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The map label for this gene is icaA

Identifier: 82752244

GI number: 82752244

Start: 2697352

End: 2698590

Strand: Direct

Name: icaA

Synonym: SAB2541

Alternate gene names: 82752244

Gene position: 2697352-2698590 (Clockwise)

Preceding gene: 82752236

Following gene: 82752245

Centisome position: 98.35

GC content: 33.82

Gene sequence:

>1239_bases
TTGCAATTTTTTAACTTTTTGCTTTTTTATCCTGTATTTATGTCTATTTACTGGATTGTCGGTTCAATTTATTTCTATTT
TACCAGAGAAATTAGATATTCATTGAACAAGAAGCCTGACATAAATGTGGATGAATTAGAAGGCATTACATTTTTACTTG
CCTGTTATAACGAAAGTGAAACGATTGAAGATACGTTGTCTAATGTTCTGGCACTTAAATACGAGAAAAAAGAAATTATT
ATCATTAATGATGGAAGTTCAGATAATACAGCAGAACTCATCTATAAAATTAAAGAAAATAATGACTTTATTTTCGTCGA
TTTACAAGAAAACAGAGGTAAAGCCAACGCACTCAATCAAGGCATTAAACAGGCTTCATATGATTATGTAATGTGCTTGG
ATGCAGATACTATCGTTGATCAAGATGCACCATATTATATGATTGAAAATTTCAAACATGATCCAAAACTTGGTGCAGTT
ACAGGTAATCCTAGAATTCGAAATAAGAGTTCTATTTTAGGTAAAATTCAAACGATAGAATATGCAAGTTTAATTGGCTG
TATTAAGCGAAGTCATACACTTGCTGGCGCAGTCAATACTATTTCGGGTGTCTTCACTCTATTTAAAAAAAGTGCAGTTG
TCGATGTTGGCTACTGGGATACTGATATGATTACCGAAGATATTGCAGTTTCTTGGAAATTGCATTTACGTGGATATCGT
ATTAAGTATGAACCGCTTGCCATGTGTTGGATGTTGGTTCCAGAAACATTGGGAGGTCTTTGGAAGCAACGCGTGAGATG
GGCTCAAGGGGGACACGAAGTATTACTACGAGACTTTTTTAGCACAATGAAAACAAAAAGGTTTCCTTTATATATTTTGA
TGTTTGAGCAAATCATCTCAATTTTATGGGTATATATAGTGCTTCTATATTTAGGCTATTTGTTCATAACAGCAAACTTC
TTAGACTATACATTTATGACATATAGTTTTTCAATATTTCTACTATCATCATTTACTATGACTTTTATAAACGTTATTCA
ATTTACAGTCGCACTCTTTATTGATAGTCGCTACGAGAAAAAGAATATGGCTGGACTCATATTTGTAAGTTGGTATCCGA
CAGTATACTGGATTATTAACGCAGCAGTAGTTCTTGTCGCATTTCCAAAAGCATTAAAACGTAAGAAAGGTGGTTACGCA
ACATGGTCAAGCCCAGACAGAGGGAATACCCAACGCTAA

Upstream 100 bases:

>100_bases
AAATATTTCCGTTTAATTATAACAACAATCTATTGCAAATTAAAATACTATCAATTACCATATGGCTCACAACCTAACTA
ACGAAAGGTAGGTAAAGAAA

Downstream 100 bases:

>100_bases
AATCATCGCTAAATATGTAAGAGAAACAGCACTTATCGCTATATCGTGTGTCTTTTGGATATATTGTTTAGTTGTTCTAC
TCGTTTATATTGGTACTATA

Product: N-glycosyltransferase

Products: NA

Alternate protein names: PNAG synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm polysaccharide intercellular adhesin synthesis protein IcaA; Biofilm PIA synthesis protein IcaA; Intercellular adhesion protein A; N-acetylglucosaminyltransferase IcaA [H]

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MQFFNFLLFYPVFMSIYWIVGSIYFYFTREIRYSLNKKPDINVDELEGITFLLACYNESETIEDTLSNVLALKYEKKEII
IINDGSSDNTAELIYKIKENNDFIFVDLQENRGKANALNQGIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAV
TGNPRIRNKSSILGKIQTIEYASLIGCIKRSHTLAGAVNTISGVFTLFKKSAVVDVGYWDTDMITEDIAVSWKLHLRGYR
IKYEPLAMCWMLVPETLGGLWKQRVRWAQGGHEVLLRDFFSTMKTKRFPLYILMFEQIISILWVYIVLLYLGYLFITANF
LDYTFMTYSFSIFLLSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYPTVYWIINAAVVLVAFPKALKRKKGGYA
TWSSPDRGNTQR

Sequences:

>Translated_412_residues
MQFFNFLLFYPVFMSIYWIVGSIYFYFTREIRYSLNKKPDINVDELEGITFLLACYNESETIEDTLSNVLALKYEKKEII
IINDGSSDNTAELIYKIKENNDFIFVDLQENRGKANALNQGIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAV
TGNPRIRNKSSILGKIQTIEYASLIGCIKRSHTLAGAVNTISGVFTLFKKSAVVDVGYWDTDMITEDIAVSWKLHLRGYR
IKYEPLAMCWMLVPETLGGLWKQRVRWAQGGHEVLLRDFFSTMKTKRFPLYILMFEQIISILWVYIVLLYLGYLFITANF
LDYTFMTYSFSIFLLSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYPTVYWIINAAVVLVAFPKALKRKKGGYA
TWSSPDRGNTQR
>Mature_412_residues
MQFFNFLLFYPVFMSIYWIVGSIYFYFTREIRYSLNKKPDINVDELEGITFLLACYNESETIEDTLSNVLALKYEKKEII
IINDGSSDNTAELIYKIKENNDFIFVDLQENRGKANALNQGIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAV
TGNPRIRNKSSILGKIQTIEYASLIGCIKRSHTLAGAVNTISGVFTLFKKSAVVDVGYWDTDMITEDIAVSWKLHLRGYR
IKYEPLAMCWMLVPETLGGLWKQRVRWAQGGHEVLLRDFFSTMKTKRFPLYILMFEQIISILWVYIVLLYLGYLFITANF
LDYTFMTYSFSIFLLSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYPTVYWIINAAVVLVAFPKALKRKKGGYA
TWSSPDRGNTQR

Specific function: N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N-acetylglucosamine to produce the linear homopolymer poly-beta-1,6-N-acetyl-D- glucosamine (PNAG, also referred to as PIA), a biofilm adhesin polysaccharide.

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787259, Length=414, Percent_Identity=39.3719806763285, Blast_Score=340, Evalue=7e-95,
Organism=Escherichia coli, GI1786560, Length=215, Percent_Identity=31.6279069767442, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 47779; Mature: 47779

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFFNFLLFYPVFMSIYWIVGSIYFYFTREIRYSLNKKPDINVDELEGITFLLACYNESE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCEEEEEEECCCCC
TIEDTLSNVLALKYEKKEIIIINDGSSDNTAELIYKIKENNDFIFVDLQENRGKANALNQ
HHHHHHHHHHEEEECCCEEEEEECCCCCCCEEEEEEEECCCCEEEEEEECCCCCHHHHHH
GIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAVTGNPRIRNKSSILGKIQTIE
HHHHCCCCEEEEECCHHHCCCCCCCHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHH
YASLIGCIKRSHTLAGAVNTISGVFTLFKKSAVVDVGYWDTDMITEDIAVSWKLHLRGYR
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHEEEEEEEEEEEE
IKYEPLAMCWMLVPETLGGLWKQRVRWAQGGHEVLLRDFFSTMKTKRFPLYILMFEQIIS
EEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
ILWVYIVLLYLGYLFITANFLDYTFMTYSFSIFLLSSFTMTFINVIQFTVALFIDSRYEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KNMAGLIFVSWYPTVYWIINAAVVLVAFPKALKRKKGGYATWSSPDRGNTQR
CCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQFFNFLLFYPVFMSIYWIVGSIYFYFTREIRYSLNKKPDINVDELEGITFLLACYNESE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCEEEEEEECCCCC
TIEDTLSNVLALKYEKKEIIIINDGSSDNTAELIYKIKENNDFIFVDLQENRGKANALNQ
HHHHHHHHHHEEEECCCEEEEEECCCCCCCEEEEEEEECCCCEEEEEEECCCCCHHHHHH
GIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAVTGNPRIRNKSSILGKIQTIE
HHHHCCCCEEEEECCHHHCCCCCCCHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHH
YASLIGCIKRSHTLAGAVNTISGVFTLFKKSAVVDVGYWDTDMITEDIAVSWKLHLRGYR
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHEEEEEEEEEEEE
IKYEPLAMCWMLVPETLGGLWKQRVRWAQGGHEVLLRDFFSTMKTKRFPLYILMFEQIIS
EEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
ILWVYIVLLYLGYLFITANFLDYTFMTYSFSIFLLSSFTMTFINVIQFTVALFIDSRYEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KNMAGLIFVSWYPTVYWIINAAVVLVAFPKALKRKKGGYATWSSPDRGNTQR
CCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10496925 [H]