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The map label for this gene is pyrE

Identifier: 83310413

GI number: 83310413

Start: 1430886

End: 1431578

Strand: Reverse

Name: pyrE

Synonym: amb1314

Alternate gene names: 83310413

Gene position: 1431578-1430886 (Counterclockwise)

Preceding gene: 83310415

Following gene: 83310411

Centisome position: 28.82

GC content: 64.79

Gene sequence:

>693_bases
ATGGCAACCACCGTTTCCCGCACCCCGGAACAGAAGCAGGCGGCGCTGACCACCGCGCGCATCCTGCTGGAAATCAAGGC
GATCAATTTCCGCCCGGAAGAGCCCTACATCCTGACCTCGGGCTGGGCCAGCCCGGTTTACGTGGATTGCCGCAAGGTGA
TCTCGTTCCCGCGCGCCCGCACCAAGATCTGCGAATTGATGACCAAGGCCATCATGCGCGAGGTCGGCTATGAATCCGCC
GAGGCGGTGGCCGGCGGCGAGACGGCGGGCATCCCCTATGCCGCGTGGATTTCCGACCGCATGGGCCTGCCCATGCTCTA
CATCCGCAAGAAGCCCAAGGGCTTTGGCCGCAACGCCCAGATCGAAGGCGACTTCAAGGACGGCGACCGGGTGGTGCTGG
TCGAGGACCTGGCGTCGGACGGCGCCTCCAAGGTCAACTTCGTCAACGCGCTGCGCGAAGCCGGCGCCAAGGTCGACCAT
TCCTTCGTGGTGTTCTTCTACGGCGTCTTCCCCGGCGCCATGAAGAGCCTGGAAGAGATCGGCGTCGACCTGGGCTATCT
GTGCAATTGGTGGGATGTGCTGGAAGTGGTCGAGGAAGAAGGCCAGTTCGACAAGCGCGCCGTCGAGGCCGTCCGCTCCT
TCCTCCACGATCCCGTGGGCTGGTCCAGCCTGCACGGTGGCCGCAGCGCCTGA

Upstream 100 bases:

>100_bases
GGATCACGTCGGACATTTCAGTTCCCTATTCTGTCTTCTTGCAGGAAAGCGCGATTGTGCCTATAGACCCCCTTATCGGC
AAGGCAAGAGGGCTCCCCAC

Downstream 100 bases:

>100_bases
GGTGGAAACGTCCCCTGCCGCCTATGGCGGCAGGGTTAGGCCCCTCCCTCCGGGGAATCAGGATATACTCGATGGCCGCG
CGGCCTATGCCGCGGAAGGC

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MATTVSRTPEQKQAALTTARILLEIKAINFRPEEPYILTSGWASPVYVDCRKVISFPRARTKICELMTKAIMREVGYESA
EAVAGGETAGIPYAAWISDRMGLPMLYIRKKPKGFGRNAQIEGDFKDGDRVVLVEDLASDGASKVNFVNALREAGAKVDH
SFVVFFYGVFPGAMKSLEEIGVDLGYLCNWWDVLEVVEEEGQFDKRAVEAVRSFLHDPVGWSSLHGGRSA

Sequences:

>Translated_230_residues
MATTVSRTPEQKQAALTTARILLEIKAINFRPEEPYILTSGWASPVYVDCRKVISFPRARTKICELMTKAIMREVGYESA
EAVAGGETAGIPYAAWISDRMGLPMLYIRKKPKGFGRNAQIEGDFKDGDRVVLVEDLASDGASKVNFVNALREAGAKVDH
SFVVFFYGVFPGAMKSLEEIGVDLGYLCNWWDVLEVVEEEGQFDKRAVEAVRSFLHDPVGWSSLHGGRSA
>Mature_229_residues
ATTVSRTPEQKQAALTTARILLEIKAINFRPEEPYILTSGWASPVYVDCRKVISFPRARTKICELMTKAIMREVGYESAE
AVAGGETAGIPYAAWISDRMGLPMLYIRKKPKGFGRNAQIEGDFKDGDRVVLVEDLASDGASKVNFVNALREAGAKVDHS
FVVFFYGVFPGAMKSLEEIGVDLGYLCNWWDVLEVVEEEGQFDKRAVEAVRSFLHDPVGWSSLHGGRSA

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=206, Percent_Identity=27.1844660194175, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17508631, Length=140, Percent_Identity=33.5714285714286, Blast_Score=74, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17554964, Length=198, Percent_Identity=28.7878787878788, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI17933654, Length=194, Percent_Identity=29.3814432989691, Blast_Score=81, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 25441; Mature: 25310

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATTVSRTPEQKQAALTTARILLEIKAINFRPEEPYILTSGWASPVYVDCRKVISFPRAR
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCHHH
TKICELMTKAIMREVGYESAEAVAGGETAGIPYAAWISDRMGLPMLYIRKKPKGFGRNAQ
HHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCE
IEGDFKDGDRVVLVEDLASDGASKVNFVNALREAGAKVDHSFVVFFYGVFPGAMKSLEEI
ECCCCCCCCEEEEEECHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCHHHHHHHHHH
GVDLGYLCNWWDVLEVVEEEGQFDKRAVEAVRSFLHDPVGWSSLHGGRSA
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHCCCCCCC
>Mature Secondary Structure 
ATTVSRTPEQKQAALTTARILLEIKAINFRPEEPYILTSGWASPVYVDCRKVISFPRAR
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCHHH
TKICELMTKAIMREVGYESAEAVAGGETAGIPYAAWISDRMGLPMLYIRKKPKGFGRNAQ
HHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCE
IEGDFKDGDRVVLVEDLASDGASKVNFVNALREAGAKVDHSFVVFFYGVFPGAMKSLEEI
ECCCCCCCCEEEEEECHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCHHHHHHHHHH
GVDLGYLCNWWDVLEVVEEEGQFDKRAVEAVRSFLHDPVGWSSLHGGRSA
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA