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The map label for this gene is phnD [H]

Identifier: 83310204

GI number: 83310204

Start: 1171645

End: 1172682

Strand: Reverse

Name: phnD [H]

Synonym: amb1105

Alternate gene names: 83310204

Gene position: 1172682-1171645 (Counterclockwise)

Preceding gene: 83310205

Following gene: 83310203

Centisome position: 23.61

GC content: 68.5

Gene sequence:

>1038_bases
ATGGGGATCTCCGTCGCCGTGTGGACGAGGCGAATCGGGCTTTGGGCGCTGCTGATCGGTCAGACGGCCGGACACGTCCA
TGGTGCCGAGGCCCCGCAGGCGCTGCGCTTCGCCCTCATCGCCCCGCAATCCCGGCAGGAAATGGAAGCCAATTGGGGGC
CGGTGCTGGCCGCCCTGGGCAAGCATCTTGGCCGGCCGGTGATGGCCGAGGTCTCGGCCGATTATGCCGGGGCCATCTGG
AATCTGCGGTCCGGCCGGGCGCAACTGGCCTGGCTGGGCAACAAGACCGCCATCGAGGCCGTGGACCTTGCCGGGACGGA
GGTATTCGCCAAGGAGACCTATCCCTCGGGCCTGGGCGGCTATTACACCTATCTGATCGTCCCCCGGGATTCGCCCCTGG
CCAGTGCCGACGAGGTCATCGCCAAAGCCGTGGATTTGACCTTCGCCCAAGGGGACGTCAACTCGACCTCGGGGACGGTG
GTTCCCGGCTATTACCTTTTCGCCAGCCGGCATCTGGAACCCCGCAAGCTGTTCAAGCGCGTGGTCCACGCCAATCACGA
GGACAACATCCGCGCGGTGGCCGAGGGGCGTAGCGACGCCGCCACCGTGGCCAGCCTGATCTACGACCAGCTTGGGCGAC
GCGACCCCGGCACCCTCGCCGCCACCCGGATCGTCTGGCGCTCGCCACTCATTCCCGCCGATCCCCTGGTCCGGCGCCGC
GACCTGCCCGCCGCGGACAAGGAGCGGATCGCCGCGTTCTTCTTCGGCTACGGCCAGCCGGCCGCCGGCAAGTCCGCCAT
GCAACTGGCCGAGGAGCGCAGGGCGATGGACCGCCTCGACATCAGCAGCTTCGTCCCCTCCGACGATGGTCAACTGGTCT
CGGTGCGGCTGATCGAGGCGGCCCGGCAACGGACGCAGACCGAGACCGACACCACGCTGCCGCCGGGCGAACGCCAGCGG
CGGCTGGCCGATATCAACCGCCGTATCGCCCAATTGGAAGGCCGCTCCGGCGCCATGGAAAGCGGGGGAGGACAATGA

Upstream 100 bases:

>100_bases
CTCCGGCAGGTTAATCGAACATTAACCCGCCGGCCCGCCGCCCCGCGCTATTGTTGCGCCGCAGCATGCCGCTGCTGCTT
CCTCGATGAAAGGGATGGCC

Downstream 100 bases:

>100_bases
CCATGCCCTTGGCCCATCGAACGCCGAAATCCATCGGCCGCAAGCTTCTGCTGATCGTTCTGCCGTCCCTGCTGTTCGGC
ACGGTGGTGATGTTCTCGGC

Product: ABC-type phosphate/phosphonate transport system

Products: 3-phospho-serine [Cytoplasm]; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MGISVAVWTRRIGLWALLIGQTAGHVHGAEAPQALRFALIAPQSRQEMEANWGPVLAALGKHLGRPVMAEVSADYAGAIW
NLRSGRAQLAWLGNKTAIEAVDLAGTEVFAKETYPSGLGGYYTYLIVPRDSPLASADEVIAKAVDLTFAQGDVNSTSGTV
VPGYYLFASRHLEPRKLFKRVVHANHEDNIRAVAEGRSDAATVASLIYDQLGRRDPGTLAATRIVWRSPLIPADPLVRRR
DLPAADKERIAAFFFGYGQPAAGKSAMQLAEERRAMDRLDISSFVPSDDGQLVSVRLIEAARQRTQTETDTTLPPGERQR
RLADINRRIAQLEGRSGAMESGGGQ

Sequences:

>Translated_345_residues
MGISVAVWTRRIGLWALLIGQTAGHVHGAEAPQALRFALIAPQSRQEMEANWGPVLAALGKHLGRPVMAEVSADYAGAIW
NLRSGRAQLAWLGNKTAIEAVDLAGTEVFAKETYPSGLGGYYTYLIVPRDSPLASADEVIAKAVDLTFAQGDVNSTSGTV
VPGYYLFASRHLEPRKLFKRVVHANHEDNIRAVAEGRSDAATVASLIYDQLGRRDPGTLAATRIVWRSPLIPADPLVRRR
DLPAADKERIAAFFFGYGQPAAGKSAMQLAEERRAMDRLDISSFVPSDDGQLVSVRLIEAARQRTQTETDTTLPPGERQR
RLADINRRIAQLEGRSGAMESGGGQ
>Mature_344_residues
GISVAVWTRRIGLWALLIGQTAGHVHGAEAPQALRFALIAPQSRQEMEANWGPVLAALGKHLGRPVMAEVSADYAGAIWN
LRSGRAQLAWLGNKTAIEAVDLAGTEVFAKETYPSGLGGYYTYLIVPRDSPLASADEVIAKAVDLTFAQGDVNSTSGTVV
PGYYLFASRHLEPRKLFKRVVHANHEDNIRAVAEGRSDAATVASLIYDQLGRRDPGTLAATRIVWRSPLIPADPLVRRRD
LPAADKERIAAFFFGYGQPAAGKSAMQLAEERRAMDRLDISSFVPSDDGQLVSVRLIEAARQRTQTETDTTLPPGERQRR
LADINRRIAQLEGRSGAMESGGGQ

Specific function: Part of the binding-protein-dependent transport system for alkylphosphonates [H]

COG id: COG3221

COG function: function code P; ABC-type phosphate/phosphonate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790543, Length=313, Percent_Identity=36.4217252396166, Blast_Score=214, Evalue=9e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017797
- InterPro:   IPR005770 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 37435; Mature: 37304

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGISVAVWTRRIGLWALLIGQTAGHVHGAEAPQALRFALIAPQSRQEMEANWGPVLAALG
CCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHHEEEEECCCCHHHHHCCCHHHHHHHH
KHLGRPVMAEVSADYAGAIWNLRSGRAQLAWLGNKTAIEAVDLAGTEVFAKETYPSGLGG
HHHCCCEEEHHCCCHHHHHEECCCCCEEEEECCCCCCEEEECCCCCCEEECCCCCCCCCC
YYTYLIVPRDSPLASADEVIAKAVDLTFAQGDVNSTSGTVVPGYYLFASRHLEPRKLFKR
EEEEEEEECCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCCCHHHHHHCCCCHHHHHHH
VVHANHEDNIRAVAEGRSDAATVASLIYDQLGRRDPGTLAATRIVWRSPLIPADPLVRRR
HHHCCCCCCCCEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHC
DLPAADKERIAAFFFGYGQPAAGKSAMQLAEERRAMDRLDISSFVPSDDGQLVSVRLIEA
CCCCCCHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHH
ARQRTQTETDTTLPPGERQRRLADINRRIAQLEGRSGAMESGGGQ
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
GISVAVWTRRIGLWALLIGQTAGHVHGAEAPQALRFALIAPQSRQEMEANWGPVLAALG
CCEEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHHEEEEECCCCHHHHHCCCHHHHHHHH
KHLGRPVMAEVSADYAGAIWNLRSGRAQLAWLGNKTAIEAVDLAGTEVFAKETYPSGLGG
HHHCCCEEEHHCCCHHHHHEECCCCCEEEEECCCCCCEEEECCCCCCEEECCCCCCCCCC
YYTYLIVPRDSPLASADEVIAKAVDLTFAQGDVNSTSGTVVPGYYLFASRHLEPRKLFKR
EEEEEEEECCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCCCHHHHHHCCCCHHHHHHH
VVHANHEDNIRAVAEGRSDAATVASLIYDQLGRRDPGTLAATRIVWRSPLIPADPLVRRR
HHHCCCCCCCCEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCHHHHC
DLPAADKERIAAFFFGYGQPAAGKSAMQLAEERRAMDRLDISSFVPSDDGQLVSVRLIEA
CCCCCCHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHH
ARQRTQTETDTTLPPGERQRRLADINRRIAQLEGRSGAMESGGGQ
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]