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The map label for this gene is rfbD [C]

Identifier: 83310572

GI number: 83310572

Start: 1610938

End: 1611828

Strand: Reverse

Name: rfbD [C]

Synonym: amb1473

Alternate gene names: 83310572

Gene position: 1611828-1610938 (Counterclockwise)

Preceding gene: 83310574

Following gene: 83310566

Centisome position: 32.45

GC content: 70.71

Gene sequence:

>891_bases
ATGCACATTCTCGTAACCGGGGCTGACGGAAGAGTGGGACGCGCGCTGGCCCGGGCCGCACTCCCGCAGGGCGCGACCCT
CACGGCGCTGGGGCGACAGCAATTGGACATCACCGACCCGCAAGCGGTGGACCGGCTGTTGACCCTCCACGCCTGTTCCG
TGGTGGTCAACACCGCTGCCTTCACCGCCGTCGATCGCGCCGAGACCGAGACGGCAGCCGCCATGGCCATCAATGGCGAA
GGGCCGGCTCATTTGGCCCGCGCCTGCGCCGCCCGGGGCATCCCGCTGATTCATCTGTCCACCGATTACGTCTTCGATGG
CCGCTCGCCCGAGCCCTACCGCGAGGACGCCCCCATGGCGCCGCTTTCCGTCTATGGCGCCAGCAAGGCGGCGGGGGAAG
AGGCCGTGCGCTGGCTGCAGCCCGACCACGCCATCTTGCGGGTATCGTGGCTGTACGGCGGCGAACGGGGCGACTTCGTC
CGCGCCATGGTGGGCGCCATCCGGGCCGGACGCCCCTTGCGGGTGGTCGACGACCAGATCGGCGGCCTCACCCATTCCGA
CAGCGTGGCTACGGCCGTCCTGGCCCTGGCCGACAAGATGCTGCGCCAGTCCCTGGAGCGGGGAACCTACCACTTCGCCG
CCGGCGGCGCCGCCTCTTGGCACGAGATCGCCGCCCTGATCCTGTCCGAGCTGACCCGGGCGGGCATCGGCCCGGTTCCG
CTGGAGGCCACTTCCGCCGCGGCTTACGGGCTGCCGGCGGCCCGCCCGGCCAATTCCCTGCTCGATTGCGGCAAGTTCGA
CCGACTGGTCGGGGCCGAGCGGCCCCATTGGCAAGACAGGCTGATCCCCGAATTGCGGCGCATCCTGGACGAGCCGGTCA
AGCCGGCGTAG

Upstream 100 bases:

>100_bases
ATGTTTTCTCCTCCTTTCTCCCGGTCTATATAGACGCAATATGGACATGGGCACCAGTCGGAGAAATTCCCGGCAGGTCT
GACGGGGGATATGGCGGCTG

Downstream 100 bases:

>100_bases
TCCAGTACTGCCCCCATTCCGCCCGCTCCTGGGCCAGTTGGGGAAAGCCCCCCTTGGCCAGCAGGGCTGCGCGCACCCCA
CGCCAGAAGGGATAAGCCTG

Product: dTDP-6-deoxy-L-mannose-dehydrogenase

Products: NA

Alternate protein names: dTDP-4-keto-L-rhamnose reductase; dTDP-6-deoxy-L-lyxo-4-hexulose reductase; dTDP-6-deoxy-L-mannose dehydrogenase; dTDP-L-rhamnose synthase [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MHILVTGADGRVGRALARAALPQGATLTALGRQQLDITDPQAVDRLLTLHACSVVVNTAAFTAVDRAETETAAAMAINGE
GPAHLARACAARGIPLIHLSTDYVFDGRSPEPYREDAPMAPLSVYGASKAAGEEAVRWLQPDHAILRVSWLYGGERGDFV
RAMVGAIRAGRPLRVVDDQIGGLTHSDSVATAVLALADKMLRQSLERGTYHFAAGGAASWHEIAALILSELTRAGIGPVP
LEATSAAAYGLPAARPANSLLDCGKFDRLVGAERPHWQDRLIPELRRILDEPVKPA

Sequences:

>Translated_296_residues
MHILVTGADGRVGRALARAALPQGATLTALGRQQLDITDPQAVDRLLTLHACSVVVNTAAFTAVDRAETETAAAMAINGE
GPAHLARACAARGIPLIHLSTDYVFDGRSPEPYREDAPMAPLSVYGASKAAGEEAVRWLQPDHAILRVSWLYGGERGDFV
RAMVGAIRAGRPLRVVDDQIGGLTHSDSVATAVLALADKMLRQSLERGTYHFAAGGAASWHEIAALILSELTRAGIGPVP
LEATSAAAYGLPAARPANSLLDCGKFDRLVGAERPHWQDRLIPELRRILDEPVKPA
>Mature_296_residues
MHILVTGADGRVGRALARAALPQGATLTALGRQQLDITDPQAVDRLLTLHACSVVVNTAAFTAVDRAETETAAAMAINGE
GPAHLARACAARGIPLIHLSTDYVFDGRSPEPYREDAPMAPLSVYGASKAAGEEAVRWLQPDHAILRVSWLYGGERGDFV
RAMVGAIRAGRPLRVVDDQIGGLTHSDSVATAVLALADKMLRQSLERGTYHFAAGGAASWHEIAALILSELTRAGIGPVP
LEATSAAAYGLPAARPANSLLDCGKFDRLVGAERPHWQDRLIPELRRILDEPVKPA

Specific function: Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4- hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well [H]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=289, Percent_Identity=32.1799307958478, Blast_Score=111, Evalue=9e-25,
Organism=Homo sapiens, GI11034825, Length=287, Percent_Identity=31.7073170731707, Blast_Score=110, Evalue=1e-24,
Organism=Escherichia coli, GI1788352, Length=295, Percent_Identity=35.9322033898305, Blast_Score=163, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: =1.1.1.133 [H]

Molecular weight: Translated: 31391; Mature: 31391

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHILVTGADGRVGRALARAALPQGATLTALGRQQLDITDPQAVDRLLTLHACSVVVNTAA
CEEEEECCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
FTAVDRAETETAAAMAINGEGPAHLARACAARGIPLIHLSTDYVFDGRSPEPYREDAPMA
HHHHHHHHHHHHEEEEECCCCHHHHHHHHHHCCCEEEEEECCEEECCCCCCCCCCCCCCC
PLSVYGASKAAGEEAVRWLQPDHAILRVSWLYGGERGDFVRAMVGAIRAGRPLRVVDDQI
CHHHCCCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCEEEHHHHH
GGLTHSDSVATAVLALADKMLRQSLERGTYHFAAGGAASWHEIAALILSELTRAGIGPVP
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCC
LEATSAAAYGLPAARPANSLLDCGKFDRLVGAERPHWQDRLIPELRRILDEPVKPA
CCCCCHHHCCCCCCCCCHHHHHCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MHILVTGADGRVGRALARAALPQGATLTALGRQQLDITDPQAVDRLLTLHACSVVVNTAA
CEEEEECCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
FTAVDRAETETAAAMAINGEGPAHLARACAARGIPLIHLSTDYVFDGRSPEPYREDAPMA
HHHHHHHHHHHHEEEEECCCCHHHHHHHHHHCCCEEEEEECCEEECCCCCCCCCCCCCCC
PLSVYGASKAAGEEAVRWLQPDHAILRVSWLYGGERGDFVRAMVGAIRAGRPLRVVDDQI
CHHHCCCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCEEEHHHHH
GGLTHSDSVATAVLALADKMLRQSLERGTYHFAAGGAASWHEIAALILSELTRAGIGPVP
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCCC
LEATSAAAYGLPAARPANSLLDCGKFDRLVGAERPHWQDRLIPELRRILDEPVKPA
CCCCCHHHCCCCCCCCCHHHHHCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]