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The map label for this gene is thiM

Identifier: 82751694

GI number: 82751694

Start: 2111733

End: 2112524

Strand: Reverse

Name: thiM

Synonym: SAB1976c

Alternate gene names: 82751694

Gene position: 2112524-2111733 (Counterclockwise)

Preceding gene: 82751695

Following gene: 82751693

Centisome position: 77.03

GC content: 35.35

Gene sequence:

>792_bases
ATGAATTATCTAAATAAAATACGTATTGAAAACCCATTAACAATTTGTTATACAAACGATGTAGTTAAAAATTTTACAGC
GAATGGTTTATTAAGTATTGGTGCGAGCCCTGCAATGAGTGAAGCTCCCGAAGAAGCTGAAGAATTTTACAAAGTTGCAC
AAGCGCTATTAATCAATATCGGTACTTTAACAGCACAAAATGAACAAGATATTATTGCGATTGCTCAAACGGCAAATGAG
GCAGGCTTACCTATTGTATTTGACCCTGTAGCTGTTGGTGCTTCTACATATCGAAAGCAATTTTGTAAATTATTATTGAA
ATCAGCGAAAGTATCAGTAATTAAAGGCAATGCATCTGAAATATTAGCGTTGATTGATGATACAGCAACTATGAAAGGTA
CAGATAGTGATGCTTATCTTGATGCGGTTGCAATAGCGAAAAAGGCTTACGCAATATATAAAACTGCAATAGTAATCACA
GGTAAAGAGGACGTTATTGTTCAAGGTGATAAAGCAATCGTATTAGCTAATGGATCTCCATTATTAGCACGAGTAACTGG
AGCTGGTTGTTTATTAGGAGGCATTATTGCTGGATTTTTATTTAGAGAAACAGAACCAGACATAGAAGCGTTAATTGAAG
CGGTAAGCGTATTTAATATTGCTGCTGAGGTAGCTGCTGAAAATGAAAATTGTGGTGGTCCTGGTACGTTTTCACCATTG
TTGCTTGATACGTTATATCATTTAAATGAAACAACCTATCAACAACGGATTTGCATTCAAGAGGTGGAATAA

Upstream 100 bases:

>100_bases
AAAAAGTTTATTTCAATGAGTATACAATATACGCCTGAAATCGGCCGTGGTAGAGGTCCAGTGAATCATTTTGCATATTT
AAAGAAAGAGGGATTAGACG

Downstream 100 bases:

>100_bases
TATGTTTAACCAATCGTATCTAAATGTGTATTTTATTTGTGGGACCTCCAATGTTCCGAGTCATCGAACTATTCATGAAG
TATTAGAAGCAGCATTAAAG

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPEEAEEFYKVAQALLINIGTLTAQNEQDIIAIAQTANE
AGLPIVFDPVAVGASTYRKQFCKLLLKSAKVSVIKGNASEILALIDDTATMKGTDSDAYLDAVAIAKKAYAIYKTAIVIT
GKEDVIVQGDKAIVLANGSPLLARVTGAGCLLGGIIAGFLFRETEPDIEALIEAVSVFNIAAEVAAENENCGGPGTFSPL
LLDTLYHLNETTYQQRICIQEVE

Sequences:

>Translated_263_residues
MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPEEAEEFYKVAQALLINIGTLTAQNEQDIIAIAQTANE
AGLPIVFDPVAVGASTYRKQFCKLLLKSAKVSVIKGNASEILALIDDTATMKGTDSDAYLDAVAIAKKAYAIYKTAIVIT
GKEDVIVQGDKAIVLANGSPLLARVTGAGCLLGGIIAGFLFRETEPDIEALIEAVSVFNIAAEVAAENENCGGPGTFSPL
LLDTLYHLNETTYQQRICIQEVE
>Mature_263_residues
MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPEEAEEFYKVAQALLINIGTLTAQNEQDIIAIAQTANE
AGLPIVFDPVAVGASTYRKQFCKLLLKSAKVSVIKGNASEILALIDDTATMKGTDSDAYLDAVAIAKKAYAIYKTAIVIT
GKEDVIVQGDKAIVLANGSPLLARVTGAGCLLGGIIAGFLFRETEPDIEALIEAVSVFNIAAEVAAENENCGGPGTFSPL
LLDTLYHLNETTYQQRICIQEVE

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788421, Length=250, Percent_Identity=39.2, Blast_Score=160, Evalue=8e-41,
Organism=Saccharomyces cerevisiae, GI6325042, Length=275, Percent_Identity=31.2727272727273, Blast_Score=108, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000417 [H]

Pfam domain/function: PF02110 HK [H]

EC number: =2.7.1.50 [H]

Molecular weight: Translated: 28020; Mature: 28020

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPEEAEEFYKVAQALLINI
CCCCCCEEECCCEEEEEECHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHH
GTLTAQNEQDIIAIAQTANEAGLPIVFDPVAVGASTYRKQFCKLLLKSAKVSVIKGNASE
CCEECCCCCCEEEEEECCCCCCCCEEECHHHHCHHHHHHHHHHHHHHHCCEEEEECCHHH
ILALIDDTATMKGTDSDAYLDAVAIAKKAYAIYKTAIVITGKEDVIVQGDKAIVLANGSP
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEEEEECCCCEEEECCCEEEEECCCC
LLARVTGAGCLLGGIIAGFLFRETEPDIEALIEAVSVFNIAAEVAAENENCGGPGTFSPL
EEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
LLDTLYHLNETTYQQRICIQEVE
HHHHHHHCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPEEAEEFYKVAQALLINI
CCCCCCEEECCCEEEEEECHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHH
GTLTAQNEQDIIAIAQTANEAGLPIVFDPVAVGASTYRKQFCKLLLKSAKVSVIKGNASE
CCEECCCCCCEEEEEECCCCCCCCEEECHHHHCHHHHHHHHHHHHHHHCCEEEEECCHHH
ILALIDDTATMKGTDSDAYLDAVAIAKKAYAIYKTAIVITGKEDVIVQGDKAIVLANGSP
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHEEEEEEEEECCCCEEEECCCEEEEECCCC
LLARVTGAGCLLGGIIAGFLFRETEPDIEALIEAVSVFNIAAEVAAENENCGGPGTFSPL
EEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
LLDTLYHLNETTYQQRICIQEVE
HHHHHHHCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA