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The map label for this gene is pleD [H]

Identifier: 83310566

GI number: 83310566

Start: 1604327

End: 1606231

Strand: Reverse

Name: pleD [H]

Synonym: amb1467

Alternate gene names: 83310566

Gene position: 1606231-1604327 (Counterclockwise)

Preceding gene: 83310572

Following gene: 83310559

Centisome position: 32.34

GC content: 63.46

Gene sequence:

>1905_bases
ATGCCGATTCCGCCTGCAGCATCTTGGGGGAAGTCACGGATTATGAGCTTGAACTCAAAGGTCACGATGTTTTTCGTGGC
CATGGCCGTCAGCTTGGTCTTCATGCTGGTGGCGATCAATCTGTACGCCTTTCGCTCGTTTTCGATCGCGTCGGCCACTG
ACCACATCCGCACCGCCGCCGAAATCGTTCGCGTCCACCTGACCGAATCCATGATCAACGGCGTCATCGACAAGCGGGAA
AGCTTTCTCAGCCGCCTGATGGACGTCCAGGGCCTGACCTCGGCCCAGGTGGTCCGCTCGCCCCTGGTGGAGCAGCAGTT
TGGCAAGGGCCTGGGGCGCGAGACTCCCAACGATGAGGTGGAGCAGCAGGTTCTCCACGACGGCAAGCCCGTCTACCGCG
TCGAGGAAAGCCCCAACGGCACCATCTTCCGCGGCACCATTCCCTTCGTCGCCTCGACCCGCGGCTCGCCCAACTGCCTG
CAATGCCATCAGGTGCAGGAAGGCGCGGTGCTGGGCGCGGTGACGCTGTCCATGTCCATCGAGGACCTGAAGTCCAAGGC
CCTGCTGACCGTCGCCGGAATCGTCGGAATCGTGGTGCTGTTCAGCGCCGCGACCTTCTTCCTGATCCGGCGCGTCATCC
AGCCGGTCTCGACCACCGCCCTCAATGTGGAACTGGCGGTCCAGCGGGCGCTTCGCGGCGACTTCAAGGGCCAGGTCAGC
CAGCGGACCGAGGACGAGGTCGGCCAGATCGCCGCCGACATGAACCGCCTTCTGGCCTTTCTCGACAACGGCCTGAACCG
CATCGGCGACAGCGTCGCCCGCCTGACCAACCGCACCCCCAGCCCCGACGAGAACCTGCTGAACGCCACCATCGACATGG
TGGACGGGCTGTCGCGCGCCGCCCGCTTCAAGCAGGCCATCGAGGAGGACGAGACCAAGGCCGAGATCCACGAGCGCCTG
GCCCGGACCCTGGATGGAGAGTTCGGCATCAAGACCTTCTCGCTCTACGAGATCGTGCCCAACAAGAACCAGATGACCCC
GCTGTTCGTGGACGGCGTCCCCGACAGTTCATGCCGTTGGTGCGACCCGCAGATCCTGGTGCGCAGCGAGGGCTGCCGCG
CGCGGCGCACCGGCCACATGGTCGATGGCGTCACCAACCCGGCCATCTGCTACGCCTTCCAGCCGGAAAACGACACCAGC
GGCTACAAGCATATCTGCCTGCCCATCATCCAGTCCGGCTCGGTGGGCAACGTGTTGCAGTTGGTGGCCTCCGAGGGGCA
GGAGCAGCACCTGCTCAGCAAGATTCCCTACGTGAACGTCTATCTGCGCGAGGCCGCTCCGGTGCTGGAGACCAAGCGGT
TGATGGAGACCTTGCGGGAATCGTCGCTCAGCGATCCCATGACCGGGCTGAACAACCGCCGATTCCTCGAAGAATACGTC
GACACCCTGGTGGCCAACGTCCATCGGCGCAAATCCGCCCTGACGGTGATGATGCTGGATCTCGACCACTTCAAGATGGT
CAACGACACCTATGGCCACGATGCCGGCGATGCGGTGCTCAAGGCCCTGTCCAAGGTACTGCGCCAGGCGGTTCGGGCCT
CGGATCTGGTGATCCGCTTCGGCGGCGAGGAATTCCTGATCGTCCTGGTCGACACGCCGTCGGCCGAAGCCGATCAGGTC
GCCGAGAAGATCCGTGCCCTGGTGGAAGCCCTGGTGGTGCAGACGGGAACGGTGACCATCCGCAAGACCATTTCCATCGG
CCTGTCCGACTTCCCCACCGACAGCGACACCTTCTGGCAGGCGGTGAAGTTCGCCGACGTCGCCCTGTACCAGGCCAAGG
AAAATGGCCGCAACCGGGTGGTGCGCTTCAACGAATCCATGTGGGAAAACAAGAAGGAATACTGA

Upstream 100 bases:

>100_bases
GCCTCTCTCCCTGACAGTATTATGACGAGCAATGCACCTAAAGATGCATTGAACATAGATGATAGCAACCAGTGAGTCTT
TTGAATCGGCCGGGGGTCTT

Downstream 100 bases:

>100_bases
GTTCGCGCCGCGGTCTCCACTCTGGCAATGTCAGAGCGGGGATTGCGGCCGGTCAGCTCACCCCTGCCCTTCGCCCTGGA
ACGGCAGGGTGAAGACGAAC

Product: response regulator

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 634; Mature: 633

Protein sequence:

>634_residues
MPIPPAASWGKSRIMSLNSKVTMFFVAMAVSLVFMLVAINLYAFRSFSIASATDHIRTAAEIVRVHLTESMINGVIDKRE
SFLSRLMDVQGLTSAQVVRSPLVEQQFGKGLGRETPNDEVEQQVLHDGKPVYRVEESPNGTIFRGTIPFVASTRGSPNCL
QCHQVQEGAVLGAVTLSMSIEDLKSKALLTVAGIVGIVVLFSAATFFLIRRVIQPVSTTALNVELAVQRALRGDFKGQVS
QRTEDEVGQIAADMNRLLAFLDNGLNRIGDSVARLTNRTPSPDENLLNATIDMVDGLSRAARFKQAIEEDETKAEIHERL
ARTLDGEFGIKTFSLYEIVPNKNQMTPLFVDGVPDSSCRWCDPQILVRSEGCRARRTGHMVDGVTNPAICYAFQPENDTS
GYKHICLPIIQSGSVGNVLQLVASEGQEQHLLSKIPYVNVYLREAAPVLETKRLMETLRESSLSDPMTGLNNRRFLEEYV
DTLVANVHRRKSALTVMMLDLDHFKMVNDTYGHDAGDAVLKALSKVLRQAVRASDLVIRFGGEEFLIVLVDTPSAEADQV
AEKIRALVEALVVQTGTVTIRKTISIGLSDFPTDSDTFWQAVKFADVALYQAKENGRNRVVRFNESMWENKKEY

Sequences:

>Translated_634_residues
MPIPPAASWGKSRIMSLNSKVTMFFVAMAVSLVFMLVAINLYAFRSFSIASATDHIRTAAEIVRVHLTESMINGVIDKRE
SFLSRLMDVQGLTSAQVVRSPLVEQQFGKGLGRETPNDEVEQQVLHDGKPVYRVEESPNGTIFRGTIPFVASTRGSPNCL
QCHQVQEGAVLGAVTLSMSIEDLKSKALLTVAGIVGIVVLFSAATFFLIRRVIQPVSTTALNVELAVQRALRGDFKGQVS
QRTEDEVGQIAADMNRLLAFLDNGLNRIGDSVARLTNRTPSPDENLLNATIDMVDGLSRAARFKQAIEEDETKAEIHERL
ARTLDGEFGIKTFSLYEIVPNKNQMTPLFVDGVPDSSCRWCDPQILVRSEGCRARRTGHMVDGVTNPAICYAFQPENDTS
GYKHICLPIIQSGSVGNVLQLVASEGQEQHLLSKIPYVNVYLREAAPVLETKRLMETLRESSLSDPMTGLNNRRFLEEYV
DTLVANVHRRKSALTVMMLDLDHFKMVNDTYGHDAGDAVLKALSKVLRQAVRASDLVIRFGGEEFLIVLVDTPSAEADQV
AEKIRALVEALVVQTGTVTIRKTISIGLSDFPTDSDTFWQAVKFADVALYQAKENGRNRVVRFNESMWENKKEY
>Mature_633_residues
PIPPAASWGKSRIMSLNSKVTMFFVAMAVSLVFMLVAINLYAFRSFSIASATDHIRTAAEIVRVHLTESMINGVIDKRES
FLSRLMDVQGLTSAQVVRSPLVEQQFGKGLGRETPNDEVEQQVLHDGKPVYRVEESPNGTIFRGTIPFVASTRGSPNCLQ
CHQVQEGAVLGAVTLSMSIEDLKSKALLTVAGIVGIVVLFSAATFFLIRRVIQPVSTTALNVELAVQRALRGDFKGQVSQ
RTEDEVGQIAADMNRLLAFLDNGLNRIGDSVARLTNRTPSPDENLLNATIDMVDGLSRAARFKQAIEEDETKAEIHERLA
RTLDGEFGIKTFSLYEIVPNKNQMTPLFVDGVPDSSCRWCDPQILVRSEGCRARRTGHMVDGVTNPAICYAFQPENDTSG
YKHICLPIIQSGSVGNVLQLVASEGQEQHLLSKIPYVNVYLREAAPVLETKRLMETLRESSLSDPMTGLNNRRFLEEYVD
TLVANVHRRKSALTVMMLDLDHFKMVNDTYGHDAGDAVLKALSKVLRQAVRASDLVIRFGGEEFLIVLVDTPSAEADQVA
EKIRALVEALVVQTGTVTIRKTISIGLSDFPTDSDTFWQAVKFADVALYQAKENGRNRVVRFNESMWENKKEY

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=164, Percent_Identity=39.0243902439024, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1786584, Length=181, Percent_Identity=35.9116022099448, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI1787262, Length=159, Percent_Identity=37.1069182389937, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI87081881, Length=174, Percent_Identity=37.9310344827586, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI87082007, Length=170, Percent_Identity=37.0588235294118, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787816, Length=209, Percent_Identity=33.4928229665072, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI87081974, Length=170, Percent_Identity=32.3529411764706, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1787541, Length=187, Percent_Identity=30.4812834224599, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1787802, Length=143, Percent_Identity=37.0629370629371, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1788381, Length=177, Percent_Identity=31.638418079096, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI87081977, Length=274, Percent_Identity=27.007299270073, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788085, Length=162, Percent_Identity=30.8641975308642, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1788956, Length=167, Percent_Identity=32.9341317365269, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87081921, Length=225, Percent_Identity=25.3333333333333, Blast_Score=66, Evalue=9e-12,
Organism=Escherichia coli, GI1787056, Length=124, Percent_Identity=30.6451612903226, Blast_Score=63, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 70184; Mature: 70053

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIPPAASWGKSRIMSLNSKVTMFFVAMAVSLVFMLVAINLYAFRSFSIASATDHIRTAA
CCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
EIVRVHLTESMINGVIDKRESFLSRLMDVQGLTSAQVVRSPLVEQQFGKGLGRETPNDEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHH
EQQVLHDGKPVYRVEESPNGTIFRGTIPFVASTRGSPNCLQCHQVQEGAVLGAVTLSMSI
HHHHHHCCCCEEEEECCCCCCEEEECCCCEECCCCCCCCHHHHHHHCCCEEEEHEEHHHH
EDLKSKALLTVAGIVGIVVLFSAATFFLIRRVIQPVSTTALNVELAVQRALRGDFKGQVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
QRTEDEVGQIAADMNRLLAFLDNGLNRIGDSVARLTNRTPSPDENLLNATIDMVDGLSRA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
ARFKQAIEEDETKAEIHERLARTLDGEFGIKTFSLYEIVPNKNQMTPLFVDGVPDSSCRW
HHHHHHHHHCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCEEEEECCCCCCCCC
CDPQILVRSEGCRARRTGHMVDGVTNPAICYAFQPENDTSGYKHICLPIIQSGSVGNVLQ
CCCEEEEECCCCCHHHCCCHHCCCCCCEEEEEECCCCCCCCCCEEEHHHCCCCCHHHHHH
LVASEGQEQHLLSKIPYVNVYLREAAPVLETKRLMETLRESSLSDPMTGLNNRRFLEEYV
HHHCCCHHHHHHHHCCEEEEEHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
DTLVANVHRRKSALTVMMLDLDHFKMVNDTYGHDAGDAVLKALSKVLRQAVRASDLVIRF
HHHHHHHHHHHHHEEEEEEECHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEE
GGEEFLIVLVDTPSAEADQVAEKIRALVEALVVQTGTVTIRKTISIGLSDFPTDSDTFWQ
CCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCHHHHH
AVKFADVALYQAKENGRNRVVRFNESMWENKKEY
HHHHHHHHHHHHHCCCCCCEEEECHHHHCCCCCC
>Mature Secondary Structure 
PIPPAASWGKSRIMSLNSKVTMFFVAMAVSLVFMLVAINLYAFRSFSIASATDHIRTAA
CCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
EIVRVHLTESMINGVIDKRESFLSRLMDVQGLTSAQVVRSPLVEQQFGKGLGRETPNDEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHH
EQQVLHDGKPVYRVEESPNGTIFRGTIPFVASTRGSPNCLQCHQVQEGAVLGAVTLSMSI
HHHHHHCCCCEEEEECCCCCCEEEECCCCEECCCCCCCCHHHHHHHCCCEEEEHEEHHHH
EDLKSKALLTVAGIVGIVVLFSAATFFLIRRVIQPVSTTALNVELAVQRALRGDFKGQVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
QRTEDEVGQIAADMNRLLAFLDNGLNRIGDSVARLTNRTPSPDENLLNATIDMVDGLSRA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
ARFKQAIEEDETKAEIHERLARTLDGEFGIKTFSLYEIVPNKNQMTPLFVDGVPDSSCRW
HHHHHHHHHCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCEEEEECCCCCCCCC
CDPQILVRSEGCRARRTGHMVDGVTNPAICYAFQPENDTSGYKHICLPIIQSGSVGNVLQ
CCCEEEEECCCCCHHHCCCHHCCCCCCEEEEEECCCCCCCCCCEEEHHHCCCCCHHHHHH
LVASEGQEQHLLSKIPYVNVYLREAAPVLETKRLMETLRESSLSDPMTGLNNRRFLEEYV
HHHCCCHHHHHHHHCCEEEEEHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
DTLVANVHRRKSALTVMMLDLDHFKMVNDTYGHDAGDAVLKALSKVLRQAVRASDLVIRF
HHHHHHHHHHHHHEEEEEEECHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEE
GGEEFLIVLVDTPSAEADQVAEKIRALVEALVVQTGTVTIRKTISIGLSDFPTDSDTFWQ
CCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCHHHHH
AVKFADVALYQAKENGRNRVVRFNESMWENKKEY
HHHHHHHHHHHHHCCCCCCEEEECHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA