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The map label for this gene is bluB [H]

Identifier: 83310326

GI number: 83310326

Start: 1303514

End: 1304260

Strand: Reverse

Name: bluB [H]

Synonym: amb1227

Alternate gene names: 83310326

Gene position: 1304260-1303514 (Counterclockwise)

Preceding gene: 83310328

Following gene: 83310325

Centisome position: 26.26

GC content: 69.88

Gene sequence:

>747_bases
ATGACAGATCGGGCCGCCGCCATCCAGGGCGACGACGTCTTGACCGAGGCGGACAGGCTGGGGCTGTACCGGGTGCTGCT
GTCGCGGCGCGACGTGCGCGGCCAGTTCCTGCCCGACCCCATTCCCGACGAGGTGCTGGCCCGCATCCTGGTGGCCGCCC
ACCATGCCCCCTCGGTGGGCTTCATGCAGCCCTGGAGCTTCATCCTGGTGCGCTCGCCCGAAACCCGCGCCCGCATCAAG
GACGCCTTCGCCCAGGCCAACGACGAGGCCGCCCGCATGTTCGAGGGCGAGGCCCAGGCCACCTACCGCTCGCTGAAGCT
GGAAGGCATCCTGGAATCGCCGGTCAACATCTGCGTCACCTGCGATCGTGACCGGGTGGGGCCGGTGGTTCTGGGCCGCA
CCCACATCCCCACCATGGACCTCTATTCCACCGTCTGCGCCGTGGAGAATCTCTGGCTGGCGGCCCGCGCCGAGGGCCTG
GGCCTCGGCTGGGTCTCCATCATTTCCGAGCCGGCCCTGAAGGACATCCTGGGCCTGCCCGAGCGGGTGGTGCCGGTGGC
CTATCTCTGCCTGGGCAAGGTCAGCCACTTCTTCGCCCAGCCCGAGCTGGAAACACGCGGCTGGCGCAAGCGCCTGCCCC
TGGCCGATCTGGTCAGCCTGGACCGTTGGGAAGGCCCCAGGGACGAGGCGCTGGCAACGGAATTGGCCGCCGCCCAGGAC
CGGGCGGAACGGGGCGAGGCCTTCTGA

Upstream 100 bases:

>100_bases
CCGTAGCCGAATACCCAGAGATCGCAATCCTTGGTCATGGAAGATCGTGGCGCTAAAGTGACCCCCGCCTCCGGGCCGGG
GCGAGGAAAGGGAGCGAAGA

Downstream 100 bases:

>100_bases
TACTTCCCCTGAAACGGGCGTTTGCGCCGCAGCAAATAGTACTATAACTTAGGCATCGCAATCGCTGCGAAGAGGGGGGA
GCGAGATGCCACCGAAAGGA

Product: nitroreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MTDRAAAIQGDDVLTEADRLGLYRVLLSRRDVRGQFLPDPIPDEVLARILVAAHHAPSVGFMQPWSFILVRSPETRARIK
DAFAQANDEAARMFEGEAQATYRSLKLEGILESPVNICVTCDRDRVGPVVLGRTHIPTMDLYSTVCAVENLWLAARAEGL
GLGWVSIISEPALKDILGLPERVVPVAYLCLGKVSHFFAQPELETRGWRKRLPLADLVSLDRWEGPRDEALATELAAAQD
RAERGEAF

Sequences:

>Translated_248_residues
MTDRAAAIQGDDVLTEADRLGLYRVLLSRRDVRGQFLPDPIPDEVLARILVAAHHAPSVGFMQPWSFILVRSPETRARIK
DAFAQANDEAARMFEGEAQATYRSLKLEGILESPVNICVTCDRDRVGPVVLGRTHIPTMDLYSTVCAVENLWLAARAEGL
GLGWVSIISEPALKDILGLPERVVPVAYLCLGKVSHFFAQPELETRGWRKRLPLADLVSLDRWEGPRDEALATELAAAQD
RAERGEAF
>Mature_247_residues
TDRAAAIQGDDVLTEADRLGLYRVLLSRRDVRGQFLPDPIPDEVLARILVAAHHAPSVGFMQPWSFILVRSPETRARIKD
AFAQANDEAARMFEGEAQATYRSLKLEGILESPVNICVTCDRDRVGPVVLGRTHIPTMDLYSTVCAVENLWLAARAEGLG
LGWVSIISEPALKDILGLPERVVPVAYLCLGKVSHFFAQPELETRGWRKRLPLADLVSLDRWEGPRDEALATELAAAQDR
AERGEAF

Specific function: Maybe catalyzes the reduction of Co(2+) to Co(+) in cob(II)yrinic acid A,C-diamide to allow adenosylation to procede [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012825
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: =1.16.8.1 [H]

Molecular weight: Translated: 27468; Mature: 27337

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDRAAAIQGDDVLTEADRLGLYRVLLSRRDVRGQFLPDPIPDEVLARILVAAHHAPSVG
CCCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC
FMQPWSFILVRSPETRARIKDAFAQANDEAARMFEGEAQATYRSLKLEGILESPVNICVT
CCCCCEEEEEECCCHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEE
CDRDRVGPVVLGRTHIPTMDLYSTVCAVENLWLAARAEGLGLGWVSIISEPALKDILGLP
ECCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHCCC
ERVVPVAYLCLGKVSHFFAQPELETRGWRKRLPLADLVSLDRWEGPRDEALATELAAAQD
HHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHH
RAERGEAF
HHHCCCCC
>Mature Secondary Structure 
TDRAAAIQGDDVLTEADRLGLYRVLLSRRDVRGQFLPDPIPDEVLARILVAAHHAPSVG
CCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC
FMQPWSFILVRSPETRARIKDAFAQANDEAARMFEGEAQATYRSLKLEGILESPVNICVT
CCCCCEEEEEECCCHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEE
CDRDRVGPVVLGRTHIPTMDLYSTVCAVENLWLAARAEGLGLGWVSIISEPALKDILGLP
ECCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHHHHCCC
ERVVPVAYLCLGKVSHFFAQPELETRGWRKRLPLADLVSLDRWEGPRDEALATELAAAQD
HHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHH
RAERGEAF
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7635831; 9256491 [H]