCCGAGGTCGGCCCGGTGCTGCAGGACGGCTTGCGGGCGTTGCGCGGCCACCCCTTGGTGG
GCGAAGCCCGCGGCGTGGGGCTGATCGGCGCCGTGGAACTGGTGGCGGACAAGGCCAACCGGGCCCCCTTCCCGCCCGAA
CTGGCCGTGGGCGCCCGGGTGGTGGCCAAGGCCCAGGCCAAGGGCGTGATCCTGCGCGCCATGGGCGACGCGGTGGCCTT
CGCTCCGCCTCTGGTCATCTCGAAGGCCGATATCGCCGAGATGCTGCGGCGTTTCGGCCTGGCGCTTGACGAAGCTCATG
GCGAGCTTGGACGCGGATAA

Upstream 100 bases:

>100_bases
GTCTACGCCTACAAGATGAAGTCGCACACCAAGCCCTGAGGCTTGATCCCCCGGGGGGAGCGGTTCATGCTTCGCCTTCC
GCTTGACCCCGGGAGGCCGC

Downstream 100 bases:

>100_bases
CTATGTGATACTTCAGTCGAAAGGCCCGGCGCATTACAGCTTGTACACTTGCGCCCCGGCTTTTCGATCCTCTAGGATCG
GCGCACCGGCCCGAGACCCC

Product: aminotransferase

Products: L-Glutamate; Succinic semialdehyde [C]

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MQTHASNRTIAEKDVDSVLHPYTNLKRHPEVGPLVITRGQGVRVYDEAGRDYIEGLAGLWCTSLGWGEERLVEAAARQMR
QLPFYHLFSHKTHDVGVELCARLLAMAPVPMSKVFLAGSGSEANDTAIKLIHYRANALGTPDKKKIIAREKAYHGVTVAT
ASLTGLVNNQRSFDLPIPGVLRAACPHHYRFAKDGESEEDFSTRLAGELEAMILAEGPDTVAAFFAEPVMGAGGVIVPPA
GYFPKIQAVLDRYDVLLVVDEVICGFGRTGKMFGTETFGIRPDMMTLAKGLSSGYAPISALMVNERVYGPVAEESGRIGV
FGHGYTYGGHPVSAAVALETLNIYAERDILAQVAEVGPVLQDGLRALRGHPLVGEARGVGLIGAVELVADKANRAPFPPE
LAVGARVVAKAQAKGVILRAMGDAVAFAPPLVISKADIAEMLRRFGLALDEAHGELGRG

Sequences:

>Translated_459_residues
MQTHASNRTIAEKDVDSVLHPYTNLKRHPEVGPLVITRGQGVRVYDEAGRDYIEGLAGLWCTSLGWGEERLVEAAARQMR
QLPFYHLFSHKTHDVGVELCARLLAMAPVPMSKVFLAGSGSEANDTAIKLIHYRANALGTPDKKKIIAREKAYHGVTVAT
ASLTGLVNNQRSFDLPIPGVLRAACPHHYRFAKDGESEEDFSTRLAGELEAMILAEGPDTVAAFFAEPVMGAGGVIVPPA
GYFPKIQAVLDRYDVLLVVDEVICGFGRTGKMFGTETFGIRPDMMTLAKGLSSGYAPISALMVNERVYGPVAEESGRIGV
FGHGYTYGGHPVSAAVALETLNIYAERDILAQVAEVGPVLQDGLRALRGHPLVGEARGVGLIGAVELVADKANRAPFPPE
LAVGARVVAKAQAKGVILRAMGDAVAFAPPLVISKADIAEMLRRFGLALDEAHGELGRG
>Mature_459_residues
MQTHASNRTIAEKDVDSVLHPYTNLKRHPEVGPLVITRGQGVRVYDEAGRDYIEGLAGLWCTSLGWGEERLVEAAARQMR
QLPFYHLFSHKTHDVGVELCARLLAMAPVPMSKVFLAGSGSEANDTAIKLIHYRANALGTPDKKKIIAREKAYHGVTVAT
ASLTGLVNNQRSFDLPIPGVLRAACPHHYRFAKDGESEEDFSTRLAGELEAMILAEGPDTVAAFFAEPVMGAGGVIVPPA
GYFPKIQAVLDRYDVLLVVDEVICGFGRTGKMFGTETFGIRPDMMTLAKGLSSGYAPISALMVNERVYGPVAEESGRIGV
FGHGYTYGGHPVSAAVALETLNIYAERDILAQVAEVGPVLQDGLRALRGHPLVGEARGVGLIGAVELVADKANRAPFPPE
LAVGARVVAKAQAKGVILRAMGDAVAFAPPLVISKADIAEMLRRFGLALDEAHGELGRG

Specific function: 4-aminobutyrate (GABA) degradation. [C]

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI37574042, Length=435, Percent_Identity=30.5747126436782, Blast_Score=166, Evalue=6e-41,
Organism=Homo sapiens, GI226442705, Length=435, Percent_Identity=30.5747126436782, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI24119277, Length=419, Percent_Identity=31.0262529832936, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI226442709, Length=397, Percent_Identity=31.2342569269521, Blast_Score=153, Evalue=4e-37,
Organism=Homo sapiens, GI13994255, Length=444, Percent_Identity=25.6756756756757, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI4557809, Length=415, Percent_Identity=26.0240963855422, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI284507298, Length=224, Percent_Identity=30.3571428571429, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI188536080, Length=363, Percent_Identity=26.4462809917355, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI38679950, Length=363, Percent_Identity=26.4462809917355, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI38679946, Length=363, Percent_Identity=26.4462809917355, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1789016, Length=431, Percent_Identity=31.0904872389791, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI145693181, Length=357, Percent_Identity=32.7731092436975, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1786991, Length=441, Percent_Identity=29.9319727891156, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1788044, Length=420, Percent_Identity=29.7619047619048, Blast_Score=162, Evalue=6e-41,
Organism=Escherichia coli, GI1789759, Length=417, Percent_Identity=29.7362110311751, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI1787560, Length=415, Percent_Identity=29.1566265060241, Blast_Score=126, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI71992977, Length=445, Percent_Identity=26.7415730337079, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI25144271, Length=412, Percent_Identity=27.1844660194175, Blast_Score=141, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI32564660, Length=432, Percent_Identity=27.0833333333333, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI25144274, Length=251, Percent_Identity=32.2709163346614, Blast_Score=125, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6324432, Length=426, Percent_Identity=28.8732394366197, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6323470, Length=457, Percent_Identity=26.6958424507659, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6324386, Length=480, Percent_Identity=25.625, Blast_Score=138, Evalue=2e-33,
Organism=Drosophila melanogaster, GI28574759, Length=447, Percent_Identity=28.1879194630872, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI161085790, Length=433, Percent_Identity=28.175519630485, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI21356575, Length=423, Percent_Identity=29.3144208037825, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI21357415, Length=454, Percent_Identity=26.8722466960352, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI281366494, Length=443, Percent_Identity=23.9277652370203, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24667139, Length=443, Percent_Identity=23.9277652370203, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24667143, Length=443, Percent_Identity=23.9277652370203, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.19 [C]

Molecular weight: Translated: 49063; Mature: 49063

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTHASNRTIAEKDVDSVLHPYTNLKRHPEVGPLVITRGQGVRVYDEAGRDYIEGLAGLW
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEHHHCCHHHHHHHHHHH
CTSLGWGEERLVEAAARQMRQLPFYHLFSHKTHDVGVELCARLLAMAPVPMSKVFLAGSG
HHCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHEEEECCC
SEANDTAIKLIHYRANALGTPDKKKIIAREKAYHGVTVATASLTGLVNNQRSFDLPIPGV
CCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCEEEEHHHHHHHHCCCCCCCCCCCHH
LRAACPHHYRFAKDGESEEDFSTRLAGELEAMILAEGPDTVAAFFAEPVMGAGGVIVPPA
HHHHCCCCCEECCCCCCCHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHCCCCCEEECCC
GYFPKIQAVLDRYDVLLVVDEVICGFGRTGKMFGTETFGIRPDMMTLAKGLSSGYAPISA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHH
LMVNERVYGPVAEESGRIGVFGHGYTYGGHPVSAAVALETLNIYAERDILAQVAEVGPVL
HEECCHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
QDGLRALRGHPLVGEARGVGLIGAVELVADKANRAPFPPELAVGARVVAKAQAKGVILRA
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCEEEEE
MGDAVAFAPPLVISKADIAEMLRRFGLALDEAHGELGRG
CCCHHHHCCCCEECHHHHHHHHHHHCCHHHHHCCCCCCC
>Mature Secondary Structure
MQTHASNRTIAEKDVDSVLHPYTNLKRHPEVGPLVITRGQGVRVYDEAGRDYIEGLAGLW
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEHHHCCHHHHHHHHHHH
CTSLGWGEERLVEAAARQMRQLPFYHLFSHKTHDVGVELCARLLAMAPVPMSKVFLAGSG
HHCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHEEEECCC
SEANDTAIKLIHYRANALGTPDKKKIIAREKAYHGVTVATASLTGLVNNQRSFDLPIPGV
CCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCEEEEHHHHHHHHCCCCCCCCCCCHH
LRAACPHHYRFAKDGESEEDFSTRLAGELEAMILAEGPDTVAAFFAEPVMGAGGVIVPPA
HHHHCCCCCEECCCCCCCHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHCCCCCEEECCC
GYFPKIQAVLDRYDVLLVVDEVICGFGRTGKMFGTETFGIRPDMMTLAKGLSSGYAPISA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHH
LMVNERVYGPVAEESGRIGVFGHGYTYGGHPVSAAVALETLNIYAERDILAQVAEVGPVL
HEECCHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
QDGLRALRGHPLVGEARGVGLIGAVELVADKANRAPFPPELAVGARVVAKAQAKGVILRA
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCEEEEE
MGDAVAFAPPLVISKADIAEMLRRFGLALDEAHGELGRG
CCCHHHHCCCCEECHHHHHHHHHHHCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal 5'-phosphate lysine-residue [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 3.3 {4-aminobutanoate}} 1.1 {4-aminobutanoate}} 1.3 {2-oxoglutarate}} 0.7 {2-oxoglutarate}} [C]

Substrates: 4-Aminobutanoate; 2-Oxoglutarate [C]

Specific reaction: 4-Aminobutanoate + 2-Oxoglutarate --> L-Glutamate + Succinic semialdehyde [C]

General reaction: Amino group transfer [C]

Inhibitor: 2-Oxoglutarate; Lysyl reagents; SH-group reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8796346; 9163424 [H]