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The map label for this gene is hpaG [H]

Identifier: 83309905

GI number: 83309905

Start: 863964

End: 864728

Strand: Reverse

Name: hpaG [H]

Synonym: amb0806

Alternate gene names: 83309905

Gene position: 864728-863964 (Counterclockwise)

Preceding gene: 83309906

Following gene: 83309904

Centisome position: 17.41

GC content: 67.19

Gene sequence:

>765_bases
ATGAAGCGCGCCCGAATCGCCTATAACGGCGCCATTCACGACGCCCGAGTCGAGGACGGCAATGTGGTCGTCCTGGCCGA
CGGCCGCCGGGTCGCCGAGGAAAGCGTGGTGTGGCTGCCCCCCGTCGCCCCCGGCACCACCTTCGCCCTGGCCATCAACT
ATGCCGACCACGCCAAGGAGCTGGCCTTCAAGGCGCCGGAGAAGCCGCTGGCCTTCCTCAAGGGGCCGGGCACCTTCGTC
GGCCACCGTGCGGTGACGCGCAAGCCCGCCGACGCCGGCTTCATGCACTACGAGTGCGAGTTGGCGGTAGTGATCGGCCG
CACCGCCCGCGACGTCAAGGCCAAGGACGCCTACGACTTCGTGGCGGGCTACACCGTGGCCAACGACTACGCCATCCGCA
ACTTCCTGCAGAACTACTACCGCCCCAACCTGCGCACCAAGAACCGCGACGGCTGCACCGTTCTCGGGCCGTGGATGGTG
GACCGCGACGACGTAGCCGATCCCATGAGCCTCGACATCTCCACCTTCGTCAACGGCGCCCGGGTCCAGTCCGGCAACAC
GCGGGACATGGTGTTCGGGGTGCCCGCCCTGATCGAATACCTGTCGTCCTTCATGACCCTGTCGCCCGGCGACCTGATCC
TCACCGGCACCCCCGACGGCATCGTCGACGTCAAGCCCGGCGACGAGGTGGTGACCGAGGTCGAGGGCGTCGGACGCCTG
ACCAGTTTCATCGTCGACGACGAGACCTATTTCCAGCGGAGCTGA

Upstream 100 bases:

>100_bases
ATCCGGTGGGCGCGGTGCCCACCGCCGGACGCGGCGACACGGTGCGGGTCGAGATCGACGGCATCGGCGCCCTGGACTGC
ACCATCGCGGAGGAATCGGC

Downstream 100 bases:

>100_bases
ACCCGATGATCAAGCACCTCATCAACGGCCGCCAGGTGGAGAGCGCCTCCACCATCGCCAATCTCAACCCCGCCAACAAC
GAGGTGATCTGCCAGATCGC

Product: 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase

Products: NA

Alternate protein names: 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase; HHDD isomerase; 5-carboxymethyl-2-hydroxymuconate Delta-isomerase; 5-carboxymethyl-2-oxo-hex-3-ene-1,7-dioate decarboxylase; 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase; OPET decarboxylase [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MKRARIAYNGAIHDARVEDGNVVVLADGRRVAEESVVWLPPVAPGTTFALAINYADHAKELAFKAPEKPLAFLKGPGTFV
GHRAVTRKPADAGFMHYECELAVVIGRTARDVKAKDAYDFVAGYTVANDYAIRNFLQNYYRPNLRTKNRDGCTVLGPWMV
DRDDVADPMSLDISTFVNGARVQSGNTRDMVFGVPALIEYLSSFMTLSPGDLILTGTPDGIVDVKPGDEVVTEVEGVGRL
TSFIVDDETYFQRS

Sequences:

>Translated_254_residues
MKRARIAYNGAIHDARVEDGNVVVLADGRRVAEESVVWLPPVAPGTTFALAINYADHAKELAFKAPEKPLAFLKGPGTFV
GHRAVTRKPADAGFMHYECELAVVIGRTARDVKAKDAYDFVAGYTVANDYAIRNFLQNYYRPNLRTKNRDGCTVLGPWMV
DRDDVADPMSLDISTFVNGARVQSGNTRDMVFGVPALIEYLSSFMTLSPGDLILTGTPDGIVDVKPGDEVVTEVEGVGRL
TSFIVDDETYFQRS
>Mature_254_residues
MKRARIAYNGAIHDARVEDGNVVVLADGRRVAEESVVWLPPVAPGTTFALAINYADHAKELAFKAPEKPLAFLKGPGTFV
GHRAVTRKPADAGFMHYECELAVVIGRTARDVKAKDAYDFVAGYTVANDYAIRNFLQNYYRPNLRTKNRDGCTVLGPWMV
DRDDVADPMSLDISTFVNGARVQSGNTRDMVFGVPALIEYLSSFMTLSPGDLILTGTPDGIVDVKPGDEVVTEVEGVGRL
TSFIVDDETYFQRS

Specific function: Decarboxylates OPET (5-oxo-pent-3-ene-1,2,5- tricarboxylic acid) into HHDD (2-hydroxy-hept-2,4-diene-1,7- dioate) and isomerizes it to OHED (2-oxo-hept-3-ene-1,7-dioate) [H]

COG id: COG0179

COG function: function code Q; 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAH family [H]

Homologues:

Organism=Homo sapiens, GI156231349, Length=220, Percent_Identity=40.4545454545455, Blast_Score=146, Evalue=1e-35,
Organism=Homo sapiens, GI40786394, Length=220, Percent_Identity=40.4545454545455, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI215422413, Length=192, Percent_Identity=33.3333333333333, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI13654274, Length=200, Percent_Identity=33.5, Blast_Score=112, Evalue=2e-25,
Organism=Homo sapiens, GI66348062, Length=187, Percent_Identity=33.6898395721925, Blast_Score=110, Evalue=1e-24,
Organism=Escherichia coli, GI1787428, Length=187, Percent_Identity=35.8288770053476, Blast_Score=120, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17557057, Length=181, Percent_Identity=34.8066298342541, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324161, Length=216, Percent_Identity=28.7037037037037, Blast_Score=88, Evalue=1e-18,
Organism=Drosophila melanogaster, GI28572127, Length=226, Percent_Identity=35.8407079646018, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24663695, Length=216, Percent_Identity=34.2592592592593, Blast_Score=127, Evalue=5e-30,
Organism=Drosophila melanogaster, GI28571789, Length=202, Percent_Identity=34.6534653465347, Blast_Score=102, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234
- InterPro:   IPR012684
- InterPro:   IPR012686 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: =5.3.3.10; =4.1.1.68 [H]

Molecular weight: Translated: 27839; Mature: 27839

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRARIAYNGAIHDARVEDGNVVVLADGRRVAEESVVWLPPVAPGTTFALAINYADHAKE
CCCCEEEECCCEEECEECCCCEEEEECCCEECCCCEEEECCCCCCCEEEEEEECHHHHHH
LAFKAPEKPLAFLKGPGTFVGHRAVTRKPADAGFMHYECELAVVIGRTARDVKAKDAYDF
HHHCCCCCCEEEEECCCCEECCHHHCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCCHHH
VAGYTVANDYAIRNFLQNYYRPNLRTKNRDGCTVLGPWMVDRDDVADPMSLDISTFVNGA
HHCEEECCHHHHHHHHHHHHCCCCCCCCCCCCEEECCCEECCCCCCCCCEEEHHHHCCCE
RVQSGNTRDMVFGVPALIEYLSSFMTLSPGDLILTGTPDGIVDVKPGDEVVTEVEGVGRL
EEECCCCCEEEECHHHHHHHHHHHHCCCCCCEEEECCCCCEEEECCCHHHHHHHHHHHHH
TSFIVDDETYFQRS
HHEEECCHHHHCCC
>Mature Secondary Structure
MKRARIAYNGAIHDARVEDGNVVVLADGRRVAEESVVWLPPVAPGTTFALAINYADHAKE
CCCCEEEECCCEEECEECCCCEEEEECCCEECCCCEEEECCCCCCCEEEEEEECHHHHHH
LAFKAPEKPLAFLKGPGTFVGHRAVTRKPADAGFMHYECELAVVIGRTARDVKAKDAYDF
HHHCCCCCCEEEEECCCCEECCHHHCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCCHHH
VAGYTVANDYAIRNFLQNYYRPNLRTKNRDGCTVLGPWMVDRDDVADPMSLDISTFVNGA
HHCEEECCHHHHHHHHHHHHCCCCCCCCCCCCEEECCCEECCCCCCCCCEEEHHHHCCCE
RVQSGNTRDMVFGVPALIEYLSSFMTLSPGDLILTGTPDGIVDVKPGDEVVTEVEGVGRL
EEECCCCCEEEECHHHHHHHHHHHHCCCCCCEEEECCCCCEEEECCCHHHHHHHHHHHHH
TSFIVDDETYFQRS
HHEEECCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA