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The map label for this gene is hpaG [H]

Identifier: 83309906

GI number: 83309906

Start: 864725

End: 865501

Strand: Reverse

Name: hpaG [H]

Synonym: amb0807

Alternate gene names: 83309906

Gene position: 865501-864725 (Counterclockwise)

Preceding gene: 83309907

Following gene: 83309905

Centisome position: 17.42

GC content: 71.81

Gene sequence:

>777_bases
ATGCGTCACGCCCGCATCAACTTCCCCGGCACCGCCGCGCCCATCAGCGGAGCCGTCACCCCCGACGGCACCGGCCTGGA
CCTGGGCGGCCGAATCCTCCCCATCCCCCCGGGCGGCTTCGCGCCCCCGGTGGAGGGCTGGATCTACGGCCCGCTGCTCA
ACGAGCGCTCCACCTGGGAGCGGTTCGGCGACAAGCTCAACGCCGATCCCTACAAGGCGCCGCCCACCGTGCCGGTGCTG
TACTTCAAGCCGCGGAACACCCATCTCGGCCATGGCGGCACGGTGCGGCTGCCCGCCACTGCCGCACGCGTCGAGCTGGG
CGCCTCGCTGGGCCTGGTGTTCGGCCGCCAGACGGCGCGGGCCGCCGAGGCCGAGGCCCTGGACAACGTCACCGGCCTGA
CCATCGTGCTGGACCTGTCGGTGCCCAACGACAGCGTCTATCGCCCGCCGGTGCAGGAAAAGTGCTTCGACGGCGCCTGC
CCGGTCGGCCCCTGGGTGGTGGACAAGGCGGACCTGCCCGCCGACCTCTCCTCCCTGTCCATCCGCACCTATGTCAACGG
CGACAAGGTCGCCGAGCGCCGGCTGGACGACCTCTACCGCTCTCCCGCCAAGCTGGTGGCCGAGGTCACCGACTTCATGG
CCCTGTCGGCAGGCGACGTCCTGACCCTGGGCTATCCGGTGGGCGCGGTGCCCACCGCCGGACGCGGCGACACGGTGCGG
GTCGAGATCGACGGCATCGGCGCCCTGGACTGCACCATCGCGGAGGAATCGGCATGA

Upstream 100 bases:

>100_bases
CCTGGCCTCGGACTTCATCCGCATCGTGGTCCTGGTGTTCTTCCCGCCCATGGCCCTGTGGCTGGTCCACGTCACCGGCT
GATCCCTGAGGAAAGCAATC

Downstream 100 bases:

>100_bases
AGCGCGCCCGAATCGCCTATAACGGCGCCATTCACGACGCCCGAGTCGAGGACGGCAATGTGGTCGTCCTGGCCGACGGC
CGCCGGGTCGCCGAGGAAAG

Product: 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase

Products: NA

Alternate protein names: 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase; HHDD isomerase; 5-carboxymethyl-2-hydroxymuconate Delta-isomerase; 5-carboxymethyl-2-oxo-hex-3-ene-1,7-dioate decarboxylase; 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase; OPET decarboxylase [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MRHARINFPGTAAPISGAVTPDGTGLDLGGRILPIPPGGFAPPVEGWIYGPLLNERSTWERFGDKLNADPYKAPPTVPVL
YFKPRNTHLGHGGTVRLPATAARVELGASLGLVFGRQTARAAEAEALDNVTGLTIVLDLSVPNDSVYRPPVQEKCFDGAC
PVGPWVVDKADLPADLSSLSIRTYVNGDKVAERRLDDLYRSPAKLVAEVTDFMALSAGDVLTLGYPVGAVPTAGRGDTVR
VEIDGIGALDCTIAEESA

Sequences:

>Translated_258_residues
MRHARINFPGTAAPISGAVTPDGTGLDLGGRILPIPPGGFAPPVEGWIYGPLLNERSTWERFGDKLNADPYKAPPTVPVL
YFKPRNTHLGHGGTVRLPATAARVELGASLGLVFGRQTARAAEAEALDNVTGLTIVLDLSVPNDSVYRPPVQEKCFDGAC
PVGPWVVDKADLPADLSSLSIRTYVNGDKVAERRLDDLYRSPAKLVAEVTDFMALSAGDVLTLGYPVGAVPTAGRGDTVR
VEIDGIGALDCTIAEESA
>Mature_258_residues
MRHARINFPGTAAPISGAVTPDGTGLDLGGRILPIPPGGFAPPVEGWIYGPLLNERSTWERFGDKLNADPYKAPPTVPVL
YFKPRNTHLGHGGTVRLPATAARVELGASLGLVFGRQTARAAEAEALDNVTGLTIVLDLSVPNDSVYRPPVQEKCFDGAC
PVGPWVVDKADLPADLSSLSIRTYVNGDKVAERRLDDLYRSPAKLVAEVTDFMALSAGDVLTLGYPVGAVPTAGRGDTVR
VEIDGIGALDCTIAEESA

Specific function: Decarboxylates OPET (5-oxo-pent-3-ene-1,2,5- tricarboxylic acid) into HHDD (2-hydroxy-hept-2,4-diene-1,7- dioate) and isomerizes it to OHED (2-oxo-hept-3-ene-1,7-dioate) [H]

COG id: COG0179

COG function: function code Q; 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAH family [H]

Homologues:

Organism=Homo sapiens, GI156231349, Length=184, Percent_Identity=29.3478260869565, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI40786394, Length=184, Percent_Identity=29.3478260869565, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI66348062, Length=174, Percent_Identity=27.5862068965517, Blast_Score=65, Evalue=7e-11,
Organism=Homo sapiens, GI13654274, Length=177, Percent_Identity=27.1186440677966, Blast_Score=65, Evalue=7e-11,
Organism=Homo sapiens, GI215422413, Length=174, Percent_Identity=27.5862068965517, Blast_Score=64, Evalue=9e-11,
Organism=Escherichia coli, GI1787428, Length=178, Percent_Identity=25.8426966292135, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17557057, Length=173, Percent_Identity=26.5895953757225, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24663695, Length=187, Percent_Identity=24.5989304812834, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI28571789, Length=188, Percent_Identity=26.063829787234, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234
- InterPro:   IPR012684
- InterPro:   IPR012686 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =5.3.3.10; =4.1.1.68 [H]

Molecular weight: Translated: 27195; Mature: 27195

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHARINFPGTAAPISGAVTPDGTGLDLGGRILPIPPGGFAPPVEGWIYGPLLNERSTWE
CCCEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEECCCCCCCHHHH
RFGDKLNADPYKAPPTVPVLYFKPRNTHLGHGGTVRLPATAARVELGASLGLVFGRQTAR
HHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEECCHHHEEECCCCEEEEECCCHHH
AAEAEALDNVTGLTIVLDLSVPNDSVYRPPVQEKCFDGACPVGPWVVDKADLPADLSSLS
HHHHHHHCCCCEEEEEEEEECCCCCCCCCCHHHHHCCCCCCCCCEEEECCCCCCCCCCEE
IRTYVNGDKVAERRLDDLYRSPAKLVAEVTDFMALSAGDVLTLGYPVGAVPTAGRGDTVR
EEEEECCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEE
VEIDGIGALDCTIAEESA
EEECCCCEEEEEECCCCC
>Mature Secondary Structure
MRHARINFPGTAAPISGAVTPDGTGLDLGGRILPIPPGGFAPPVEGWIYGPLLNERSTWE
CCCEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEECCCCCCCHHHH
RFGDKLNADPYKAPPTVPVLYFKPRNTHLGHGGTVRLPATAARVELGASLGLVFGRQTAR
HHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEECCHHHEEECCCCEEEEECCCHHH
AAEAEALDNVTGLTIVLDLSVPNDSVYRPPVQEKCFDGACPVGPWVVDKADLPADLSSLS
HHHHHHHCCCCEEEEEEEEECCCCCCCCCCHHHHHCCCCCCCCCEEEECCCCCCCCCCEE
IRTYVNGDKVAERRLDDLYRSPAKLVAEVTDFMALSAGDVLTLGYPVGAVPTAGRGDTVR
EEEEECCHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEE
VEIDGIGALDCTIAEESA
EEECCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550403 [H]