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The map label for this gene is hemL

Identifier: 82751256

GI number: 82751256

Start: 1646651

End: 1647937

Strand: Reverse

Name: hemL

Synonym: SAB1527c

Alternate gene names: 82751256

Gene position: 1647937-1646651 (Counterclockwise)

Preceding gene: 82751257

Following gene: 82751255

Centisome position: 60.09

GC content: 36.83

Gene sequence:

>1287_bases
ATGAGATATACGAAGTCAGAAGAAGCAATGAAGGTTGCTGAAACTTTAATGCCTGGTGGTGTAAATAGTCCAGTACGCGC
ATTTAAATCAGTAGATACACCAGCAATTTTTATGGATCACGGTAAAGGCTCAAAAATTTATGATATCGATGGTAACGAGT
ATATCGACTATGTACTAAGTTGGGGGCCGCTTATTTTAGGACATAGAGACCCTCAAGTTATTAGTCATTTACATGAAGCA
ATTGATAAAGGTACAAGTTTTGGTGCATCAACATTACTTGAAAATAAATTGGCGCAGCTCGTAATTGACCGAGTACCTTC
AATAGAAAAAGTGCGTATGGTGTCATCTGGTACAGAAGCTACATTGGATACTTTAAGATTGGCACGTGGTTATACTGGCA
GAAATAAAATTGTGAAATTTGAAGGTTGCTATCATGGTCATAGTGATTCGTTATTAATCAAAGCTGGGTCTGGGGTGGCA
ACATTAGGATTGCCGGATTCTCCTGGTGTGCCTGAAGGTATTGCTAAAAATACAATTACGGTTCCATACAATGATTTAGA
TGCTCTTAAAATCGCTTTCGAAAAATTTGGAGACGATATTGCTGGTGTAATCGTAGAACCTGTTGCTGGTAATATGGGTG
TCATACCGCCAATTGAAGGTTTTTTACAGGGATTAAGAGATATTACGACTGAATACGGCGCATTGCTAATTTTCGATGAA
GTAATGACTGGTTTCAGAGTCGGTTATCATTGTGCACAAGGTTACTTTGGTGTGACACCAGATTTAACTTGCTTAGGAAA
AGTTATCGGTGGAGGACTACCTGTAGGTGCTTTTGGTGGTAAAAAAGAAATCATGGATCAAATAGCACCATTAGGAAATA
TTTATCAAGCTGGTACGTTATCAGGAAATCCTCTTGCAATGACAAGTGGTTATGAAACGTTAAGTCAATTAGCGCCAGAG
ACATATGAGTATTTTAATATGTTAGGCGATATACTTGAAGACGGTTTAAAGCGTGTATTTGCTAAACACAATGTACCAAT
AACTGTAAATAGAGCAGGTTCAATGATTGGTTATTTCTTAAATGAAGGACCTGTAACTAATTTTGAACAAGCGAATAAAA
GTGATTTGAAATTATTTGCAGAAATGTATCGTGAAATGGCAAAAGAAGGTGTATTTTTACCACCATCTCAATTTGAAGGT
ACATTCTTATCTACGGCACACACGAAAGAAGATATTGAAAAAACGATTCAAGCATTTGATACGGCTTTAAGTCGTATTGT
AAAATAA

Upstream 100 bases:

>100_bases
ATATGATTATTACGTATTTTGCAAAGGACATTTGTCGCTATTTAGATAAATAAGGTTTTATATGTATGATTTTCCATAAA
CTATAGGAGGAATTTACTTT

Downstream 100 bases:

>100_bases
ATATACGGACAAATTGAGAGCCTGAACTTTGTTCAGGCTCTTTTTAAATGTATATCAGGTATGGGCAGCGACTTGATAGT
GAAAGTCCATATATGCTTTG

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA 1; Glutamate-1-semialdehyde aminotransferase 1; GSA-AT 1 [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MRYTKSEEAMKVAETLMPGGVNSPVRAFKSVDTPAIFMDHGKGSKIYDIDGNEYIDYVLSWGPLILGHRDPQVISHLHEA
IDKGTSFGASTLLENKLAQLVIDRVPSIEKVRMVSSGTEATLDTLRLARGYTGRNKIVKFEGCYHGHSDSLLIKAGSGVA
TLGLPDSPGVPEGIAKNTITVPYNDLDALKIAFEKFGDDIAGVIVEPVAGNMGVIPPIEGFLQGLRDITTEYGALLIFDE
VMTGFRVGYHCAQGYFGVTPDLTCLGKVIGGGLPVGAFGGKKEIMDQIAPLGNIYQAGTLSGNPLAMTSGYETLSQLAPE
TYEYFNMLGDILEDGLKRVFAKHNVPITVNRAGSMIGYFLNEGPVTNFEQANKSDLKLFAEMYREMAKEGVFLPPSQFEG
TFLSTAHTKEDIEKTIQAFDTALSRIVK

Sequences:

>Translated_428_residues
MRYTKSEEAMKVAETLMPGGVNSPVRAFKSVDTPAIFMDHGKGSKIYDIDGNEYIDYVLSWGPLILGHRDPQVISHLHEA
IDKGTSFGASTLLENKLAQLVIDRVPSIEKVRMVSSGTEATLDTLRLARGYTGRNKIVKFEGCYHGHSDSLLIKAGSGVA
TLGLPDSPGVPEGIAKNTITVPYNDLDALKIAFEKFGDDIAGVIVEPVAGNMGVIPPIEGFLQGLRDITTEYGALLIFDE
VMTGFRVGYHCAQGYFGVTPDLTCLGKVIGGGLPVGAFGGKKEIMDQIAPLGNIYQAGTLSGNPLAMTSGYETLSQLAPE
TYEYFNMLGDILEDGLKRVFAKHNVPITVNRAGSMIGYFLNEGPVTNFEQANKSDLKLFAEMYREMAKEGVFLPPSQFEG
TFLSTAHTKEDIEKTIQAFDTALSRIVK
>Mature_428_residues
MRYTKSEEAMKVAETLMPGGVNSPVRAFKSVDTPAIFMDHGKGSKIYDIDGNEYIDYVLSWGPLILGHRDPQVISHLHEA
IDKGTSFGASTLLENKLAQLVIDRVPSIEKVRMVSSGTEATLDTLRLARGYTGRNKIVKFEGCYHGHSDSLLIKAGSGVA
TLGLPDSPGVPEGIAKNTITVPYNDLDALKIAFEKFGDDIAGVIVEPVAGNMGVIPPIEGFLQGLRDITTEYGALLIFDE
VMTGFRVGYHCAQGYFGVTPDLTCLGKVIGGGLPVGAFGGKKEIMDQIAPLGNIYQAGTLSGNPLAMTSGYETLSQLAPE
TYEYFNMLGDILEDGLKRVFAKHNVPITVNRAGSMIGYFLNEGPVTNFEQANKSDLKLFAEMYREMAKEGVFLPPSQFEG
TFLSTAHTKEDIEKTIQAFDTALSRIVK

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=318, Percent_Identity=31.7610062893082, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI284507298, Length=238, Percent_Identity=34.0336134453782, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI13994255, Length=328, Percent_Identity=25.3048780487805, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI24119277, Length=357, Percent_Identity=27.4509803921569, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1786349, Length=425, Percent_Identity=52.2352941176471, Blast_Score=463, Evalue=1e-132,
Organism=Escherichia coli, GI1789016, Length=378, Percent_Identity=30.952380952381, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI145693181, Length=321, Percent_Identity=31.4641744548287, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1788044, Length=344, Percent_Identity=31.9767441860465, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1789759, Length=338, Percent_Identity=30.7692307692308, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI1787560, Length=351, Percent_Identity=29.0598290598291, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1786991, Length=286, Percent_Identity=26.5734265734266, Blast_Score=75, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI25144271, Length=313, Percent_Identity=29.073482428115, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71992977, Length=337, Percent_Identity=28.486646884273, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI25144274, Length=161, Percent_Identity=37.2670807453416, Blast_Score=86, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6324432, Length=360, Percent_Identity=27.7777777777778, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6323470, Length=329, Percent_Identity=27.6595744680851, Blast_Score=87, Evalue=7e-18,
Organism=Drosophila melanogaster, GI21357415, Length=318, Percent_Identity=27.0440251572327, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21356575, Length=341, Percent_Identity=27.8592375366569, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =5.4.3.8 [H]

Molecular weight: Translated: 46365; Mature: 46365

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYTKSEEAMKVAETLMPGGVNSPVRAFKSVDTPAIFMDHGKGSKIYDIDGNEYIDYVLS
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEEEEECCCCCEEEECCCCHHHHHHHH
WGPLILGHRDPQVISHLHEAIDKGTSFGASTLLENKLAQLVIDRVPSIEKVRMVSSGTEA
CCCEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHH
TLDTLRLARGYTGRNKIVKFEGCYHGHSDSLLIKAGSGVATLGLPDSPGVPEGIAKNTIT
HHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEECCCEEEECCCCCCCCCCCCCCCEEE
VPYNDLDALKIAFEKFGDDIAGVIVEPVAGNMGVIPPIEGFLQGLRDITTEYGALLIFDE
ECCCCHHHHHHHHHHHCCHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VMTGFRVGYHCAQGYFGVTPDLTCLGKVIGGGLPVGAFGGKKEIMDQIAPLGNIYQAGTL
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCC
SGNPLAMTSGYETLSQLAPETYEYFNMLGDILEDGLKRVFAKHNVPITVNRAGSMIGYFL
CCCCEEEECCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHH
NEGPVTNFEQANKSDLKLFAEMYREMAKEGVFLPPSQFEGTFLSTAHTKEDIEKTIQAFD
CCCCCCCHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHH
TALSRIVK
HHHHHHCC
>Mature Secondary Structure
MRYTKSEEAMKVAETLMPGGVNSPVRAFKSVDTPAIFMDHGKGSKIYDIDGNEYIDYVLS
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCEEEEECCCCCEEEECCCCHHHHHHHH
WGPLILGHRDPQVISHLHEAIDKGTSFGASTLLENKLAQLVIDRVPSIEKVRMVSSGTEA
CCCEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHH
TLDTLRLARGYTGRNKIVKFEGCYHGHSDSLLIKAGSGVATLGLPDSPGVPEGIAKNTIT
HHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEECCCEEEECCCCCCCCCCCCCCCEEE
VPYNDLDALKIAFEKFGDDIAGVIVEPVAGNMGVIPPIEGFLQGLRDITTEYGALLIFDE
ECCCCHHHHHHHHHHHCCHHHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VMTGFRVGYHCAQGYFGVTPDLTCLGKVIGGGLPVGAFGGKKEIMDQIAPLGNIYQAGTL
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCC
SGNPLAMTSGYETLSQLAPETYEYFNMLGDILEDGLKRVFAKHNVPITVNRAGSMIGYFL
CCCCEEEECCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHH
NEGPVTNFEQANKSDLKLFAEMYREMAKEGVFLPPSQFEGTFLSTAHTKEDIEKTIQAFD
CCCCCCCHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHH
TALSRIVK
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA