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The map label for this gene is kgtP [H]

Identifier: 83310753

GI number: 83310753

Start: 1791211

End: 1792629

Strand: Reverse

Name: kgtP [H]

Synonym: amb1654

Alternate gene names: 83310753

Gene position: 1792629-1791211 (Counterclockwise)

Preceding gene: 83310755

Following gene: 83310752

Centisome position: 36.09

GC content: 63.71

Gene sequence:

>1419_bases
TTGGCCCGCCCCTTGCGTTTGCCCTCCGCAAAATCGCCCGCGCCCTGCGGGCCAATGTGGGAGGACCGAACGTTGAGTCA
CATGGATACGACCATAGCCCCGGCCCCAGTCACTCCGGTGACCGGCAGCCACGACATGGAGAGCAAAGACACCAAGGGCA
AGATCCTGATGGTGTTCAAGGGCTCGGTGGGCAATCTGGTGGAATACTACGACTGGTACGTCTACTCCGCCTTCTCGCTG
TATTTCTCCAAGTACTTCTTTCCCGGCGACGACCCCACCGTGCAGCTGCTCAACACCTCGGCCATCTTCGCCCTGGGCTT
CTTCATGCGTCCGCTGGGCGGCTGGCTGCTGGGCACCCATGCCGACCGCAAGGGGCGCAAGGCGGCCCTGCTGGTCTCGG
TCAGCATGATGTGCGCCGGATCGCTGATCATCGCCGTGATGCCCGGCTACAACAGCATCGGCGTGGCGGCGCCCATCGCG
CTGATCCTTGCCCGCCTGCTGCAAGGCCTCAGCCTGGGCGGCGAATACGGCTCGGCCGCCACCTATCTGTCGGAAATCGC
CACCAAGGACCGGCGCGGCTTTTATTCCAGCTTCCAGTACGTGACCCTGATCATGGGACAATTGCTGGCGCTGGGCGTGC
TGATGGCGCTGCAGCGGGTGTTTCTCACCACCGCCGAGCTGGAAGCCTGGGGCTGGCGCATTCCCTTCGTCATCGGCGGG
CTGTGCGCCATCGTCGCCATCTACCTGCGGAGCTCCATGGAAGAGACCGAGTCCTTCGAGCACCACAAGGGCGACCGGGT
GGGTGAAAGCCGCATCCGCGCCCTGATGCGCCACCCGCGCGAGGTGCTGACGGTGATCGGCCTGACCATGGGCGGCACGG
TGGCCTTCTACACCTTCACCACCTACATGCAGAAATATCTGGTCAACACCGCCGGTTTCTCCAAAAGCGACGCCACCATG
ATTTCGGCGGCGGCCACCTTCGTCTACATGCTGATGCATCCCCTGGTCGGCCACATTTCCGACAAGGTGGGCCGGCGCGC
CGTGCTAATCGCCTTCGGCGTGATGAGCACGCTGTGCACCGTGCCGATCCTCACCGCCCTGGGCCAGACCCATGATTCCG
TCACCGCCTTCTTCCTGGTGCTGTCGGGACTGACCATCGTCTCGGGCTATTCCGCCATCAACGCCGTGGTCAAGGCCGAG
CTGTTCCCGGTGCAGATCCGCGCCCTGGGCGTCGGCCTGCCCTTCGCCATCGGTGTGTCGCTGTTCGGCGGCACCGCCGA
GTACATCGCCCTGTGGTTCAAGAGCATGGGCAACGAGACCTGGTTCTACTGGTACGTGACCGGCTGCTGCCTGTGCGGAT
TGCTGCTGTTCGTCGGCATGAAGGACACCAAGAAGACCTCGCTGATCGACCAGGACTGA

Upstream 100 bases:

>100_bases
CGCCGATACCCCAGGAGCGCATCGGCGAAGAACCGCCGAACACCCCGCACCCCGATAAATTTTATCGTTGATTTTCAACG
AGATACACCAAGGGCGCCAT

Downstream 100 bases:

>100_bases
CCCTCCTGTCCCCCGGCGGCTCCAGCCCGGAGCCGCCGGTTTTTTCATTTTCCAAGGACAGCATCATGACCGGACCCTTG
TCGCATCTTCGCGTTCTCGA

Product: major facilitator superfamily permease

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MARPLRLPSAKSPAPCGPMWEDRTLSHMDTTIAPAPVTPVTGSHDMESKDTKGKILMVFKGSVGNLVEYYDWYVYSAFSL
YFSKYFFPGDDPTVQLLNTSAIFALGFFMRPLGGWLLGTHADRKGRKAALLVSVSMMCAGSLIIAVMPGYNSIGVAAPIA
LILARLLQGLSLGGEYGSAATYLSEIATKDRRGFYSSFQYVTLIMGQLLALGVLMALQRVFLTTAELEAWGWRIPFVIGG
LCAIVAIYLRSSMEETESFEHHKGDRVGESRIRALMRHPREVLTVIGLTMGGTVAFYTFTTYMQKYLVNTAGFSKSDATM
ISAAATFVYMLMHPLVGHISDKVGRRAVLIAFGVMSTLCTVPILTALGQTHDSVTAFFLVLSGLTIVSGYSAINAVVKAE
LFPVQIRALGVGLPFAIGVSLFGGTAEYIALWFKSMGNETWFYWYVTGCCLCGLLLFVGMKDTKKTSLIDQD

Sequences:

>Translated_472_residues
MARPLRLPSAKSPAPCGPMWEDRTLSHMDTTIAPAPVTPVTGSHDMESKDTKGKILMVFKGSVGNLVEYYDWYVYSAFSL
YFSKYFFPGDDPTVQLLNTSAIFALGFFMRPLGGWLLGTHADRKGRKAALLVSVSMMCAGSLIIAVMPGYNSIGVAAPIA
LILARLLQGLSLGGEYGSAATYLSEIATKDRRGFYSSFQYVTLIMGQLLALGVLMALQRVFLTTAELEAWGWRIPFVIGG
LCAIVAIYLRSSMEETESFEHHKGDRVGESRIRALMRHPREVLTVIGLTMGGTVAFYTFTTYMQKYLVNTAGFSKSDATM
ISAAATFVYMLMHPLVGHISDKVGRRAVLIAFGVMSTLCTVPILTALGQTHDSVTAFFLVLSGLTIVSGYSAINAVVKAE
LFPVQIRALGVGLPFAIGVSLFGGTAEYIALWFKSMGNETWFYWYVTGCCLCGLLLFVGMKDTKKTSLIDQD
>Mature_471_residues
ARPLRLPSAKSPAPCGPMWEDRTLSHMDTTIAPAPVTPVTGSHDMESKDTKGKILMVFKGSVGNLVEYYDWYVYSAFSLY
FSKYFFPGDDPTVQLLNTSAIFALGFFMRPLGGWLLGTHADRKGRKAALLVSVSMMCAGSLIIAVMPGYNSIGVAAPIAL
ILARLLQGLSLGGEYGSAATYLSEIATKDRRGFYSSFQYVTLIMGQLLALGVLMALQRVFLTTAELEAWGWRIPFVIGGL
CAIVAIYLRSSMEETESFEHHKGDRVGESRIRALMRHPREVLTVIGLTMGGTVAFYTFTTYMQKYLVNTAGFSKSDATMI
SAAATFVYMLMHPLVGHISDKVGRRAVLIAFGVMSTLCTVPILTALGQTHDSVTAFFLVLSGLTIVSGYSAINAVVKAEL
FPVQIRALGVGLPFAIGVSLFGGTAEYIALWFKSMGNETWFYWYVTGCCLCGLLLFVGMKDTKKTSLIDQD

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=410, Percent_Identity=52.6829268292683, Blast_Score=409, Evalue=1e-115,
Organism=Escherichia coli, GI1790550, Length=422, Percent_Identity=30.8056872037915, Blast_Score=209, Evalue=4e-55,
Organism=Escherichia coli, GI1788292, Length=409, Percent_Identity=30.3178484107579, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1789941, Length=447, Percent_Identity=29.7539149888143, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI87082231, Length=257, Percent_Identity=27.6264591439689, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI87082404, Length=342, Percent_Identity=22.8070175438596, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 51446; Mature: 51315

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARPLRLPSAKSPAPCGPMWEDRTLSHMDTTIAPAPVTPVTGSHDMESKDTKGKILMVFK
CCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEE
GSVGNLVEYYDWYVYSAFSLYFSKYFFPGDDPTVQLLNTSAIFALGFFMRPLGGWLLGTH
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHCCCEEECC
ADRKGRKAALLVSVSMMCAGSLIIAVMPGYNSIGVAAPIALILARLLQGLSLGGEYGSAA
CCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHH
TYLSEIATKDRRGFYSSFQYVTLIMGQLLALGVLMALQRVFLTTAELEAWGWRIPFVIGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LCAIVAIYLRSSMEETESFEHHKGDRVGESRIRALMRHPREVLTVIGLTMGGTVAFYTFT
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHH
TYMQKYLVNTAGFSKSDATMISAAATFVYMLMHPLVGHISDKVGRRAVLIAFGVMSTLCT
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
VPILTALGQTHDSVTAFFLVLSGLTIVSGYSAINAVVKAELFPVQIRALGVGLPFAIGVS
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCHHHHHHH
LFGGTAEYIALWFKSMGNETWFYWYVTGCCLCGLLLFVGMKDTKKTSLIDQD
HCCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
ARPLRLPSAKSPAPCGPMWEDRTLSHMDTTIAPAPVTPVTGSHDMESKDTKGKILMVFK
CCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEE
GSVGNLVEYYDWYVYSAFSLYFSKYFFPGDDPTVQLLNTSAIFALGFFMRPLGGWLLGTH
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHCCCEEECC
ADRKGRKAALLVSVSMMCAGSLIIAVMPGYNSIGVAAPIALILARLLQGLSLGGEYGSAA
CCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHH
TYLSEIATKDRRGFYSSFQYVTLIMGQLLALGVLMALQRVFLTTAELEAWGWRIPFVIGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LCAIVAIYLRSSMEETESFEHHKGDRVGESRIRALMRHPREVLTVIGLTMGGTVAFYTFT
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHH
TYMQKYLVNTAGFSKSDATMISAAATFVYMLMHPLVGHISDKVGRRAVLIAFGVMSTLCT
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
VPILTALGQTHDSVTAFFLVLSGLTIVSGYSAINAVVKAELFPVQIRALGVGLPFAIGVS
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCHHHHHHH
LFGGTAEYIALWFKSMGNETWFYWYVTGCCLCGLLLFVGMKDTKKTSLIDQD
HCCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]