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The map label for this gene is cysJ

Identifier: 82752200

GI number: 82752200

Start: 2634305

End: 2636185

Strand: Reverse

Name: cysJ

Synonym: SAB2494c

Alternate gene names: 82752200

Gene position: 2636185-2634305 (Counterclockwise)

Preceding gene: 82752201

Following gene: 82752199

Centisome position: 96.12

GC content: 34.98

Gene sequence:

>1881_bases
TTGAAATTAAATACATCTAATAGTCCATTTACAGAAAAACAAGTCACAGAGATTAACAATCTGCTGCAAACATTAACAGA
GAGCCAACAACAGTGGCTTAGTGGTTTTTTACTAGCTAAGAGTAACGATACGACAAGTGATAGTAATCAACAACAATTAG
AGACAGAAGTGTGGCAACAATCACAAATATCAGAAGAACAAGCAACTTCAACAACGTATATGTTACAAAATAAAGAGCCA
CATATCGAAGCTAATCAGCGGCATGTCACATTGCTATATGGTTCTGAATCAGGTAATGCCATGCGTTTAGCTGAAATTTT
TTCAGAACGTTTAAGTGATATCGGACATCAAGTTGTTGTGATGTCAATGGATGAATATGATACGACAAACATCGCACAGT
TAGAAGATTTATTTATTATTACGTCTACTCATGGTGAAGGAGAACCGCCTGATAATGCATGGGATTTCTTTGAATTTTTA
GAAGACGATAACGCACCTAATTTAAATCATGTGAGATATTCAGTACTAGCTTTAGGTGATCAAACATATGAATTTTTCTG
TCAAGCAGGTAAAGATGTAGATGCTTTACTAGAAAATCTAGGCGCTGAGCGTATATGTAAGCGTGTAGATTGTGATATTG
ATTATGAAGAAGACGCAGAAAAGTGGATGGCAGACATCATTAATATTATTGATACCACATCAGAAGGTATTCAAAGTGAA
TCGGTGATAAGTGAATCAATTAAGTCTGCCAAAGAAAAGAAATATTCTAAATCAAATCCATACCAAGCAGAAGTTTTAGC
GAATATCAATTTAAATGGTACCGATTCAAATAAAGAAACACGACATATAGAATTTTTACTTGATGATTTTAGTGAATCAT
ATGAACCAGGAGATTGCATAGTAGCATTACCGCAAAACGACCCTGAATTGGTTGAAAAACTAATATCCATGTTAGGTTGG
GATCCGCAATCTCCGGTGCCAATTAATGATCATGGTGATACAGTTCCTATTGTTGAAGCACTAACATCACATTTTGAATT
TACTAAATTAACATTGCCATTATTGAAAAATGCAGATATCTATTTTGACAATGAAGAATTATCTGAACGTATTCAAGATG
AGTCATGGGCGCGTGAATATGTTATAAATCGGGACTTTATAGATTTAATAACAGATTTTCCAACTATAGAATTACAGCCT
GATAATATGTATCAAATCCTTAGAAAATTACCACCAAGAGAGTATTCGATTTCTAGTAGTTTTATGGCAACGCCAGATGA
AGTGCATATTACCGTTGGTACGGTTCGTTATCAAGCACATGGACGTGAGAGAAAAGGTGTATGCTCGGTTCATTTTGCTG
AGCGTATTAAACCAGGCGATATAGTACCAATTTATTTGAAGAAAAATCCGAACTTCAAATTTCCGATGAAGCAAGATATA
CCGGTTATTATGATTGGACCAGGTACTGGAATTGCTCCTTTTAGAGCATATTTACAAGAACGTGAAGAACTTGGTATGAC
TGGAAAAACATGGTTGTTCTTTGGTGATCAACACCGTAGTTCTGACTTTTTATATGAAGAAGAAATAGAAGAATGGCTTG
AAAATGGAAACTTAACACGCGTAGATTTAGCATTTTCAAGAGACCAAGAACACAAAGAATATGTACAGCATCGTATAATG
GAAGAAAGTGAACGTTTCAATGAATGGATTGAGCAAGGCGCAGCAATCTATATTTGTGGCGATGAAAAATGTATGGCGAA
AGATGTCCATCAAGCCATTAAAGATGTATTGGTAAAAGAACGTCATATTTCTCAAGAAGAAGCAGAGTTATTATTGCGAC
AAATGAAACAACAACAACGCTATCAACGTGATGTTTATTAA

Upstream 100 bases:

>100_bases
AAGTTTGTTTTAATTTATACCATACATATTAAAAAATTGACTGAACTATCGATAAAAAATGAGTCAATCATCTATTTCAA
GTTTATTGAGGTGAAAATAT

Downstream 100 bases:

>100_bases
CGATTGGTGTTAAATATTTTAAGGTGTAATGATGTAAAATTATATAAAGGATGTTGCTCAACATGAATATGCCATTAATG
CTAGATTTAACAAATAAAAA

Product: sulfite reductase flavoprotein

Products: NA

Alternate protein names: SiR-FP [H]

Number of amino acids: Translated: 626; Mature: 626

Protein sequence:

>626_residues
MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLAKSNDTTSDSNQQQLETEVWQQSQISEEQATSTTYMLQNKEP
HIEANQRHVTLLYGSESGNAMRLAEIFSERLSDIGHQVVVMSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFL
EDDNAPNLNHVRYSVLALGDQTYEFFCQAGKDVDALLENLGAERICKRVDCDIDYEEDAEKWMADIINIIDTTSEGIQSE
SVISESIKSAKEKKYSKSNPYQAEVLANINLNGTDSNKETRHIEFLLDDFSESYEPGDCIVALPQNDPELVEKLISMLGW
DPQSPVPINDHGDTVPIVEALTSHFEFTKLTLPLLKNADIYFDNEELSERIQDESWAREYVINRDFIDLITDFPTIELQP
DNMYQILRKLPPREYSISSSFMATPDEVHITVGTVRYQAHGRERKGVCSVHFAERIKPGDIVPIYLKKNPNFKFPMKQDI
PVIMIGPGTGIAPFRAYLQEREELGMTGKTWLFFGDQHRSSDFLYEEEIEEWLENGNLTRVDLAFSRDQEHKEYVQHRIM
EESERFNEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKERHISQEEAELLLRQMKQQQRYQRDVY

Sequences:

>Translated_626_residues
MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLAKSNDTTSDSNQQQLETEVWQQSQISEEQATSTTYMLQNKEP
HIEANQRHVTLLYGSESGNAMRLAEIFSERLSDIGHQVVVMSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFL
EDDNAPNLNHVRYSVLALGDQTYEFFCQAGKDVDALLENLGAERICKRVDCDIDYEEDAEKWMADIINIIDTTSEGIQSE
SVISESIKSAKEKKYSKSNPYQAEVLANINLNGTDSNKETRHIEFLLDDFSESYEPGDCIVALPQNDPELVEKLISMLGW
DPQSPVPINDHGDTVPIVEALTSHFEFTKLTLPLLKNADIYFDNEELSERIQDESWAREYVINRDFIDLITDFPTIELQP
DNMYQILRKLPPREYSISSSFMATPDEVHITVGTVRYQAHGRERKGVCSVHFAERIKPGDIVPIYLKKNPNFKFPMKQDI
PVIMIGPGTGIAPFRAYLQEREELGMTGKTWLFFGDQHRSSDFLYEEEIEEWLENGNLTRVDLAFSRDQEHKEYVQHRIM
EESERFNEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKERHISQEEAELLLRQMKQQQRYQRDVY
>Mature_626_residues
MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLAKSNDTTSDSNQQQLETEVWQQSQISEEQATSTTYMLQNKEP
HIEANQRHVTLLYGSESGNAMRLAEIFSERLSDIGHQVVVMSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFL
EDDNAPNLNHVRYSVLALGDQTYEFFCQAGKDVDALLENLGAERICKRVDCDIDYEEDAEKWMADIINIIDTTSEGIQSE
SVISESIKSAKEKKYSKSNPYQAEVLANINLNGTDSNKETRHIEFLLDDFSESYEPGDCIVALPQNDPELVEKLISMLGW
DPQSPVPINDHGDTVPIVEALTSHFEFTKLTLPLLKNADIYFDNEELSERIQDESWAREYVINRDFIDLITDFPTIELQP
DNMYQILRKLPPREYSISSSFMATPDEVHITVGTVRYQAHGRERKGVCSVHFAERIKPGDIVPIYLKKNPNFKFPMKQDI
PVIMIGPGTGIAPFRAYLQEREELGMTGKTWLFFGDQHRSSDFLYEEEIEEWLENGNLTRVDLAFSRDQEHKEYVQHRIM
EESERFNEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKERHISQEEAELLLRQMKQQQRYQRDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Homo sapiens, GI127139033, Length=612, Percent_Identity=30.8823529411765, Blast_Score=266, Evalue=6e-71,
Organism=Homo sapiens, GI7657393, Length=597, Percent_Identity=28.4757118927973, Blast_Score=218, Evalue=1e-56,
Organism=Homo sapiens, GI221316705, Length=606, Percent_Identity=28.0528052805281, Blast_Score=211, Evalue=2e-54,
Organism=Homo sapiens, GI10835173, Length=655, Percent_Identity=28.2442748091603, Blast_Score=210, Evalue=3e-54,
Organism=Homo sapiens, GI221316709, Length=597, Percent_Identity=28.3082077051926, Blast_Score=209, Evalue=8e-54,
Organism=Homo sapiens, GI221316707, Length=520, Percent_Identity=29.0384615384615, Blast_Score=198, Evalue=2e-50,
Organism=Homo sapiens, GI40254422, Length=655, Percent_Identity=27.7862595419847, Blast_Score=196, Evalue=4e-50,
Organism=Homo sapiens, GI24041029, Length=599, Percent_Identity=27.3789649415693, Blast_Score=191, Evalue=2e-48,
Organism=Homo sapiens, GI169790956, Length=425, Percent_Identity=28.4705882352941, Blast_Score=152, Evalue=1e-36,
Organism=Homo sapiens, GI169790958, Length=425, Percent_Identity=28.4705882352941, Blast_Score=152, Evalue=1e-36,
Organism=Escherichia coli, GI1789123, Length=539, Percent_Identity=39.3320964749536, Blast_Score=414, Evalue=1e-116,
Organism=Escherichia coli, GI1790181, Length=152, Percent_Identity=32.8947368421053, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1789154, Length=126, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17554134, Length=605, Percent_Identity=30.0826446280992, Blast_Score=253, Evalue=3e-67,
Organism=Caenorhabditis elegans, GI17566446, Length=572, Percent_Identity=26.9230769230769, Blast_Score=171, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17531441, Length=255, Percent_Identity=27.0588235294118, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321832, Length=653, Percent_Identity=26.0336906584992, Blast_Score=167, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6325305, Length=631, Percent_Identity=24.8811410459588, Blast_Score=156, Evalue=9e-39,
Organism=Saccharomyces cerevisiae, GI6321143, Length=390, Percent_Identity=27.4358974358974, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI17137192, Length=612, Percent_Identity=29.9019607843137, Blast_Score=246, Evalue=3e-65,
Organism=Drosophila melanogaster, GI24582192, Length=514, Percent_Identity=29.9610894941634, Blast_Score=206, Evalue=3e-53,
Organism=Drosophila melanogaster, GI24583543, Length=533, Percent_Identity=29.4559099437148, Blast_Score=181, Evalue=9e-46,
Organism=Drosophila melanogaster, GI78706876, Length=533, Percent_Identity=29.4559099437148, Blast_Score=181, Evalue=9e-46,
Organism=Drosophila melanogaster, GI78706872, Length=533, Percent_Identity=29.4559099437148, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24660907, Length=599, Percent_Identity=25.542570951586, Blast_Score=172, Evalue=5e-43,
Organism=Drosophila melanogaster, GI24660903, Length=599, Percent_Identity=25.542570951586, Blast_Score=172, Evalue=5e-43,
Organism=Drosophila melanogaster, GI24647438, Length=410, Percent_Identity=25.609756097561, Blast_Score=96, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1 [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 72186; Mature: 72186

Theoretical pI: Translated: 4.29; Mature: 4.29

Prosite motif: PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLAKSNDTTSDSNQQQLETEVWQQ
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHH
SQISEEQATSTTYMLQNKEPHIEANQRHVTLLYGSESGNAMRLAEIFSERLSDIGHQVVV
HHCCHHHHCCEEEEEECCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEE
MSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFLEDDNAPNLNHVRYSVLALGD
EEECCCCCCCHHEEEEEEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCHHEEEEEEECC
QTYEFFCQAGKDVDALLENLGAERICKRVDCDIDYEEDAEKWMADIINIIDTTSEGIQSE
HHHHHHHHCCCCHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHH
SVISESIKSAKEKKYSKSNPYQAEVLANINLNGTDSNKETRHIEFLLDDFSESYEPGDCI
HHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCEE
VALPQNDPELVEKLISMLGWDPQSPVPINDHGDTVPIVEALTSHFEFTKLTLPLLKNADI
EECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHEEHHHHCCCCE
YFDNEELSERIQDESWAREYVINRDFIDLITDFPTIELQPDNMYQILRKLPPREYSISSS
EECCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEECCCHHHHHHHHCCCCCEECCCC
FMATPDEVHITVGTVRYQAHGRERKGVCSVHFAERIKPGDIVPIYLKKNPNFKFPMKQDI
CCCCCCEEEEEEEEEEEEECCCCCCCEEEEEHHHCCCCCCEEEEEEECCCCCCCCCCCCC
PVIMIGPGTGIAPFRAYLQEREELGMTGKTWLFFGDQHRSSDFLYEEEIEEWLENGNLTR
CEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCEEE
VDLAFSRDQEHKEYVQHRIMEESERFNEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKE
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHH
RHISQEEAELLLRQMKQQQRYQRDVY
HHCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLAKSNDTTSDSNQQQLETEVWQQ
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHH
SQISEEQATSTTYMLQNKEPHIEANQRHVTLLYGSESGNAMRLAEIFSERLSDIGHQVVV
HHCCHHHHCCEEEEEECCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEE
MSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFLEDDNAPNLNHVRYSVLALGD
EEECCCCCCCHHEEEEEEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCCHHEEEEEEECC
QTYEFFCQAGKDVDALLENLGAERICKRVDCDIDYEEDAEKWMADIINIIDTTSEGIQSE
HHHHHHHHCCCCHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHH
SVISESIKSAKEKKYSKSNPYQAEVLANINLNGTDSNKETRHIEFLLDDFSESYEPGDCI
HHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCEE
VALPQNDPELVEKLISMLGWDPQSPVPINDHGDTVPIVEALTSHFEFTKLTLPLLKNADI
EECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHEEHHHHCCCCE
YFDNEELSERIQDESWAREYVINRDFIDLITDFPTIELQPDNMYQILRKLPPREYSISSS
EECCHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEECCCHHHHHHHHCCCCCEECCCC
FMATPDEVHITVGTVRYQAHGRERKGVCSVHFAERIKPGDIVPIYLKKNPNFKFPMKQDI
CCCCCCEEEEEEEEEEEEECCCCCCCEEEEEHHHCCCCCCEEEEEEECCCCCCCCCCCCC
PVIMIGPGTGIAPFRAYLQEREELGMTGKTWLFFGDQHRSSDFLYEEEIEEWLENGNLTR
CEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCEEE
VDLAFSRDQEHKEYVQHRIMEESERFNEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKE
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHH
RHISQEEAELLLRQMKQQQRYQRDVY
HHCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]