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The map label for this gene is thyA [H]

Identifier: 83310426

GI number: 83310426

Start: 1446472

End: 1447299

Strand: Reverse

Name: thyA [H]

Synonym: amb1327

Alternate gene names: 83310426

Gene position: 1447299-1446472 (Counterclockwise)

Preceding gene: 83310428

Following gene: 83310425

Centisome position: 29.14

GC content: 64.86

Gene sequence:

>828_bases
GTGCCGGCCGCCCCAAATCCGGAGGTTCACCCCTTGCATCAGTACCTGGACCTGCTGCGGCGCATTCAAGCCGAAGGCGT
GACCAAGACCGACCGTACCGGCACCGGCACCACCAGCGTGTTCGGCCACCAGATGCGCTTCGACCTCAGCCGGGGGTTTC
CCCTGGTGACCACCAAGAAGCTCCACACCCGGTCCATCTTCGTCGAGCTGCTGTGGTTTCTGCGGGGCGACACCAATATC
GGCTGGCTGAAGGACAACAAGGTCTCCATCTGGGACGAATGGGCCGACGCCAATGGCGATCTCGGCCCGGTCTACGGCAA
GCAATGGCGGTCCTGGGCCTGCCCCGACGGCAGCACCGTGGATCAGATCACCCAGGTGGTGGAGATGATCCGCAAATCCC
CGGATTCGCGGCGCCTGATCGTCTCGGCCTGGAACCCGGCCGATATCGAGTCCATGGCCCTGCCGCCCTGCCACTGCCTG
TTCCAGTTCTACGTGGCGGACGGAAAGCTGTCGTGCCAGCTTTACCAGCGCTCGGCGGACGTGTTCCTGGGCGTGCCGTT
CAACATCGCCTCCTACGCCCTGCTGACCCACATGGTGGCGCAGGTGACGGGCCTCGAGGTCGGCGCCTTCGTCCACACCT
TCGGCGATGCGCACCTCTATTCCAACCACATGGAGCAGGCGGCGCTGCAATTGTCGCGCGCCCCCCTGCCGCTGCCCCGG
CTGGAGCTCAATCCCGAGGTGAAGGACCTGTTCGCCTTCACCATGGACGACATCAAAGTGGTGGACTACCAGTGCCACGC
CCACATCGCCGCACCGGTGGCGGTATGA

Upstream 100 bases:

>100_bases
TAAGTTTGTTTTCCTTCCTTGTCTCCGACCAAGACGTGACGGCCCGTGGCGAAAGAAGGCGGGGACCCGACATTGCGTCC
CGGTCGGCCGGGCTGGTACA

Downstream 100 bases:

>100_bases
GTCCGACGAGCGGAGCGAGGAGGCAAGCGCGAAGCGCGCCGCCGCTTATGCGGCGGGAGCCAAGGGCTTGGTACAAGCCC
GCCCGGCGATTGAGGGACAA

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MPAAPNPEVHPLHQYLDLLRRIQAEGVTKTDRTGTGTTSVFGHQMRFDLSRGFPLVTTKKLHTRSIFVELLWFLRGDTNI
GWLKDNKVSIWDEWADANGDLGPVYGKQWRSWACPDGSTVDQITQVVEMIRKSPDSRRLIVSAWNPADIESMALPPCHCL
FQFYVADGKLSCQLYQRSADVFLGVPFNIASYALLTHMVAQVTGLEVGAFVHTFGDAHLYSNHMEQAALQLSRAPLPLPR
LELNPEVKDLFAFTMDDIKVVDYQCHAHIAAPVAV

Sequences:

>Translated_275_residues
MPAAPNPEVHPLHQYLDLLRRIQAEGVTKTDRTGTGTTSVFGHQMRFDLSRGFPLVTTKKLHTRSIFVELLWFLRGDTNI
GWLKDNKVSIWDEWADANGDLGPVYGKQWRSWACPDGSTVDQITQVVEMIRKSPDSRRLIVSAWNPADIESMALPPCHCL
FQFYVADGKLSCQLYQRSADVFLGVPFNIASYALLTHMVAQVTGLEVGAFVHTFGDAHLYSNHMEQAALQLSRAPLPLPR
LELNPEVKDLFAFTMDDIKVVDYQCHAHIAAPVAV
>Mature_274_residues
PAAPNPEVHPLHQYLDLLRRIQAEGVTKTDRTGTGTTSVFGHQMRFDLSRGFPLVTTKKLHTRSIFVELLWFLRGDTNIG
WLKDNKVSIWDEWADANGDLGPVYGKQWRSWACPDGSTVDQITQVVEMIRKSPDSRRLIVSAWNPADIESMALPPCHCLF
QFYVADGKLSCQLYQRSADVFLGVPFNIASYALLTHMVAQVTGLEVGAFVHTFGDAHLYSNHMEQAALQLSRAPLPLPRL
ELNPEVKDLFAFTMDDIKVVDYQCHAHIAAPVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=293, Percent_Identity=47.7815699658703, Blast_Score=270, Evalue=8e-73,
Organism=Escherichia coli, GI1789191, Length=264, Percent_Identity=63.6363636363636, Blast_Score=363, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI71993377, Length=284, Percent_Identity=46.4788732394366, Blast_Score=271, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6324648, Length=280, Percent_Identity=41.4285714285714, Blast_Score=202, Evalue=4e-53,
Organism=Drosophila melanogaster, GI17137556, Length=283, Percent_Identity=53.7102473498233, Blast_Score=293, Evalue=6e-80,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 30845; Mature: 30713

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAAPNPEVHPLHQYLDLLRRIQAEGVTKTDRTGTGTTSVFGHQMRFDLSRGFPLVTTKK
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHEEECCCCCCEEEECH
LHTRSIFVELLWFLRGDTNIGWLKDNKVSIWDEWADANGDLGPVYGKQWRSWACPDGSTV
HHHHHHHHHHHHHHHCCCCCEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCH
DQITQVVEMIRKSPDSRRLIVSAWNPADIESMALPPCHCLFQFYVADGKLSCQLYQRSAD
HHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHCCCHHHHHHHHHHCCCCEEEEEEECCCC
VFLGVPFNIASYALLTHMVAQVTGLEVGAFVHTFGDAHLYSNHMEQAALQLSRAPLPLPR
EEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCC
LELNPEVKDLFAFTMDDIKVVDYQCHAHIAAPVAV
CCCCCCHHHHHHHHHCCEEEEEEEECEEEECCCCC
>Mature Secondary Structure 
PAAPNPEVHPLHQYLDLLRRIQAEGVTKTDRTGTGTTSVFGHQMRFDLSRGFPLVTTKK
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHEEECCCCCCEEEECH
LHTRSIFVELLWFLRGDTNIGWLKDNKVSIWDEWADANGDLGPVYGKQWRSWACPDGSTV
HHHHHHHHHHHHHHHCCCCCEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCH
DQITQVVEMIRKSPDSRRLIVSAWNPADIESMALPPCHCLFQFYVADGKLSCQLYQRSAD
HHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHCCCHHHHHHHHHHCCCCEEEEEEECCCC
VFLGVPFNIASYALLTHMVAQVTGLEVGAFVHTFGDAHLYSNHMEQAALQLSRAPLPLPR
EEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCC
LELNPEVKDLFAFTMDDIKVVDYQCHAHIAAPVAV
CCCCCCHHHHHHHHHCCEEEEEEEECEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA