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The map label for this gene is miaB [H]

Identifier: 82750892

GI number: 82750892

Start: 1253571

End: 1255115

Strand: Direct

Name: miaB [H]

Synonym: SAB1154

Alternate gene names: 82750892

Gene position: 1253571-1255115 (Clockwise)

Preceding gene: 82750891

Following gene: 82750893

Centisome position: 45.71

GC content: 32.88

Gene sequence:

>1545_bases
GTGAACGAAGAACAAAGAAAAGCAAGTTCTGTAGATGTTTTAGCTGAGAGAGATAAGAAAGCAGAAAAAGATTATAGTAA
ATATTTTGAACATGTTTATCAACCGCCTAATTTAAAAGAAGCGAAAAAAAGAGGTAAACAAGAAGTTCGTTACAATAGAG
ATTTCCAAATTGATGAAAAATATCGCGGTATGGGGAACGAGCGTACATTTTTAATTAAAACATATGGATGTCAAATGAAT
GCACATGACACTGAGGTCATTGCTGGTATACTTGAAGCATTAGGCTATCAAGCAACGACTGATATTAACACTGCAGATGT
TATTTTAATTAATACATGTGCGATTAGAGAAAATGCCGAGAACAAAGTGTTTAGTGAAATTGGTAATTTGAAGCATTTGA
AGAAAGAACGACCTGATATTTTAATCGGAGTTTGTGGTTGTATGTCACAAGAAGAGTCAGTAGTGAATAAAATTTTAAAA
TCGTATCAAAATGTAGATATGATTTTTGGTACACATAATATTCATCATTTACCAGAAATTTTAGAAGAAGCATACTTATC
TAAAGCAATGGTTGTTGAAGTATGGTCTAAAGAAGGAGACGTTATTGAAAATCTTCCAAAAGTCCGTGAAGGCAACATTA
AAGCATGGGTCAATATTATGTATGGTTGTGATAAGTTTTGTACATATTGCATTGTTCCATTTACAAGAGGTAAAGAACGA
AGCCGTAGACCTGAAGATATTATAGATGAAGTACGTGAGCTTGCTCGTGAAGGTTACAAAGAAATAACGCTTTTAGGTCA
AAATGTAAATTCATATGGTAAAGATTTACAGGATATAGAATATGACTTAGGAGATCTTTTACAAGCAATTTCTAAAATAG
CGATTCCAAGAGTTCGTTTCACAACTAGTCATCCTTGGGACTTCACAGATCACATGATTGATGTTATTTCAGAGGGCGGT
AATATCGTTCCTCATATCCACTTGCCAGTTCAATCTGGAAATAATGCAGTATTAAAAATAATGGGTAGAAAATATACACG
AGAAAGTTATTTGGATTTAGTAAAACGAATCAAAGATAGAATTCCAAATGTAGCATTAACTACAGATATTATTGTAGGTT
ATCCAAATGAATCAGAGGAACAATTTGAAGAAACTTTAACTTTGTATGATGAAGTTGGTTTTGAACATGCATATACGTAT
TTGTATTCACAACGTGATGGTACGCCTGCTGCTAAAATGAAAGATAATGTACCTTTAGATGTCAAAAAGGAACGATTGCA
ACGTTTGAATAAAAAAGTTGGTCATTATTCACAAATAGCTATGAGTAAGTACGAAGGACAAACTGTAACAGTACTTTGTG
AAGGTAGTAGTAAAAAAGATGATCAGGTTCTTGCTGGCTACACTGATAAAAATAAGTTAGTTAATTTCAAAGCACCTAAA
GAAATGATTGGTAAACTAGTGGAAGTACGAATAGATGAAGCTAAACAGTATTCATTAAATGGCAGTTTTGTAAAGGAAGA
AGAGCCGGAAATGGTGATTCAATAA

Upstream 100 bases:

>100_bases
CTTGTTAGAGATGACGTATAACTTTAACTGTTTTTGCACTTTATAGTTAAATTTAATATAATTATTAAATGATACGGGCA
AATAGAAAGGATTTTGTAAA

Downstream 100 bases:

>100_bases
ATGTATAATAAAGATGACGTGTTGAAACAAGCGGATAATATTGCAAATAAAATTAAAAATTTGGATACTATCAAAACATA
TCAACAAATTGAAGCACAGA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKRGKQEVRYNRDFQIDEKYRGMGNERTFLIKTYGCQMN
AHDTEVIAGILEALGYQATTDINTADVILINTCAIRENAENKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILK
SYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVNIMYGCDKFCTYCIVPFTRGKER
SRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDLQDIEYDLGDLLQAISKIAIPRVRFTTSHPWDFTDHMIDVISEGG
NIVPHIHLPVQSGNNAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTDIIVGYPNESEEQFEETLTLYDEVGFEHAYTY
LYSQRDGTPAAKMKDNVPLDVKKERLQRLNKKVGHYSQIAMSKYEGQTVTVLCEGSSKKDDQVLAGYTDKNKLVNFKAPK
EMIGKLVEVRIDEAKQYSLNGSFVKEEEPEMVIQ

Sequences:

>Translated_514_residues
MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKRGKQEVRYNRDFQIDEKYRGMGNERTFLIKTYGCQMN
AHDTEVIAGILEALGYQATTDINTADVILINTCAIRENAENKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILK
SYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVNIMYGCDKFCTYCIVPFTRGKER
SRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDLQDIEYDLGDLLQAISKIAIPRVRFTTSHPWDFTDHMIDVISEGG
NIVPHIHLPVQSGNNAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTDIIVGYPNESEEQFEETLTLYDEVGFEHAYTY
LYSQRDGTPAAKMKDNVPLDVKKERLQRLNKKVGHYSQIAMSKYEGQTVTVLCEGSSKKDDQVLAGYTDKNKLVNFKAPK
EMIGKLVEVRIDEAKQYSLNGSFVKEEEPEMVIQ
>Mature_514_residues
MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKRGKQEVRYNRDFQIDEKYRGMGNERTFLIKTYGCQMN
AHDTEVIAGILEALGYQATTDINTADVILINTCAIRENAENKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILK
SYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVNIMYGCDKFCTYCIVPFTRGKER
SRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDLQDIEYDLGDLLQAISKIAIPRVRFTTSHPWDFTDHMIDVISEGG
NIVPHIHLPVQSGNNAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTDIIVGYPNESEEQFEETLTLYDEVGFEHAYTY
LYSQRDGTPAAKMKDNVPLDVKKERLQRLNKKVGHYSQIAMSKYEGQTVTVLCEGSSKKDDQVLAGYTDKNKLVNFKAPK
EMIGKLVEVRIDEAKQYSLNGSFVKEEEPEMVIQ

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872782, Length=443, Percent_Identity=37.6975169300226, Blast_Score=288, Evalue=6e-78,
Organism=Homo sapiens, GI28872784, Length=443, Percent_Identity=37.6975169300226, Blast_Score=288, Evalue=7e-78,
Organism=Homo sapiens, GI93277076, Length=443, Percent_Identity=28.6681715575621, Blast_Score=179, Evalue=6e-45,
Organism=Escherichia coli, GI1786882, Length=440, Percent_Identity=41.1363636363636, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI1787057, Length=462, Percent_Identity=25.7575757575758, Blast_Score=153, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17553146, Length=475, Percent_Identity=33.0526315789474, Blast_Score=253, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI71996771, Length=364, Percent_Identity=27.1978021978022, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI21356207, Length=482, Percent_Identity=37.551867219917, Blast_Score=300, Evalue=2e-81,
Organism=Drosophila melanogaster, GI19922432, Length=462, Percent_Identity=30.0865800865801, Blast_Score=189, Evalue=4e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 58949; Mature: 58949

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKRGKQEVRYNRDFQIDEK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHCCCCCCCCCHH
YRGMGNERTFLIKTYGCQMNAHDTEVIAGILEALGYQATTDINTADVILINTCAIRENAE
HCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCH
NKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILKSYQNVDMIFGTHNIHHLPEI
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHH
LEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVNIMYGCDKFCTYCIVPFTRGKER
HHHHHHHHHHHEEECCCCCCHHHHCCCCCCCCHHEEHHHHHHHHHHHHHEEEECCCCCCC
SRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDLQDIEYDLGDLLQAISKIAIPRVRF
CCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEE
TTSHPWDFTDHMIDVISEGGNIVPHIHLPVQSGNNAVLKIMGRKYTRESYLDLVKRIKDR
ECCCCCCHHHHHHHHHHCCCCEEEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHH
IPNVALTTDIIVGYPNESEEQFEETLTLYDEVGFEHAYTYLYSQRDGTPAAKMKDNVPLD
CCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHCHHHHHHHHEECCCCCCHHHCCCCCCCC
VKKERLQRLNKKVGHYSQIAMSKYEGQTVTVLCEGSSKKDDQVLAGYTDKNKLVNFKAPK
HHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEECCCCCCCCCEEECCCCCCCEEECCCCH
EMIGKLVEVRIDEAKQYSLNGSFVKEEEPEMVIQ
HHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKRGKQEVRYNRDFQIDEK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHCCCCCCCCCHH
YRGMGNERTFLIKTYGCQMNAHDTEVIAGILEALGYQATTDINTADVILINTCAIRENAE
HCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCH
NKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILKSYQNVDMIFGTHNIHHLPEI
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHH
LEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVNIMYGCDKFCTYCIVPFTRGKER
HHHHHHHHHHHEEECCCCCCHHHHCCCCCCCCHHEEHHHHHHHHHHHHHEEEECCCCCCC
SRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDLQDIEYDLGDLLQAISKIAIPRVRF
CCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEE
TTSHPWDFTDHMIDVISEGGNIVPHIHLPVQSGNNAVLKIMGRKYTRESYLDLVKRIKDR
ECCCCCCHHHHHHHHHHCCCCEEEEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHH
IPNVALTTDIIVGYPNESEEQFEETLTLYDEVGFEHAYTYLYSQRDGTPAAKMKDNVPLD
CCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHCHHHHHHHHEECCCCCCHHHCCCCCCCC
VKKERLQRLNKKVGHYSQIAMSKYEGQTVTVLCEGSSKKDDQVLAGYTDKNKLVNFKAPK
HHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEECCCCCCCCCEEECCCCCCCEEECCCCH
EMIGKLVEVRIDEAKQYSLNGSFVKEEEPEMVIQ
HHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA