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The map label for this gene is thiE

Identifier: 82751693

GI number: 82751693

Start: 2111090

End: 2111731

Strand: Reverse

Name: thiE

Synonym: SAB1975c

Alternate gene names: 82751693

Gene position: 2111731-2111090 (Counterclockwise)

Preceding gene: 82751694

Following gene: 82751692

Centisome position: 77.0

GC content: 35.05

Gene sequence:

>642_bases
ATGTTTAACCAATCGTATCTAAATGTGTATTTTATTTGTGGGACCTCCAATGTTCCGAGTCATCGAACTATTCATGAAGT
ATTAGAAGCAGCATTAAAGGCAGGGATTACACTTTTCCAATTTCGAGAAAAGGGTGAATCAGCTTTAAAAGGAAACGATA
AACTTGTATTAGCTAAAGAATTACAGCATCTCTGTCATCAATATAATGTGCCTTTTATCGTTAATGATGATGTATCCTTA
GCAAAAGAAATTAACGCTGATGGTATACATGTCGGTCAAGATGATGCGAAAGTCAAAGAGATAGCACAATATTTCACTGA
TAAAATTATTGGACTAAGTATTAGTGATTTAGGTGAGTATGCAAAATCTGATTTAACTCATGTTGATTATATAGGTGTTG
GACCAATCTATCCTACGCCGTCGAAACATGACGCGCATACACCAGTAGGTCCAGAAATGATTGCAACATTTAAAGAAATG
AATCCGCAACTACCGATTGTAGCTATTGGCGGTATAAATACTAGTAACGTGGCGCCGATTGTAGAAGCAGGTGCCAATGG
AATTTCAGTCATTTCAGCCATTTCTAAAAGTGAAAATATTGAAAAGACTGTTAATCGATTCAAAGATTTTTTTAATAATT
AG

Upstream 100 bases:

>100_bases
GGTGGTCCTGGTACGTTTTCACCATTGTTGCTTGATACGTTATATCATTTAAATGAAACAACCTATCAACAACGGATTTG
CATTCAAGAGGTGGAATAAT

Downstream 100 bases:

>100_bases
TTAAATTTTTCCACTTATTGTTTCATACGTACTCATTAATGTGATAGAATAAATCCTATGTGAATATAATGATAGAGGTG
GAAAAATGAAGAAAAAAGCG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MFNQSYLNVYFICGTSNVPSHRTIHEVLEAALKAGITLFQFREKGESALKGNDKLVLAKELQHLCHQYNVPFIVNDDVSL
AKEINADGIHVGQDDAKVKEIAQYFTDKIIGLSISDLGEYAKSDLTHVDYIGVGPIYPTPSKHDAHTPVGPEMIATFKEM
NPQLPIVAIGGINTSNVAPIVEAGANGISVISAISKSENIEKTVNRFKDFFNN

Sequences:

>Translated_213_residues
MFNQSYLNVYFICGTSNVPSHRTIHEVLEAALKAGITLFQFREKGESALKGNDKLVLAKELQHLCHQYNVPFIVNDDVSL
AKEINADGIHVGQDDAKVKEIAQYFTDKIIGLSISDLGEYAKSDLTHVDYIGVGPIYPTPSKHDAHTPVGPEMIATFKEM
NPQLPIVAIGGINTSNVAPIVEAGANGISVISAISKSENIEKTVNRFKDFFNN
>Mature_213_residues
MFNQSYLNVYFICGTSNVPSHRTIHEVLEAALKAGITLFQFREKGESALKGNDKLVLAKELQHLCHQYNVPFIVNDDVSL
AKEINADGIHVGQDDAKVKEIAQYFTDKIIGLSISDLGEYAKSDLTHVDYIGVGPIYPTPSKHDAHTPVGPEMIATFKEM
NPQLPIVAIGGINTSNVAPIVEAGANGISVISAISKSENIEKTVNRFKDFFNN

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=175, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6325042, Length=223, Percent_Identity=30.9417040358744, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 23300; Mature: 23300

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNQSYLNVYFICGTSNVPSHRTIHEVLEAALKAGITLFQFREKGESALKGNDKLVLAKE
CCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCHHHCCCCCEEEHHH
LQHLCHQYNVPFIVNDDVSLAKEINADGIHVGQDDAKVKEIAQYFTDKIIGLSISDLGEY
HHHHHHHCCCCEEECCCHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHH
AKSDLTHVDYIGVGPIYPTPSKHDAHTPVGPEMIATFKEMNPQLPIVAIGGINTSNVAPI
HHHCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCHH
VEAGANGISVISAISKSENIEKTVNRFKDFFNN
HHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFNQSYLNVYFICGTSNVPSHRTIHEVLEAALKAGITLFQFREKGESALKGNDKLVLAKE
CCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCHHHCCCCCEEEHHH
LQHLCHQYNVPFIVNDDVSLAKEINADGIHVGQDDAKVKEIAQYFTDKIIGLSISDLGEY
HHHHHHHCCCCEEECCCHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHH
AKSDLTHVDYIGVGPIYPTPSKHDAHTPVGPEMIATFKEMNPQLPIVAIGGINTSNVAPI
HHHCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCHH
VEAGANGISVISAISKSENIEKTVNRFKDFFNN
HHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA