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The map label for this gene is ycbL [H]

Identifier: 83310307

GI number: 83310307

Start: 1280850

End: 1281524

Strand: Reverse

Name: ycbL [H]

Synonym: amb1208

Alternate gene names: 83310307

Gene position: 1281524-1280850 (Counterclockwise)

Preceding gene: 83310309

Following gene: 83310305

Centisome position: 25.8

GC content: 66.96

Gene sequence:

>675_bases
ATGGACGAGACCAGAGTCATGACCCTTCAGCAGTTCGGCGATTTCGGCGATTTCTCCCTGTCGGTCCTGGTGACCAGCCC
GCCCTGGTACGAGAACTGCTATCTCGTGGTGCACAAGCCGACCAATACCCTGGCCATCGTCGATCCCGGCGGAGACGGAG
ACCGGATCCTGGAAGCGGTGGCCGCCACCGGCGCCAAGCCCGAGGTGATCTGGCTGACCCACGGTCATCCCGACCATCTG
GGCGCGGCCCATCAGATCGAGACGACGCTGGGCATCTCCACCCGCGCCCATGCCGACGAGACCCAGGTGATCTCCACCTC
CAGCGATCTCAACCGTACCTTCACCGGCCAGCCGCAAAAGGGCCCGGGCAGCCTGCAGACCTTCACGGGCGAACCCACCG
AAAGCCTGGGCGGTGCCCCGGTGCGGGTGATCCATACCCCCGGCCATACGCCCGGCGGCATCTGCCTGGATTTCGGCGGC
TTCGTGCTGACCGGCGACACCCTGTTCCGCAACGGCGTAGGCCGCACCGACCTGCCGGGCGGCAGCGAACAGCAATTGTG
GGCCTCCATCAACCGCCTGCTGGGCCTGCTGGACGACGATGCCATGCTGTTCTCGGGCCATGGCCCGGAATGGGCGGTGC
GGGAAGCCCGCCGCTGGTGGCGGATGGTGGGATAG

Upstream 100 bases:

>100_bases
GCCAAGCTTCAGTGTCCACCCGGCACCCTTACCATGCCGTAAAGGCGAAAGCATTGCTTGGAAAGCGTGGCCGGCTTCGC
TATAGGGGAGGGAACTTATC

Downstream 100 bases:

>100_bases
AGTTTTTCGCAAATACAGTTTCAGGCTGACAGGCTGCGGAACAGCCCGCTTGCGCGGCAGGGCGACCGCCCTGACCCGTT
CTTTAAAAAGCTGAAGGGTT

Product: Zn-dependent hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MDETRVMTLQQFGDFGDFSLSVLVTSPPWYENCYLVVHKPTNTLAIVDPGGDGDRILEAVAATGAKPEVIWLTHGHPDHL
GAAHQIETTLGISTRAHADETQVISTSSDLNRTFTGQPQKGPGSLQTFTGEPTESLGGAPVRVIHTPGHTPGGICLDFGG
FVLTGDTLFRNGVGRTDLPGGSEQQLWASINRLLGLLDDDAMLFSGHGPEWAVREARRWWRMVG

Sequences:

>Translated_224_residues
MDETRVMTLQQFGDFGDFSLSVLVTSPPWYENCYLVVHKPTNTLAIVDPGGDGDRILEAVAATGAKPEVIWLTHGHPDHL
GAAHQIETTLGISTRAHADETQVISTSSDLNRTFTGQPQKGPGSLQTFTGEPTESLGGAPVRVIHTPGHTPGGICLDFGG
FVLTGDTLFRNGVGRTDLPGGSEQQLWASINRLLGLLDDDAMLFSGHGPEWAVREARRWWRMVG
>Mature_224_residues
MDETRVMTLQQFGDFGDFSLSVLVTSPPWYENCYLVVHKPTNTLAIVDPGGDGDRILEAVAATGAKPEVIWLTHGHPDHL
GAAHQIETTLGISTRAHADETQVISTSSDLNRTFTGQPQKGPGSLQTFTGEPTESLGGAPVRVIHTPGHTPGGICLDFGG
FVLTGDTLFRNGVGRTDLPGGSEQQLWASINRLLGLLDDDAMLFSGHGPEWAVREARRWWRMVG

Specific function: Unknown

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Escherichia coli, GI1787158, Length=184, Percent_Identity=34.7826086956522, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1786406, Length=185, Percent_Identity=30.8108108108108, Blast_Score=67, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: NA

Molecular weight: Translated: 24154; Mature: 24154

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDETRVMTLQQFGDFGDFSLSVLVTSPPWYENCYLVVHKPTNTLAIVDPGGDGDRILEAV
CCCCEEEEHHHCCCCCCEEEEEEEECCCCCCCEEEEEECCCCEEEEEECCCCHHHHHHHH
AATGAKPEVIWLTHGHPDHLGAAHQIETTLGISTRAHADETQVISTSSDLNRTFTGQPQK
HHCCCCCEEEEEECCCCHHCCCCCEEHHEECCCCCCCCCCCEEEECCCCCCCEECCCCCC
GPGSLQTFTGEPTESLGGAPVRVIHTPGHTPGGICLDFGGFVLTGDTLFRNGVGRTDLPG
CCCCCEEECCCCCHHCCCCCEEEEECCCCCCCCEEEECCCEEEECCHHHHCCCCCCCCCC
GSEQQLWASINRLLGLLDDDAMLFSGHGPEWAVREARRWWRMVG
CCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MDETRVMTLQQFGDFGDFSLSVLVTSPPWYENCYLVVHKPTNTLAIVDPGGDGDRILEAV
CCCCEEEEHHHCCCCCCEEEEEEEECCCCCCCEEEEEECCCCEEEEEECCCCHHHHHHHH
AATGAKPEVIWLTHGHPDHLGAAHQIETTLGISTRAHADETQVISTSSDLNRTFTGQPQK
HHCCCCCEEEEEECCCCHHCCCCCEEHHEECCCCCCCCCCCEEEECCCCCCCEECCCCCC
GPGSLQTFTGEPTESLGGAPVRVIHTPGHTPGGICLDFGGFVLTGDTLFRNGVGRTDLPG
CCCCCEEECCCCCHHCCCCCEEEEECCCCCCCCEEEECCCEEEECCHHHHCCCCCCCCCC
GSEQQLWASINRLLGLLDDDAMLFSGHGPEWAVREARRWWRMVG
CCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]