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The map label for this gene is dhfrIII [H]

Identifier: 83310425

GI number: 83310425

Start: 1445877

End: 1446404

Strand: Reverse

Name: dhfrIII [H]

Synonym: amb1326

Alternate gene names: 83310425

Gene position: 1446404-1445877 (Counterclockwise)

Preceding gene: 83310426

Following gene: 83310424

Centisome position: 29.12

GC content: 68.75

Gene sequence:

>528_bases
TTGGTACAAGCCCGCCCGGCGATTGAGGGACAAGCAATGACCCCCTCCGTCGCCCTCATCGTCGCCGTGGCCGCCAACGG
GGTGATCGGCAAGGACAACGCCCTGCCCTGGCATATTCCCGAGGACCTGAAGTGGTTCAAGGAGAACACCCTGGGCAAGC
CGGTGATCATGGGCCGCAAGACCTGGGAGTCCATCGGCCGCCCTCTCCCCAAGCGCCCCAACATCGTGGTGACGCGCCAG
ACCGGGTGGCGGGCCGAGGGGGCGCATACCGCCCATTCCCTGGACGAAGCCCTGGCCCTGGCGGCCCGGCTGGCGCCCGA
GGCGGCGGAGCTGATGGTGATCGGCGGCAACAGCCTGTTCGCCGAGGCCCTGCCCGGAGCCCGGCGCCTGTATCTGACCG
AGATCGCCCGCGCCTATGAGGGCGACACGATGTTTCCCGCCTTCGAGCGCGGCCTCTGGCATGAAGTTTCGCGGCGCCGC
AACGAGGGCGACCCGGCCTTCGACTTCGTGATTCTCGACCGGGCCTGA

Upstream 100 bases:

>100_bases
CACGCCCACATCGCCGCACCGGTGGCGGTATGAGTCCGACGAGCGGAGCGAGGAGGCAAGCGCGAAGCGCGCCGCCGCTT
ATGCGGCGGGAGCCAAGGGC

Downstream 100 bases:

>100_bases
CCGCAACCTGACAGTCACCGTCTTGTAAAGGTTTCCCTGCTCGACAAAGGCAAGGCGGGCAGGCTATGAGGGGCGCACTC
AACCCGAACCGGAACGGCCG

Product: dihydrofolate reductase

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MVQARPAIEGQAMTPSVALIVAVAANGVIGKDNALPWHIPEDLKWFKENTLGKPVIMGRKTWESIGRPLPKRPNIVVTRQ
TGWRAEGAHTAHSLDEALALAARLAPEAAELMVIGGNSLFAEALPGARRLYLTEIARAYEGDTMFPAFERGLWHEVSRRR
NEGDPAFDFVILDRA

Sequences:

>Translated_175_residues
MVQARPAIEGQAMTPSVALIVAVAANGVIGKDNALPWHIPEDLKWFKENTLGKPVIMGRKTWESIGRPLPKRPNIVVTRQ
TGWRAEGAHTAHSLDEALALAARLAPEAAELMVIGGNSLFAEALPGARRLYLTEIARAYEGDTMFPAFERGLWHEVSRRR
NEGDPAFDFVILDRA
>Mature_175_residues
MVQARPAIEGQAMTPSVALIVAVAANGVIGKDNALPWHIPEDLKWFKENTLGKPVIMGRKTWESIGRPLPKRPNIVVTRQ
TGWRAEGAHTAHSLDEALALAARLAPEAAELMVIGGNSLFAEALPGARRLYLTEIARAYEGDTMFPAFERGLWHEVSRRR
NEGDPAFDFVILDRA

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=153, Percent_Identity=38.562091503268, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI28827793, Length=145, Percent_Identity=39.3103448275862, Blast_Score=80, Evalue=1e-15,
Organism=Escherichia coli, GI1786233, Length=162, Percent_Identity=46.2962962962963, Blast_Score=142, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17505899, Length=146, Percent_Identity=32.1917808219178, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI116008026, Length=143, Percent_Identity=36.3636363636364, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24647458, Length=143, Percent_Identity=36.3636363636364, Blast_Score=77, Evalue=4e-15,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 19246; Mature: 19246

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQARPAIEGQAMTPSVALIVAVAANGVIGKDNALPWHIPEDLKWFKENTLGKPVIMGRK
CCCCCCCCCCCCCCCHHHHHEEHHCCCEECCCCCCCCCCCHHHHHHHHCCCCCCEEECCH
TWESIGRPLPKRPNIVVTRQTGWRAEGAHTAHSLDEALALAARLAPEAAELMVIGGNSLF
HHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHH
AEALPGARRLYLTEIARAYEGDTMFPAFERGLWHEVSRRRNEGDPAFDFVILDRA
HHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECC
>Mature Secondary Structure
MVQARPAIEGQAMTPSVALIVAVAANGVIGKDNALPWHIPEDLKWFKENTLGKPVIMGRK
CCCCCCCCCCCCCCCHHHHHEEHHCCCEECCCCCCCCCCCHHHHHHHHCCCCCCEEECCH
TWESIGRPLPKRPNIVVTRQTGWRAEGAHTAHSLDEALALAARLAPEAAELMVIGGNSLF
HHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHH
AEALPGARRLYLTEIARAYEGDTMFPAFERGLWHEVSRRRNEGDPAFDFVILDRA
HHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]